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P06760

- BGLR_RAT

UniProt

P06760 - BGLR_RAT

Protein

Beta-glucuronidase

Gene

Gusb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    Plays an important role in the degradation of dermatan and keratan sulfates.

    Catalytic activityi

    A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

    Enzyme regulationi

    Inhibited by L-aspartic acid.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei447 – 4471Proton donorBy similarity

    GO - Molecular functioni

    1. beta-glucuronidase activity Source: RGD
    2. carbohydrate binding Source: RGD

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKP06760.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucuronidase (EC:3.2.1.31)
    Gene namesi
    Name:Gusb
    Synonyms:Gus
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2772. Gusb.

    Subcellular locationi

    GO - Cellular componenti

    1. lysosome Source: RGD

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Add
    BLAST
    Chaini23 – 648626Beta-glucuronidasePRO_0000012163Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi416 – 4161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi591 – 5911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi627 – 6271N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Undergoes a post-transcriptional proteolytic cleavage near its C-terminal end, which reduces its size by approximately 3 kDa. The site of this cleavage has as yet not been determined.

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP06760.
    PRIDEiP06760.

    PTM databases

    PhosphoSiteiP06760.

    Expressioni

    Gene expression databases

    GenevestigatoriP06760.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    IntActiP06760. 1 interaction.
    STRINGi10116.ENSRNOP00000045033.

    Structurei

    3D structure databases

    ProteinModelPortaliP06760.
    SMRiP06760. Positions 22-628.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000120896.
    HOVERGENiHBG004843.
    KOiK01195.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SUPFAMiSSF49303. SSF49303. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06760-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPRRSVCWF VLGQLLCSCA VALQGGMLFP KETPSRELKV LDGLWSFRAD    50
    YSNNRLQGFE KQWYRQPLRE SGPTLDMPVP SSFNDITQEA ELRNFIGWVW 100
    YEREAVLPQR WTQDTDRRVV LRINSAHYYA VVWVNGIHVV EHEGGHLPFE 150
    ADITKLVQSG PLTTFRVTIA INNTLTPYTL PPGTIVYKTD PSMYPKGYFV 200
    QDISFDFFNY AGLHRSVVLY TTPTTYIDDI TVTTDVDRDV GLVNYWISVQ 250
    GSDHFQLEVR LLDEDGKIVA RGTGNEGQLK VPRAHLWWPY LMHEHPAYLY 300
    SLEVTMTTPE SVSDFYTLPV GIRTVAVTKS KFLINGKPFY FQGVNKHEDS 350
    DIRGRGFDWP LLIKDFNLLR WLGANSFRTS HYPYSEEVLQ LCDRYGIVVI 400
    DECPGVGIVL PQSFGNVSLR HHLEVMDELV RRDKNHPAVV MWSVANEPVS 450
    SLKPAGYYFK TLIAHTKALD PTRPVTFVSN TRYDADMGAP YVDVICVNSY 500
    LSWYHDYGHL EVIQLQLTSQ FENWYKMYQK PIIQSEYGAD AVSGLHEDPP 550
    RMFSEEYQTA LLENYHLILD EKRKEYVIGE LIWNFADFMT NQSPLRVTGN 600
    KKGIFTRQRN PKMAAFILRE RYWRIANETR GYGSVPRTQC MGSRPFTF 648
    Length:648
    Mass (Da):74,793
    Last modified:January 1, 1988 - v1
    Checksum:i5ADE8F5234F0907E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141Q → E in CAA68705. (PubMed:3355537)Curated
    Sequence conflicti21 – 211V → L in CAA68705. (PubMed:3355537)Curated
    Sequence conflicti487 – 4871M → L in CAA68705. (PubMed:3355537)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13962 mRNA. Translation: AAA41228.1.
    Y00717 mRNA. Translation: CAA68705.1.
    PIRiA25047.
    RefSeqiNP_058711.2. NM_017015.2.
    UniGeneiRn.3692.

    Genome annotation databases

    GeneIDi24434.
    KEGGirno:24434.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13962 mRNA. Translation: AAA41228.1 .
    Y00717 mRNA. Translation: CAA68705.1 .
    PIRi A25047.
    RefSeqi NP_058711.2. NM_017015.2.
    UniGenei Rn.3692.

    3D structure databases

    ProteinModelPortali P06760.
    SMRi P06760. Positions 22-628.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P06760. 1 interaction.
    STRINGi 10116.ENSRNOP00000045033.

    Chemistry

    BindingDBi P06760.
    ChEMBLi CHEMBL4814.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    PTM databases

    PhosphoSitei P06760.

    Proteomic databases

    PaxDbi P06760.
    PRIDEi P06760.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24434.
    KEGGi rno:24434.

    Organism-specific databases

    CTDi 2990.
    RGDi 2772. Gusb.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000120896.
    HOVERGENi HBG004843.
    KOi K01195.

    Enzyme and pathway databases

    SABIO-RK P06760.

    Miscellaneous databases

    NextBioi 603309.

    Gene expression databases

    Genevestigatori P06760.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SUPFAMi SSF49303. SSF49303. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of rat preputial gland beta-glucuronidase cDNA and in vitro insertion of its encoded polypeptide into microsomal membranes."
      Nishimura Y., Rosenfeld M.G., Kreibich G., Gubler U., Sabatini D.D., Adesnik M., Andy R.
      Proc. Natl. Acad. Sci. U.S.A. 83:7292-7296(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Preputial gland.
    2. "Rat liver beta-glucuronidase. cDNA cloning, sequence comparisons and expression of a chimeric protein in COS cells."
      Powell P.P., Kyle J.W., Miller R.D., Pantano J., Grubb J.H., Sly W.S.
      Biochem. J. 250:547-555(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-648.
      Tissue: Liver.

    Entry informationi

    Entry nameiBGLR_RAT
    AccessioniPrimary (citable) accession number: P06760
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3