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Reviewed, UniProtKB/Swiss-Prot P06760 (BGLR_RAT)

Last modified June 16, 2009. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Beta-glucuronidase
    EC=3.2.1.31
Gene names
Name: Gusb
Synonyms: Gus
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length648 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Plays an important role in the degradation of dermatan and keratan sulfates.

Catalytic activity

A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

Enzyme regulation

Inhibited by L-aspartic acid By similarity.

Subunit structure

Homotetramer.

Subcellular location

Lysosome.

Post-translational modification

Undergoes a post-transcriptional proteolytic cleavage near its C-terminal end, which reduces its size by approximately 3 kDa. The site of this cleavage has as yet not been determined.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

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Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentlysosome

Inferred from direct assay. Source: RGD

soluble fraction

Inferred from direct assay. Source: RGD

   Molecular functionbeta-glucuronidase activity Ref.2

Inferred from direct assay. Source: RGD

cation binding

Inferred from electronic annotation. Source: InterPro

sugar binding

Inferred from direct assay. Source: RGD

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222
Chain23 – 648626Beta-glucuronidase
PRO_0000012163

Sites

Active site4471Proton donor By similarity

Amino acid modifications

Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation4161N-linked (GlcNAc...) Potential
Glycosylation5911N-linked (GlcNAc...) Potential
Glycosylation6271N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict141Q → E in CAA68705. Ref.2
Sequence conflict211V → L in CAA68705. Ref.2
Sequence conflict4871M → L in CAA68705. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
P06760-1 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 5ADE8F5234F0907E

FASTA64874,793
        10         20         30         40         50         60 
MSPRRSVCWF VLGQLLCSCA VALQGGMLFP KETPSRELKV LDGLWSFRAD YSNNRLQGFE 

        70         80         90        100        110        120 
KQWYRQPLRE SGPTLDMPVP SSFNDITQEA ELRNFIGWVW YEREAVLPQR WTQDTDRRVV 

       130        140        150        160        170        180 
LRINSAHYYA VVWVNGIHVV EHEGGHLPFE ADITKLVQSG PLTTFRVTIA INNTLTPYTL 

       190        200        210        220        230        240 
PPGTIVYKTD PSMYPKGYFV QDISFDFFNY AGLHRSVVLY TTPTTYIDDI TVTTDVDRDV 

       250        260        270        280        290        300 
GLVNYWISVQ GSDHFQLEVR LLDEDGKIVA RGTGNEGQLK VPRAHLWWPY LMHEHPAYLY 

       310        320        330        340        350        360 
SLEVTMTTPE SVSDFYTLPV GIRTVAVTKS KFLINGKPFY FQGVNKHEDS DIRGRGFDWP 

       370        380        390        400        410        420 
LLIKDFNLLR WLGANSFRTS HYPYSEEVLQ LCDRYGIVVI DECPGVGIVL PQSFGNVSLR 

       430        440        450        460        470        480 
HHLEVMDELV RRDKNHPAVV MWSVANEPVS SLKPAGYYFK TLIAHTKALD PTRPVTFVSN 

       490        500        510        520        530        540 
TRYDADMGAP YVDVICVNSY LSWYHDYGHL EVIQLQLTSQ FENWYKMYQK PIIQSEYGAD 

       550        560        570        580        590        600 
AVSGLHEDPP RMFSEEYQTA LLENYHLILD EKRKEYVIGE LIWNFADFMT NQSPLRVTGN 

       610        620        630        640 
KKGIFTRQRN PKMAAFILRE RYWRIANETR GYGSVPRTQC MGSRPFTF

« Hide

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References

[1]"Nucleotide sequence of rat preputial gland beta-glucuronidase cDNA and in vitro insertion of its encoded polypeptide into microsomal membranes."
Nishimura Y., Rosenfeld M.G., Kreibich G., Gubler U., Sabatini D.D., Adesnik M., Andy R.
Proc. Natl. Acad. Sci. U.S.A. 83:7292-7296(1986) [PubMed: 3463967] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Preputial gland.
[2]"Rat liver beta-glucuronidase. cDNA cloning, sequence comparisons and expression of a chimeric protein in COS cells."
Powell P.P., Kyle J.W., Miller R.D., Pantano J., Grubb J.H., Sly W.S.
Biochem. J. 250:547-555(1988) [PubMed: 3355537] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-648.
Tissue: Liver.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M13962 mRNA. Translation: AAA41228.1.
Y00717 mRNA. Translation: CAA68705.1.
IPIIPI00206623.
PIRA25047.
RefSeqNP_058711.2.
UniGeneRn.3692

3D structure databases

HSSPHSSP built from PDB template 1BHG based on UniProtKB P08236.
SMRP06760. Positions 22-628.
ModBaseSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEP06760.

Genome annotation databases

EnsemblENSRNOG00000000913. Rattus norvegicus. [Contig view]
GeneID24434.
KEGGrno:24434.

Organism-specific databases

RGD2772. Gusb.

Phylogenomic databases

HOVERGENP06760.

Enzyme and pathway databases

BRENDA3.2.1.31. 248.

Family and domain databases

InterProIPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006104. Glyco_hydro_2_carb-bd.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio603309.

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Entry information

Entry nameBGLR_RAT
AccessionPrimary (citable) accession number: P06760
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 16, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents