Reviewed, UniProtKB/Swiss-Prot P06758 (ADH2_ZYMMO)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase 2
    EC=1.1.1.1
Alternative name(s):
    Alcohol dehydrogenase II
      Short name=ADH II

Gene names

Name:	adhB
Ordered Locus Names:	ZMO1596

Organism

Zymomonas mobilis [Complete proteome] [HAMAP]

Taxonomic identifier

542 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Iron.
Subunit structure	Homotetramer.
Miscellaneous	In Z.mobilis there are two isozymes of alcohol dehydrogenase.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	Iron NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 383	382	Alcohol dehydrogenase 2		PRO_0000087815
Experimental info
	Sequence conflict	38	1	S → G AA sequence Ref.4
	Sequence conflict	45 – 48	4	SGVV → GVS AA sequence Ref.4
	Sequence conflict	57	1	A → T in CAA34911. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P06758-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E1C3A159B1652342	FASTA	383	40,145
	10 20 30 40 50 60 MASSTFYIPF VNEMGEGSLE KAIKDLNGSG FKNALIVSDA FMNKSGVVKQ VADLLKAQGI 70 80 90 100 110 120 NSAVYDGVMP NPTVTAVLEG LKILKDNNSD FVISLGGGSP HDCAKAIALV ATNGGEVKDY 130 140 150 160 170 180 EGIDKSKKPA LPLMSINTTA GTASEMTRFC IITDEVRHVK MAIVDRHVTP MVSVNDPLLM 190 200 210 220 230 240 VGMPKGLTAA TGMDALTHAF EAYSSTAATP ITDACALKAA SMIAKNLKTA CDNGKDMPAR 250 260 270 280 290 300 EAMAYAQFLA GMAFNNASLG YVHAMAHQLG GYYNLPHGVC NAVLLPHVLA YNASVVAGRL 310 320 330 340 350 360 KDVGVAMGLD IANLGDKEGA EATIQAVRDL AASIGIPANL TELGAKKEDV PLLADHALKD 370 380 ACALTNPRQG DQKEVEELFL SAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis." Conway T., Sewell G.W., Osman Y.A., Ingram L.O. J. Bacteriol. 169:2591-2597(1987) [PubMed: 3584063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the Zymomonas mobilis alcohol dehydrogenase II gene." Yoon K.-H., Pack M.Y. Nucleic Acids Res. 18:187-187(1990) [PubMed: 2308827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10988 / NCIB 8938 / NRRL B-806 / ZM1.
[3]	"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.
[4]	"The two alcohol dehydrogenases of Zymomonas mobilis. Purification by differential dye ligand chromatography, molecular characterisation and physiological roles." Neale A.D., Scopes R.K., Kelly J.M., Wettenhall R.E.H. Eur. J. Biochem. 154:119-124(1986) [PubMed: 2935393] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-50.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M15394 Genomic DNA. Translation: AAA27683.1. X17065 Genomic DNA. Translation: CAA34911.1. AE008692 Genomic DNA. Translation: AAV90220.1.
PIR	A25978.
RefSeq	YP_163331.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3187659.
GenomeReviews	Gene locus ZMO1596 in contig AE008692_GR.
KEGG	zmo:ZMO1596.
NMPDR	fig|264203.3.peg.70.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P06758.
OMA	ARDQMAY.
Enzyme and pathway databases
BioCyc	ZMOB264203:ZMO1596-MON.
BRENDA	1.1.1.1. 1658.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADH2_ZYMMO

Accession

Primary (citable) accession number: P06758
Secondary accession number(s): Q5NM40

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)