Alcohol dehydrogenase 1 - Rattus norvegicus (Rat)

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Reviewed, UniProtKB/Swiss-Prot P06757 (ADH1_RAT)

Last modified June 16, 2009. Version 85. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase 1
    EC=1.1.1.1
Alternative name(s):
    Alcohol dehydrogenase A subunit

Gene names

Name:	Adh1
Synonyms:	Adh-1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	An alcohol + NAD⁺ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit.
Subunit structure	Dimer of identical or non-identical chains of three types (A, B, C), which are coded by 3 separate genes at different loci.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-I subfamily.

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Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	acetaldehyde biosynthetic process Inferred from direct assay. Source: RGD ethanol oxidation Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	NAD or NADH binding Inferred from direct assay. Source: RGD alcohol dehydrogenase activity Inferred from direct assay. Source: RGD drug binding Inferred from physical interaction. Source: RGD ethanol binding Inferred from direct assay. Source: RGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 376

375

Alcohol dehydrogenase 1

PRO_0000160669

Regions

Nucleotide binding

201 – 206

NAD By similarity

Nucleotide binding

294 – 296

NAD By similarity

Sites

Metal binding

Zinc 1; catalytic

Metal binding

Zinc 1; catalytic

Metal binding

Zinc 2

Metal binding

101

Zinc 2

Metal binding

104

Zinc 2

Metal binding

112

Zinc 2

Metal binding

176

Zinc 1; catalytic

Binding site

225

NAD By similarity

Binding site

230

NAD By similarity

Binding site

371

NAD By similarity

Amino acid modifications

Modified residue

N-acetylserine Ref.4

Experimental info

Sequence conflict

143

I → L in AAA40681. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P06757-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 3C7026C6D7C0ED76
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FASTA

376

39,645

        10         20         30         40         50         60 
MSTAGKVIKC KAAVLWEPHK PFTIEDIEVA PPKAHEVRIK MVATGVCRSD DHAVSGSLFT 

        70         80         90        100        110        120 
PLPAVLGHEG AGIVESIGEG VTCVKPGDKV IPLFSPQCGK CRICKHPESN LCCQTKNLTQ 

       130        140        150        160        170        180 
PKGALLDGTS RFSCRGKPIH HFISTSTFSQ YTVVDDIAVA KIDAAAPLDK VCLIGCGFST 

       190        200        210        220        230        240 
GYGSAVQVAK VTPGSTCAVF GLGGVGLSVV IGCKTAGAAK IIAVDINKDK FAKAKELGAT 

       250        260        270        280        290        300 
DCINPQDYTK PIQEVLQEMT DGGVDFSFEV IGRLDTMTSA LLSCHSACGV SVIVGVPPSA 

       310        320        330        340        350        360 
QSLSVNPMSL LLGRTWKGAI FGGFKSKDAV PKLVADFMAK KFPLEPLITH VLPFEKINEA 

       370 
FDLLRAGKSI RTVLTF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete amino acid sequence of rat liver alcohol dehydrogenase deduced from the cDNA sequence." Crabb D.W., Edenberg H.J. Gene 48:287-291(1986) [PubMed: 2881847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Structure and expression of the rat class I alcohol dehydrogenase gene." Crabb D.W., Stein P.M., Dipple K.M., Hittle J.B., Sidhu R., Qulali M., Zhang K., Edenberg H.J. Genomics 5:906-914(1989) [PubMed: 2591969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[4]	"Structural studies of alcohol dehydrogenase from rat liver." Joernvall H., Markovic O. Eur. J. Biochem. 29:167-174(1972) [PubMed: 4673366] [Abstract] Cited for: ACETYLATION AT SER-2.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	M29523 M29522 Genomic DNA. Translation: AAA85462.1. M15327 mRNA. Translation: AAA40681.1. BC062403 mRNA. Translation: AAH62403.1.
IPI	IPI00331983.
PIR	A26468.
RefSeq	NP_062159.3.
UniGene	Rn.40222
3D structure databases
HSSP	HSSP built from PDB template 1HT0 based on UniProtKB P00326.
SMR	P06757. Positions 2-376.
ModBase	Search...
Proteomic databases
PRIDE	P06757.
Genome annotation databases
Ensembl	ENSRNOG00000012464. Rattus norvegicus. [Contig view]
GeneID	24172.
KEGG	rno:24172.
Organism-specific databases
RGD	2044. Adh1.
Phylogenomic databases
HOVERGEN	P06757.
Enzyme and pathway databases
BRENDA	1.1.1.1. 248.
Gene expression databases
ArrayExpress	P06757.
GermOnline	ENSRNOG00000012464. Rattus norvegicus.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	602493.

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Entry information

Entry name

ADH1_RAT

Accession

Primary (citable) accession number: P06757

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents