UniProtKB - P06745 (G6PI_MOUSE)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Glucose-6-phosphate isomerase
Gene
Gpi
Organism
Mus musculus (Mouse)
Status
Functioni
Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Catalytic activityi
D-glucose 6-phosphate = D-fructose 6-phosphate.
: glycolysis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Glucose-6-phosphate isomerase (Gpi), Glucose-6-phosphate isomerase (Gpi1), Glucose-6-phosphate isomerase (Gpi1), Glucose-6-phosphate isomerase (Gpi1)
- ATP-dependent 6-phosphofructokinase, liver type (Pfkl), ATP-dependent 6-phosphofructokinase (Pfkp), ATP-dependent 6-phosphofructokinase (Pfkm), ATP-dependent 6-phosphofructokinase, platelet type (Pfkp), ATP-dependent 6-phosphofructokinase, muscle type (Pfkm)
- Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoart1), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoart2), Fructose-bisphosphate aldolase (Aldoart1), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldob), Fructose-bisphosphate aldolase C (Aldoc), Fructose-bisphosphate aldolase A (Aldoa), Fructose-bisphosphate aldolase B (Aldob), Fructose-bisphosphate aldolase (Aldob)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 358 | Proton donor1 Publication | 1 | |
Active sitei | 389 | 1 Publication | 1 | |
Active sitei | 519 | 1 Publication | 1 |
GO - Molecular functioni
- cytokine activity Source: UniProtKB-KW
- glucose-6-phosphate isomerase activity Source: MGI
- growth factor activity Source: UniProtKB-KW
- intramolecular transferase activity Source: Ensembl
- monosaccharide binding Source: Ensembl
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- aldehyde catabolic process Source: Ensembl
- angiogenesis Source: UniProtKB-KW
- erythrocyte homeostasis Source: MGI
- gluconeogenesis Source: UniProtKB-KW
- glucose 6-phosphate metabolic process Source: Ensembl
- glucose homeostasis Source: MGI
- glycolytic process Source: MGI
- glycolytic process through glucose-6-phosphate Source: MGI
- in utero embryonic development Source: MGI
- learning or memory Source: Ensembl
- mesoderm formation Source: MGI
- methylglyoxal biosynthetic process Source: Ensembl
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
- negative regulation of neuron apoptotic process Source: Ensembl
- positive regulation of immunoglobulin secretion Source: MGI
- response to cadmium ion Source: Ensembl
- response to estradiol Source: Ensembl
- response to immobilization stress Source: Ensembl
- response to morphine Source: Ensembl
- response to muscle stretch Source: Ensembl
- response to progesterone Source: Ensembl
- response to testosterone Source: Ensembl
Keywordsi
Molecular function | Cytokine, Growth factor, Isomerase |
Biological process | Gluconeogenesis, Glycolysis |
Enzyme and pathway databases
BRENDAi | 5.3.1.9 3474 |
Reactomei | R-MMU-5628897 TP53 Regulates Metabolic Genes R-MMU-6798695 Neutrophil degranulation R-MMU-70171 Glycolysis R-MMU-70263 Gluconeogenesis |
SABIO-RKi | P06745 |
UniPathwayi | UPA00109; UER00181 |
Protein family/group databases
MoonProti | P06745 |
Names & Taxonomyi
Protein namesi | Recommended name: Glucose-6-phosphate isomerase (EC:5.3.1.9)Short name: GPI Alternative name(s): Autocrine motility factor Short name: AMF Neuroleukin Short name: NLK Phosphoglucose isomerase Short name: PGI Phosphohexose isomerase Short name: PHI |
Gene namesi | Name:Gpi Synonyms:Gpi1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95797 Gpi1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000180538 | 2 – 558 | Glucose-6-phosphate isomeraseAdd BLAST | 557 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 12 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 86 | PhosphoserineBy similarity | 1 | |
Modified residuei | 107 | PhosphoserineBy similarity | 1 | |
Modified residuei | 109 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 142 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 185 | Phosphoserine; by CK2By similarity | 1 | |
Modified residuei | 250 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 454 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 454 | N6-malonyllysine; alternateBy similarity | 1 | |
Modified residuei | 454 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 455 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
ISGylated.1 Publication
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P06745 |
MaxQBi | P06745 |
PaxDbi | P06745 |
PeptideAtlasi | P06745 |
PRIDEi | P06745 |
PTM databases
iPTMneti | P06745 |
PhosphoSitePlusi | P06745 |
SwissPalmi | P06745 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000036427 |
CleanExi | MM_GPI1 |
ExpressionAtlasi | P06745 baseline and differential |
Genevisiblei | P06745 MM |
Interactioni
Subunit structurei
Homodimer in the catalytically active form, monomer in the secreted form.2 Publications
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
Parp14 | Q2EMV9 | 4 | EBI-1534927,EBI-1534943 |
GO - Molecular functioni
- cytokine activity Source: UniProtKB-KW
- growth factor activity Source: UniProtKB-KW
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGridi | 2000215 interactors. |
IntActi | P06745 7 interactors. |
MINTi | P06745 |
STRINGi | 10090.ENSMUSP00000049355 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 3 – 6 | Combined sources | 4 | |
Helixi | 8 – 20 | Combined sources | 13 | |
Helixi | 21 – 23 | Combined sources | 3 | |
Helixi | 26 – 32 | Combined sources | 7 | |
Helixi | 36 – 39 | Combined sources | 4 | |
Beta strandi | 41 – 45 | Combined sources | 5 | |
Beta strandi | 50 – 54 | Combined sources | 5 | |
Helixi | 62 – 74 | Combined sources | 13 | |
Helixi | 77 – 85 | Combined sources | 9 | |
Turni | 92 – 95 | Combined sources | 4 | |
Helixi | 100 – 103 | Combined sources | 4 | |
Beta strandi | 116 – 118 | Combined sources | 3 | |
Helixi | 119 – 137 | Combined sources | 19 | |
Beta strandi | 151 – 155 | Combined sources | 5 | |
Helixi | 158 – 160 | Combined sources | 3 | |
Helixi | 162 – 170 | Combined sources | 9 | |
Helixi | 172 – 175 | Combined sources | 4 | |
Beta strandi | 180 – 184 | Combined sources | 5 | |
Helixi | 189 – 196 | Combined sources | 8 | |
Turni | 201 – 203 | Combined sources | 3 | |
Beta strandi | 204 – 209 | Combined sources | 6 | |
Beta strandi | 211 – 213 | Combined sources | 3 | |
Helixi | 216 – 233 | Combined sources | 18 | |
Helixi | 236 – 241 | Combined sources | 6 | |
Beta strandi | 243 – 248 | Combined sources | 6 | |
Helixi | 250 – 256 | Combined sources | 7 | |
Helixi | 260 – 262 | Combined sources | 3 | |
Beta strandi | 263 – 265 | Combined sources | 3 | |
Helixi | 272 – 274 | Combined sources | 3 | |
Turni | 276 – 278 | Combined sources | 3 | |
Helixi | 279 – 281 | Combined sources | 3 | |
Helixi | 282 – 288 | Combined sources | 7 | |
Helixi | 290 – 309 | Combined sources | 20 | |
Helixi | 312 – 314 | Combined sources | 3 | |
Helixi | 316 – 329 | Combined sources | 14 | |
Beta strandi | 335 – 341 | Combined sources | 7 | |
Helixi | 343 – 345 | Combined sources | 3 | |
Helixi | 348 – 360 | Combined sources | 13 | |
Beta strandi | 378 – 380 | Combined sources | 3 | |
Turni | 384 – 387 | Combined sources | 4 | |
Helixi | 388 – 397 | Combined sources | 10 | |
Beta strandi | 404 – 411 | Combined sources | 8 | |
Helixi | 417 – 419 | Combined sources | 3 | |
Helixi | 420 – 438 | Combined sources | 19 | |
Helixi | 442 – 451 | Combined sources | 10 | |
Helixi | 456 – 462 | Combined sources | 7 | |
Helixi | 463 – 466 | Combined sources | 4 | |
Beta strandi | 474 – 481 | Combined sources | 8 | |
Helixi | 484 – 505 | Combined sources | 22 | |
Helixi | 513 – 515 | Combined sources | 3 | |
Helixi | 516 – 525 | Combined sources | 10 | |
Helixi | 526 – 529 | Combined sources | 4 | |
Beta strandi | 530 – 533 | Combined sources | 4 | |
Helixi | 540 – 552 | Combined sources | 13 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1U0E | X-ray | 1.60 | A/B | 1-558 | [»] | |
1U0F | X-ray | 1.60 | A/B | 1-558 | [»] | |
1U0G | X-ray | 1.70 | A/B | 1-558 | [»] | |
2CVP | X-ray | 1.80 | A/B | 1-557 | [»] | |
2CXN | X-ray | 1.40 | A/B | 1-557 | [»] | |
2CXO | X-ray | 1.80 | A/B | 1-557 | [»] | |
2CXP | X-ray | 1.70 | A/B | 1-557 | [»] | |
2CXQ | X-ray | 1.50 | A/B | 1-557 | [»] | |
2CXR | X-ray | 1.70 | A/B | 1-557 | [»] | |
2CXS | X-ray | 1.50 | A/B | 1-557 | [»] | |
2CXT | X-ray | 1.50 | A/B | 1-557 | [»] | |
2CXU | X-ray | 1.65 | A/B | 1-557 | [»] | |
ProteinModelPortali | P06745 | |||||
SMRi | P06745 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06745 |
Family & Domainsi
Sequence similaritiesi
Belongs to the GPI family.Curated
Phylogenomic databases
eggNOGi | KOG2446 Eukaryota COG0166 LUCA |
GeneTreei | ENSGT00390000000707 |
HOVERGENi | HBG002877 |
InParanoidi | P06745 |
KOi | K01810 |
OMAi | TNSQHAF |
OrthoDBi | EOG091G03ZI |
PhylomeDBi | P06745 |
TreeFami | TF300436 |
Family and domain databases
CDDi | cd05015 SIS_PGI_1, 1 hit cd05016 SIS_PGI_2, 1 hit |
Gene3Di | 1.10.1390.101 hit |
HAMAPi | MF_00473 G6P_isomerase, 1 hit |
InterProi | View protein in InterPro IPR001672 G6P_Isomerase IPR023096 G6P_Isomerase_C IPR018189 Phosphoglucose_isomerase_CS IPR035476 SIS_PGI_1 IPR035482 SIS_PGI_2 |
PANTHERi | PTHR11469 PTHR11469, 1 hit |
Pfami | View protein in Pfam PF00342 PGI, 1 hit |
PRINTSi | PR00662 G6PISOMERASE |
PROSITEi | View protein in PROSITE PS00765 P_GLUCOSE_ISOMERASE_1, 1 hit PS00174 P_GLUCOSE_ISOMERASE_2, 1 hit PS51463 P_GLUCOSE_ISOMERASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P06745-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAALTRNPQF QKLLEWHRAN SANLKLRELF EADPERFNNF SLNLNTNHGH
60 70 80 90 100
ILVDYSKNLV NKEVMQMLVE LAKSRGVEAA RDNMFSGSKI NYTENRAVLH
110 120 130 140 150
VALRNRSNTP IKVDGKDVMP EVNRVLDKMK SFCQRVRSGD WKGYTGKSIT
160 170 180 190 200
DIINIGIGGS DLGPLMVTEA LKPYSKGGPR VWFVSNIDGT HIAKTLASLS
210 220 230 240 250
PETSLFIIAS KTFTTQETIT NAETAKEWFL EAAKDPSAVA KHFVALSTNT
260 270 280 290 300
AKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DHFEQLLSGA
310 320 330 340 350
HWMDQHFLKT PLEKNAPVLL ALLGIWYINC YGCETHALLP YDQYMHRFAA
360 370 380 390 400
YFQQGDMESN GKYITKSGAR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK
410 420 430 440 450
MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMKGK LPEEARKELQ
460 470 480 490 500
AAGKSPEDLE KLLPHKVFEG NRPTNSIVFT KLTPFILGAL IAMYEHKIFV
510 520 530 540 550
QGIMWDINSF DQWGVELGKQ LAKKIEPELE GSSAVTSHDS STNGLISFIK
QQRDTKLE
Sequence cautioni
The sequence BAC36335 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 95 | N → D in AAA39825 (PubMed:3764429).Curated | 1 | |
Sequence conflicti | 95 | N → D in BAE41301 (PubMed:3764429).Curated | 1 | |
Sequence conflicti | 119 | M → K in BAE21866 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 191 | H → R in BAE22329 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 238 | A → E in AAC36515 (PubMed:9798653).Curated | 1 | |
Sequence conflicti | 264 – 266 | FEF → LEL in AAC36515 (PubMed:9798653).Curated | 3 | |
Sequence conflicti | 285 | A → V in AAC36515 (PubMed:9798653).Curated | 1 | |
Sequence conflicti | 303 | M → T in AAC36515 (PubMed:9798653).Curated | 1 | |
Sequence conflicti | 313 | E → G in BAE35416 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 357 | M → V in AAC36515 (PubMed:9798653).Curated | 1 | |
Sequence conflicti | 372 | D → N in AAC36515 (PubMed:9798653).Curated | 1 | |
Sequence conflicti | 380 | W → L in AAC36515 (PubMed:9798653).Curated | 1 | |
Sequence conflicti | 499 | F → L in BAE23502 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14220 mRNA Translation: AAA39825.1 AK076424 mRNA Translation: BAC36335.1 Different initiation. AK133827 mRNA Translation: BAE21866.1 AK134890 mRNA Translation: BAE22329.1 AK137805 mRNA Translation: BAE23502.1 AK147124 mRNA Translation: BAE27695.1 AK150341 mRNA Translation: BAE29481.1 AK159838 mRNA Translation: BAE35416.1 AK169681 mRNA Translation: BAE41301.1 BX537302 Genomic DNA No translation available. BC086640 mRNA Translation: AAH86640.1 BC088995 mRNA Translation: AAH88995.1 U89408 mRNA Translation: AAC36515.1 L09104 mRNA Translation: AAA65641.1 AF108354 mRNA Translation: AAF28799.1 |
CCDSi | CCDS21138.1 |
PIRi | A24439 NUMS |
RefSeqi | NP_032181.2, NM_008155.4 |
UniGenei | Mm.589 |
Genome annotation databases
Ensembli | ENSMUST00000038027; ENSMUSP00000049355; ENSMUSG00000036427 |
GeneIDi | 14751 |
KEGGi | mmu:14751 |
UCSCi | uc009gix.3 mouse |
Similar proteinsi
Entry informationi
Entry namei | G6PI_MOUSE | |
Accessioni | P06745Primary (citable) accession number: P06745 Secondary accession number(s): O89062 Q9JM07 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | May 1, 2007 | |
Last modified: | April 25, 2018 | |
This is version 162 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |