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UniProtKB - P06745 (G6PI_MOUSE)

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Protein

Glucose-6-phosphate isomerase

Gene

Gpi

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Gpi), Glucose-6-phosphate isomerase (Gpi1), Glucose-6-phosphate isomerase (Gpi1), Glucose-6-phosphate isomerase (Gpi1)
  3. ATP-dependent 6-phosphofructokinase, liver type (Pfkl), ATP-dependent 6-phosphofructokinase (Pfkp), ATP-dependent 6-phosphofructokinase (Pfkm), ATP-dependent 6-phosphofructokinase, platelet type (Pfkp), ATP-dependent 6-phosphofructokinase, muscle type (Pfkm)
  4. Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoart1), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoart2), Fructose-bisphosphate aldolase (Aldoart1), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldob), Fructose-bisphosphate aldolase C (Aldoc), Fructose-bisphosphate aldolase A (Aldoa), Fructose-bisphosphate aldolase B (Aldob), Fructose-bisphosphate aldolase (Aldob)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei358Proton donor1 Publication1
Active sitei3891 Publication1
Active sitei5191 Publication1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • aldehyde catabolic process Source: Ensembl
  • angiogenesis Source: UniProtKB-KW
  • erythrocyte homeostasis Source: MGI
  • gluconeogenesis Source: UniProtKB-KW
  • glucose 6-phosphate metabolic process Source: Ensembl
  • glucose homeostasis Source: MGI
  • glycolytic process Source: MGI
  • glycolytic process through glucose-6-phosphate Source: MGI
  • in utero embryonic development Source: MGI
  • learning or memory Source: Ensembl
  • mesoderm formation Source: MGI
  • methylglyoxal biosynthetic process Source: Ensembl
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  • negative regulation of neuron apoptotic process Source: Ensembl
  • positive regulation of immunoglobulin secretion Source: MGI
  • response to cadmium ion Source: Ensembl
  • response to estradiol Source: Ensembl
  • response to immobilization stress Source: Ensembl
  • response to morphine Source: Ensembl
  • response to muscle stretch Source: Ensembl
  • response to progesterone Source: Ensembl
  • response to testosterone Source: Ensembl
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionCytokine, Growth factor, Isomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

BRENDAi5.3.1.9 3474
ReactomeiR-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-6798695 Neutrophil degranulation
R-MMU-70171 Glycolysis
R-MMU-70263 Gluconeogenesis
SABIO-RKiP06745
UniPathwayiUPA00109; UER00181

Protein family/group databases

MoonProtiP06745

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Autocrine motility factor
Short name:
AMF
Neuroleukin
Short name:
NLK
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:Gpi
Synonyms:Gpi1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:95797 Gpi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001805382 – 558Glucose-6-phosphate isomeraseAdd BLAST557

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei12N6-acetyllysineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Modified residuei107PhosphoserineBy similarity1
Modified residuei109PhosphothreonineBy similarity1
Modified residuei142N6-acetyllysineCombined sources1
Modified residuei185Phosphoserine; by CK2By similarity1
Modified residuei250PhosphothreonineBy similarity1
Modified residuei454N6-acetyllysine; alternateCombined sources1
Modified residuei454N6-malonyllysine; alternateBy similarity1
Modified residuei454N6-succinyllysine; alternateCombined sources1
Modified residuei455PhosphoserineCombined sources1

Post-translational modificationi

ISGylated.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP06745
MaxQBiP06745
PaxDbiP06745
PeptideAtlasiP06745
PRIDEiP06745

PTM databases

iPTMnetiP06745
PhosphoSitePlusiP06745
SwissPalmiP06745

Expressioni

Gene expression databases

BgeeiENSMUSG00000036427
CleanExiMM_GPI1
ExpressionAtlasiP06745 baseline and differential
GenevisibleiP06745 MM

Interactioni

Subunit structurei

Homodimer in the catalytically active form, monomer in the secreted form.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Parp14Q2EMV94EBI-1534927,EBI-1534943

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi2000215 interactors.
IntActiP06745 7 interactors.
MINTiP06745
STRINGi10090.ENSMUSP00000049355

Structurei

Secondary structure

1558
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 6Combined sources4
Helixi8 – 20Combined sources13
Helixi21 – 23Combined sources3
Helixi26 – 32Combined sources7
Helixi36 – 39Combined sources4
Beta strandi41 – 45Combined sources5
Beta strandi50 – 54Combined sources5
Helixi62 – 74Combined sources13
Helixi77 – 85Combined sources9
Turni92 – 95Combined sources4
Helixi100 – 103Combined sources4
Beta strandi116 – 118Combined sources3
Helixi119 – 137Combined sources19
Beta strandi151 – 155Combined sources5
Helixi158 – 160Combined sources3
Helixi162 – 170Combined sources9
Helixi172 – 175Combined sources4
Beta strandi180 – 184Combined sources5
Helixi189 – 196Combined sources8
Turni201 – 203Combined sources3
Beta strandi204 – 209Combined sources6
Beta strandi211 – 213Combined sources3
Helixi216 – 233Combined sources18
Helixi236 – 241Combined sources6
Beta strandi243 – 248Combined sources6
Helixi250 – 256Combined sources7
Helixi260 – 262Combined sources3
Beta strandi263 – 265Combined sources3
Helixi272 – 274Combined sources3
Turni276 – 278Combined sources3
Helixi279 – 281Combined sources3
Helixi282 – 288Combined sources7
Helixi290 – 309Combined sources20
Helixi312 – 314Combined sources3
Helixi316 – 329Combined sources14
Beta strandi335 – 341Combined sources7
Helixi343 – 345Combined sources3
Helixi348 – 360Combined sources13
Beta strandi378 – 380Combined sources3
Turni384 – 387Combined sources4
Helixi388 – 397Combined sources10
Beta strandi404 – 411Combined sources8
Helixi417 – 419Combined sources3
Helixi420 – 438Combined sources19
Helixi442 – 451Combined sources10
Helixi456 – 462Combined sources7
Helixi463 – 466Combined sources4
Beta strandi474 – 481Combined sources8
Helixi484 – 505Combined sources22
Helixi513 – 515Combined sources3
Helixi516 – 525Combined sources10
Helixi526 – 529Combined sources4
Beta strandi530 – 533Combined sources4
Helixi540 – 552Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U0EX-ray1.60A/B1-558[»]
1U0FX-ray1.60A/B1-558[»]
1U0GX-ray1.70A/B1-558[»]
2CVPX-ray1.80A/B1-557[»]
2CXNX-ray1.40A/B1-557[»]
2CXOX-ray1.80A/B1-557[»]
2CXPX-ray1.70A/B1-557[»]
2CXQX-ray1.50A/B1-557[»]
2CXRX-ray1.70A/B1-557[»]
2CXSX-ray1.50A/B1-557[»]
2CXTX-ray1.50A/B1-557[»]
2CXUX-ray1.65A/B1-557[»]
ProteinModelPortaliP06745
SMRiP06745
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06745

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Phylogenomic databases

eggNOGiKOG2446 Eukaryota
COG0166 LUCA
GeneTreeiENSGT00390000000707
HOVERGENiHBG002877
InParanoidiP06745
KOiK01810
OMAiTNSQHAF
OrthoDBiEOG091G03ZI
PhylomeDBiP06745
TreeFamiTF300436

Family and domain databases

CDDicd05015 SIS_PGI_1, 1 hit
cd05016 SIS_PGI_2, 1 hit
Gene3Di1.10.1390.101 hit
HAMAPiMF_00473 G6P_isomerase, 1 hit
InterProiView protein in InterPro
IPR001672 G6P_Isomerase
IPR023096 G6P_Isomerase_C
IPR018189 Phosphoglucose_isomerase_CS
IPR035476 SIS_PGI_1
IPR035482 SIS_PGI_2
PANTHERiPTHR11469 PTHR11469, 1 hit
PfamiView protein in Pfam
PF00342 PGI, 1 hit
PRINTSiPR00662 G6PISOMERASE
PROSITEiView protein in PROSITE
PS00765 P_GLUCOSE_ISOMERASE_1, 1 hit
PS00174 P_GLUCOSE_ISOMERASE_2, 1 hit
PS51463 P_GLUCOSE_ISOMERASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALTRNPQF QKLLEWHRAN SANLKLRELF EADPERFNNF SLNLNTNHGH 
        60         70         80         90        100
ILVDYSKNLV NKEVMQMLVE LAKSRGVEAA RDNMFSGSKI NYTENRAVLH 
       110        120        130        140        150
VALRNRSNTP IKVDGKDVMP EVNRVLDKMK SFCQRVRSGD WKGYTGKSIT 
       160        170        180        190        200
DIINIGIGGS DLGPLMVTEA LKPYSKGGPR VWFVSNIDGT HIAKTLASLS 
       210        220        230        240        250
PETSLFIIAS KTFTTQETIT NAETAKEWFL EAAKDPSAVA KHFVALSTNT 
       260        270        280        290        300
AKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DHFEQLLSGA 
       310        320        330        340        350
HWMDQHFLKT PLEKNAPVLL ALLGIWYINC YGCETHALLP YDQYMHRFAA 
       360        370        380        390        400
YFQQGDMESN GKYITKSGAR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK 
       410        420        430        440        450
MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMKGK LPEEARKELQ 
       460        470        480        490        500
AAGKSPEDLE KLLPHKVFEG NRPTNSIVFT KLTPFILGAL IAMYEHKIFV 
       510        520        530        540        550
QGIMWDINSF DQWGVELGKQ LAKKIEPELE GSSAVTSHDS STNGLISFIK 

QQRDTKLE
Length:558
Mass (Da):62,767
Last modified:May 1, 2007 - v4
Checksum:i7299E98B12B4C375
GO

Sequence cautioni

The sequence BAC36335 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95N → D in AAA39825 (PubMed:3764429).Curated1
Sequence conflicti95N → D in BAE41301 (PubMed:3764429).Curated1
Sequence conflicti119M → K in BAE21866 (PubMed:16141072).Curated1
Sequence conflicti191H → R in BAE22329 (PubMed:16141072).Curated1
Sequence conflicti238A → E in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti264 – 266FEF → LEL in AAC36515 (PubMed:9798653).Curated3
Sequence conflicti285A → V in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti303M → T in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti313E → G in BAE35416 (PubMed:16141072).Curated1
Sequence conflicti357M → V in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti372D → N in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti380W → L in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti499F → L in BAE23502 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14220 mRNA Translation: AAA39825.1
AK076424 mRNA Translation: BAC36335.1 Different initiation.
AK133827 mRNA Translation: BAE21866.1
AK134890 mRNA Translation: BAE22329.1
AK137805 mRNA Translation: BAE23502.1
AK147124 mRNA Translation: BAE27695.1
AK150341 mRNA Translation: BAE29481.1
AK159838 mRNA Translation: BAE35416.1
AK169681 mRNA Translation: BAE41301.1
BX537302 Genomic DNA No translation available.
BC086640 mRNA Translation: AAH86640.1
BC088995 mRNA Translation: AAH88995.1
U89408 mRNA Translation: AAC36515.1
L09104 mRNA Translation: AAA65641.1
AF108354 mRNA Translation: AAF28799.1
CCDSiCCDS21138.1
PIRiA24439 NUMS
RefSeqiNP_032181.2, NM_008155.4
UniGeneiMm.589

Genome annotation databases

EnsembliENSMUST00000038027; ENSMUSP00000049355; ENSMUSG00000036427
GeneIDi14751
KEGGimmu:14751
UCSCiuc009gix.3 mouse

Similar proteinsi

Entry informationi

Entry nameiG6PI_MOUSE
AccessioniPrimary (citable) accession number: P06745
Secondary accession number(s): O89062
, Q3TEE7, Q3TW50, Q3UUX1, Q3UY84, Q3UZJ1, Q5RJI3, Q8C675, Q9JM07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: May 1, 2007
Last modified: April 25, 2018
This is version 162 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome