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Protein

Myosin light chain alkali

Gene

Mlc1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • microfilament motor activity Source: UniProtKB

GO - Biological processi

  • mesoderm development Source: FlyBase
  • muscle contraction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Enzyme and pathway databases

ReactomeiR-DME-445355. Smooth Muscle Contraction.
R-DME-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain alkali
Gene namesi
Name:Mlc1
Synonyms:MLC-ALK
ORF Names:CG5596
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0002772. Mlc1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: FlyBase
  • muscle myosin complex Source: UniProtKB
  • myosin complex Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 155155Myosin light chain alkaliPRO_0000198709Add
BLAST

Proteomic databases

PaxDbiP06742.

Expressioni

Tissue specificityi

Indirect flight muscle isoform is found only in the indirect flight muscles. The larval and adult isoform is present in the larval and adult musculature.2 Publications

Developmental stagei

Expressed during late embryogenesis, larval instars, late stages of pupariation and adult.2 Publications

Gene expression databases

BgeeiP06742.
ExpressionAtlasiP06742. differential.
GenevisibleiP06742. DM.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Protein-protein interaction databases

BioGridi68205. 46 interactions.
DIPiDIP-23903N.
IntActiP06742. 20 interactions.
MINTiMINT-782616.
STRINGi7227.FBpp0084565.

Structurei

Secondary structure

1
155
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1914Combined sources
Turni22 – 243Combined sources
Beta strandi25 – 273Combined sources
Helixi28 – 303Combined sources
Helixi31 – 377Combined sources
Helixi44 – 496Combined sources
Helixi63 – 7412Combined sources
Helixi82 – 898Combined sources
Helixi90 – 923Combined sources
Beta strandi96 – 1016Combined sources
Helixi102 – 11110Combined sources
Beta strandi112 – 1143Combined sources
Helixi118 – 12811Combined sources
Beta strandi137 – 1393Combined sources
Helixi140 – 1478Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QBDX-ray2.23B/D1-151[»]
ProteinModelPortaliP06742.
SMRiP06742. Positions 1-151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 4135EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini80 – 11536EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ISFW. Eukaryota.
ENOG4111WV2. LUCA.
GeneTreeiENSGT00590000082921.
InParanoidiP06742.
OMAiMDEFLPI.
OrthoDBiEOG73NG53.
PhylomeDBiP06742.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Larval-adult (identifier: P06742-1) [UniParc]FASTAAdd to basket

Also known as: Larval-non-IFM

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADVPKREVE NVEFVFEVMG SPGEGIDAVD LGDALRALNL NPTLALIEKL
60 70 80 90 100
GGTKKRNEKK IKLDEFLPIY SQVKKEKEQG CYEDFIECLK LYDKEENGTM
110 120 130 140 150
LLAELQHALL ALGESLDDEQ VETLFADCMD PEDDEGFIPY SQFVQRLMSD

PVVFD
Length:155
Mass (Da):17,524
Last modified:January 27, 2003 - v4
Checksum:i65C92CD17995ECF5
GO
Isoform Indirect flight muscle (identifier: P06742-2) [UniParc]FASTAAdd to basket

Also known as: Pupa, Adult flight muscle

The sequence of this isoform differs from the canonical sequence as follows:
     142-155: QFVQRLMSDPVVFD → PFLARMCDRPDQLK

Show »
Length:155
Mass (Da):17,533
Checksum:i5B12DC95EB8A16ED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21A → V (PubMed:2982157).Curated
Sequence conflicti67 – 671L → M in AAA53454 (PubMed:7535717).Curated
Sequence conflicti67 – 671L → M in AAA53458 (PubMed:7535717).Curated
Sequence conflicti67 – 671L → M in AAA53460 (PubMed:7535717).Curated
Sequence conflicti67 – 671L → M in AAA53466 (PubMed:7535717).Curated
Sequence conflicti67 – 671L → M in AAA53468 (PubMed:7535717).Curated
Sequence conflicti150 – 1501D → H (PubMed:2982157).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei142 – 15514QFVQR…PVVFD → PFLARMCDRPDQLK in isoform Indirect flight muscle. CuratedVSP_003367Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10125 Genomic DNA. Translation: AAA28711.1.
M10125 Genomic DNA. Translation: AAA28712.1.
K01567 mRNA. Translation: AAA28710.1.
AE014297 Genomic DNA. Translation: AAF56733.2.
AE014297 Genomic DNA. Translation: AAN14121.1.
AY070972 mRNA. Translation: AAL48594.1.
L37312 Genomic DNA. Translation: AAA53441.1.
L37312 Genomic DNA. Translation: AAA53442.1.
L37313 Genomic DNA. Translation: AAA53443.1.
L37313 Genomic DNA. Translation: AAA53444.1.
L37314 Genomic DNA. Translation: AAA53445.1.
L37314 Genomic DNA. Translation: AAA53446.1.
L37315 Genomic DNA. Translation: AAA53447.1. Sequence problems.
L37315 Genomic DNA. Translation: AAA53448.1. Sequence problems.
L37316 Genomic DNA. Translation: AAA56798.1.
L37316 Genomic DNA. Translation: AAA56799.1. Sequence problems.
L37317 Genomic DNA. Translation: AAA53449.1.
L37317 Genomic DNA. Translation: AAA53450.1.
L37318 Genomic DNA. Translation: AAA53451.1.
L37318 Genomic DNA. Translation: AAA53452.1.
L37319 Genomic DNA. Translation: AAA53453.1.
L37319 Genomic DNA. Translation: AAA53454.1.
L37320 Genomic DNA. Translation: AAA53455.1.
L37320 Genomic DNA. Translation: AAA53456.1.
L37321 Genomic DNA. Translation: AAA53457.1.
L37321 Genomic DNA. Translation: AAA53458.1.
L37322 Genomic DNA. Translation: AAA53459.1.
L37322 Genomic DNA. Translation: AAA53460.1.
L37323 Genomic DNA. Translation: AAA53461.1.
L37323 Genomic DNA. Translation: AAA53462.1.
L37324 Genomic DNA. Translation: AAA53463.1.
L37324 Genomic DNA. Translation: AAA53464.1.
L37325 Genomic DNA. Translation: AAA53465.1.
L37325 Genomic DNA. Translation: AAA53466.1.
L37326 Genomic DNA. Translation: AAA53467.1.
L37326 Genomic DNA. Translation: AAA53468.1.
L37327 Genomic DNA. Translation: AAA53469.1.
L37327 Genomic DNA. Translation: AAA53470.1.
RefSeqiNP_001287569.1. NM_001300640.1. [P06742-1]
NP_476639.1. NM_057291.4. [P06742-2]
NP_476640.1. NM_057292.4. [P06742-1]
UniGeneiDm.6719.

Genome annotation databases

EnsemblMetazoaiFBtr0085196; FBpp0084566; FBgn0002772. [P06742-1]
FBtr0344131; FBpp0310544; FBgn0002772. [P06742-1]
GeneIDi43323.
KEGGidme:Dmel_CG5596.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10125 Genomic DNA. Translation: AAA28711.1.
M10125 Genomic DNA. Translation: AAA28712.1.
K01567 mRNA. Translation: AAA28710.1.
AE014297 Genomic DNA. Translation: AAF56733.2.
AE014297 Genomic DNA. Translation: AAN14121.1.
AY070972 mRNA. Translation: AAL48594.1.
L37312 Genomic DNA. Translation: AAA53441.1.
L37312 Genomic DNA. Translation: AAA53442.1.
L37313 Genomic DNA. Translation: AAA53443.1.
L37313 Genomic DNA. Translation: AAA53444.1.
L37314 Genomic DNA. Translation: AAA53445.1.
L37314 Genomic DNA. Translation: AAA53446.1.
L37315 Genomic DNA. Translation: AAA53447.1. Sequence problems.
L37315 Genomic DNA. Translation: AAA53448.1. Sequence problems.
L37316 Genomic DNA. Translation: AAA56798.1.
L37316 Genomic DNA. Translation: AAA56799.1. Sequence problems.
L37317 Genomic DNA. Translation: AAA53449.1.
L37317 Genomic DNA. Translation: AAA53450.1.
L37318 Genomic DNA. Translation: AAA53451.1.
L37318 Genomic DNA. Translation: AAA53452.1.
L37319 Genomic DNA. Translation: AAA53453.1.
L37319 Genomic DNA. Translation: AAA53454.1.
L37320 Genomic DNA. Translation: AAA53455.1.
L37320 Genomic DNA. Translation: AAA53456.1.
L37321 Genomic DNA. Translation: AAA53457.1.
L37321 Genomic DNA. Translation: AAA53458.1.
L37322 Genomic DNA. Translation: AAA53459.1.
L37322 Genomic DNA. Translation: AAA53460.1.
L37323 Genomic DNA. Translation: AAA53461.1.
L37323 Genomic DNA. Translation: AAA53462.1.
L37324 Genomic DNA. Translation: AAA53463.1.
L37324 Genomic DNA. Translation: AAA53464.1.
L37325 Genomic DNA. Translation: AAA53465.1.
L37325 Genomic DNA. Translation: AAA53466.1.
L37326 Genomic DNA. Translation: AAA53467.1.
L37326 Genomic DNA. Translation: AAA53468.1.
L37327 Genomic DNA. Translation: AAA53469.1.
L37327 Genomic DNA. Translation: AAA53470.1.
RefSeqiNP_001287569.1. NM_001300640.1. [P06742-1]
NP_476639.1. NM_057291.4. [P06742-2]
NP_476640.1. NM_057292.4. [P06742-1]
UniGeneiDm.6719.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QBDX-ray2.23B/D1-151[»]
ProteinModelPortaliP06742.
SMRiP06742. Positions 1-151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68205. 46 interactions.
DIPiDIP-23903N.
IntActiP06742. 20 interactions.
MINTiMINT-782616.
STRINGi7227.FBpp0084565.

Proteomic databases

PaxDbiP06742.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0085196; FBpp0084566; FBgn0002772. [P06742-1]
FBtr0344131; FBpp0310544; FBgn0002772. [P06742-1]
GeneIDi43323.
KEGGidme:Dmel_CG5596.

Organism-specific databases

CTDi23209.
FlyBaseiFBgn0002772. Mlc1.

Phylogenomic databases

eggNOGiENOG410ISFW. Eukaryota.
ENOG4111WV2. LUCA.
GeneTreeiENSGT00590000082921.
InParanoidiP06742.
OMAiMDEFLPI.
OrthoDBiEOG73NG53.
PhylomeDBiP06742.

Enzyme and pathway databases

ReactomeiR-DME-445355. Smooth Muscle Contraction.
R-DME-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

ChiTaRSiMlc1. fly.
GenomeRNAii43323.
PROiP06742.

Gene expression databases

BgeeiP06742.
ExpressionAtlasiP06742. differential.
GenevisibleiP06742. DM.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Developmental variations in the splicing pattern of transcripts from the Drosophila gene encoding myosin alkali light chain result in different carboxyl-terminal amino acid sequences."
    Falkenthal S., Parker V.P., Davidson N.
    Proc. Natl. Acad. Sci. U.S.A. 82:449-453(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE.
    Tissue: Embryo, Larva and Pupae.
  2. "Drosophila melanogaster has only one myosin alkali light-chain gene which encodes a protein with considerable amino acid sequence homology to chicken myosin alkali light chains."
    Falkenthal S., Parker V.P., Mattox W.W., Davidson N.
    Mol. Cell. Biol. 4:956-965(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LARVAL-ADULT), DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: Canton-S.
    Tissue: Embryo, Flight muscle and Pupae.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LARVAL-ADULT).
    Strain: Berkeley.
    Tissue: Embryo.
  6. "Constraints on intron evolution in the gene encoding the myosin alkali light chain in Drosophila."
    Leicht B.G., Muse S.V., Hanczyc M., Clark A.G.
    Genetics 139:299-308(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-155, ALTERNATIVE SPLICING.
    Strain: M171, M174, M179, M180, M192, M219, M223, M234, M237, M240, M241N, M242, M245, M247, M249 and M86.
  7. "The indirect flight muscle of Drosophila accumulates a unique myosin alkali light chain isoform."
    Falkenthal S., Graham M., Wilkinson J.
    Dev. Biol. 121:263-272(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
    Tissue: Abdomen, Flight muscle, Head, Muscle and Pupae.

Entry informationi

Entry nameiMLC1_DROME
AccessioniPrimary (citable) accession number: P06742
Secondary accession number(s): P06743
, P92155, P92156, P92157, P92183, P92187, P92196, Q24380, Q24381, Q24382, Q24383, Q27304, Q27315, Q27381, Q27414, Q94988, Q9VB12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 27, 2003
Last modified: July 6, 2016
This is version 149 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.