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Protein

Creatine kinase M-type

Gene

CKM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191ATPPROSITE-ProRule annotation1
Binding sitei236ATPPROSITE-ProRule annotation1
Binding sitei292ATPPROSITE-ProRule annotation1
Binding sitei335ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 132ATPPROSITE-ProRule annotation5
Nucleotide bindingi320 – 325ATPPROSITE-ProRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS02640-MONOMER.
ZFISH:HS02640-MONOMER.
BRENDAi2.7.3.2. 2681.
ReactomeiR-HSA-71288. Creatine metabolism.
SABIO-RKP06732.

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase M-type (EC:2.7.3.2)
Alternative name(s):
Creatine kinase M chain
M-CK
Cleaved into the following chain:
Gene namesi
Name:CKM
Synonyms:CKMM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:1994. CKM.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi1158.
OpenTargetsiENSG00000104879.
PharmGKBiPA26532.

Chemistry databases

ChEMBLiCHEMBL2656.
DrugBankiDB00148. Creatine.

Polymorphism and mutation databases

BioMutaiCKM.
DMDMi125305.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002119751 – 381Creatine kinase M-typeAdd BLAST381
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004217882 – 381Creatine kinase M-type, N-terminally processedAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei164PhosphoserineBy similarity1
Modified residuei166PhosphothreonineBy similarity1
Modified residuei178PhosphoserineBy similarity1
Modified residuei180PhosphothreonineBy similarity1
Modified residuei199PhosphoserineBy similarity1
Modified residuei313PhosphothreonineBy similarity1
Modified residuei322PhosphothreonineBy similarity1
Modified residuei332PhosphoserineBy similarity1
Modified residuei372PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP06732.
MaxQBiP06732.
PaxDbiP06732.
PeptideAtlasiP06732.
PRIDEiP06732.

2D gel databases

UCD-2DPAGEP06732.

PTM databases

iPTMnetiP06732.
PhosphoSitePlusiP06732.

Expressioni

Gene expression databases

BgeeiENSG00000104879.
CleanExiHS_CKM.
GenevisibleiP06732. HS.

Organism-specific databases

HPAiHPA047859.

Interactioni

Subunit structurei

Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Binary interactionsi

WithEntry#Exp.IntActNotes
ASB9Q96DX56EBI-4287089,EBI-745641

Protein-protein interaction databases

BioGridi107578. 23 interactors.
IntActiP06732. 5 interactors.
STRINGi9606.ENSP00000221476.

Chemistry databases

BindingDBiP06732.

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 19Combined sources4
Helixi29 – 33Combined sources5
Helixi36 – 42Combined sources7
Helixi53 – 62Combined sources10
Beta strandi67 – 69Combined sources3
Helixi80 – 84Combined sources5
Helixi86 – 93Combined sources8
Turni94 – 96Combined sources3
Beta strandi97 – 99Combined sources3
Turni123 – 125Combined sources3
Beta strandi126 – 132Combined sources7
Turni143 – 145Combined sources3
Helixi148 – 164Combined sources17
Helixi167 – 169Combined sources3
Beta strandi171 – 176Combined sources6
Turni181 – 183Combined sources3
Helixi184 – 189Combined sources6
Turni200 – 206Combined sources7
Turni209 – 214Combined sources6
Beta strandi216 – 220Combined sources5
Beta strandi223 – 244Combined sources22
Helixi246 – 266Combined sources21
Turni275 – 277Combined sources3
Helixi284 – 286Combined sources3
Beta strandi292 – 298Combined sources7
Turni300 – 304Combined sources5
Helixi308 – 314Combined sources7
Beta strandi333 – 338Combined sources6
Beta strandi342 – 344Combined sources3
Helixi346 – 368Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I0EX-ray3.50A/B/C/D1-381[»]
ProteinModelPortaliP06732.
SMRiP06732.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06732.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 98Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST88
Domaini125 – 367Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP06732.
KOiK00933.
OMAiNIRFTEK.
OrthoDBiEOG091G0HZ0.
PhylomeDBiP06732.
TreeFamiTF314214.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06732-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFGNTHNKF KLNYKPEEEY PDLSKHNNHM AKVLTLELYK KLRDKETPSG
60 70 80 90 100
FTVDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PIISDRHGGY
110 120 130 140 150
KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE
160 170 180 190 200
RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EKEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF
260 270 280 290 300
RRFCVGLQKI EEIFKKAGHP FMWNQHLGYV LTCPSNLGTG LRGGVHVKLA
310 320 330 340 350
HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV
360 370 380
QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ K
Length:381
Mass (Da):43,101
Last modified:November 1, 1990 - v2
Checksum:i418FEAD0C2E138C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47T → I in AAA52025 (PubMed:3778496).Curated1
Sequence conflicti130R → P in AAA52025 (PubMed:3778496).Curated1
Sequence conflicti193L → Q in AAA52025 (PubMed:3778496).Curated1
Sequence conflicti210D → H in AAA52025 (PubMed:3778496).Curated1
Sequence conflicti215R → P in AAA52025 (PubMed:3778496).Curated1
Sequence conflicti225F → L in AAP35439 (Ref. 3) Curated1
Sequence conflicti225F → L in AAH07462 (PubMed:15489334).Curated1
Sequence conflicti324G → A in AAA52025 (PubMed:3778496).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01868083E → G.1 PublicationCorresponds to variant rs11559024dbSNPEnsembl.1
Natural variantiVAR_018681127L → V.1 PublicationCorresponds to variant rs17875653dbSNPEnsembl.1
Natural variantiVAR_049675166T → M.Corresponds to variant rs17357122dbSNPEnsembl.1
Natural variantiVAR_018682243G → A.1 PublicationCorresponds to variant rs17875625dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14780 mRNA. Translation: AAA52025.1.
M21494
, M21488, M21489, M21490, M21491, M21492, M21493 Genomic DNA. Translation: AAA96609.1.
BT006793 mRNA. Translation: AAP35439.1.
AY585238 Genomic DNA. Translation: AAS79321.1.
AC005781 Genomic DNA. Translation: AAC62841.1.
BC007462 mRNA. Translation: AAH07462.1.
M16440 mRNA. Translation: AAA52026.1. Sequence problems.
CCDSiCCDS12659.1.
PIRiA31793. KIHUCM.
RefSeqiNP_001815.2. NM_001824.4.
XP_016881729.1. XM_017026240.1.
UniGeneiHs.334347.

Genome annotation databases

EnsembliENST00000221476; ENSP00000221476; ENSG00000104879.
GeneIDi1158.
KEGGihsa:1158.
UCSCiuc002pbd.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Creatine kinase entry

SeattleSNPs
Wikipedia

CKM entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14780 mRNA. Translation: AAA52025.1.
M21494
, M21488, M21489, M21490, M21491, M21492, M21493 Genomic DNA. Translation: AAA96609.1.
BT006793 mRNA. Translation: AAP35439.1.
AY585238 Genomic DNA. Translation: AAS79321.1.
AC005781 Genomic DNA. Translation: AAC62841.1.
BC007462 mRNA. Translation: AAH07462.1.
M16440 mRNA. Translation: AAA52026.1. Sequence problems.
CCDSiCCDS12659.1.
PIRiA31793. KIHUCM.
RefSeqiNP_001815.2. NM_001824.4.
XP_016881729.1. XM_017026240.1.
UniGeneiHs.334347.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I0EX-ray3.50A/B/C/D1-381[»]
ProteinModelPortaliP06732.
SMRiP06732.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107578. 23 interactors.
IntActiP06732. 5 interactors.
STRINGi9606.ENSP00000221476.

Chemistry databases

BindingDBiP06732.
ChEMBLiCHEMBL2656.
DrugBankiDB00148. Creatine.

PTM databases

iPTMnetiP06732.
PhosphoSitePlusiP06732.

Polymorphism and mutation databases

BioMutaiCKM.
DMDMi125305.

2D gel databases

UCD-2DPAGEP06732.

Proteomic databases

EPDiP06732.
MaxQBiP06732.
PaxDbiP06732.
PeptideAtlasiP06732.
PRIDEiP06732.

Protocols and materials databases

DNASUi1158.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000221476; ENSP00000221476; ENSG00000104879.
GeneIDi1158.
KEGGihsa:1158.
UCSCiuc002pbd.5. human.

Organism-specific databases

CTDi1158.
DisGeNETi1158.
GeneCardsiCKM.
HGNCiHGNC:1994. CKM.
HPAiHPA047859.
MIMi123310. gene.
neXtProtiNX_P06732.
OpenTargetsiENSG00000104879.
PharmGKBiPA26532.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP06732.
KOiK00933.
OMAiNIRFTEK.
OrthoDBiEOG091G0HZ0.
PhylomeDBiP06732.
TreeFamiTF314214.

Enzyme and pathway databases

BioCyciMetaCyc:HS02640-MONOMER.
ZFISH:HS02640-MONOMER.
BRENDAi2.7.3.2. 2681.
ReactomeiR-HSA-71288. Creatine metabolism.
SABIO-RKP06732.

Miscellaneous databases

ChiTaRSiCKM. human.
EvolutionaryTraceiP06732.
GeneWikiiCKM_(gene).
GenomeRNAii1158.
PROiP06732.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000104879.
CleanExiHS_CKM.
GenevisibleiP06732. HS.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCRM_HUMAN
AccessioniPrimary (citable) accession number: P06732
Secondary accession number(s): Q96QL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1990
Last modified: November 30, 2016
This is version 173 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.