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Protein

65 kDa phosphoprotein

Gene

UL83

Organism
Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Counteracts the host antiviral immune response when activated and phosphorylated, by preventing IRF3 from entering the nucleus. Also participates in the transactivation of viral major immediate-early genes by the recruitment of host IFI16 to the promoters pf these genes.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host RLR pathway by virus, Viral immunoevasion

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
65 kDa phosphoprotein
Short name:
pp65
Alternative name(s):
65 kDa matrix phosphoprotein
Phosphoprotein UL83
Tegument protein UL83
Gene namesi
Name:UL83
OrganismiHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Taxonomic identifieri10360 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008992 Componenti: Genome
  • UP000008991 Componenti: Genome

Subcellular locationi

  • Virion tegument Curated
  • Host nucleus 2 Publications
  • Host cytoplasm 1 Publication

  • Note: As part of the incoming virion, pp65 is targeted to the nucleus immediately after infection (PubMed:7815485). The newly synthesized pp65 is observed in the nucleus until some time after 48 hours postinfection (PubMed:17715235). Thereafter, pp65 is probably exported and accumulates in the cytoplasm (PubMed:17715235). Also found in dense bodies (PubMed:7815485).

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001162921 – 56165 kDa phosphoproteinAdd BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei472PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation may play a role in the localization of the protein.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP06725.

Interactioni

Subunit structurei

Interacts with host NCL/nucleolin. Interacts with host IFI16.2 Publications

Protein-protein interaction databases

DIPiDIP-61111N.
IntActiP06725. 3 interactors.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X4RX-ray2.30C/F495-503[»]
2X4TX-ray2.30C/F495-503[»]
3GSOX-ray1.60P495-503[»]
SMRiP06725.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06725.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi537 – 560Bipartite nuclear localization signalCuratedAdd BLAST24

Sequence similaritiesi

Belongs to the herpesviridae pp65 family.Curated

Family and domain databases

InterProiIPR029054. dUTPase-like.
IPR008649. Herpes_UL82/UL83.
[Graphical view]
PfamiPF05784. Herpes_UL82_83. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.

Sequencei

Sequence statusi: Complete.

P06725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRGRRCPE MISVLGPISG HVLKAVFSRG DTPVLPHETR LLQTGIHVRV
60 70 80 90 100
SQPSLILVSQ YTPDSTPCHR GDNQLQVQHT YFTGSEVENV SVNVHNPTGR
110 120 130 140 150
SICPSQEPMS IYVYALPLKM LNIPSINVHH YPSAAERKHR HLPVADAVIH
160 170 180 190 200
ASGKQMWQAR LTVSGLAWTR QQNQWKEPDV YYTSAFVFPT KDVALRHVVC
210 220 230 240 250
AHELVCSMEN TRATKMQVIG DQYVKVYLES FCEDVPSGKL FMHVTLGSDV
260 270 280 290 300
EEDLTMTRNP QPFMRPHERN GFTVLCPKNM IIKPGKISHI MLDVAFTSHE
310 320 330 340 350
HFGLLCPKSI PGLSISGNLL MNGQQIFLEV QAIRETVELR QYDPVAALFF
360 370 380 390 400
FDIDLLLQRG PQYSEHPTFT SQYRIQGKLE YRHTWDRHDE GAAQGDDDVW
410 420 430 440 450
TSGSDSDEEL VTTERKTPRV TGGGAMAGAS TSAGRKRKSA SSATACTSGV
460 470 480 490 500
MTRGRLKAES TVAPEEDTDE DSDNEIHNPA VFTWPPWQAG ILARNLVPMV
510 520 530 540 550
ATVQGQNLKY QEFFWDANDI YRIFAELEGV WQPAAQPKRR RHRQDALPGP
560
CIASTPKKHR G
Length:561
Mass (Da):62,898
Last modified:July 1, 1989 - v2
Checksum:i37422EA149E88F30
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94 – 106Missing in AAA45996 (PubMed:2161319).CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15120 Genomic DNA. Translation: AAA45996.1. Sequence problems.
X17403 Genomic DNA. Translation: CAA35357.1.
K02531 Genomic DNA. Translation: AAA45983.1.
BK000394 Genomic DNA. Translation: DAA00180.1.
PIRiA26793. WMBE65.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15120 Genomic DNA. Translation: AAA45996.1. Sequence problems.
X17403 Genomic DNA. Translation: CAA35357.1.
K02531 Genomic DNA. Translation: AAA45983.1.
BK000394 Genomic DNA. Translation: DAA00180.1.
PIRiA26793. WMBE65.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X4RX-ray2.30C/F495-503[»]
2X4TX-ray2.30C/F495-503[»]
3GSOX-ray1.60P495-503[»]
SMRiP06725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61111N.
IntActiP06725. 3 interactors.

Proteomic databases

PRIDEiP06725.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

EvolutionaryTraceiP06725.

Family and domain databases

InterProiIPR029054. dUTPase-like.
IPR008649. Herpes_UL82/UL83.
[Graphical view]
PfamiPF05784. Herpes_UL82_83. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPP65_HCMVA
AccessioniPrimary (citable) accession number: P06725
Secondary accession number(s): Q7M6K8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.