P06725 (PP65_HCMVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
65 kDa phosphoprotein
Short name=pp65

Alternative name(s):
65 kDa matrix phosphoprotein
Phosphoprotein UL83
Tegument protein UL83

Gene names

Name:

UL83

Organism

Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5) [Complete proteome]

Taxonomic identifier

10360 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Betaherpesvirinae › Cytomegalovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Virion tegument Potential. Host nucleus. Host cytoplasm. Note: As part of the incoming virion, pp65 is targeted to the nucleus immediately after infection. The newly synthesized pp65 is observed in the nucleus until some time after 48 hours postinfection. Thereafter, pp65 is probably exported and accumulates in the cytoplasm. Also found in dense bodies. Ref.6 Ref.9
Post-translational modification	Phosphorylation may play a role in the localization of the protein.
Sequence similarities	Belongs to the herpesviridae pp65 family.

Ontologies

Keywords
Cellular component	Host cytoplasm Host nucleus Virion Virion tegument
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral tegument Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	65 kDa phosphoprotein		PRO_0000116292
Regions
	Motif	537 – 560	24	Bipartite nuclear localization signal Probable
Amino acid modifications
	Modified residue	472	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	94 – 106	13	Missing in AAA45996. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P06725 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: 37422EA149E88F30	FASTA	561	62,898
	10 20 30 40 50 60 MESRGRRCPE MISVLGPISG HVLKAVFSRG DTPVLPHETR LLQTGIHVRV SQPSLILVSQ 70 80 90 100 110 120 YTPDSTPCHR GDNQLQVQHT YFTGSEVENV SVNVHNPTGR SICPSQEPMS IYVYALPLKM 130 140 150 160 170 180 LNIPSINVHH YPSAAERKHR HLPVADAVIH ASGKQMWQAR LTVSGLAWTR QQNQWKEPDV 190 200 210 220 230 240 YYTSAFVFPT KDVALRHVVC AHELVCSMEN TRATKMQVIG DQYVKVYLES FCEDVPSGKL 250 260 270 280 290 300 FMHVTLGSDV EEDLTMTRNP QPFMRPHERN GFTVLCPKNM IIKPGKISHI MLDVAFTSHE 310 320 330 340 350 360 HFGLLCPKSI PGLSISGNLL MNGQQIFLEV QAIRETVELR QYDPVAALFF FDIDLLLQRG 370 380 390 400 410 420 PQYSEHPTFT SQYRIQGKLE YRHTWDRHDE GAAQGDDDVW TSGSDSDEEL VTTERKTPRV 430 440 450 460 470 480 TGGGAMAGAS TSAGRKRKSA SSATACTSGV MTRGRLKAES TVAPEEDTDE DSDNEIHNPA 490 500 510 520 530 540 VFTWPPWQAG ILARNLVPMV ATVQGQNLKY QEFFWDANDI YRIFAELEGV WQPAAQPKRR 550 560 RHRQDALPGP CIASTPKKHR G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure and transcription of the genes coding for the two virion phosphoproteins pp65 and pp71 of human cytomegalovirus." Rueger B., Klages S., Walla B., Albrecht J.-C., Fleckenstein B., Tomlinson P., Barrell B.G. J. Virol. 61:446-453(1987) [PubMed: 3027374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169." Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R., Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A., Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G. Curr. Top. Microbiol. Immunol. 154:125-169(1990) [PubMed: 2161319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Cloning and physical mapping of a gene fragment coding for a 64-kilodalton major late antigen of human cytomegalovirus." Pande H., Baak S.W., Riggs A.D., Clark B.R., Shively J.E., Zaia J.A. Proc. Natl. Acad. Sci. U.S.A. 81:4965-4969(1984) [PubMed: 6087357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 495-538.
[4]	"The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome." Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S. J. Gen. Virol. 84:17-28(2003) [PubMed: 12533697] [Abstract] Cited for: GENOME REANNOTATION.
[5]	Erratum Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S. J. Gen. Virol. 84:1053-1053(2003)
[6]	"Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal functions with other portions of the protein to mediate its efficient nuclear transport." Schmolke S., Drescher P., Jahn G., Plachter B. J. Virol. 69:1071-1078(1995) [PubMed: 7815485] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome." Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J., Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W., Shenk T., Smith R.D., Nelson J.A. J. Virol. 78:10960-10966(2004) [PubMed: 15452216] [Abstract] Cited for: IDENTIFICATION.
[8]	Erratum Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J., Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W., Shenk T., Smith R.D., Nelson J.A. J. Virol. 78:13395-13395(2004)
[9]	"Nuclear export of the human cytomegalovirus tegument protein pp65 requires cyclin-dependent kinase activity and the Crm1 exporter." Sanchez V., Mahr J.A., Orazio N.I., Spector D.H. J. Virol. 81:11730-11736(2007) [PubMed: 17715235] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M15120 Genomic DNA. Translation: AAA45996.1. Sequence problems.
X17403 Genomic DNA. Translation: CAA35357.1.
K02531 Genomic DNA. Translation: AAA45983.1.
BK000394 Genomic DNA. Translation: DAA00180.1.

PIR

WMBE65. A26793.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2X4R	X-ray	2.30	C/F	495-503	[»]

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR008649. Herpes_UL82/UL83.
[Graphical view]

Pfam

PF05784. Herpes_UL82_83. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

PP65_HCMVA

Accession

Primary (citable) accession number: P06725
Secondary accession number(s): Q7M6K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	July 1, 1989
Last modified:	October 5, 2010
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program