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Protein

DNA mismatch repair protein MutH

Gene

mutH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair.

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • endonuclease activity Source: EcoliWiki
  • T/G mismatch-specific endonuclease activity Source: EcoliWiki

GO - Biological processi

  • DNA modification Source: InterPro
  • mismatch repair Source: EcoliWiki
  • regulation of DNA recombination Source: EcoliWiki

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciEcoCyc:EG10624-MONOMER
MetaCyc:EG10624-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MutHUniRule annotation
Alternative name(s):
Methyl-directed mismatch repair proteinUniRule annotation
Gene namesi
Name:mutHUniRule annotation
Synonyms:mutR, prv
Ordered Locus Names:b2831, JW2799
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10624 mutH

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • mismatch repair complex Source: EcoliWiki

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28T → L in TS7; temperature-sensitive. 1
Mutagenesisi157D → N in TS28; temperature-sensitive. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001986652 – 229DNA mismatch repair protein MutHAdd BLAST228

Proteomic databases

PaxDbiP06722
PRIDEiP06722

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4263275, 194 interactors
851627, 1 interactor
DIPiDIP-10284N
IntActiP06722, 10 interactors
STRINGi316385.ECDH10B_3001

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 23Combined sources10
Turni24 – 26Combined sources3
Helixi29 – 35Combined sources7
Turni44 – 46Combined sources3
Helixi50 – 58Combined sources9
Helixi69 – 72Combined sources4
Beta strandi77 – 83Combined sources7
Beta strandi93 – 97Combined sources5
Beta strandi100 – 102Combined sources3
Turni108 – 110Combined sources3
Helixi112 – 116Combined sources5
Beta strandi118 – 126Combined sources9
Helixi133 – 135Combined sources3
Beta strandi142 – 144Combined sources3
Helixi148 – 166Combined sources19
Helixi170 – 172Combined sources3
Beta strandi179 – 184Combined sources6
Beta strandi194 – 197Combined sources4
Beta strandi203 – 206Combined sources4
Beta strandi210 – 213Combined sources4
Helixi215 – 224Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AZOX-ray1.70A1-229[»]
2AZOX-ray2.30A/B1-229[»]
ProteinModelPortaliP06722
SMRiP06722
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06722

Family & Domainsi

Sequence similaritiesi

Belongs to the MutH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105IRT Bacteria
COG3066 LUCA
KOiK03573
OMAiPPDLKRD

Family and domain databases

CDDicd00583 MutH_Sau3AI, 1 hit
Gene3Di3.40.600.10, 1 hit
HAMAPiMF_00759 MutH, 1 hit
InterProiView protein in InterPro
IPR004230 DNA_mismatch_repair_MutH
IPR011337 DNA_rep_MutH/RE_typeII
IPR037057 DNA_rep_MutH/RE_typeII_sf
IPR011335 Restrct_endonuc-II-like
PfamiView protein in Pfam
PF02976 MutH, 1 hit
SMARTiView protein in SMART
SM00927 MutH, 1 hit
SUPFAMiSSF52980 SSF52980, 1 hit
TIGRFAMsiTIGR02248 mutH_TIGR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPRPLLSP PETEEQLLAQ AQQLSGYTLG ELAALVGLVT PENLKRDKGW
60 70 80 90 100
IGVLLEIWLG ASAGSKPEQD FAALGVELKT IPVDSLGRPL ETTFVCVAPL
110 120 130 140 150
TGNSGVTWET SHVRHKLKRV LWIPVEGERS IPLAQRRVGS PLLWSPNEEE
160 170 180 190 200
DRQLREDWEE LMDMIVLGQV ERITARHGEY LQIRPKAANA KALTEAIGAR
210 220
GERILTLPRG FYLKKNFTSA LLARHFLIQ
Length:229
Mass (Da):25,527
Last modified:January 23, 2007 - v3
Checksum:i32543989D710A8FD
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti58W → S in strain: ECOR 67. 1
Natural varianti60G → C in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70. 1
Natural varianti64G → R in strain: ECOR 50. 1
Natural varianti135Q → K in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64. 1
Natural varianti153Q → R in strain: ECOR 67. 1
Natural varianti155R → C in strain: ECOR 35. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00113 Genomic DNA Translation: CAA68292.1 Sequence problems.
U16361 Genomic DNA Translation: AAB59986.1
U29581 Genomic DNA Translation: AAB40478.1
U00096 Genomic DNA Translation: AAC75870.1
AP009048 Genomic DNA Translation: BAE76900.1
AJ005726 Genomic DNA Translation: CAB41510.1
AJ005727 Genomic DNA Translation: CAB41511.1
AJ005728 Genomic DNA Translation: CAB41512.1
AJ005729 Genomic DNA Translation: CAB41513.1
AJ005730 Genomic DNA Translation: CAB41514.1
AJ005731 Genomic DNA Translation: CAB41515.1
AJ005732 Genomic DNA Translation: CAB41516.1
AJ005733 Genomic DNA Translation: CAB41517.1
AJ005734 Genomic DNA Translation: CAB41518.1
AJ005735 Genomic DNA Translation: CAB41519.1
AJ005736 Genomic DNA Translation: CAB41520.1
AJ005737 Genomic DNA Translation: CAB41521.1
AJ005738 Genomic DNA Translation: CAB41522.1
AJ005739 Genomic DNA Translation: CAB41523.1
AJ005740 Genomic DNA Translation: CAB41524.1
AJ005743 Genomic DNA Translation: CAB41669.1
AJ005744 Genomic DNA Translation: CAB41670.1
AJ005745 Genomic DNA Translation: CAB41671.1
AJ005746 Genomic DNA Translation: CAB41696.1
AJ005747 Genomic DNA Translation: CAB41672.1
AJ005748 Genomic DNA Translation: CAB41673.1
AJ005749 Genomic DNA Translation: CAB41674.1
AJ005750 Genomic DNA Translation: CAB41675.1
AJ005751 Genomic DNA Translation: CAB41676.1
AJ005752 Genomic DNA Translation: CAB41677.1
AJ005753 Genomic DNA Translation: CAB41678.1
AJ005755 Genomic DNA Translation: CAB41679.1
AJ238722 Genomic DNA Translation: CAB91077.1
AJ238719 Genomic DNA Translation: CAB91074.1
AJ238720 Genomic DNA Translation: CAB91075.1
AJ238721 Genomic DNA Translation: CAB91076.1
PIRiH65065 MVECMH
RefSeqiNP_417308.1, NC_000913.3
WP_000082201.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75870; AAC75870; b2831
BAE76900; BAE76900; BAE76900
GeneIDi947299
KEGGiecj:JW2799
eco:b2831
PATRICifig|1411691.4.peg.3903

Similar proteinsi

Entry informationi

Entry nameiMUTH_ECOLI
AccessioniPrimary (citable) accession number: P06722
Secondary accession number(s): Q2MA06
, Q9R2X2, Q9R3A8, Q9R411, Q9S6P5, Q9S6P6, Q9S6P7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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