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Protein

DNA mismatch repair protein MutH

Gene

mutH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair.

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • endonuclease activity Source: EcoliWiki
  • T/G mismatch-specific endonuclease activity Source: EcoliWiki

GO - Biological processi

  • DNA modification Source: InterPro
  • mismatch repair Source: EcoliWiki
  • regulation of DNA recombination Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciEcoCyc:EG10624-MONOMER.
ECOL316407:JW2799-MONOMER.
MetaCyc:EG10624-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MutHUniRule annotation
Alternative name(s):
Methyl-directed mismatch repair proteinUniRule annotation
Gene namesi
Name:mutHUniRule annotation
Synonyms:mutR, prv
Ordered Locus Names:b2831, JW2799
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10624. mutH.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • mismatch repair complex Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi28 – 281T → L in TS7; temperature-sensitive.
Mutagenesisi157 – 1571D → N in TS28; temperature-sensitive.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 229228DNA mismatch repair protein MutHPRO_0000198665Add
BLAST

Proteomic databases

PaxDbiP06722.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
mutLP233678EBI-545170,EBI-554913

Protein-protein interaction databases

BioGridi4263275. 194 interactions.
DIPiDIP-10284N.
IntActiP06722. 10 interactions.
MINTiMINT-1542547.
STRINGi511145.b2831.

Structurei

Secondary structure

1
229
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 2310Combined sources
Turni24 – 263Combined sources
Helixi29 – 357Combined sources
Turni44 – 463Combined sources
Helixi50 – 589Combined sources
Helixi69 – 724Combined sources
Beta strandi77 – 837Combined sources
Beta strandi93 – 975Combined sources
Beta strandi100 – 1023Combined sources
Turni108 – 1103Combined sources
Helixi112 – 1165Combined sources
Beta strandi118 – 1269Combined sources
Helixi133 – 1353Combined sources
Beta strandi142 – 1443Combined sources
Helixi148 – 16619Combined sources
Helixi170 – 1723Combined sources
Beta strandi179 – 1846Combined sources
Beta strandi194 – 1974Combined sources
Beta strandi203 – 2064Combined sources
Beta strandi210 – 2134Combined sources
Helixi215 – 22410Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AZOX-ray1.70A1-229[»]
2AZOX-ray2.30A/B1-229[»]
ProteinModelPortaliP06722.
SMRiP06722. Positions 3-229.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06722.

Family & Domainsi

Sequence similaritiesi

Belongs to the MutH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105IRT. Bacteria.
COG3066. LUCA.
KOiK03573.
OMAiVMQLRPK.
OrthoDBiEOG6CGCC0.

Family and domain databases

Gene3Di3.40.600.10. 1 hit.
HAMAPiMF_00759. MutH.
InterProiIPR004230. DNA_mismatch_repair_MutH.
IPR011337. DNA_rep_MutH/RE_typeII.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF02976. MutH. 1 hit.
[Graphical view]
SMARTiSM00927. MutH. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
TIGRFAMsiTIGR02248. mutH_TIGR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPRPLLSP PETEEQLLAQ AQQLSGYTLG ELAALVGLVT PENLKRDKGW
60 70 80 90 100
IGVLLEIWLG ASAGSKPEQD FAALGVELKT IPVDSLGRPL ETTFVCVAPL
110 120 130 140 150
TGNSGVTWET SHVRHKLKRV LWIPVEGERS IPLAQRRVGS PLLWSPNEEE
160 170 180 190 200
DRQLREDWEE LMDMIVLGQV ERITARHGEY LQIRPKAANA KALTEAIGAR
210 220
GERILTLPRG FYLKKNFTSA LLARHFLIQ
Length:229
Mass (Da):25,527
Last modified:January 23, 2007 - v3
Checksum:i32543989D710A8FD
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti58 – 581W → S in strain: ECOR 67.
Natural varianti60 – 601G → C in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70.
Natural varianti64 – 641G → R in strain: ECOR 50.
Natural varianti135 – 1351Q → K in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64.
Natural varianti153 – 1531Q → R in strain: ECOR 67.
Natural varianti155 – 1551R → C in strain: ECOR 35.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00113 Genomic DNA. Translation: CAA68292.1. Sequence problems.
U16361 Genomic DNA. Translation: AAB59986.1.
U29581 Genomic DNA. Translation: AAB40478.1.
U00096 Genomic DNA. Translation: AAC75870.1.
AP009048 Genomic DNA. Translation: BAE76900.1.
AJ005726 Genomic DNA. Translation: CAB41510.1.
AJ005727 Genomic DNA. Translation: CAB41511.1.
AJ005728 Genomic DNA. Translation: CAB41512.1.
AJ005729 Genomic DNA. Translation: CAB41513.1.
AJ005730 Genomic DNA. Translation: CAB41514.1.
AJ005731 Genomic DNA. Translation: CAB41515.1.
AJ005732 Genomic DNA. Translation: CAB41516.1.
AJ005733 Genomic DNA. Translation: CAB41517.1.
AJ005734 Genomic DNA. Translation: CAB41518.1.
AJ005735 Genomic DNA. Translation: CAB41519.1.
AJ005736 Genomic DNA. Translation: CAB41520.1.
AJ005737 Genomic DNA. Translation: CAB41521.1.
AJ005738 Genomic DNA. Translation: CAB41522.1.
AJ005739 Genomic DNA. Translation: CAB41523.1.
AJ005740 Genomic DNA. Translation: CAB41524.1.
AJ005743 Genomic DNA. Translation: CAB41669.1.
AJ005744 Genomic DNA. Translation: CAB41670.1.
AJ005745 Genomic DNA. Translation: CAB41671.1.
AJ005746 Genomic DNA. Translation: CAB41696.1.
AJ005747 Genomic DNA. Translation: CAB41672.1.
AJ005748 Genomic DNA. Translation: CAB41673.1.
AJ005749 Genomic DNA. Translation: CAB41674.1.
AJ005750 Genomic DNA. Translation: CAB41675.1.
AJ005751 Genomic DNA. Translation: CAB41676.1.
AJ005752 Genomic DNA. Translation: CAB41677.1.
AJ005753 Genomic DNA. Translation: CAB41678.1.
AJ005755 Genomic DNA. Translation: CAB41679.1.
AJ238722 Genomic DNA. Translation: CAB91077.1.
AJ238719 Genomic DNA. Translation: CAB91074.1.
AJ238720 Genomic DNA. Translation: CAB91075.1.
AJ238721 Genomic DNA. Translation: CAB91076.1.
PIRiH65065. MVECMH.
RefSeqiNP_417308.1. NC_000913.3.
WP_000082201.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75870; AAC75870; b2831.
BAE76900; BAE76900; BAE76900.
GeneIDi947299.
KEGGiecj:JW2799.
eco:b2831.
PATRICi32121080. VBIEscCol129921_2929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00113 Genomic DNA. Translation: CAA68292.1. Sequence problems.
U16361 Genomic DNA. Translation: AAB59986.1.
U29581 Genomic DNA. Translation: AAB40478.1.
U00096 Genomic DNA. Translation: AAC75870.1.
AP009048 Genomic DNA. Translation: BAE76900.1.
AJ005726 Genomic DNA. Translation: CAB41510.1.
AJ005727 Genomic DNA. Translation: CAB41511.1.
AJ005728 Genomic DNA. Translation: CAB41512.1.
AJ005729 Genomic DNA. Translation: CAB41513.1.
AJ005730 Genomic DNA. Translation: CAB41514.1.
AJ005731 Genomic DNA. Translation: CAB41515.1.
AJ005732 Genomic DNA. Translation: CAB41516.1.
AJ005733 Genomic DNA. Translation: CAB41517.1.
AJ005734 Genomic DNA. Translation: CAB41518.1.
AJ005735 Genomic DNA. Translation: CAB41519.1.
AJ005736 Genomic DNA. Translation: CAB41520.1.
AJ005737 Genomic DNA. Translation: CAB41521.1.
AJ005738 Genomic DNA. Translation: CAB41522.1.
AJ005739 Genomic DNA. Translation: CAB41523.1.
AJ005740 Genomic DNA. Translation: CAB41524.1.
AJ005743 Genomic DNA. Translation: CAB41669.1.
AJ005744 Genomic DNA. Translation: CAB41670.1.
AJ005745 Genomic DNA. Translation: CAB41671.1.
AJ005746 Genomic DNA. Translation: CAB41696.1.
AJ005747 Genomic DNA. Translation: CAB41672.1.
AJ005748 Genomic DNA. Translation: CAB41673.1.
AJ005749 Genomic DNA. Translation: CAB41674.1.
AJ005750 Genomic DNA. Translation: CAB41675.1.
AJ005751 Genomic DNA. Translation: CAB41676.1.
AJ005752 Genomic DNA. Translation: CAB41677.1.
AJ005753 Genomic DNA. Translation: CAB41678.1.
AJ005755 Genomic DNA. Translation: CAB41679.1.
AJ238722 Genomic DNA. Translation: CAB91077.1.
AJ238719 Genomic DNA. Translation: CAB91074.1.
AJ238720 Genomic DNA. Translation: CAB91075.1.
AJ238721 Genomic DNA. Translation: CAB91076.1.
PIRiH65065. MVECMH.
RefSeqiNP_417308.1. NC_000913.3.
WP_000082201.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AZOX-ray1.70A1-229[»]
2AZOX-ray2.30A/B1-229[»]
ProteinModelPortaliP06722.
SMRiP06722. Positions 3-229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263275. 194 interactions.
DIPiDIP-10284N.
IntActiP06722. 10 interactions.
MINTiMINT-1542547.
STRINGi511145.b2831.

Proteomic databases

PaxDbiP06722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75870; AAC75870; b2831.
BAE76900; BAE76900; BAE76900.
GeneIDi947299.
KEGGiecj:JW2799.
eco:b2831.
PATRICi32121080. VBIEscCol129921_2929.

Organism-specific databases

EchoBASEiEB0619.
EcoGeneiEG10624. mutH.

Phylogenomic databases

eggNOGiENOG4105IRT. Bacteria.
COG3066. LUCA.
KOiK03573.
OMAiVMQLRPK.
OrthoDBiEOG6CGCC0.

Enzyme and pathway databases

BioCyciEcoCyc:EG10624-MONOMER.
ECOL316407:JW2799-MONOMER.
MetaCyc:EG10624-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06722.
PROiP06722.

Family and domain databases

Gene3Di3.40.600.10. 1 hit.
HAMAPiMF_00759. MutH.
InterProiIPR004230. DNA_mismatch_repair_MutH.
IPR011337. DNA_rep_MutH/RE_typeII.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF02976. MutH. 1 hit.
[Graphical view]
SMARTiSM00927. MutH. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
TIGRFAMsiTIGR02248. mutH_TIGR. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Escherichia coli mutH gene."
    Grafstrom R.H., Hoess R.H.
    Nucleic Acids Res. 15:3073-3084(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-25, MUTANTS TS7 AND TS28.
    Strain: K12.
  2. Feng G., Tsui H.T., Winkler M.E., Loh T.A., Marinus M.G.
    Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 83; 130-131 AND 136.
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Denamur E.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 58-157.
    Strain: ECOR 1, ECOR 10, ECOR 13, ECOR 17, ECOR 20, ECOR 23, ECOR 24, ECOR 26, ECOR 34, ECOR 35, ECOR 37, ECOR 4, ECOR 40, ECOR 41, ECOR 45, ECOR 47, ECOR 49, ECOR 51, ECOR 52, ECOR 57, ECOR 58, ECOR 59, ECOR 62, ECOR 64, ECOR 67, ECOR 68, ECOR 70 and O2:HN / ECOR 50 / P97 / UPEC.
  6. "Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases."
    Ban C., Yang W.
    EMBO J. 17:1526-1534(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Entry informationi

Entry nameiMUTH_ECOLI
AccessioniPrimary (citable) accession number: P06722
Secondary accession number(s): Q2MA06
, Q9R2X2, Q9R3A8, Q9R411, Q9S6P5, Q9S6P6, Q9S6P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.