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Protein

DNA mismatch repair protein MutH

Gene

mutH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair.

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • endonuclease activity Source: EcoliWiki
  • T/G mismatch-specific endonuclease activity Source: EcoliWiki

GO - Biological processi

  • DNA modification Source: InterPro
  • mismatch repair Source: EcoliWiki
  • regulation of DNA recombination Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciEcoCyc:EG10624-MONOMER.
ECOL316407:JW2799-MONOMER.
MetaCyc:EG10624-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MutHUniRule annotation
Alternative name(s):
Methyl-directed mismatch repair proteinUniRule annotation
Gene namesi
Name:mutHUniRule annotation
Synonyms:mutR, prv
Ordered Locus Names:b2831, JW2799
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10624. mutH.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • mismatch repair complex Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28T → L in TS7; temperature-sensitive. 1
Mutagenesisi157D → N in TS28; temperature-sensitive. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001986652 – 229DNA mismatch repair protein MutHAdd BLAST228

Proteomic databases

PaxDbiP06722.
PRIDEiP06722.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
mutLP233678EBI-545170,EBI-554913

Protein-protein interaction databases

BioGridi4263275. 194 interactors.
DIPiDIP-10284N.
IntActiP06722. 10 interactors.
MINTiMINT-1542547.
STRINGi511145.b2831.

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 23Combined sources10
Turni24 – 26Combined sources3
Helixi29 – 35Combined sources7
Turni44 – 46Combined sources3
Helixi50 – 58Combined sources9
Helixi69 – 72Combined sources4
Beta strandi77 – 83Combined sources7
Beta strandi93 – 97Combined sources5
Beta strandi100 – 102Combined sources3
Turni108 – 110Combined sources3
Helixi112 – 116Combined sources5
Beta strandi118 – 126Combined sources9
Helixi133 – 135Combined sources3
Beta strandi142 – 144Combined sources3
Helixi148 – 166Combined sources19
Helixi170 – 172Combined sources3
Beta strandi179 – 184Combined sources6
Beta strandi194 – 197Combined sources4
Beta strandi203 – 206Combined sources4
Beta strandi210 – 213Combined sources4
Helixi215 – 224Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AZOX-ray1.70A1-229[»]
2AZOX-ray2.30A/B1-229[»]
ProteinModelPortaliP06722.
SMRiP06722.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06722.

Family & Domainsi

Sequence similaritiesi

Belongs to the MutH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105IRT. Bacteria.
COG3066. LUCA.
KOiK03573.
OMAiVMQLRPK.

Family and domain databases

CDDicd00583. MutH_Sau3AI. 1 hit.
Gene3Di3.40.600.10. 1 hit.
HAMAPiMF_00759. MutH. 1 hit.
InterProiIPR004230. DNA_mismatch_repair_MutH.
IPR011337. DNA_rep_MutH/RE_typeII.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF02976. MutH. 1 hit.
[Graphical view]
SMARTiSM00927. MutH. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
TIGRFAMsiTIGR02248. mutH_TIGR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPRPLLSP PETEEQLLAQ AQQLSGYTLG ELAALVGLVT PENLKRDKGW
60 70 80 90 100
IGVLLEIWLG ASAGSKPEQD FAALGVELKT IPVDSLGRPL ETTFVCVAPL
110 120 130 140 150
TGNSGVTWET SHVRHKLKRV LWIPVEGERS IPLAQRRVGS PLLWSPNEEE
160 170 180 190 200
DRQLREDWEE LMDMIVLGQV ERITARHGEY LQIRPKAANA KALTEAIGAR
210 220
GERILTLPRG FYLKKNFTSA LLARHFLIQ
Length:229
Mass (Da):25,527
Last modified:January 23, 2007 - v3
Checksum:i32543989D710A8FD
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti58W → S in strain: ECOR 67. 1
Natural varianti60G → C in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70. 1
Natural varianti64G → R in strain: ECOR 50. 1
Natural varianti135Q → K in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64. 1
Natural varianti153Q → R in strain: ECOR 67. 1
Natural varianti155R → C in strain: ECOR 35. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00113 Genomic DNA. Translation: CAA68292.1. Sequence problems.
U16361 Genomic DNA. Translation: AAB59986.1.
U29581 Genomic DNA. Translation: AAB40478.1.
U00096 Genomic DNA. Translation: AAC75870.1.
AP009048 Genomic DNA. Translation: BAE76900.1.
AJ005726 Genomic DNA. Translation: CAB41510.1.
AJ005727 Genomic DNA. Translation: CAB41511.1.
AJ005728 Genomic DNA. Translation: CAB41512.1.
AJ005729 Genomic DNA. Translation: CAB41513.1.
AJ005730 Genomic DNA. Translation: CAB41514.1.
AJ005731 Genomic DNA. Translation: CAB41515.1.
AJ005732 Genomic DNA. Translation: CAB41516.1.
AJ005733 Genomic DNA. Translation: CAB41517.1.
AJ005734 Genomic DNA. Translation: CAB41518.1.
AJ005735 Genomic DNA. Translation: CAB41519.1.
AJ005736 Genomic DNA. Translation: CAB41520.1.
AJ005737 Genomic DNA. Translation: CAB41521.1.
AJ005738 Genomic DNA. Translation: CAB41522.1.
AJ005739 Genomic DNA. Translation: CAB41523.1.
AJ005740 Genomic DNA. Translation: CAB41524.1.
AJ005743 Genomic DNA. Translation: CAB41669.1.
AJ005744 Genomic DNA. Translation: CAB41670.1.
AJ005745 Genomic DNA. Translation: CAB41671.1.
AJ005746 Genomic DNA. Translation: CAB41696.1.
AJ005747 Genomic DNA. Translation: CAB41672.1.
AJ005748 Genomic DNA. Translation: CAB41673.1.
AJ005749 Genomic DNA. Translation: CAB41674.1.
AJ005750 Genomic DNA. Translation: CAB41675.1.
AJ005751 Genomic DNA. Translation: CAB41676.1.
AJ005752 Genomic DNA. Translation: CAB41677.1.
AJ005753 Genomic DNA. Translation: CAB41678.1.
AJ005755 Genomic DNA. Translation: CAB41679.1.
AJ238722 Genomic DNA. Translation: CAB91077.1.
AJ238719 Genomic DNA. Translation: CAB91074.1.
AJ238720 Genomic DNA. Translation: CAB91075.1.
AJ238721 Genomic DNA. Translation: CAB91076.1.
PIRiH65065. MVECMH.
RefSeqiNP_417308.1. NC_000913.3.
WP_000082201.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75870; AAC75870; b2831.
BAE76900; BAE76900; BAE76900.
GeneIDi947299.
KEGGiecj:JW2799.
eco:b2831.
PATRICi32121080. VBIEscCol129921_2929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00113 Genomic DNA. Translation: CAA68292.1. Sequence problems.
U16361 Genomic DNA. Translation: AAB59986.1.
U29581 Genomic DNA. Translation: AAB40478.1.
U00096 Genomic DNA. Translation: AAC75870.1.
AP009048 Genomic DNA. Translation: BAE76900.1.
AJ005726 Genomic DNA. Translation: CAB41510.1.
AJ005727 Genomic DNA. Translation: CAB41511.1.
AJ005728 Genomic DNA. Translation: CAB41512.1.
AJ005729 Genomic DNA. Translation: CAB41513.1.
AJ005730 Genomic DNA. Translation: CAB41514.1.
AJ005731 Genomic DNA. Translation: CAB41515.1.
AJ005732 Genomic DNA. Translation: CAB41516.1.
AJ005733 Genomic DNA. Translation: CAB41517.1.
AJ005734 Genomic DNA. Translation: CAB41518.1.
AJ005735 Genomic DNA. Translation: CAB41519.1.
AJ005736 Genomic DNA. Translation: CAB41520.1.
AJ005737 Genomic DNA. Translation: CAB41521.1.
AJ005738 Genomic DNA. Translation: CAB41522.1.
AJ005739 Genomic DNA. Translation: CAB41523.1.
AJ005740 Genomic DNA. Translation: CAB41524.1.
AJ005743 Genomic DNA. Translation: CAB41669.1.
AJ005744 Genomic DNA. Translation: CAB41670.1.
AJ005745 Genomic DNA. Translation: CAB41671.1.
AJ005746 Genomic DNA. Translation: CAB41696.1.
AJ005747 Genomic DNA. Translation: CAB41672.1.
AJ005748 Genomic DNA. Translation: CAB41673.1.
AJ005749 Genomic DNA. Translation: CAB41674.1.
AJ005750 Genomic DNA. Translation: CAB41675.1.
AJ005751 Genomic DNA. Translation: CAB41676.1.
AJ005752 Genomic DNA. Translation: CAB41677.1.
AJ005753 Genomic DNA. Translation: CAB41678.1.
AJ005755 Genomic DNA. Translation: CAB41679.1.
AJ238722 Genomic DNA. Translation: CAB91077.1.
AJ238719 Genomic DNA. Translation: CAB91074.1.
AJ238720 Genomic DNA. Translation: CAB91075.1.
AJ238721 Genomic DNA. Translation: CAB91076.1.
PIRiH65065. MVECMH.
RefSeqiNP_417308.1. NC_000913.3.
WP_000082201.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AZOX-ray1.70A1-229[»]
2AZOX-ray2.30A/B1-229[»]
ProteinModelPortaliP06722.
SMRiP06722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263275. 194 interactors.
DIPiDIP-10284N.
IntActiP06722. 10 interactors.
MINTiMINT-1542547.
STRINGi511145.b2831.

Proteomic databases

PaxDbiP06722.
PRIDEiP06722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75870; AAC75870; b2831.
BAE76900; BAE76900; BAE76900.
GeneIDi947299.
KEGGiecj:JW2799.
eco:b2831.
PATRICi32121080. VBIEscCol129921_2929.

Organism-specific databases

EchoBASEiEB0619.
EcoGeneiEG10624. mutH.

Phylogenomic databases

eggNOGiENOG4105IRT. Bacteria.
COG3066. LUCA.
KOiK03573.
OMAiVMQLRPK.

Enzyme and pathway databases

BioCyciEcoCyc:EG10624-MONOMER.
ECOL316407:JW2799-MONOMER.
MetaCyc:EG10624-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06722.
PROiP06722.

Family and domain databases

CDDicd00583. MutH_Sau3AI. 1 hit.
Gene3Di3.40.600.10. 1 hit.
HAMAPiMF_00759. MutH. 1 hit.
InterProiIPR004230. DNA_mismatch_repair_MutH.
IPR011337. DNA_rep_MutH/RE_typeII.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF02976. MutH. 1 hit.
[Graphical view]
SMARTiSM00927. MutH. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
TIGRFAMsiTIGR02248. mutH_TIGR. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMUTH_ECOLI
AccessioniPrimary (citable) accession number: P06722
Secondary accession number(s): Q2MA06
, Q9R2X2, Q9R3A8, Q9R411, Q9S6P5, Q9S6P6, Q9S6P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.