P06722 (MUTH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA mismatch repair protein mutH

Alternative name(s):
Methyl-directed mismatch repair protein

Gene names

Name:	mutH
Synonyms:	mutR, prv
Ordered Locus Names:	b2831, JW2799

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair. HAMAP MF_00759
Subcellular location	Cytoplasm HAMAP MF_00759.
Sequence similarities	Belongs to the mutH family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA modification Inferred from electronic annotation. Source: InterPro mismatch repair Inferred from direct assay. Source: EcoliWiki
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from direct assay. Source: EcoliWiki protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mutL	P23367	7	EBI-545170,EBI-554913

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 229

228

DNA mismatch repair protein mutH HAMAP MF_00759

PRO_0000198665

Natural variations

Natural variant

58

1

W → S in strain: ECOR 67.

Natural variant

60

1

G → C in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70.

Natural variant

64

1

G → R in strain: ECOR 50.

Natural variant

135

1

Q → K in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64.

Natural variant

153

1

Q → R in strain: ECOR 67.

Natural variant

155

1

R → C in strain: ECOR 35.

Experimental info

Mutagenesis

28

1

T → L in TS7; temperature-sensitive.

Mutagenesis

157

1

D → N in TS28; temperature-sensitive.

Secondary structure

1

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229

Helix Strand Turn

Details...

Helix

14 – 23

10

Turn

24 – 26

3

Helix

29 – 35

7

Turn

44 – 46

3

Helix

50 – 58

9

Helix

69 – 72

4

Beta strand

77 – 83

7

Beta strand

93 – 97

5

Beta strand

100 – 102

3

Turn

108 – 110

3

Helix

112 – 116

5

Beta strand

118 – 126

9

Helix

133 – 135

3

Beta strand

142 – 144

3

Helix

148 – 166

19

Helix

170 – 172

3

Beta strand

179 – 184

6

Beta strand

194 – 197

4

Beta strand

203 – 206

4

Beta strand

210 – 213

4

Helix

215 – 224

10

Sequences

	Sequence		Length	Mass (Da)
	P06722 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 32543989D710A8FD	FASTA	229	25,527
	10 20 30 40 50 60 MSQPRPLLSP PETEEQLLAQ AQQLSGYTLG ELAALVGLVT PENLKRDKGW IGVLLEIWLG 70 80 90 100 110 120 ASAGSKPEQD FAALGVELKT IPVDSLGRPL ETTFVCVAPL TGNSGVTWET SHVRHKLKRV 130 140 150 160 170 180 LWIPVEGERS IPLAQRRVGS PLLWSPNEEE DRQLREDWEE LMDMIVLGQV ERITARHGEY 190 200 210 220 LQIRPKAANA KALTEAIGAR GERILTLPRG FYLKKNFTSA LLARHFLIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the Escherichia coli mutH gene." Grafstrom R.H., Hoess R.H. Nucleic Acids Res. 15:3073-3084(1987) [PubMed: 3031619] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-25, MUTANTS TS7 AND TS28. Strain: K12.
[2]	Feng G., Tsui H.T., Winkler M.E., Loh T.A., Marinus M.G. Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 83; 130-131 AND 136. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	Denamur E. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 58-157. Strain: ECOR 1, ECOR 10, ECOR 13, ECOR 17, ECOR 20, ECOR 23, ECOR 24, ECOR 26, ECOR 34, ECOR 35, ECOR 37, ECOR 4, ECOR 40, ECOR 41, ECOR 45, ECOR 47, ECOR 49, ECOR 51, ECOR 52, ECOR 57, ECOR 58, ECOR 59, ECOR 62, ECOR 64, ECOR 67, ECOR 68, ECOR 70 and O2:HN / ECOR 50 / P97 / UPEC.
[6]	"Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases." Ban C., Yang W. EMBO J. 17:1526-1534(1998) [PubMed: 9482749] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y00113 Genomic DNA. Translation: CAA68292.1. Sequence problems.
U16361 Genomic DNA. Translation: AAB59986.1.
U29581 Genomic DNA. Translation: AAB40478.1.
U00096 Genomic DNA. Translation: AAC75870.1.
AP009048 Genomic DNA. Translation: BAE76900.1.
AJ005726 Genomic DNA. Translation: CAB41510.1.
AJ005727 Genomic DNA. Translation: CAB41511.1.
AJ005728 Genomic DNA. Translation: CAB41512.1.
AJ005729 Genomic DNA. Translation: CAB41513.1.
AJ005730 Genomic DNA. Translation: CAB41514.1.
AJ005731 Genomic DNA. Translation: CAB41515.1.
AJ005732 Genomic DNA. Translation: CAB41516.1.
AJ005733 Genomic DNA. Translation: CAB41517.1.
AJ005734 Genomic DNA. Translation: CAB41518.1.
AJ005735 Genomic DNA. Translation: CAB41519.1.
AJ005736 Genomic DNA. Translation: CAB41520.1.
AJ005737 Genomic DNA. Translation: CAB41521.1.
AJ005738 Genomic DNA. Translation: CAB41522.1.
AJ005739 Genomic DNA. Translation: CAB41523.1.
AJ005740 Genomic DNA. Translation: CAB41524.1.
AJ005743 Genomic DNA. Translation: CAB41669.1.
AJ005744 Genomic DNA. Translation: CAB41670.1.
AJ005745 Genomic DNA. Translation: CAB41671.1.
AJ005746 Genomic DNA. Translation: CAB41696.1.
AJ005747 Genomic DNA. Translation: CAB41672.1.
AJ005748 Genomic DNA. Translation: CAB41673.1.
AJ005749 Genomic DNA. Translation: CAB41674.1.
AJ005750 Genomic DNA. Translation: CAB41675.1.
AJ005751 Genomic DNA. Translation: CAB41676.1.
AJ005752 Genomic DNA. Translation: CAB41677.1.
AJ005753 Genomic DNA. Translation: CAB41678.1.
AJ005755 Genomic DNA. Translation: CAB41679.1.
AJ238722 Genomic DNA. Translation: CAB91077.1.
AJ238719 Genomic DNA. Translation: CAB91074.1.
AJ238720 Genomic DNA. Translation: CAB91075.1.
AJ238721 Genomic DNA. Translation: CAB91076.1.

PIR

MVECMH. H65065.

RefSeq

NP_417308.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AZO	X-ray	1.70	A	1-229	[»]
2AZO	X-ray	2.30	A/B	1-229	[»]

ProteinModelPortal

P06722.

SMR

P06722. Positions 3-229.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10284N.

IntAct

P06722. 10 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000001751; EBESCP00000001751; EBESCG00000001443.
EBESCT00000016719; EBESCP00000016010; EBESCG00000015778.

GeneID

947299.

GenomeReviews

Gene locus JW2799 in contig AP009048_GR.
Gene locus b2831 in contig U00096_GR.

KEGG

ecj:JW2799.
eco:b2831.

PATRIC

32121080. VBIEscCol129921_2929.

Organism-specific databases

EchoBASE

EB0619.

EcoGene

EG10624. mutH.

Phylogenomic databases

eggNOG

COG3066.

GeneTree

EBGT00050000011710.

OMA

QDWEELM.

ProtClustDB

PRK05070.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10624-MONOMER.
MetaCyc:EG10624-MONOMER.

Gene expression databases

Genevestigator

P06722.

Family and domain databases

HAMAP

MF_00759. MutH.
[Tree]

InterPro

IPR004230. DNA_mismatch_repair_MutH.
IPR011337. DNA_rep_MutH/RE_typeII.
IPR011335. Restrct_endonuc_II-like.
[Graphical view]

Gene3D

G3DSA:3.40.600.10. Restrict_endonuc_II/DNA_repair. 1 hit.

KO

K03573.

Pfam

PF02976. MutH. 1 hit.
[Graphical view]

SMART

SM00927. MutH. 1 hit.
[Graphical view]

SUPFAM

SSF52980. Restrict_endonuc_II-like_core. 1 hit.

TIGRFAMs

TIGR02248. MutH_TIGR. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MUTH_ECOLI

Accession

Primary (citable) accession number: P06722
Secondary accession number(s): Q2MA06 , Q9R2X2, Q9R3A8, Q9R411, Q9S6P5, Q9S6P6, Q9S6P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program