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Protein

Cystathionine beta-lyase MetC

Gene

metC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.

Cofactori

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from L-cystathionine.
Proteins known to be involved in this subpathway in this organism are:
  1. Cystathionine beta-lyase MetC (metC)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from L-cystathionine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • cystathionine beta-lyase activity Source: EcoCyc
  • L-cysteine desulfhydrase activity Source: EcoCyc
  • pyridoxal phosphate binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:CYSTATHIONINE-BETA-LYASE-MONOMER.
ECOL316407:JW2975-MONOMER.
MetaCyc:CYSTATHIONINE-BETA-LYASE-MONOMER.
BRENDAi4.4.1.8. 2026.
UniPathwayiUPA00051; UER00078.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine beta-lyase MetC (EC:4.4.1.8)
Short name:
CBL
Alternative name(s):
Beta-cystathionase
Cysteine lyase
Gene namesi
Name:metC
Ordered Locus Names:b3008, JW2975
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10583. metC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075079.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001147681 – 395Cystathionine beta-lyase MetCAdd BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei210N6-(pyridoxal phosphate)lysine1 Publication1

Proteomic databases

PaxDbiP06721.
PRIDEiP06721.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi4261419. 33 interactors.
DIPiDIP-10193N.
IntActiP06721. 6 interactors.
STRINGi511145.b3008.

Chemistry databases

BindingDBiP06721.

Structurei

Secondary structure

1395
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 12Combined sources7
Helixi17 – 20Combined sources4
Beta strandi23 – 25Combined sources3
Beta strandi32 – 34Combined sources3
Helixi39 – 47Combined sources9
Turni48 – 52Combined sources5
Helixi57 – 59Combined sources3
Helixi62 – 75Combined sources14
Beta strandi78 – 85Combined sources8
Helixi86 – 97Combined sources12
Beta strandi103 – 107Combined sources5
Helixi112 – 120Combined sources9
Helixi122 – 125Combined sources4
Beta strandi128 – 132Combined sources5
Helixi137 – 143Combined sources7
Beta strandi148 – 156Combined sources9
Turni158 – 160Combined sources3
Helixi166 – 176Combined sources11
Beta strandi181 – 185Combined sources5
Turni187 – 192Combined sources6
Helixi196 – 199Combined sources4
Beta strandi202 – 207Combined sources6
Turni208 – 213Combined sources6
Beta strandi215 – 217Combined sources3
Beta strandi221 – 225Combined sources5
Turni227 – 229Combined sources3
Helixi230 – 239Combined sources10
Helixi246 – 256Combined sources11
Helixi259 – 278Combined sources20
Beta strandi283 – 287Combined sources5
Helixi297 – 303Combined sources7
Beta strandi309 – 317Combined sources9
Helixi321 – 328Combined sources8
Beta strandi342 – 344Combined sources3
Beta strandi346 – 350Combined sources5
Helixi352 – 356Combined sources5
Beta strandi370 – 374Combined sources5
Helixi380 – 392Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CL1X-ray1.83A/B1-395[»]
1CL2X-ray2.20A/B1-395[»]
2FQ6X-ray1.78A/B1-395[»]
2GQNX-ray1.80A/B1-395[»]
4ITGX-ray1.74A/B1-395[»]
4ITXX-ray1.61A/B1-395[»]
ProteinModelPortaliP06721.
SMRiP06721.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06721.

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

eggNOGiENOG4105C28. Bacteria.
COG0626. LUCA.
HOGENOMiHOG000246416.
InParanoidiP06721.
KOiK01760.
OMAiPTHFAFQ.
PhylomeDBiP06721.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR006233. Cys_b_lyase_bac.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01324. cysta_beta_ly_B. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06721-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKKLDTQL VNAGRSKKYT LGAVNSVIQR ASSLVFDSVE AKKHATRNRA
60 70 80 90 100
NGELFYGRRG TLTHFSLQQA MCELEGGAGC VLFPCGAAAV ANSILAFIEQ
110 120 130 140 150
GDHVLMTNTA YEPSQDFCSK ILSKLGVTTS WFDPLIGADI VKHLQPNTKI
160 170 180 190 200
VFLESPGSIT MEVHDVPAIV AAVRSVVPDA IIMIDNTWAA GVLFKALDFG
210 220 230 240 250
IDVSIQAATK YLVGHSDAMI GTAVCNARCW EQLRENAYLM GQMVDADTAY
260 270 280 290 300
ITSRGLRTLG VRLRQHHESS LKVAEWLAEH PQVARVNHPA LPGSKGHEFW
310 320 330 340 350
KRDFTGSSGL FSFVLKKKLN NEELANYLDN FSLFSMAYSW GGYESLILAN
360 370 380 390
QPEHIAAIRP QGEIDFSGTL IRLHIGLEDV DDLIADLDAG FARIV
Length:395
Mass (Da):43,212
Last modified:January 1, 1988 - v1
Checksum:iFD3308423D6427C6
GO

Sequence cautioni

The sequence AAA69175 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12858 Genomic DNA. Translation: AAA24158.1.
U28377 Genomic DNA. Translation: AAA69175.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76044.1.
AP009048 Genomic DNA. Translation: BAE77065.1.
PIRiA25153. WZECCB.
RefSeqiNP_417481.1. NC_000913.3.
WP_001301079.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76044; AAC76044; b3008.
BAE77065; BAE77065; BAE77065.
GeneIDi946240.
KEGGiecj:JW2975.
eco:b3008.
PATRICi32121430. VBIEscCol129921_3102.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12858 Genomic DNA. Translation: AAA24158.1.
U28377 Genomic DNA. Translation: AAA69175.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76044.1.
AP009048 Genomic DNA. Translation: BAE77065.1.
PIRiA25153. WZECCB.
RefSeqiNP_417481.1. NC_000913.3.
WP_001301079.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CL1X-ray1.83A/B1-395[»]
1CL2X-ray2.20A/B1-395[»]
2FQ6X-ray1.78A/B1-395[»]
2GQNX-ray1.80A/B1-395[»]
4ITGX-ray1.74A/B1-395[»]
4ITXX-ray1.61A/B1-395[»]
ProteinModelPortaliP06721.
SMRiP06721.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261419. 33 interactors.
DIPiDIP-10193N.
IntActiP06721. 6 interactors.
STRINGi511145.b3008.

Chemistry databases

BindingDBiP06721.
ChEMBLiCHEMBL1075079.

Proteomic databases

PaxDbiP06721.
PRIDEiP06721.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76044; AAC76044; b3008.
BAE77065; BAE77065; BAE77065.
GeneIDi946240.
KEGGiecj:JW2975.
eco:b3008.
PATRICi32121430. VBIEscCol129921_3102.

Organism-specific databases

EchoBASEiEB0578.
EcoGeneiEG10583. metC.

Phylogenomic databases

eggNOGiENOG4105C28. Bacteria.
COG0626. LUCA.
HOGENOMiHOG000246416.
InParanoidiP06721.
KOiK01760.
OMAiPTHFAFQ.
PhylomeDBiP06721.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00078.
BioCyciEcoCyc:CYSTATHIONINE-BETA-LYASE-MONOMER.
ECOL316407:JW2975-MONOMER.
MetaCyc:CYSTATHIONINE-BETA-LYASE-MONOMER.
BRENDAi4.4.1.8. 2026.

Miscellaneous databases

EvolutionaryTraceiP06721.
PROiP06721.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR006233. Cys_b_lyase_bac.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01324. cysta_beta_ly_B. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMETC_ECOLI
AccessioniPrimary (citable) accession number: P06721
Secondary accession number(s): Q2M9J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.