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Protein

Alpha-galactosidase

Gene

melA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei151SubstrateBy similarity1
Metal bindingi173ManganeseBy similarity1
Active sitei174Proton donorBy similarity1
Metal bindingi203ManganeseBy similarity1
Binding sitei287SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi5 – 71NADBy similarityAdd BLAST67

GO - Molecular functioni

  • alpha-galactosidase activity Source: EcoCyc
  • manganese ion binding Source: EcoCyc
  • NAD+ binding Source: EcoCyc
  • oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
  • raffinose alpha-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  • melibiose catabolic process Source: EcoCyc

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandManganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciEcoCyc:ALPHAGALACTOSID-MONOMER
MetaCyc:ALPHAGALACTOSID-MONOMER

Protein family/group databases

CAZyiGH4 Glycoside Hydrolase Family 4

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Melibiase
Gene namesi
Name:melA
Synonyms:mel-7
Ordered Locus Names:b4119, JW4080
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10577 melA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3696

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001698521 – 451Alpha-galactosidaseAdd BLAST451

Proteomic databases

PaxDbiP06720
PRIDEiP06720

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4262685, 8 interactors
DIPiDIP-10179N
IntActiP06720, 3 interactors
STRINGi316385.ECDH10B_4311

Structurei

3D structure databases

ProteinModelPortaliP06720
SMRiP06720
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 4 family.Curated

Phylogenomic databases

eggNOGiENOG4105F8D Bacteria
COG1486 LUCA
HOGENOMiHOG000239812
InParanoidiP06720
KOiK07406
OMAiLQYVNPM
PhylomeDBiP06720

Family and domain databases

InterProiView protein in InterPro
IPR019802 GlycHydrolase_4_CS
IPR001088 Glyco_hydro_4
IPR022616 Glyco_hydro_4_C
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR32092 PTHR32092, 1 hit
PfamiView protein in Pfam
PF02056 Glyco_hydro_4, 1 hit
PF11975 Glyco_hydro_4C, 1 hit
PRINTSiPR00732 GLHYDRLASE4
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
PROSITEiView protein in PROSITE
PS01324 GLYCOSYL_HYDROL_F4, 1 hit

Sequencei

Sequence statusi: Complete.

P06720-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSAPKITFI GAGSTIFVKN ILGDVFHREA LKTAHIALMD IDPTRLEESH
60 70 80 90 100
IVVRKLMDSA GASGKITCHT QQKEALEDAD FVVVAFQIGG YEPCTVTDFE
110 120 130 140 150
VCKRHGLEQT IADTLGPGGI MRALRTIPHL WQICEDMTEV CPDATMLNYV
160 170 180 190 200
NPMAMNTWAM YARYPHIKQV GLCHSVQGTA EELARDLNID PATLRYRCAG
210 220 230 240 250
INHMAFYLEL ERKTADGSYV NLYPELLAAY EAGQAPKPNI HGNTRCQNIV
260 270 280 290 300
RYEMFKKLGY FVTESSEHFA EYTPWFIKPG REDLIERYKV PLDEYPKRCV
310 320 330 340 350
EQLANWHKEL EEYKKASRID IKPSREYAST IMNAIWTGEP SVIYGNVRND
360 370 380 390 400
GLIDNLPQGC CVEVACLVDA NGIQPTKVGT LPSHLAALMQ TNINVQTLLT
410 420 430 440 450
EAILTENRDR VYHAAMMDPH TAAVLGIDEI YALVDDLIAA HGDWLPGWLH

R
Length:451
Mass (Da):50,657
Last modified:January 1, 1988 - v1
Checksum:iCB20367C0860AB5F
GO

Sequence cautioni

The sequence AAA24149 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04894 Genomic DNA Translation: CAA28581.1
K01490 Genomic DNA Translation: AAA24149.1 Different initiation.
U14003 Genomic DNA Translation: AAA97019.1
U00096 Genomic DNA Translation: AAC77080.1
AP009048 Genomic DNA Translation: BAE78121.1
PIRiA26571 GBECAG
RefSeqiNP_418543.1, NC_000913.3
WP_000986601.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77080; AAC77080; b4119
BAE78121; BAE78121; BAE78121
GeneIDi948636
KEGGiecj:JW4080
eco:b4119
PATRICifig|1411691.4.peg.2581

Similar proteinsi

Entry informationi

Entry nameiAGAL_ECOLI
AccessioniPrimary (citable) accession number: P06720
Secondary accession number(s): Q2M6I5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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