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Protein

Alpha-galactosidase

Gene

melA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei151SubstrateBy similarity1
Metal bindingi173ManganeseBy similarity1
Active sitei174Proton donorBy similarity1
Metal bindingi203ManganeseBy similarity1
Binding sitei287SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi5 – 71NADBy similarityAdd BLAST67

GO - Molecular functioni

  • alpha-galactosidase activity Source: EcoCyc
  • manganese ion binding Source: EcoCyc
  • NAD+ binding Source: EcoCyc
  • oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
  • raffinose alpha-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  • melibiose catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Manganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciEcoCyc:ALPHAGALACTOSID-MONOMER.
ECOL316407:JW4080-MONOMER.
MetaCyc:ALPHAGALACTOSID-MONOMER.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Melibiase
Gene namesi
Name:melA
Synonyms:mel-7
Ordered Locus Names:b4119, JW4080
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10577. melA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3696.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001698521 – 451Alpha-galactosidaseAdd BLAST451

Proteomic databases

PaxDbiP06720.
PRIDEiP06720.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4262685. 7 interactors.
DIPiDIP-10179N.
IntActiP06720. 3 interactors.
STRINGi511145.b4119.

Structurei

3D structure databases

ProteinModelPortaliP06720.
SMRiP06720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 4 family.Curated

Phylogenomic databases

eggNOGiENOG4105F8D. Bacteria.
COG1486. LUCA.
HOGENOMiHOG000239812.
InParanoidiP06720.
KOiK07406.
OMAiNLPKEAC.
PhylomeDBiP06720.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06720-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSAPKITFI GAGSTIFVKN ILGDVFHREA LKTAHIALMD IDPTRLEESH
60 70 80 90 100
IVVRKLMDSA GASGKITCHT QQKEALEDAD FVVVAFQIGG YEPCTVTDFE
110 120 130 140 150
VCKRHGLEQT IADTLGPGGI MRALRTIPHL WQICEDMTEV CPDATMLNYV
160 170 180 190 200
NPMAMNTWAM YARYPHIKQV GLCHSVQGTA EELARDLNID PATLRYRCAG
210 220 230 240 250
INHMAFYLEL ERKTADGSYV NLYPELLAAY EAGQAPKPNI HGNTRCQNIV
260 270 280 290 300
RYEMFKKLGY FVTESSEHFA EYTPWFIKPG REDLIERYKV PLDEYPKRCV
310 320 330 340 350
EQLANWHKEL EEYKKASRID IKPSREYAST IMNAIWTGEP SVIYGNVRND
360 370 380 390 400
GLIDNLPQGC CVEVACLVDA NGIQPTKVGT LPSHLAALMQ TNINVQTLLT
410 420 430 440 450
EAILTENRDR VYHAAMMDPH TAAVLGIDEI YALVDDLIAA HGDWLPGWLH

R
Length:451
Mass (Da):50,657
Last modified:January 1, 1988 - v1
Checksum:iCB20367C0860AB5F
GO

Sequence cautioni

The sequence AAA24149 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04894 Genomic DNA. Translation: CAA28581.1.
K01490 Genomic DNA. Translation: AAA24149.1. Different initiation.
U14003 Genomic DNA. Translation: AAA97019.1.
U00096 Genomic DNA. Translation: AAC77080.1.
AP009048 Genomic DNA. Translation: BAE78121.1.
PIRiA26571. GBECAG.
RefSeqiNP_418543.1. NC_000913.3.
WP_000986601.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77080; AAC77080; b4119.
BAE78121; BAE78121; BAE78121.
GeneIDi948636.
KEGGiecj:JW4080.
eco:b4119.
PATRICi32123803. VBIEscCol129921_4250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04894 Genomic DNA. Translation: CAA28581.1.
K01490 Genomic DNA. Translation: AAA24149.1. Different initiation.
U14003 Genomic DNA. Translation: AAA97019.1.
U00096 Genomic DNA. Translation: AAC77080.1.
AP009048 Genomic DNA. Translation: BAE78121.1.
PIRiA26571. GBECAG.
RefSeqiNP_418543.1. NC_000913.3.
WP_000986601.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP06720.
SMRiP06720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262685. 7 interactors.
DIPiDIP-10179N.
IntActiP06720. 3 interactors.
STRINGi511145.b4119.

Chemistry databases

ChEMBLiCHEMBL3696.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Proteomic databases

PaxDbiP06720.
PRIDEiP06720.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77080; AAC77080; b4119.
BAE78121; BAE78121; BAE78121.
GeneIDi948636.
KEGGiecj:JW4080.
eco:b4119.
PATRICi32123803. VBIEscCol129921_4250.

Organism-specific databases

EchoBASEiEB0572.
EcoGeneiEG10577. melA.

Phylogenomic databases

eggNOGiENOG4105F8D. Bacteria.
COG1486. LUCA.
HOGENOMiHOG000239812.
InParanoidiP06720.
KOiK07406.
OMAiNLPKEAC.
PhylomeDBiP06720.

Enzyme and pathway databases

BioCyciEcoCyc:ALPHAGALACTOSID-MONOMER.
ECOL316407:JW4080-MONOMER.
MetaCyc:ALPHAGALACTOSID-MONOMER.

Miscellaneous databases

PROiP06720.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGAL_ECOLI
AccessioniPrimary (citable) accession number: P06720
Secondary accession number(s): Q2M6I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.