P06720 (AGAL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-galactosidase
EC=3.2.1.22

Alternative name(s):
Melibiase

Gene names

Name:	melA
Synonyms:	mel-7
Ordered Locus Names:	b4119, JW4080

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Cofactor	Binds 1 NAD per subunit. Ref.6 Binds 1 manganese ion per subunit. Ref.6
Subunit structure	Homodimer. Ref.6
Sequence similarities	Belongs to the glycosyl hydrolase 4 family.
Sequence caution	The sequence AAA24149.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	Manganese Metal-binding NAD
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Alpha-galactosidase		PRO_0000169852
Regions
	Nucleotide binding	5 – 71	67	NAD By similarity
Sites
	Active site	174	1	Proton donor By similarity
	Metal binding	173	1	Manganese By similarity
	Metal binding	203	1	Manganese By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	287	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06720 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: CB20367C0860AB5F	FASTA	451	50,657
	10 20 30 40 50 60 MMSAPKITFI GAGSTIFVKN ILGDVFHREA LKTAHIALMD IDPTRLEESH IVVRKLMDSA 70 80 90 100 110 120 GASGKITCHT QQKEALEDAD FVVVAFQIGG YEPCTVTDFE VCKRHGLEQT IADTLGPGGI 130 140 150 160 170 180 MRALRTIPHL WQICEDMTEV CPDATMLNYV NPMAMNTWAM YARYPHIKQV GLCHSVQGTA 190 200 210 220 230 240 EELARDLNID PATLRYRCAG INHMAFYLEL ERKTADGSYV NLYPELLAAY EAGQAPKPNI 250 260 270 280 290 300 HGNTRCQNIV RYEMFKKLGY FVTESSEHFA EYTPWFIKPG REDLIERYKV PLDEYPKRCV 310 320 330 340 350 360 EQLANWHKEL EEYKKASRID IKPSREYAST IMNAIWTGEP SVIYGNVRND GLIDNLPQGC 370 380 390 400 410 420 CVEVACLVDA NGIQPTKVGT LPSHLAALMQ TNINVQTLLT EAILTENRDR VYHAAMMDPH 430 440 450 TAAVLGIDEI YALVDDLIAA HGDWLPGWLH R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the melA gene, coding for alpha-galactosidase in Escherichia coli K-12." Liljestroem P.L., Liljestroem P. Nucleic Acids Res. 15:2213-2220(1987) [PubMed: 3031590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Nucleotide sequence of the promoter region of the melibiose operon of Escherichia coli." Shimamoto T., Yazyu H., Futai M., Tsuchiya T. Biochem. Biophys. Res. Commun. 121:41-46(1984) [PubMed: 6329200] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52.
[6]	"Purification and analysis of the structure of alpha-galactosidase from Escherichia coli." Nagao Y., Nakada T., Imoto M., Shimamoto T., Sakai S., Tsuda M., Tsuchiya T. Biochem. Biophys. Res. Commun. 151:236-241(1988) [PubMed: 2831880] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20, COFACTOR, SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04894 Genomic DNA. Translation: CAA28581.1. K01490 Genomic DNA. Translation: AAA24149.1. Different initiation. U14003 Genomic DNA. Translation: AAA97019.1. U00096 Genomic DNA. Translation: AAC77080.1. AP009048 Genomic DNA. Translation: BAE78121.1.
PIR	GBECAG. A26571.
RefSeq	NP_418543.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P06720.
SMR	P06720. Positions 2-438.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10179N.
IntAct	P06720. 3 interactions.
Protein family/group databases
CAZy	GH4. Glycoside Hydrolase Family 4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003260; EBESCP00000003260; EBESCG00000002675. EBESCT00000017118; EBESCP00000016409; EBESCG00000016177.
GeneID	948636.
GenomeReviews	Gene locus JW4080 in contig AP009048_GR. Gene locus b4119 in contig U00096_GR.
KEGG	ecj:JW4080. eco:b4119.
PATRIC	32123803. VBIEscCol129921_4250.
Organism-specific databases
EchoBASE	EB0572.
EcoGene	EG10577. melA.
Phylogenomic databases
eggNOG	COG1486.
GeneTree	EBGT00050000010199.
HOGENOM	HBG511715.
OMA	MAINTWA.
PhylomeDB	P06720.
ProtClustDB	PRK15076.
Enzyme and pathway databases
BioCyc	EcoCyc:ALPHAGALACTOSID-MONOMER. MetaCyc:ALPHAGALACTOSID-MONOMER.
Gene expression databases
Genevestigator	P06720.
Family and domain databases
InterPro	IPR019802. GlycHydrolase_4_CS. IPR001088. Glyco_hydro_4. IPR022616. Glyco_hydro_4_C. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K07406.
Pfam	PF02056. Glyco_hydro_4. 1 hit. PF11975. Glyco_hydro_4C. 1 hit. [Graphical view]
PRINTS	PR00732. GLHYDRLASE4.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
PROSITE	PS01324. GLYCOSYL_HYDROL_F4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAL_ECOLI

Accession

Primary (citable) accession number: P06720
Secondary accession number(s): Q2M6I5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	January 25, 2012
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program