true1988-01-012023-06-28108ELAP_ECOLXEvolutionary origin of pathogenic determinants in enterotoxigenic Escherichia coli and Vibrio cholerae O1.Yamamoto T.Gojobori T.Yokota T.doi:10.1128/jb.169.3.1352-1357.19871987J. Bacteriol.1691352-1357NUCLEOTIDE SEQUENCE [GENOMIC DNA]Isolate P307 / ETECIsolate PCG86 / ETECA comparison of the nucleotide sequence of the A subunit of heat-labile enterotoxin and cholera toxin.Dykes C.W.Halliday I.J.Hobden A.N.Read M.J.Harford S.1985FEMS Microbiol. Lett.26171-174NUCLEOTIDE SEQUENCE [GENOMIC DNA]Escherichia coli heat-labile enterotoxin. Nucleotide sequence of the A subunit gene.Spicer E.K.Noble J.A.doi:10.1016/s0021-9258(19)83837-11982J. Biol. Chem.2575716-5721NUCLEOTIDE SEQUENCE [GENOMIC DNA]A single amino acid substitution in the A subunit of Escherichia coli enterotoxin results in a loss of its toxic activity.Tsuji T.Inoue T.Miyama A.Okamoto K.Honda T.Miwatani T.doi:10.1016/s0021-9258(18)45736-51990J. Biol. Chem.26522520-22525NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-258Trachman J.D.Maas W.K.1991-07EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40Crystal structure of a cholera toxin-related heat-labile enterotoxin from E. coli.Sixma T.K.Pronk S.E.Kalk K.H.Wartna E.S.van Zanten B.A.M.Witholt B.Hol W.G.J.doi:10.1038/351371a01991Nature351371-377X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)Refined structure of Escherichia coli heat-labile enterotoxin, a close relative of cholera toxin.Sixma T.K.van Zanten B.A.M.Dauter Z.Hol W.G.J.doi:10.1006/jmbi.1993.12091993J. Mol. Biol.230890-918X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS)Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis.Pizza M.Domenighini M.Hol W.G.J.Giannelli V.Fontana M.R.Giuliani M.M.Magagnoli C.Peppoloni S.Manetti R.Rappuoli R.doi:10.1111/j.1365-2958.1994.tb01266.x1994Mol. Microbiol.1451-60X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 19-258MUTAGENESIS OF ARG-25; VAL-71; ARG-72; TYR-77; SER-81; ALA-90; VAL-115; TYR-122; HIS-125; GLU-128; GLU-130; SER-132 AND ARG-210Identification of errors among database sequence entries and comparison of correct amino acid sequences for the heat-labile enterotoxins of Escherichia coli and Vibrio cholerae.Domenighini M.Pizza M.Jobling M.G.Holmes R.K.Rappuoli R.doi:10.1111/j.1365-2958.1995.tb02289.x1995Mol. Microbiol.151165-1167DISCUSSION OF SEQUENCEQLECA2.50A=19-209C=210-2582.00A=19-2582.00A=19-2512.20A=19-206C=210-2582.60A=22-206C=210-2542.40A=19-209C=210-2582.13A=19-210C=211-2581.95A=22-206C=214-2542.30A=22-206C=214-2541Heat-Labile Enterotoxin, subunit AEnterotoxin_AEnterotoxin_aENTEROTOXINAADP-ribosylationHeat-labile enterotoxin A chainLT-A, porcineLTP-AeltAltpAThe biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.Heterohexamer of one A chain and of five B chains.Belongs to the enterotoxin A family.118Heat-labile enterotoxin A chain27818192581302539205217In inactive mutant.KAbolishes toxicity.KAbolishes toxicity.DE71No effect.AK72No effect.M77Abolishes toxicity.K81No effect.EHR90Abolishes toxicity.K115Abolishes toxicity.DK122Strongly reduces toxicity.E125Abolishes toxicity.S128Abolishes toxicity.SAbolishes toxicity.EK132No effect.N210FRS3745Y93LTIYIVIA100110IS119120G159D207232931474951535964708384949597991061121141261341391411421491521651701761791801821901931972002152402412442502531988-01-011true29902ee43d5e2e8d7a8fc86a9bc24c8fccc8dMKNITFIFFILLASPLYANGDRLYRADSRPPDEIKRSGGLMPRGHNEYFDRGTQMNINLYDHARGTQTGFVRYDDGYVSTSLSLRSAHLAGQSILSGYSTYYIYVIATAPNMFNVNDVLGVYSPHPYEQEVSALGGIPYSQIYGWYRVNFGVIDERLHRNREYRDRYYRNLNIAPAEDGYRLAGFPPDHQAWREEPWIHHAPQGCGNSSRTITGDTCNEETQNLSTIYLREYQSKVKRQIFSDYQSEVDIYNRIRDELtruetrue