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Protein

Colicin-Ia

Gene

cia

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin

Protein family/group databases

TCDBi1.C.1.1.1. the channel-forming colicin (colicin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-Ia
Gene namesi
Name:cia
Encoded oniPlasmid ColIa-CA532 Publications
Plasmid ColIa-EC281 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei580 – 59415HelicalAdd
BLAST
Transmembranei597 – 61216HelicalAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 626626Colicin-IaPRO_0000218675Add
BLAST

Proteomic databases

PRIDEiP06716.

Interactioni

Protein-protein interaction databases

IntActiP06716. 1 interaction.
MINTiMINT-4794283.

Structurei

Secondary structure

1
626
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi48 – 514Combined sources
Helixi67 – 12155Combined sources
Helixi126 – 16843Combined sources
Beta strandi172 – 1743Combined sources
Helixi176 – 282107Combined sources
Helixi285 – 2917Combined sources
Turni293 – 2953Combined sources
Beta strandi298 – 30811Combined sources
Turni312 – 3143Combined sources
Beta strandi316 – 32611Combined sources
Helixi329 – 3379Combined sources
Helixi340 – 3467Combined sources
Helixi359 – 38325Combined sources
Helixi470 – 48011Combined sources
Turni481 – 4844Combined sources
Helixi490 – 49910Combined sources
Helixi501 – 5044Combined sources
Helixi511 – 52111Combined sources
Helixi525 – 53915Combined sources
Helixi548 – 56013Combined sources
Helixi565 – 5717Combined sources
Turni572 – 5743Combined sources
Helixi580 – 59112Combined sources
Helixi597 – 61216Combined sources
Helixi614 – 6218Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CIIX-ray3.00A23-624[»]
2HDIX-ray2.50B282-385[»]
ProteinModelPortaliP06716.
SMRiP06716. Positions 23-624.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06716.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 225203Translocation (T)Add
BLAST
Regioni282 – 385104Receptor-binding (R)Add
BLAST
Regioni450 – 626177Channel (C)Add
BLAST

Sequence similaritiesi

Belongs to the channel forming colicin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
1.20.250.10. 1 hit.
3.30.305.10. 1 hit.
InterProiIPR000293. Channel_colicin_C.
IPR014740. Channel_colicin_cen.
IPR014739. Channel_colicin_N.
[Graphical view]
PfamiPF01024. Colicin. 1 hit.
PF11504. Colicin_Ia. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06716-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDPVRITNP GAESLGYDSD GHEIMAVDIY VNPPRVDVFH GTPPAWSSFG
60 70 80 90 100
NKTIWGGNEW VDDSPTRSDI EKRDKEITAY KNTLSAQQKE NENKRTEAGK
110 120 130 140 150
RLSAAIAARE KDENTLKTLR AGNADAADIT RQEFRLLQAE LREYGFRTEI
160 170 180 190 200
AGYDALRLHT ESRMLFADAD SLRISPREAR SLIEQAEKRQ KDAQNADKKA
210 220 230 240 250
ADMLAEYERR KGILDTRLSE LEKNGGAALA VLDAQQARLL GQQTRNDRAI
260 270 280 290 300
SEARNKLSSV TESLNTARNA LTRAEQQLTQ QKNTPDGKTI VSPEKFPGRS
310 320 330 340 350
STNHSIVVSG DPRFAGTIKI TTSAVIDNRA NLNYLLSHSG LDYKRNILND
360 370 380 390 400
RNPVVTEDVE GDKKIYNAEV AEWDKLRQRL LDARNKITSA ESAVNSARNN
410 420 430 440 450
LSARTNEQKH ANDALNALLK EKENIRNQLS GINQKIAEEK RKQDELKATK
460 470 480 490 500
DAINFTTEFL KSVSEKYGAK AEQLAREMAG QAKGKKIRNV EEALKTYEKY
510 520 530 540 550
RADINKKINA KDRAAIAAAL ESVKLSDISS NLNRFSRGLG YAGKFTSLAD
560 570 580 590 600
WITEFGKAVR TENWRPLFVK TETIIAGNAA TALVALVFSI LTGSALGIIG
610 620
YGLLMAVTGA LIDESLVEKA NKFWGI
Length:626
Mass (Da):69,429
Last modified:October 24, 2003 - v2
Checksum:i3DC0DF322F405D39
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti304 – 3041H → D in AAA59396 (PubMed:7972047).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13819 Genomic DNA. Translation: AAA23182.2.
U15622 Genomic DNA. Translation: AAA59396.1.
PIRiC25035.
RefSeqiWP_001582575.1. NZ_LVOL01000108.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13819 Genomic DNA. Translation: AAA23182.2.
U15622 Genomic DNA. Translation: AAA59396.1.
PIRiC25035.
RefSeqiWP_001582575.1. NZ_LVOL01000108.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CIIX-ray3.00A23-624[»]
2HDIX-ray2.50B282-385[»]
ProteinModelPortaliP06716.
SMRiP06716. Positions 23-624.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP06716. 1 interaction.
MINTiMINT-4794283.

Protein family/group databases

TCDBi1.C.1.1.1. the channel-forming colicin (colicin) family.

Proteomic databases

PRIDEiP06716.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP06716.

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
1.20.250.10. 1 hit.
3.30.305.10. 1 hit.
InterProiIPR000293. Channel_colicin_C.
IPR014740. Channel_colicin_cen.
IPR014739. Channel_colicin_N.
[Graphical view]
PfamiPF01024. Colicin. 1 hit.
PF11504. Colicin_Ia. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "DNA and amino acid sequence analysis of structural and immunity genes of colicins Ia and Ib."
    Mankovich J.A., Hsu C.-H., Konisky J.
    J. Bacteriol. 168:228-236(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: ColIa-CA53
  2. Konisky J.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 304.
  3. "Nucleotide polymorphism in colicin E1 and Ia plasmids from natural isolates of Escherichia coli."
    Riley M.A., Tan Y., Wang J.
    Proc. Natl. Acad. Sci. U.S.A. 91:11276-11280(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: ColIa-EC28
  4. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 23-624.
    Plasmid: ColIa-CA53

Entry informationi

Entry nameiCEIA_ECOLX
AccessioniPrimary (citable) accession number: P06716
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 24, 2003
Last modified: July 6, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.