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Protein

Cell division control protein 31

Gene

CDC31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC) and the spindle pole body (SPB) half-bridge. At the SPB, it is recruited by KAR1 and MPS3 to the SPB half-bridge and involved in the initial steps of SPB duplication. It probably plays a similar role in de novo assembly of NPCs at the nuclear envelope. Also involved in connection with the protein kinase KIC1 in the maintenance of cell morphology and integrity.6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi33 – 44121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi142 – 153122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • identical protein binding Source: IntAct
  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • microtubule nucleation Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • mRNA transport Source: UniProtKB-KW
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • protein transport Source: UniProtKB-KW
  • regulation of kinase activity Source: SGD
  • spindle pole body duplication Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, mRNA transport, Protein transport, Translocation, Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33748-MONOMER.
ReactomeiR-SCE-5696394. DNA Damage Recognition in GG-NER.
R-SCE-5696395. Formation of Incision Complex in GG-NER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 31
Alternative name(s):
Nuclear pore protein CDC31
Nucleoporin CDC31
Gene namesi
Name:CDC31
Synonyms:DSK1
Ordered Locus Names:YOR257W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR257W.
SGDiS000005783. CDC31.

Subcellular locationi

GO - Cellular componenti

  • half bridge of spindle pole body Source: SGD
  • nuclear pore Source: UniProtKB-SubCell
  • transcription export complex 2 Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Cell division control protein 31PRO_0000073577Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06704.

PTM databases

iPTMnetiP06704.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. The spindle pole body (SPB), acting as the connector of microtubule arrays in the cytoplasm and the nucleoplasm, is involved in nuclear positioning before chromosome segregation, SPB separation, spindle formation, chromosome segregation, nuclear migration into the bud, nuclear reorientation after cytokinesis and nuclear fusion during conjugation. The SPB half-bridge, which is tightly associated with the cytoplasmic side of the nuclear envelope and the SPB, is playing a key role as the starting structure for and in the initiation of SPB duplication in G1. At the SPB half-bridge CDC31 interacts with KAR1, MPS3 and SFI1. It also interacts with KIC1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-4259,EBI-4259
MPS3P470692EBI-4259,EBI-25811
SAC3P4667410EBI-4259,EBI-16425
SFI1Q123692EBI-4259,EBI-2213082
SUS1Q6WNK76EBI-4259,EBI-1251050
THP1Q082313EBI-4259,EBI-32097

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi34647. 59 interactions.
DIPiDIP-2273N.
IntActiP06704. 31 interactions.
MINTiMINT-551680.

Structurei

Secondary structure

1
161
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni10 – 167Combined sources
Helixi19 – 3214Combined sources
Beta strandi36 – 405Combined sources
Helixi42 – 5110Combined sources
Helixi58 – 6811Combined sources
Beta strandi72 – 743Combined sources
Helixi78 – 9013Combined sources
Helixi94 – 10512Combined sources
Beta strandi110 – 1134Combined sources
Helixi115 – 12410Combined sources
Helixi131 – 1399Combined sources
Beta strandi143 – 1508Combined sources
Helixi151 – 16010Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DOQX-ray3.00A/B/C1-161[»]
2GV5X-ray3.00A/B/D/E1-161[»]
3FWBX-ray2.50A1-161[»]
3FWCX-ray2.70A/E/I/M1-161[»]
4MBEX-ray2.61A/D1-161[»]
ProteinModelPortaliP06704.
SMRiP06704. Positions 9-161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06704.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 5536EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini56 – 9136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini93 – 12836EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini129 – 16133EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the centrin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00670000097718.
HOGENOMiHOG000233018.
InParanoidiP06704.
KOiK16466.
OMAiQEFFAIM.
OrthoDBiEOG7PK99H.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKNRSSLQS GPLNSELLEE QKQEIYEAFS LFDMNNDGFL DYHELKVAMK
60 70 80 90 100
ALGFELPKRE ILDLIDEYDS EGRHLMKYDD FYIVMGEKIL KRDPLDEIKR
110 120 130 140 150
AFQLFDDDHT GKISIKNLRR VAKELGETLT DEELRAMIEE FDLDGDGEIN
160
ENEFIAICTD S
Length:161
Mass (Da):18,751
Last modified:February 1, 1995 - v2
Checksum:i25A546E76E1EF520
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771K → L in AAA66893 (PubMed:3526331).Curated
Sequence conflicti110 – 1101T → I in AAA66893 (PubMed:3526331).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14078 Genomic DNA. Translation: AAA66893.1.
X74500 Genomic DNA. Translation: CAA52609.1.
Z75165 Genomic DNA. Translation: CAA99479.1.
AY558434 Genomic DNA. Translation: AAS56760.1.
BK006948 Genomic DNA. Translation: DAA11024.1.
PIRiS47549.
RefSeqiNP_014900.3. NM_001183676.3.

Genome annotation databases

EnsemblFungiiYOR257W; YOR257W; YOR257W.
GeneIDi854431.
KEGGisce:YOR257W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14078 Genomic DNA. Translation: AAA66893.1.
X74500 Genomic DNA. Translation: CAA52609.1.
Z75165 Genomic DNA. Translation: CAA99479.1.
AY558434 Genomic DNA. Translation: AAS56760.1.
BK006948 Genomic DNA. Translation: DAA11024.1.
PIRiS47549.
RefSeqiNP_014900.3. NM_001183676.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DOQX-ray3.00A/B/C1-161[»]
2GV5X-ray3.00A/B/D/E1-161[»]
3FWBX-ray2.50A1-161[»]
3FWCX-ray2.70A/E/I/M1-161[»]
4MBEX-ray2.61A/D1-161[»]
ProteinModelPortaliP06704.
SMRiP06704. Positions 9-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34647. 59 interactions.
DIPiDIP-2273N.
IntActiP06704. 31 interactions.
MINTiMINT-551680.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiP06704.

Proteomic databases

MaxQBiP06704.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR257W; YOR257W; YOR257W.
GeneIDi854431.
KEGGisce:YOR257W.

Organism-specific databases

EuPathDBiFungiDB:YOR257W.
SGDiS000005783. CDC31.

Phylogenomic databases

GeneTreeiENSGT00670000097718.
HOGENOMiHOG000233018.
InParanoidiP06704.
KOiK16466.
OMAiQEFFAIM.
OrthoDBiEOG7PK99H.

Enzyme and pathway databases

BioCyciYEAST:G3O-33748-MONOMER.
ReactomeiR-SCE-5696394. DNA Damage Recognition in GG-NER.
R-SCE-5696395. Formation of Incision Complex in GG-NER.

Miscellaneous databases

EvolutionaryTraceiP06704.
NextBioi976658.
PROiP06704.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast gene required for spindle pole body duplication: homology of its product with Ca2+-binding proteins."
    Baum P., Furlong C., Byers B.
    Proc. Natl. Acad. Sci. U.S.A. 83:5512-5516(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole body."
    Spang A., Courtney I., Grein K., Matzner M., Schiebel E.
    J. Cell Biol. 128:863-877(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: ATCC 204508 / S288c.
  3. "Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1 and VPH1."
    Poirey R., Jauniaux J.-C.
    Yeast 13:483-487(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Direct interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body."
    Biggins S., Rose M.D.
    J. Cell Biol. 125:843-852(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, KAR1-DEPENDENT RECRUITMENT TO SPB HALF-BRIDGE.
  8. "Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae."
    Vallen E.A., Ho W., Winey M., Rose M.D.
    Genetics 137:407-422(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The yeast centrin, cdc31p, and the interacting protein kinase, Kic1p, are required for cell integrity."
    Sullivan D.S., Biggins S., Rose M.D.
    J. Cell Biol. 143:751-765(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KIC1.
  10. "The yeast nuclear pore complex: composition, architecture, and transport mechanism."
    Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
    J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
  11. "Fine structure analysis of the yeast centrin, Cdc31p, identifies residues specific for cell morphology and spindle pole body duplication."
    Ivanovska I., Rose M.D.
    Genetics 157:503-518(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CELL MORPHOLOGY, MUTAGENESIS, STRUCTURE PREDICTION.
  12. "Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p."
    Jaspersen S.L., Giddings T.H. Jr., Winey M.
    J. Cell Biol. 159:945-956(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MPS3 AT THE SPB.
  13. "Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication."
    Kilmartin J.V.
    J. Cell Biol. 162:1211-1221(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SFI1 AT THE SPB.
  14. "Composition of the spindle pole body of Saccharomyces cerevisiae and the proteins involved in its duplication."
    Helfant A.H.
    Curr. Genet. 40:291-310(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  15. "Peering through the pore: nuclear pore complex structure, assembly, and function."
    Suntharalingam M., Wente S.R.
    Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-130, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCDC31_YEAST
AccessioniPrimary (citable) accession number: P06704
Secondary accession number(s): D6W2V8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.