Reviewed, UniProtKB/Swiss-Prot P06702 (S10A9_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein S100-A9
Alternative name(s):
    S100 calcium-binding protein A9
    Calgranulin-B
    Migration inhibitory factor-related protein 14
      Short name=MRP-14
      Short name=P14
    Leukocyte L1 complex heavy chain
    Calprotectin L1H subunit

Gene names

Name:	S100A9
Synonyms:	CAGB, CFAG, MRP14

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	114 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Expressed by macrophages in acutely inflammated tissues and in chronic inflammations. Seem to be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells.
Subunit structure	Interacts with CEACAM3 in a calcium-dependent manner. Ref.12
Miscellaneous	Has been shown to bind calcium.
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	Calcium
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell-cell signaling Ref.1 Traceable author statement. Source: ProtInc inflammatory response Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular function	calcium ion binding Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct signal transducer activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARRB1	P49407	1	EBI-1055001,EBI-743313
ARRB2	P32121	1	EBI-1055001,EBI-714559
ILK	Q13418	1	EBI-1055001,EBI-747644
NUAK1	O60285	1	EBI-1055001,EBI-1046789
PAK7	Q9P286	1	EBI-1055001,EBI-1051061
TAGLN	Q01995	1	EBI-1055001,EBI-1054248
USF2	Q15853	1	EBI-1055001,EBI-1055994

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 114

114

Protein S100-A9

PRO_0000143997

Regions

Domain

12 – 47

36

EF-hand 1

Domain

54 – 89

36

EF-hand 2

Calcium binding

23 – 36

14

1; low affinity Potential

Calcium binding

67 – 78

12

2; high affinity Potential

Amino acid modifications

Modified residue

2

1

Blocked amino end (Thr)

Modified residue

113

1

Phosphothreonine Ref.8 Ref.13

Natural variations

Natural variant

20

1

H → R Ref.4

VAR_013008

Experimental info

Sequence conflict

6

1

S → H AA sequence Ref.11

Sequence conflict

25

1

K → F AA sequence Ref.11

Sequence conflict

28

1

H → L AA sequence Ref.11

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

114

Helix Strand Turn

Details...

Helix

7 – 23

17

Beta strand

25 – 28

4

Helix

34 – 44

11

Turn

46 – 51

6

Helix

52 – 54

3

Helix

56 – 66

11

Helix

76 – 79

4

Helix

81 – 85

5

Helix

87 – 91

5

Sequences

	Sequence		Length	Mass (Da)
	P06702-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: C3BE19729E14C078	FASTA	114	13,242
	10 20 30 40 50 60 MTCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK KENKNEKVIE 70 80 90 100 110 HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG PGHHHKPGLG EGTP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis." Odink K., Cerletti N., Bruggen J., Clerc R.G., Tarcsay L., Zwaldo G., Gerhards G., Schlegel R., Sorg C. Nature 330:80-82(1987) [PubMed: 3313057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation." Lagasse E., Clerc R.G. Mol. Cell. Biol. 8:2402-2410(1988) [PubMed: 3405210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases." Murao S., Collart F.R., Huberman E. J. Biol. Chem. 264:8356-8360(1989) [PubMed: 2656677] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], BLOCKED N-TERMINUS.
[4]	"Human gene for migration inhibitory factor-related protein 14 (MRP14), variant allele." Wang M., Xu X., Cai Y., Xu H., Han Y., Xu Z., Wu M. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-20.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[8]	"Ionomycin-regulated phosphorylation of the myeloid calcium-binding protein p14." Edgeworth J., Freemont P., Hogg N. Nature 342:189-192(1989) [PubMed: 2478889] [Abstract] Cited for: PROTEIN SEQUENCE OF 84-114, PHOSPHORYLATION AT THR-113.
[9]	"Amino acid sequences of 60B8 antigens induced in HL-60 cells by 1,25-dihydroxyvitamin D3. The antigens are identical with macrophage-related protein-14 and -8." Tobe T., Murakami K., Tomita M., Nozawa R. Chem. Pharm. Bull. 37:1576-1580(1989) [PubMed: 2776242] [Abstract] Cited for: PROTEIN SEQUENCE, BLOCKED N-TERMINUS.
[10]	"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes." Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J. Electrophoresis 13:960-969(1992) [PubMed: 1286667] [Abstract] Cited for: PROTEIN SEQUENCE OF 11-19; 26-37 AND 94-107. Tissue: Keratinocyte.
[11]	"In vitro antimicrobial activity of the human neutrophil cytosolic S-100 protein complex, calprotectin, against Capnocytophaga sputigena." Miyasaki K.T., Bodeau A.L., Murthy A.R., Lehrer R.I. J. Dent. Res. 72:517-523(1993) [PubMed: 8423249] [Abstract] Cited for: PROTEIN SEQUENCE OF 5-34.
[12]	"The microbial receptor CEACAM3 is linked to the calprotectin complex in granulocytes." Streichert T., Ebrahimnejad A., Ganzer S., Flayeh R., Wagener C., Bruemmer J. Biochem. Biophys. Res. Commun. 289:191-197(2001) [PubMed: 11708798] [Abstract] Cited for: INTERACTION WITH CEACAM3.
[13]	"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-113, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X06233 mRNA. Translation: CAA29579.1.
M21064 Genomic DNA. Translation: AAA36326.1.
M26311 mRNA. Translation: AAA68480.1.
AF237581 Genomic DNA. Translation: AAF62536.1.
AF237582 Genomic DNA. Translation: AAF62537.1.
CR542207 mRNA. Translation: CAG47003.1.
CR542224 mRNA. Translation: CAG47020.1.
AL591704 Genomic DNA. Translation: CAI19494.1.
BC047681 mRNA. Translation: AAH47681.1.

IPI

IPI00027462.

PIR

B31848.

RefSeq

NP_002956.1.

UniGene

Hs.112405

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IRJ	X-ray	2.10	A/B/C/D/E/F/G/H	2-114	[»]
1XK4	X-ray	1.80	C/D/G/H/K/L	4-114	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1166N.

IntAct

P06702. 15 interactions.

PTM databases

PhosphoSite

P06702.

2-D gel databases

SWISS-2DPAGE

P06702.

Aarhus/Ghent-2DPAGE

5007. IEF.
6010. IEF.
6017. IEF.
7013. IEF.

OGP

P06702.

PMMA-2DPAGE

P06702.

Proteomic databases

PeptideAtlas

P06702.

PRIDE

P06702.

Genome annotation databases

Ensembl

ENSG00000163220. Homo sapiens. [Contig view]

GeneID

6280.

KEGG

hsa:6280.

Organism-specific databases

GeneCards

GC01P151596.

H-InvDB

HIX0023536.

HGNC

HGNC:10499. S100A9.

HPA

CAB009441.
HPA004193.

MIM

123886. gene.

PharmGKB

PA34911.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P06702.

HOVERGEN

P06702.

OMA

P06702. VASHEEM.

Gene expression databases

ArrayExpress

P06702.

Bgee

P06702.

CleanEx

HS_S100A9.

GermOnline

ENSG00000163220. Homo sapiens.

Family and domain databases

InterPro

IPR011992. EF-Hand_type.
IPR018248. EF_hand.
IPR018247. EF_HAND_1.
IPR018249. EF_HAND_2.
IPR001751. S100_Ca_bd.
IPR013787. S100_Ca_bd_sub.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

Pfam

PF00036. efhand. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]

ProDom

PD003407. CaBP_S100. 1 hit.
PD000012. EF-hand. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

24377.

SOURCE

Search...

Entry information

Entry name

S10A9_HUMAN

Accession

Primary (citable) accession number: P06702
Secondary accession number(s): Q6FGA1, Q9NYM0, Q9UCJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)