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Reviewed, UniProtKB/Swiss-Prot P06702 (S10A9_HUMAN)

Last modified June 16, 2009. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Protein S100-A9
Alternative name(s):
    S100 calcium-binding protein A9
    Calgranulin-B
    Migration inhibitory factor-related protein 14
      Short name=MRP-14
      Short name=P14
    Leukocyte L1 complex heavy chain
    Calprotectin L1H subunit
Gene names
Name: S100A9
Synonyms: CAGB, CFAG, MRP14
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length114 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Expressed by macrophages in acutely inflammated tissues and in chronic inflammations. Seem to be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells.

Subunit structure

Interacts with CEACAM3 in a calcium-dependent manner. Ref.12

Miscellaneous

Has been shown to bind calcium.

Sequence similarities

Belongs to the S-100 family.

Contains 2 EF-hand domains.

Ontologies

Keywords
   Coding sequence diversityPolymorphism
   DomainRepeat
   LigandCalcium
   PTMPhosphoprotein
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological processcell-cell signaling Ref.1

Traceable author statement. Source: ProtInc

inflammatory response Ref.1

Traceable author statement. Source: ProtInc

   Cellular componentcytoplasm

Inferred from direct assay. Source: HPA

nucleus

Inferred from direct assay. Source: HPA

   Molecular functioncalcium ion binding

Traceable author statement. Source: ProtInc

protein binding

Inferred from physical interaction. Source: IntAct

signal transducer activity Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 114114Protein S100-A9
PRO_0000143997

Regions

Domain12 – 4736EF-hand 1
Domain54 – 8936EF-hand 2
Calcium binding23 – 36141; low affinity Potential
Calcium binding67 – 78122; high affinity Potential

Amino acid modifications

Modified residue21Blocked amino end (Thr)
Modified residue1131Phosphothreonine Ref.8 Ref.13

Natural variations

Natural variant201H → R Ref.4
VAR_013008

Experimental info

Sequence conflict61S → H AA sequence Ref.11
Sequence conflict251K → F AA sequence Ref.11
Sequence conflict281H → L AA sequence Ref.11

Secondary structure

.................. 114
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06702-1 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: C3BE19729E14C078

FASTA11413,242
        10         20         30         40         50         60 
MTCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK KENKNEKVIE 

        70         80         90        100        110 
HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG PGHHHKPGLG EGTP 

« Hide

References

« Hide 'large scale' references
[1]"Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis."
Odink K., Cerletti N., Bruggen J., Clerc R.G., Tarcsay L., Zwaldo G., Gerhards G., Schlegel R., Sorg C.
Nature 330:80-82(1987) [PubMed: 3313057] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation."
Lagasse E., Clerc R.G.
Mol. Cell. Biol. 8:2402-2410(1988) [PubMed: 3405210] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases."
Murao S., Collart F.R., Huberman E.
J. Biol. Chem. 264:8356-8360(1989) [PubMed: 2656677] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], BLOCKED N-TERMINUS.
[4]"Human gene for migration inhibitory factor-related protein 14 (MRP14), variant allele."
Wang M., Xu X., Cai Y., Xu H., Han Y., Xu Z., Wu M.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-20.
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[8]"Ionomycin-regulated phosphorylation of the myeloid calcium-binding protein p14."
Edgeworth J., Freemont P., Hogg N.
Nature 342:189-192(1989) [PubMed: 2478889] [Abstract]
Cited for: PROTEIN SEQUENCE OF 84-114, PHOSPHORYLATION AT THR-113.
[9]"Amino acid sequences of 60B8 antigens induced in HL-60 cells by 1,25-dihydroxyvitamin D3. The antigens are identical with macrophage-related protein-14 and -8."
Tobe T., Murakami K., Tomita M., Nozawa R.
Chem. Pharm. Bull. 37:1576-1580(1989) [PubMed: 2776242] [Abstract]
Cited for: PROTEIN SEQUENCE, BLOCKED N-TERMINUS.
[10]"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."
Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.
Electrophoresis 13:960-969(1992) [PubMed: 1286667] [Abstract]
Cited for: PROTEIN SEQUENCE OF 11-19; 26-37 AND 94-107.
Tissue: Keratinocyte.
[11]"In vitro antimicrobial activity of the human neutrophil cytosolic S-100 protein complex, calprotectin, against Capnocytophaga sputigena."
Miyasaki K.T., Bodeau A.L., Murthy A.R., Lehrer R.I.
J. Dent. Res. 72:517-523(1993) [PubMed: 8423249] [Abstract]
Cited for: PROTEIN SEQUENCE OF 5-34.
[12]"The microbial receptor CEACAM3 is linked to the calprotectin complex in granulocytes."
Streichert T., Ebrahimnejad A., Ganzer S., Flayeh R., Wagener C., Bruemmer J.
Biochem. Biophys. Res. Commun. 289:191-197(2001) [PubMed: 11708798] [Abstract]
Cited for: INTERACTION WITH CEACAM3.
[13]"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-113, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

X06233 mRNA. Translation: CAA29579.1.
M21064 Genomic DNA. Translation: AAA36326.1.
M26311 mRNA. Translation: AAA68480.1.
AF237581 Genomic DNA. Translation: AAF62536.1.
AF237582 Genomic DNA. Translation: AAF62537.1.
CR542207 mRNA. Translation: CAG47003.1.
CR542224 mRNA. Translation: CAG47020.1.
AL591704 Genomic DNA. Translation: CAI19494.1.
BC047681 mRNA. Translation: AAH47681.1.
IPIIPI00027462.
PIRB31848.
RefSeqNP_002956.1.
UniGeneHs.112405

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1IRJX-ray2.10A/B/C/D/E/F/G/H2-114[»]
1XK4X-ray1.80C/D/G/H/K/L4-114[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1166N.
IntActP06702. 15 interactions.

PTM databases

PhosphoSiteP06702.

2-D gel databases

SWISS-2DPAGEP06702.
Aarhus/Ghent-2DPAGE5007. IEF.
6010. IEF.
6017. IEF.
7013. IEF.
OGPP06702.
PMMA-2DPAGEP06702.

Proteomic databases

PeptideAtlasP06702.
PRIDEP06702.

Genome annotation databases

EnsemblENSG00000163220. Homo sapiens. [Contig view]
GeneID6280.
KEGGhsa:6280.

Organism-specific databases

GeneCardsGC01P151596.
H-InvDBHIX0023536.
HGNCHGNC:10499. S100A9.
HPACAB009441.
HPA004193.
MIM123886. gene.
PharmGKBPA34911.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP06702.
HOVERGENP06702.
OMAP06702. VASHEEM.

Gene expression databases

ArrayExpressP06702.
BgeeP06702.
CleanExHS_S100A9.
GermOnlineENSG00000163220. Homo sapiens.

Family and domain databases

InterProIPR011992. EF-Hand_type.
IPR018248. EF_hand.
IPR018247. EF_HAND_1.
IPR018249. EF_HAND_2.
IPR001751. S100_Ca_bd.
IPR013787. S100_Ca_bd_sub.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 1 hit.
PfamPF00036. efhand. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]
ProDomPD003407. CaBP_S100. 1 hit.
PD000012. EF-hand. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio24377.
SOURCESearch...

Entry information

Entry nameS10A9_HUMAN
AccessionPrimary (citable) accession number: P06702
Secondary accession number(s): Q6FGA1, Q9NYM0, Q9UCJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 16, 2009
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents