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P06687

- AT1A3_RAT

UniProt

P06687 - AT1A3_RAT

Protein

Sodium/potassium-transporting ATPase subunit alpha-3

Gene

Atp1a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 2 (18 Oct 2001)
      Previous versions | rss
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    Functioni

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

    Catalytic activityi

    ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei366 – 36614-aspartylphosphate intermediateBy similarity
    Metal bindingi707 – 7071MagnesiumBy similarity
    Metal bindingi711 – 7111MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: RGD
    2. cation-transporting ATPase activity Source: RGD
    3. D1 dopamine receptor binding Source: RGD
    4. metal ion binding Source: UniProtKB-KW
    5. protein binding Source: UniProtKB
    6. sodium:potassium-exchanging ATPase activity Source: UniProtKB

    GO - Biological processi

    1. adult locomotory behavior Source: Ensembl
    2. ATP biosynthetic process Source: InterPro
    3. ATP hydrolysis coupled proton transport Source: RGD
    4. ionotropic glutamate receptor signaling pathway Source: Ensembl
    5. memory Source: Ensembl
    6. potassium ion transport Source: RGD
    7. response to drug Source: Ensembl
    8. sodium ion transmembrane transport Source: GOC
    9. sodium ion transport Source: RGD
    10. visual learning Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

    Enzyme and pathway databases

    SABIO-RKP06687.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/potassium-transporting ATPase subunit alpha-3 (EC:3.6.3.9)
    Short name:
    Na(+)/K(+) ATPase alpha-3 subunit
    Alternative name(s):
    Na(+)/K(+) ATPase alpha(III) subunit
    Sodium pump subunit alpha-3
    Gene namesi
    Name:Atp1a3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi2169. Atp1a3.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. axon Source: RGD
    2. dendritic spine head Source: RGD
    3. dendritic spine neck Source: RGD
    4. endoplasmic reticulum Source: UniProtKB
    5. Golgi apparatus Source: UniProtKB
    6. myelin sheath Source: RGD
    7. nucleus Source: Ensembl
    8. plasma membrane Source: UniProtKB
    9. sodium:potassium-exchanging ATPase complex Source: RGD
    10. synapse Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10131013Sodium/potassium-transporting ATPase subunit alpha-3PRO_0000046300Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei265 – 2651PhosphoserineBy similarity
    Modified residuei548 – 5481PhosphotyrosineBy similarity
    Modified residuei933 – 9331Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP06687.
    PRIDEiP06687.

    PTM databases

    PhosphoSiteiP06687.

    Expressioni

    Gene expression databases

    GenevestigatoriP06687.

    Interactioni

    Subunit structurei

    Composed of three subunits: alpha (catalytic), beta and gamma.

    Protein-protein interaction databases

    BioGridi246401. 5 interactions.
    IntActiP06687. 2 interactions.
    MINTiMINT-7138201.

    Structurei

    3D structure databases

    ProteinModelPortaliP06687.
    SMRiP06687. Positions 20-1013.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7777CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini99 – 12123ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini143 – 278136CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini299 – 31012ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini329 – 762434CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini783 – 79210ExtracellularSequence Analysis
    Topological domaini814 – 83320CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini857 – 90852ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini929 – 94113CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini961 – 97515ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini997 – 101317CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei78 – 9821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei122 – 14221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei279 – 29820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei311 – 32818HelicalSequence AnalysisAdd
    BLAST
    Transmembranei763 – 78220HelicalSequence AnalysisAdd
    BLAST
    Transmembranei793 – 81321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei834 – 85623HelicalSequence AnalysisAdd
    BLAST
    Transmembranei909 – 92820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei942 – 96019HelicalSequence AnalysisAdd
    BLAST
    Transmembranei976 – 99621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni72 – 743Interaction with phosphoinositide-3 kinaseBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00560000076866.
    HOGENOMiHOG000265622.
    HOVERGENiHBG004298.
    KOiK01539.
    OMAiFQTHPEN.
    OrthoDBiEOG7327N0.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P06687-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDKKDDKSS PKKSKAKERR DLDDLKKEVA MTEHKMSVEE VCRKYNTDCV     50
    QGLTHSKAQE ILARDGPNAL TPPPTTPEWV KFCRQLFGGF SILLWIGAIL 100
    CFLAYGIQAG TEDDPSGDNL YLGIVLAAVV IITGCFSYYQ EAKSSKIMES 150
    FKNMVPQQAL VIREGEKMQV NAEEVVVGDL VEIKGGDRVP ADLRIISAHG 200
    CKVDNSSLTG ESEPQTRSPD CTHDNPLETR NITFFSTNCV EGTARGVVVA 250
    TGDRTVMGRI ATLASGLEVG KTPIAIEIEH FIQLITGVAV FLGVSFFILS 300
    LILGYTWLEA VIFLIGIIVA NVPEGLLATV TVCLTLTAKR MARKNCLVKN 350
    LEAVETLGST STICSDKTGT LTQNRMTVAH MWFDNQIHEA DTTEDQSGTS 400
    FDKSSHTWVA LSHIAGLCNR AVFKGGQDNI PVLKRDVAGD ASESALLKCI 450
    ELSSGSVKLM RERNKKVAEI PFNSTNKYQL SIHETEDPND NRYLLVMKGA 500
    PERILDRCAT ILLQGKEQPL DEEMKEAFQN AYLELGGLGE RVLGFCHYYL 550
    PEEQFPKGFA FDCDDVNFTT DNLCFVGLMS MIDPPRAAVP DAVGKCRSAG 600
    IKVIMVTGDH PITAKAIAKG VGIISEGNET VEDIAARLNI PVSQVNPRDA 650
    KACVIHGTDL KDFTSEQIDE ILQNHTEIVF ARTSPQQKLI IVEGCQRQGA 700
    IVAVTGDGVN DSPALKKADI GVAMGIAGSD VSKQAADMIL LDDNFASIVT 750
    GVEEGRLIFD NLKKSIAYTL TSNIPEITPF LLFIMANIPL PLGTITILCI 800
    DLGTDMVPAI SLAYEAAESD IMKRQPRNPR TDKLVNERLI SMAYGQIGMI 850
    QALGGFFSYF VILAENGFLP GNLVGIRLNW DDRTVNDLED SYGQQWTYEQ 900
    RKVVEFTCHT AFFVSIVVVQ WADLIICKTR RNSVFQQGMK NKILIFGLFE 950
    ETALAAFLSY CPGMDVALRM YPLKPSWWFC AFPYSFLIFV YDEIRKLILR 1000
    RNPGGWVEKE TYY 1013
    Length:1,013
    Mass (Da):111,692
    Last modified:October 18, 2001 - v2
    Checksum:i72F051406284EA8A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 33MGD → MNL in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti104 – 1041A → R in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti114 – 1141D → E in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti128 – 1281A → G in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti149 – 1491E → Q in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti152 – 1521K → T in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti166 – 1661E → D in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti192 – 1943DLR → ELG in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti200 – 2001G → R in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti339 – 3391Missing in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti621 – 6211V → K in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti807 – 8093VPA → DPT in AAA41672. (PubMed:2822726)Curated
    Sequence conflicti908 – 9081C → F in AAA40777. (PubMed:3028470)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14513 mRNA. Translation: AAA40777.1.
    X05883 mRNA. Translation: CAA29307.1.
    M28648 mRNA. Translation: AAA41672.1.
    PIRiC24639.
    RefSeqiNP_036638.1. NM_012506.1.
    UniGeneiRn.87329.

    Genome annotation databases

    EnsembliENSRNOT00000027497; ENSRNOP00000027497; ENSRNOG00000020263.
    GeneIDi24213.
    KEGGirno:24213.
    UCSCiRGD:2169. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14513 mRNA. Translation: AAA40777.1 .
    X05883 mRNA. Translation: CAA29307.1 .
    M28648 mRNA. Translation: AAA41672.1 .
    PIRi C24639.
    RefSeqi NP_036638.1. NM_012506.1.
    UniGenei Rn.87329.

    3D structure databases

    ProteinModelPortali P06687.
    SMRi P06687. Positions 20-1013.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246401. 5 interactions.
    IntActi P06687. 2 interactions.
    MINTi MINT-7138201.

    PTM databases

    PhosphoSitei P06687.

    Proteomic databases

    PaxDbi P06687.
    PRIDEi P06687.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027497 ; ENSRNOP00000027497 ; ENSRNOG00000020263 .
    GeneIDi 24213.
    KEGGi rno:24213.
    UCSCi RGD:2169. rat.

    Organism-specific databases

    CTDi 478.
    RGDi 2169. Atp1a3.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00560000076866.
    HOGENOMi HOG000265622.
    HOVERGENi HBG004298.
    KOi K01539.
    OMAi FQTHPEN.
    OrthoDBi EOG7327N0.

    Enzyme and pathway databases

    SABIO-RK P06687.

    Miscellaneous databases

    NextBioi 602625.

    Gene expression databases

    Genevestigatori P06687.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain."
      Shull G.E., Greeb J., Lingrel J.B.
      Biochemistry 25:8125-8132(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Primary structures of two types of alpha-subunit of rat brain Na+,K+,-ATPase deduced from cDNA sequences."
      Hara Y., Urayama O., Kawakami K., Nojima H., Nagamune H., Kojima T., Ohta T., Nagano K., Nakao M.
      J. Biochem. 102:43-58(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "Three differentially expressed Na,K-ATPase alpha subunit isoforms: structural and functional implications."
      Herrera V.L.M., Emanuel J.R., Ruiz-Opazo N., Levenson R., Nadal-Ginard B.
      J. Cell Biol. 105:1855-1865(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-930.
      Tissue: Brain and Liver.
    4. Lubec G., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 58-64; 147-152; 246-254; 260-271; 425-434; 436-448; 517-525; 587-595; 620-648; 734-763 AND 878-883, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiAT1A3_RAT
    AccessioniPrimary (citable) accession number: P06687
    Secondary accession number(s): Q16732, Q9Z1G6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: October 18, 2001
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3