Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sodium/potassium-transporting ATPase subunit alpha-2

Gene

Atp1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei374 – 37414-aspartylphosphate intermediateBy similarity
Metal bindingi714 – 7141MagnesiumBy similarity
Metal bindingi718 – 7181MagnesiumBy similarity

GO - Molecular functioni

  • ATP binding Source: RGD
  • cation-transporting ATPase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • sodium:potassium-exchanging ATPase activity Source: RGD
  • steroid hormone binding Source: BHF-UCL

GO - Biological processi

  • adult locomotory behavior Source: Ensembl
  • ATP hydrolysis coupled proton transport Source: RGD
  • cardiac muscle contraction Source: BHF-UCL
  • cellular potassium ion homeostasis Source: Ensembl
  • cellular response to mechanical stimulus Source: RGD
  • cellular response to steroid hormone stimulus Source: BHF-UCL
  • cellular sodium ion homeostasis Source: Ensembl
  • energy coupled proton transmembrane transport, against electrochemical gradient Source: RGD
  • locomotion Source: Ensembl
  • negative regulation of calcium:sodium antiporter activity Source: BHF-UCL
  • negative regulation of calcium ion transmembrane transport Source: BHF-UCL
  • negative regulation of cytosolic calcium ion concentration Source: Ensembl
  • negative regulation of heart contraction Source: Ensembl
  • negative regulation of striated muscle contraction Source: Ensembl
  • neurotransmitter uptake Source: Ensembl
  • potassium ion import Source: Ensembl
  • potassium ion transport Source: RGD
  • regulation of blood pressure Source: Ensembl
  • regulation of cardiac muscle cell contraction Source: RGD
  • regulation of cardiac muscle contraction by calcium ion signaling Source: BHF-UCL
  • regulation of respiratory gaseous exchange by neurological system process Source: Ensembl
  • regulation of smooth muscle contraction Source: Ensembl
  • regulation of the force of heart contraction Source: Ensembl
  • regulation of vasoconstriction Source: Ensembl
  • relaxation of cardiac muscle Source: BHF-UCL
  • response to glycoside Source: BHF-UCL
  • response to nicotine Source: RGD
  • sodium ion export from cell Source: Ensembl
  • sodium ion transport Source: RGD
  • visual learning Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

ReactomeiREACT_345620. Ion transport by P-type ATPases.
SABIO-RKP06686.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-2 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-2 subunit
Alternative name(s):
Na(+)/K(+) ATPase alpha(+) subunit
Sodium pump subunit alpha-2
Gene namesi
Name:Atp1a2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi2168. Atp1a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini6 – 8580CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei86 – 10621HelicalSequence AnalysisAdd
BLAST
Topological domaini107 – 12923ExtracellularSequence AnalysisAdd
BLAST
Transmembranei130 – 15021HelicalSequence AnalysisAdd
BLAST
Topological domaini151 – 286136CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei287 – 30620HelicalSequence AnalysisAdd
BLAST
Topological domaini307 – 31812ExtracellularSequence AnalysisAdd
BLAST
Transmembranei319 – 33618HelicalSequence AnalysisAdd
BLAST
Topological domaini337 – 769433CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei770 – 78920HelicalSequence AnalysisAdd
BLAST
Topological domaini790 – 79910ExtracellularSequence Analysis
Transmembranei800 – 82021HelicalSequence AnalysisAdd
BLAST
Topological domaini821 – 84020CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei841 – 86323HelicalSequence AnalysisAdd
BLAST
Topological domaini864 – 91552ExtracellularSequence AnalysisAdd
BLAST
Transmembranei916 – 93520HelicalSequence AnalysisAdd
BLAST
Topological domaini936 – 94813CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei949 – 96719HelicalSequence AnalysisAdd
BLAST
Topological domaini968 – 98215ExtracellularSequence AnalysisAdd
BLAST
Transmembranei983 – 100321HelicalSequence AnalysisAdd
BLAST
Topological domaini1004 – 102017CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • caveola Source: RGD
  • cytosol Source: GOC
  • dendritic spine Source: RGD
  • endosome Source: RGD
  • intercalated disc Source: BHF-UCL
  • myelin sheath Source: Ensembl
  • neuron projection Source: RGD
  • sarcolemma Source: RGD
  • sodium:potassium-exchanging ATPase complex Source: RGD
  • synapse Source: RGD
  • T-tubule Source: BHF-UCL
  • vesicle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 55PRO_0000002507
Chaini6 – 10201015Sodium/potassium-transporting ATPase subunit alpha-2PRO_0000002508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei570 – 5701PhosphothreonineBy similarity
Modified residuei587 – 5871PhosphoserineBy similarity
Modified residuei940 – 9401Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP06686.
PRIDEiP06686.

PTM databases

PhosphoSiteiP06686.

Expressioni

Gene expression databases

GenevisibleiP06686. RN.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.

Protein-protein interaction databases

BioGridi246400. 8 interactions.
IntActiP06686. 1 interaction.
STRINGi10116.ENSRNOP00000054947.

Structurei

3D structure databases

ProteinModelPortaliP06686.
SMRiP06686. Positions 28-1020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 823Interaction with phosphoinositide-3 kinaseBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00800000124052.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP06686.
KOiK01539.
OMAiFIIINIP.
OrthoDBiEOG7327N0.
PhylomeDBiP06686.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030333. ATP1A2.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF229. PTHR24093:SF229. 1 hit.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06686-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG
60 70 80 90 100
RKYQVDLSKG LTNQRAQDIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI
110 120 130 140 150
LLWIGALLCF LAYGILAAME DEPSNDNLYL GIVLAAVVIV TGCFSYYQEA
160 170 180 190 200
KSSKIMDSFK NMVPQQALVI REGEKMQINA EEVVVGDLVE VKGGDRVPAD
210 220 230 240 250
LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG
260 270 280 290 300
TARGIVIATG DRTVMGRIAT LASGLEVGQT PIAMEIEHFI QLITGVAVFL
310 320 330 340 350
GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA
360 370 380 390 400
RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT
410 420 430 440 450
TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS
460 470 480 490 500
ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDSPQSH
510 520 530 540 550
VLVMKGAPER ILDRCSTILV QGKEIPLDKE MQDAFQNAYM ELGGLGERVL
560 570 580 590 600
GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV
610 620 630 640 650
GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS
660 670 680 690 700
QVNPREAKAC VVHGSDLKDM TSEQLDEILR DHTEIVFART SPQQKLIIVE
710 720 730 740 750
GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGISGSDVSK QAADMILLDD
760 770 780 790 800
NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG
810 820 830 840 850
TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNSQTDK LVNERLISMA
860 870 880 890 900
YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR TTNDLEDSYG
910 920 930 940 950
QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI
960 970 980 990 1000
LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE
1010 1020
VRKLILRRYP GGWVEKETYY
Length:1,020
Mass (Da):112,217
Last modified:January 1, 1988 - v1
Checksum:i5436E795BD5B4CFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14512 mRNA. Translation: AAA40776.1.
BC085764 mRNA. Translation: AAH85764.1.
D90049 Genomic DNA. Translation: BAA14102.1.
PIRiB24639.
I54059.
RefSeqiNP_036637.1. NM_012505.2.
UniGeneiRn.1042.
Rn.214222.

Genome annotation databases

EnsembliENSRNOT00000058143; ENSRNOP00000054947; ENSRNOG00000007290.
GeneIDi24212.
KEGGirno:24212.
UCSCiRGD:2168. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14512 mRNA. Translation: AAA40776.1.
BC085764 mRNA. Translation: AAH85764.1.
D90049 Genomic DNA. Translation: BAA14102.1.
PIRiB24639.
I54059.
RefSeqiNP_036637.1. NM_012505.2.
UniGeneiRn.1042.
Rn.214222.

3D structure databases

ProteinModelPortaliP06686.
SMRiP06686. Positions 28-1020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246400. 8 interactions.
IntActiP06686. 1 interaction.
STRINGi10116.ENSRNOP00000054947.

PTM databases

PhosphoSiteiP06686.

Proteomic databases

PaxDbiP06686.
PRIDEiP06686.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000058143; ENSRNOP00000054947; ENSRNOG00000007290.
GeneIDi24212.
KEGGirno:24212.
UCSCiRGD:2168. rat.

Organism-specific databases

CTDi477.
RGDi2168. Atp1a2.

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00800000124052.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP06686.
KOiK01539.
OMAiFIIINIP.
OrthoDBiEOG7327N0.
PhylomeDBiP06686.
TreeFamiTF312838.

Enzyme and pathway databases

ReactomeiREACT_345620. Ion transport by P-type ATPases.
SABIO-RKP06686.

Miscellaneous databases

NextBioi602621.
PROiP06686.

Gene expression databases

GenevisibleiP06686. RN.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030333. ATP1A2.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF229. PTHR24093:SF229. 1 hit.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain."
    Shull G.E., Greeb J., Lingrel J.B.
    Biochemistry 25:8125-8132(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Regulation of Na+,K(+)-ATPases. I. Cloning and analysis of the 5'-flanking region of the rat NKAA2 gene encoding the alpha 2 subunit."
    Kawakami K., Yagawa Y., Nagano K.
    Gene 91:267-270(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
  4. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 594-602; 645-655; 659-668 AND 741-770, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiAT1A2_RAT
AccessioniPrimary (citable) accession number: P06686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: July 22, 2015
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.