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P06686

- AT1A2_RAT

UniProt

P06686 - AT1A2_RAT

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Protein
Sodium/potassium-transporting ATPase subunit alpha-2
Gene
Atp1a2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei374 – 37414-aspartylphosphate intermediate By similarity
Metal bindingi714 – 7141Magnesium By similarity
Metal bindingi718 – 7181Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. cation-transporting ATPase activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: RGD
  5. sodium:potassium-exchanging ATPase activity Source: RGD

GO - Biological processi

  1. ATP biosynthetic process Source: InterPro
  2. ATP hydrolysis coupled proton transport Source: RGD
  3. adult locomotory behavior Source: Ensembl
  4. cellular response to mechanical stimulus Source: RGD
  5. energy coupled proton transmembrane transport, against electrochemical gradient Source: RGD
  6. locomotion Source: Ensembl
  7. negative regulation of cytosolic calcium ion concentration Source: Ensembl
  8. negative regulation of heart contraction Source: Ensembl
  9. negative regulation of striated muscle contraction Source: Ensembl
  10. neurotransmitter uptake Source: Ensembl
  11. potassium ion transport Source: RGD
  12. regulation of blood pressure Source: Ensembl
  13. regulation of cardiac muscle cell contraction Source: RGD
  14. regulation of respiratory gaseous exchange by neurological system process Source: Ensembl
  15. regulation of smooth muscle contraction Source: Ensembl
  16. regulation of the force of heart contraction Source: Ensembl
  17. regulation of vasoconstriction Source: Ensembl
  18. response to nicotine Source: RGD
  19. sodium ion transmembrane transport Source: GOC
  20. sodium ion transport Source: RGD
  21. visual learning Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

SABIO-RKP06686.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-2 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-2 subunit
Alternative name(s):
Na(+)/K(+) ATPase alpha(+) subunit
Sodium pump subunit alpha-2
Gene namesi
Name:Atp1a2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 13

Organism-specific databases

RGDi2168. Atp1a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini6 – 8580Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei86 – 10621Helical; Reviewed prediction
Add
BLAST
Topological domaini107 – 12923Extracellular Reviewed prediction
Add
BLAST
Transmembranei130 – 15021Helical; Reviewed prediction
Add
BLAST
Topological domaini151 – 286136Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei287 – 30620Helical; Reviewed prediction
Add
BLAST
Topological domaini307 – 31812Extracellular Reviewed prediction
Add
BLAST
Transmembranei319 – 33618Helical; Reviewed prediction
Add
BLAST
Topological domaini337 – 769433Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei770 – 78920Helical; Reviewed prediction
Add
BLAST
Topological domaini790 – 79910Extracellular Reviewed prediction
Transmembranei800 – 82021Helical; Reviewed prediction
Add
BLAST
Topological domaini821 – 84020Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei841 – 86323Helical; Reviewed prediction
Add
BLAST
Topological domaini864 – 91552Extracellular Reviewed prediction
Add
BLAST
Transmembranei916 – 93520Helical; Reviewed prediction
Add
BLAST
Topological domaini936 – 94813Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei949 – 96719Helical; Reviewed prediction
Add
BLAST
Topological domaini968 – 98215Extracellular Reviewed prediction
Add
BLAST
Transmembranei983 – 100321Helical; Reviewed prediction
Add
BLAST
Topological domaini1004 – 102017Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. T-tubule Source: RGD
  2. caveola Source: RGD
  3. dendritic spine Source: RGD
  4. endosome Source: RGD
  5. neuron projection Source: RGD
  6. sarcolemma Source: RGD
  7. sodium:potassium-exchanging ATPase complex Source: RGD
  8. synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 55
PRO_0000002507
Chaini6 – 10201015Sodium/potassium-transporting ATPase subunit alpha-2
PRO_0000002508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei570 – 5701Phosphothreonine By similarity
Modified residuei587 – 5871Phosphoserine By similarity
Modified residuei940 – 9401Phosphoserine; by PKA By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP06686.
PRIDEiP06686.

PTM databases

PhosphoSiteiP06686.

Expressioni

Gene expression databases

GenevestigatoriP06686.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.

Protein-protein interaction databases

BioGridi246400. 7 interactions.
IntActiP06686. 1 interaction.
STRINGi10116.ENSRNOP00000054947.

Structurei

3D structure databases

ProteinModelPortaliP06686.
SMRiP06686. Positions 28-1020.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 823Interaction with phosphoinositide-3 kinase By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00560000076866.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
KOiK01539.
OMAiIINIPLP.
OrthoDBiEOG7327N0.
PhylomeDBiP06686.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06686-1 [UniParc]FASTAAdd to Basket

« Hide

MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG     50
RKYQVDLSKG LTNQRAQDIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI 100
LLWIGALLCF LAYGILAAME DEPSNDNLYL GIVLAAVVIV TGCFSYYQEA 150
KSSKIMDSFK NMVPQQALVI REGEKMQINA EEVVVGDLVE VKGGDRVPAD 200
LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG 250
TARGIVIATG DRTVMGRIAT LASGLEVGQT PIAMEIEHFI QLITGVAVFL 300
GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA 350
RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT 400
TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS 450
ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDSPQSH 500
VLVMKGAPER ILDRCSTILV QGKEIPLDKE MQDAFQNAYM ELGGLGERVL 550
GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV 600
GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS 650
QVNPREAKAC VVHGSDLKDM TSEQLDEILR DHTEIVFART SPQQKLIIVE 700
GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGISGSDVSK QAADMILLDD 750
NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG 800
TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNSQTDK LVNERLISMA 850
YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR TTNDLEDSYG 900
QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI 950
LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE 1000
VRKLILRRYP GGWVEKETYY 1020
Length:1,020
Mass (Da):112,217
Last modified:January 1, 1988 - v1
Checksum:i5436E795BD5B4CFA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14512 mRNA. Translation: AAA40776.1.
BC085764 mRNA. Translation: AAH85764.1.
D90049 Genomic DNA. Translation: BAA14102.1.
PIRiB24639.
I54059.
RefSeqiNP_036637.1. NM_012505.2.
UniGeneiRn.1042.
Rn.214222.

Genome annotation databases

EnsembliENSRNOT00000058143; ENSRNOP00000054947; ENSRNOG00000007290.
GeneIDi24212.
KEGGirno:24212.
UCSCiRGD:2168. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14512 mRNA. Translation: AAA40776.1 .
BC085764 mRNA. Translation: AAH85764.1 .
D90049 Genomic DNA. Translation: BAA14102.1 .
PIRi B24639.
I54059.
RefSeqi NP_036637.1. NM_012505.2.
UniGenei Rn.1042.
Rn.214222.

3D structure databases

ProteinModelPortali P06686.
SMRi P06686. Positions 28-1020.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246400. 7 interactions.
IntActi P06686. 1 interaction.
STRINGi 10116.ENSRNOP00000054947.

PTM databases

PhosphoSitei P06686.

Proteomic databases

PaxDbi P06686.
PRIDEi P06686.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000058143 ; ENSRNOP00000054947 ; ENSRNOG00000007290 .
GeneIDi 24212.
KEGGi rno:24212.
UCSCi RGD:2168. rat.

Organism-specific databases

CTDi 477.
RGDi 2168. Atp1a2.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00560000076866.
HOGENOMi HOG000265622.
HOVERGENi HBG004298.
KOi K01539.
OMAi IINIPLP.
OrthoDBi EOG7327N0.
PhylomeDBi P06686.
TreeFami TF312838.

Enzyme and pathway databases

SABIO-RK P06686.

Miscellaneous databases

NextBioi 602621.

Gene expression databases

Genevestigatori P06686.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain."
    Shull G.E., Greeb J., Lingrel J.B.
    Biochemistry 25:8125-8132(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Regulation of Na+,K(+)-ATPases. I. Cloning and analysis of the 5'-flanking region of the rat NKAA2 gene encoding the alpha 2 subunit."
    Kawakami K., Yagawa Y., Nagano K.
    Gene 91:267-270(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
  4. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 594-602; 645-655; 659-668 AND 741-770, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiAT1A2_RAT
AccessioniPrimary (citable) accession number: P06686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 11, 2014
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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