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P06686

- AT1A2_RAT

UniProt

P06686 - AT1A2_RAT

Protein

Sodium/potassium-transporting ATPase subunit alpha-2

Gene

Atp1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

    Catalytic activityi

    ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei374 – 37414-aspartylphosphate intermediateBy similarity
    Metal bindingi714 – 7141MagnesiumBy similarity
    Metal bindingi718 – 7181MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: RGD
    2. cation-transporting ATPase activity Source: RGD
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: RGD
    5. sodium:potassium-exchanging ATPase activity Source: RGD

    GO - Biological processi

    1. adult locomotory behavior Source: Ensembl
    2. ATP biosynthetic process Source: InterPro
    3. ATP hydrolysis coupled proton transport Source: RGD
    4. cellular response to mechanical stimulus Source: RGD
    5. energy coupled proton transmembrane transport, against electrochemical gradient Source: RGD
    6. locomotion Source: Ensembl
    7. negative regulation of cytosolic calcium ion concentration Source: Ensembl
    8. negative regulation of heart contraction Source: Ensembl
    9. negative regulation of striated muscle contraction Source: Ensembl
    10. neurotransmitter uptake Source: Ensembl
    11. potassium ion transport Source: RGD
    12. regulation of blood pressure Source: Ensembl
    13. regulation of cardiac muscle cell contraction Source: RGD
    14. regulation of respiratory gaseous exchange by neurological system process Source: Ensembl
    15. regulation of smooth muscle contraction Source: Ensembl
    16. regulation of the force of heart contraction Source: Ensembl
    17. regulation of vasoconstriction Source: Ensembl
    18. response to nicotine Source: RGD
    19. sodium ion transmembrane transport Source: GOC
    20. sodium ion transport Source: RGD
    21. visual learning Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

    Enzyme and pathway databases

    SABIO-RKP06686.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/potassium-transporting ATPase subunit alpha-2 (EC:3.6.3.9)
    Short name:
    Na(+)/K(+) ATPase alpha-2 subunit
    Alternative name(s):
    Na(+)/K(+) ATPase alpha(+) subunit
    Sodium pump subunit alpha-2
    Gene namesi
    Name:Atp1a2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi2168. Atp1a2.

    Subcellular locationi

    Membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. caveola Source: RGD
    2. dendritic spine Source: RGD
    3. endosome Source: RGD
    4. neuron projection Source: RGD
    5. sarcolemma Source: RGD
    6. sodium:potassium-exchanging ATPase complex Source: RGD
    7. synapse Source: RGD
    8. T-tubule Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 55PRO_0000002507
    Chaini6 – 10201015Sodium/potassium-transporting ATPase subunit alpha-2PRO_0000002508Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei570 – 5701PhosphothreonineBy similarity
    Modified residuei587 – 5871PhosphoserineBy similarity
    Modified residuei940 – 9401Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP06686.
    PRIDEiP06686.

    PTM databases

    PhosphoSiteiP06686.

    Expressioni

    Gene expression databases

    GenevestigatoriP06686.

    Interactioni

    Subunit structurei

    Composed of three subunits: alpha (catalytic), beta and gamma.

    Protein-protein interaction databases

    BioGridi246400. 7 interactions.
    IntActiP06686. 1 interaction.
    STRINGi10116.ENSRNOP00000054947.

    Structurei

    3D structure databases

    ProteinModelPortaliP06686.
    SMRiP06686. Positions 28-1020.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini6 – 8580CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini107 – 12923ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini151 – 286136CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini307 – 31812ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini337 – 769433CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini790 – 79910ExtracellularSequence Analysis
    Topological domaini821 – 84020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini864 – 91552ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini936 – 94813CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini968 – 98215ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1004 – 102017CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei86 – 10621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei130 – 15021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei287 – 30620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei319 – 33618HelicalSequence AnalysisAdd
    BLAST
    Transmembranei770 – 78920HelicalSequence AnalysisAdd
    BLAST
    Transmembranei800 – 82021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei841 – 86323HelicalSequence AnalysisAdd
    BLAST
    Transmembranei916 – 93520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei949 – 96719HelicalSequence AnalysisAdd
    BLAST
    Transmembranei983 – 100321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni80 – 823Interaction with phosphoinositide-3 kinaseBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00560000076866.
    HOGENOMiHOG000265622.
    HOVERGENiHBG004298.
    KOiK01539.
    OMAiIINIPLP.
    OrthoDBiEOG7327N0.
    PhylomeDBiP06686.
    TreeFamiTF312838.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06686-1 [UniParc]FASTAAdd to Basket

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    MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG     50
    RKYQVDLSKG LTNQRAQDIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI 100
    LLWIGALLCF LAYGILAAME DEPSNDNLYL GIVLAAVVIV TGCFSYYQEA 150
    KSSKIMDSFK NMVPQQALVI REGEKMQINA EEVVVGDLVE VKGGDRVPAD 200
    LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG 250
    TARGIVIATG DRTVMGRIAT LASGLEVGQT PIAMEIEHFI QLITGVAVFL 300
    GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA 350
    RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT 400
    TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS 450
    ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDSPQSH 500
    VLVMKGAPER ILDRCSTILV QGKEIPLDKE MQDAFQNAYM ELGGLGERVL 550
    GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV 600
    GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS 650
    QVNPREAKAC VVHGSDLKDM TSEQLDEILR DHTEIVFART SPQQKLIIVE 700
    GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGISGSDVSK QAADMILLDD 750
    NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG 800
    TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNSQTDK LVNERLISMA 850
    YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR TTNDLEDSYG 900
    QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI 950
    LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE 1000
    VRKLILRRYP GGWVEKETYY 1020
    Length:1,020
    Mass (Da):112,217
    Last modified:January 1, 1988 - v1
    Checksum:i5436E795BD5B4CFA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14512 mRNA. Translation: AAA40776.1.
    BC085764 mRNA. Translation: AAH85764.1.
    D90049 Genomic DNA. Translation: BAA14102.1.
    PIRiB24639.
    I54059.
    RefSeqiNP_036637.1. NM_012505.2.
    UniGeneiRn.1042.
    Rn.214222.

    Genome annotation databases

    EnsembliENSRNOT00000058143; ENSRNOP00000054947; ENSRNOG00000007290.
    GeneIDi24212.
    KEGGirno:24212.
    UCSCiRGD:2168. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14512 mRNA. Translation: AAA40776.1 .
    BC085764 mRNA. Translation: AAH85764.1 .
    D90049 Genomic DNA. Translation: BAA14102.1 .
    PIRi B24639.
    I54059.
    RefSeqi NP_036637.1. NM_012505.2.
    UniGenei Rn.1042.
    Rn.214222.

    3D structure databases

    ProteinModelPortali P06686.
    SMRi P06686. Positions 28-1020.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246400. 7 interactions.
    IntActi P06686. 1 interaction.
    STRINGi 10116.ENSRNOP00000054947.

    PTM databases

    PhosphoSitei P06686.

    Proteomic databases

    PaxDbi P06686.
    PRIDEi P06686.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000058143 ; ENSRNOP00000054947 ; ENSRNOG00000007290 .
    GeneIDi 24212.
    KEGGi rno:24212.
    UCSCi RGD:2168. rat.

    Organism-specific databases

    CTDi 477.
    RGDi 2168. Atp1a2.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00560000076866.
    HOGENOMi HOG000265622.
    HOVERGENi HBG004298.
    KOi K01539.
    OMAi IINIPLP.
    OrthoDBi EOG7327N0.
    PhylomeDBi P06686.
    TreeFami TF312838.

    Enzyme and pathway databases

    SABIO-RK P06686.

    Miscellaneous databases

    NextBioi 602621.

    Gene expression databases

    Genevestigatori P06686.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain."
      Shull G.E., Greeb J., Lingrel J.B.
      Biochemistry 25:8125-8132(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.
    3. "Regulation of Na+,K(+)-ATPases. I. Cloning and analysis of the 5'-flanking region of the rat NKAA2 gene encoding the alpha 2 subunit."
      Kawakami K., Yagawa Y., Nagano K.
      Gene 91:267-270(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
    4. Lubec G., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 594-602; 645-655; 659-668 AND 741-770, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiAT1A2_RAT
    AccessioniPrimary (citable) accession number: P06686
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 144 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3