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P06686

- AT1A2_RAT

UniProt

P06686 - AT1A2_RAT

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Protein

Sodium/potassium-transporting ATPase subunit alpha-2

Gene

Atp1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei374 – 37414-aspartylphosphate intermediateBy similarity
Metal bindingi714 – 7141MagnesiumBy similarity
Metal bindingi718 – 7181MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. cation-transporting ATPase activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW
  4. sodium:potassium-exchanging ATPase activity Source: RGD
  5. steroid hormone binding Source: BHF-UCL

GO - Biological processi

  1. adult locomotory behavior Source: Ensembl
  2. ATP biosynthetic process Source: InterPro
  3. ATP hydrolysis coupled proton transport Source: RGD
  4. cardiac muscle contraction Source: BHF-UCL
  5. cellular response to mechanical stimulus Source: RGD
  6. cellular response to steroid hormone stimulus Source: BHF-UCL
  7. energy coupled proton transmembrane transport, against electrochemical gradient Source: RGD
  8. locomotion Source: Ensembl
  9. negative regulation of calcium:sodium antiporter activity Source: BHF-UCL
  10. negative regulation of calcium ion transmembrane transport Source: BHF-UCL
  11. negative regulation of cytosolic calcium ion concentration Source: Ensembl
  12. negative regulation of heart contraction Source: Ensembl
  13. negative regulation of striated muscle contraction Source: Ensembl
  14. neurotransmitter uptake Source: Ensembl
  15. potassium ion transport Source: RGD
  16. regulation of blood pressure Source: Ensembl
  17. regulation of cardiac muscle cell contraction Source: RGD
  18. regulation of cardiac muscle contraction by calcium ion signaling Source: BHF-UCL
  19. regulation of respiratory gaseous exchange by neurological system process Source: Ensembl
  20. regulation of smooth muscle contraction Source: Ensembl
  21. regulation of the force of heart contraction Source: Ensembl
  22. regulation of vasoconstriction Source: Ensembl
  23. relaxation of cardiac muscle Source: BHF-UCL
  24. response to nicotine Source: RGD
  25. sodium ion transmembrane transport Source: GOC
  26. sodium ion transport Source: RGD
  27. visual learning Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

SABIO-RKP06686.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-2 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-2 subunit
Alternative name(s):
Na(+)/K(+) ATPase alpha(+) subunit
Sodium pump subunit alpha-2
Gene namesi
Name:Atp1a2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 13

Organism-specific databases

RGDi2168. Atp1a2.

Subcellular locationi

Membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity

GO - Cellular componenti

  1. caveola Source: RGD
  2. dendritic spine Source: RGD
  3. endosome Source: RGD
  4. intercalated disc Source: BHF-UCL
  5. neuron projection Source: RGD
  6. sarcolemma Source: RGD
  7. sodium:potassium-exchanging ATPase complex Source: RGD
  8. synapse Source: RGD
  9. T-tubule Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 55PRO_0000002507
Chaini6 – 10201015Sodium/potassium-transporting ATPase subunit alpha-2PRO_0000002508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei570 – 5701PhosphothreonineBy similarity
Modified residuei587 – 5871PhosphoserineBy similarity
Modified residuei940 – 9401Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP06686.
PRIDEiP06686.

PTM databases

PhosphoSiteiP06686.

Expressioni

Gene expression databases

GenevestigatoriP06686.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.

Protein-protein interaction databases

BioGridi246400. 8 interactions.
IntActiP06686. 1 interaction.
STRINGi10116.ENSRNOP00000054947.

Structurei

3D structure databases

ProteinModelPortaliP06686.
SMRiP06686. Positions 28-1020.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini6 – 8580CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini107 – 12923ExtracellularSequence AnalysisAdd
BLAST
Topological domaini151 – 286136CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini307 – 31812ExtracellularSequence AnalysisAdd
BLAST
Topological domaini337 – 769433CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini790 – 79910ExtracellularSequence Analysis
Topological domaini821 – 84020CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini864 – 91552ExtracellularSequence AnalysisAdd
BLAST
Topological domaini936 – 94813CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini968 – 98215ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1004 – 102017CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei86 – 10621HelicalSequence AnalysisAdd
BLAST
Transmembranei130 – 15021HelicalSequence AnalysisAdd
BLAST
Transmembranei287 – 30620HelicalSequence AnalysisAdd
BLAST
Transmembranei319 – 33618HelicalSequence AnalysisAdd
BLAST
Transmembranei770 – 78920HelicalSequence AnalysisAdd
BLAST
Transmembranei800 – 82021HelicalSequence AnalysisAdd
BLAST
Transmembranei841 – 86323HelicalSequence AnalysisAdd
BLAST
Transmembranei916 – 93520HelicalSequence AnalysisAdd
BLAST
Transmembranei949 – 96719HelicalSequence AnalysisAdd
BLAST
Transmembranei983 – 100321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 823Interaction with phosphoinositide-3 kinaseBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00760000119003.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP06686.
KOiK01539.
OMAiIINIPLP.
OrthoDBiEOG7327N0.
PhylomeDBiP06686.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06686-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG
60 70 80 90 100
RKYQVDLSKG LTNQRAQDIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI
110 120 130 140 150
LLWIGALLCF LAYGILAAME DEPSNDNLYL GIVLAAVVIV TGCFSYYQEA
160 170 180 190 200
KSSKIMDSFK NMVPQQALVI REGEKMQINA EEVVVGDLVE VKGGDRVPAD
210 220 230 240 250
LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG
260 270 280 290 300
TARGIVIATG DRTVMGRIAT LASGLEVGQT PIAMEIEHFI QLITGVAVFL
310 320 330 340 350
GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA
360 370 380 390 400
RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT
410 420 430 440 450
TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS
460 470 480 490 500
ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDSPQSH
510 520 530 540 550
VLVMKGAPER ILDRCSTILV QGKEIPLDKE MQDAFQNAYM ELGGLGERVL
560 570 580 590 600
GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV
610 620 630 640 650
GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS
660 670 680 690 700
QVNPREAKAC VVHGSDLKDM TSEQLDEILR DHTEIVFART SPQQKLIIVE
710 720 730 740 750
GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGISGSDVSK QAADMILLDD
760 770 780 790 800
NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG
810 820 830 840 850
TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNSQTDK LVNERLISMA
860 870 880 890 900
YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR TTNDLEDSYG
910 920 930 940 950
QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI
960 970 980 990 1000
LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE
1010 1020
VRKLILRRYP GGWVEKETYY
Length:1,020
Mass (Da):112,217
Last modified:January 1, 1988 - v1
Checksum:i5436E795BD5B4CFA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14512 mRNA. Translation: AAA40776.1.
BC085764 mRNA. Translation: AAH85764.1.
D90049 Genomic DNA. Translation: BAA14102.1.
PIRiB24639.
I54059.
RefSeqiNP_036637.1. NM_012505.2.
UniGeneiRn.1042.
Rn.214222.

Genome annotation databases

EnsembliENSRNOT00000058143; ENSRNOP00000054947; ENSRNOG00000007290.
GeneIDi24212.
KEGGirno:24212.
UCSCiRGD:2168. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14512 mRNA. Translation: AAA40776.1 .
BC085764 mRNA. Translation: AAH85764.1 .
D90049 Genomic DNA. Translation: BAA14102.1 .
PIRi B24639.
I54059.
RefSeqi NP_036637.1. NM_012505.2.
UniGenei Rn.1042.
Rn.214222.

3D structure databases

ProteinModelPortali P06686.
SMRi P06686. Positions 28-1020.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246400. 8 interactions.
IntActi P06686. 1 interaction.
STRINGi 10116.ENSRNOP00000054947.

PTM databases

PhosphoSitei P06686.

Proteomic databases

PaxDbi P06686.
PRIDEi P06686.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000058143 ; ENSRNOP00000054947 ; ENSRNOG00000007290 .
GeneIDi 24212.
KEGGi rno:24212.
UCSCi RGD:2168. rat.

Organism-specific databases

CTDi 477.
RGDi 2168. Atp1a2.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00760000119003.
HOGENOMi HOG000265622.
HOVERGENi HBG004298.
InParanoidi P06686.
KOi K01539.
OMAi IINIPLP.
OrthoDBi EOG7327N0.
PhylomeDBi P06686.
TreeFami TF312838.

Enzyme and pathway databases

SABIO-RK P06686.

Miscellaneous databases

NextBioi 602621.

Gene expression databases

Genevestigatori P06686.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain."
    Shull G.E., Greeb J., Lingrel J.B.
    Biochemistry 25:8125-8132(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Regulation of Na+,K(+)-ATPases. I. Cloning and analysis of the 5'-flanking region of the rat NKAA2 gene encoding the alpha 2 subunit."
    Kawakami K., Yagawa Y., Nagano K.
    Gene 91:267-270(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
  4. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 594-602; 645-655; 659-668 AND 741-770, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiAT1A2_RAT
AccessioniPrimary (citable) accession number: P06686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 29, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3