Reviewed, UniProtKB/Swiss-Prot P06683 (CO9_MOUSE)

Names and origin

Protein names

Recommended name:
    Complement component C9

Gene names

Name:

C9

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	C9 is the final component of the complement system to be added in the assembly of the membrane attack complex. It is able to enter lipid bilayers, forming transmembrane channels.
Subcellular location	Membrane; Multi-pass membrane protein Potential. Secreted.
Sequence similarities	Belongs to the complement C6/C7/C8/C9 family. Contains 1 EGF-like domain. Contains 1 LDL-receptor class A domain. Contains 1 MACPF domain. Contains 1 TSP type-1 domain.

Ontologies

Keywords
Biological process	Complement alternate pathway Complement pathway Cytolysis Immune response Innate immunity
Cellular component	Membrane Membrane attack complex Secreted
Domain	EGF-like domain Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	complement activation, alternative pathway Inferred from electronic annotation. Source: UniProtKB-KW complement activation, classical pathway Inferred from electronic annotation. Source: UniProtKB-KW cytolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane attack complex Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 548	528	Complement component C9		PRO_0000023605
Regions
	Transmembrane	312 – 328	17	Potential
	Transmembrane	333 – 352	20	Potential
	Domain	40 – 93	54	TSP type-1
	Domain	97 – 134	38	LDL-receptor class A
	Domain	136 – 512	377	MACPF
	Domain	513 – 543	31	EGF-like
Amino acid modifications
	Glycosylation	48	1	N-linked (GlcNAc...) Potential
	Glycosylation	263	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	417	1	N-linked (GlcNAc...) Potential
	Disulfide bond	41 ↔ 76		By similarity
	Disulfide bond	52 ↔ 55		By similarity
	Disulfide bond	86 ↔ 92		By similarity
	Disulfide bond	99 ↔ 110		By similarity
	Disulfide bond	105 ↔ 123		By similarity
	Disulfide bond	117 ↔ 132		By similarity
	Disulfide bond	140 ↔ 179		By similarity
	Disulfide bond	378 ↔ 407		By similarity
	Disulfide bond	513 ↔ 529		By similarity
	Disulfide bond	516 ↔ 531		By similarity
	Disulfide bond	533 ↔ 542		By similarity
Experimental info
	Sequence conflict	88	1	P → T in CAA29038. Ref.2
	Sequence conflict	153	1	T → R in CAA29038. Ref.2
	Sequence conflict	241	1	A → P in CAA29038. Ref.2
	Sequence conflict	246	1	E → Q in CAA29038. Ref.2
	Sequence conflict	261	1	P → A in CAA29038. Ref.2
	Sequence conflict	269 – 270	2	KF → TI in CAA29038. Ref.2
	Sequence conflict	285	1	F → L in CAA29038. Ref.2
	Sequence conflict	547	1	K → T in CAA29038. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P06683-1 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 8F1D16184E4781BE	FASTA	548	62,002
	10 20 30 40 50 60 MASGMAITLA LAIFALGVNA QMPIPVSREE QEQHYPIPID CRMSPWSNWS ECDPCLKQRF 70 80 90 100 110 120 RSRSILAFGQ FNGKSCVDVL GDRQGCEPTQ ECEEIQENCG NDFQCETGRC IKRRLLCNGD 130 140 150 160 170 180 NDCGDYSDEN DCDDDPRTPC RDRVAEESEL GLTAGYGINI LGMEPLRTPF DNEFYNGLCD 190 200 210 220 230 240 RVRDEKTYYR KPWNVVSLIY ETKADKSFRT ENYDEHLEVF KAINREKTSN FNADFALKFS 250 260 270 280 290 300 ATEVPEKGAG EVSPAEHSSK PTNISAKFKF SYFMGKNFRR LSSYFSQSKK MFVHLRGVVQ 310 320 330 340 350 360 LGRFVMRNRD VVLRSTFLDD VKALPTSYEK GEYFGFLETY GTHYSTSGSL GGQYEIVYVL 370 380 390 400 410 420 DKASMKEKGV DLNDVKHCLG FNMDLRIPLQ DDLKDASVTA SVNADGCIKT DNGKTVNITR 430 440 450 460 470 480 DNIIDDVISF IRGGTREQAI LLKEKILRGD KTFDKTDFAN WASSLANAPA LISQRMSPIY 490 500 510 520 530 540 NLIPLKIKDA YIKKQNLEKA VEDYIDEFST KRCYPCLNGG TIILLDGQCL CSCPMMFRGM ACEIHQKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[2]	"Topological mapping of complement component C9 by recombinant DNA techniques suggests a novel mechanism for its insertion into target membranes." Stanley K.K., Herz J. EMBO J. 6:1951-1957(1987) [PubMed: 2443347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-548.
[3]	"Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res. 5:2438-2447(2006) [PubMed: 16944957] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-263, MASS SPECTROMETRY. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC011137 mRNA. Translation: AAH11137.1. Different initiation. X05475 mRNA. Translation: CAA29038.1.
IPI	IPI00230718.
PIR	A29677.
RefSeq	NP_038513.1.
UniGene	Mm.29095
3D structure databases
HSSP	HSSP built from PDB template 1J8E based on UniProtKB Q07954.
ModBase	Search...
Proteomic databases
PRIDE	P06683.
Genome annotation databases
Ensembl	ENSMUSG00000022149. Mus musculus. [Contig view]
GeneID	12279.
KEGG	mmu:12279.
Organism-specific databases
MGI	MGI:1098282. C9.
Phylogenomic databases
HOGENOM	P06683.
HOVERGEN	P06683.
Gene expression databases
ArrayExpress	P06683.
Bgee	P06683.
CleanEx	MM_C9.
GermOnline	ENSMUSG00000022149. Mus musculus.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR002172. LDL_rcpt_classA_cys-rich. IPR001862. MAC_perforin. IPR000884. Thrombospondin_1_rpt. [Graphical view]
Gene3D	G3DSA:4.10.400.10. LDL_rcpt_classA_cys-rich. 1 hit.
Pfam	PF00057. Ldl_recept_a. 1 hit. PF01823. MACPF. 1 hit. PF00090. TSP_1. 1 hit. [Graphical view]
PRINTS	PR00764. COMPLEMENTC9.
SMART	SM00192. LDLa. 1 hit. SM00457. MACPF. 1 hit. SM00209. TSP1. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. False negative. PS50026. EGF_3. False negative. PS01209. LDLRA_1. 1 hit. PS50068. LDLRA_2. 1 hit. PS00279. MACPF_1. 1 hit. PS51412. MACPF_2. 1 hit. PS50092. TSP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	280740.
SOURCE	Search...

Entry information

Entry name

CO9_MOUSE

Accession

Primary (citable) accession number: P06683
Secondary accession number(s): Q91XA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2002
Last modified:	June 16, 2009
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)