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Protein

Complement component C9

Gene

C9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.By similarity

GO - Biological processi

Keywordsi

Biological processComplement alternate pathway, Complement pathway, Cytolysis, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Complement component C9
Gene namesi
Name:C9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1098282. C9.

Subcellular locationi

  • Secreted By similarity
  • Target cell membrane By similarity; Multi-pass membrane protein By similarity

  • Note: Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei312 – 328Beta strandedSequence analysisAdd BLAST17
Transmembranei333 – 352Beta strandedSequence analysisAdd BLAST20

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Membrane attack complex, Secreted, Target cell membrane, Target membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000002360521 – 548Complement component C9Add BLAST528

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 76By similarity
Glycosylationi48N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi52 ↔ 55By similarity
Disulfide bondi86 ↔ 92By similarity
Disulfide bondi99 ↔ 110By similarity
Disulfide bondi105 ↔ 123By similarity
Disulfide bondi117 ↔ 132By similarity
Disulfide bondi140 ↔ 179By similarity
Glycosylationi263N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi378 ↔ 407By similarity
Glycosylationi417N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi513 ↔ 529By similarity
Disulfide bondi516 ↔ 531By similarity
Disulfide bondi533 ↔ 542By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP06683.
PaxDbiP06683.
PeptideAtlasiP06683.
PRIDEiP06683.

PTM databases

iPTMnetiP06683.
PhosphoSitePlusiP06683.

Expressioni

Gene expression databases

CleanExiMM_C9.

Interactioni

Subunit structurei

Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9. About 20 C9 chains oligomerize to give rise to a huge beta-barrel that forms a 100 Angstrom diameter pore in target mebranes.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022749.

Structurei

3D structure databases

ProteinModelPortaliP06683.
SMRiP06683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 93TSP type-1PROSITE-ProRule annotationAdd BLAST54
Domaini97 – 134LDL-receptor class APROSITE-ProRule annotationAdd BLAST38
Domaini136 – 512MACPFPROSITE-ProRule annotationAdd BLAST377
Domaini513 – 543EGF-likeAdd BLAST31

Sequence similaritiesi

Belongs to the complement C6/C7/C8/C9 family.Curated

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG410IH3C. Eukaryota.
ENOG410XT9A. LUCA.
HOGENOMiHOG000111869.
HOVERGENiHBG106792.
InParanoidiP06683.
KOiK04000.
PhylomeDBiP06683.
TreeFamiTF330498.

Family and domain databases

InterProiView protein in InterPro
IPR009030. Growth_fac_rcpt_.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001862. MAC_perforin.
IPR020864. MACPF.
IPR020863. MACPF_CS.
IPR000884. TSP1_rpt.
PfamiView protein in Pfam
PF00057. Ldl_recept_a. 1 hit.
PF01823. MACPF. 1 hit.
PF00090. TSP_1. 1 hit.
PRINTSiPR00764. COMPLEMENTC9.
SMARTiView protein in SMART
SM00192. LDLa. 1 hit.
SM00457. MACPF. 1 hit.
SM00209. TSP1. 1 hit.
SUPFAMiSSF57184. SSF57184. 2 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiView protein in PROSITE
PS00022. EGF_1. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
PS50092. TSP1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGMAITLA LAIFALGVNA QMPIPVSREE QEQHYPIPID CRMSPWSNWS
60 70 80 90 100
ECDPCLKQRF RSRSILAFGQ FNGKSCVDVL GDRQGCEPTQ ECEEIQENCG
110 120 130 140 150
NDFQCETGRC IKRRLLCNGD NDCGDYSDEN DCDDDPRTPC RDRVAEESEL
160 170 180 190 200
GLTAGYGINI LGMEPLRTPF DNEFYNGLCD RVRDEKTYYR KPWNVVSLIY
210 220 230 240 250
ETKADKSFRT ENYDEHLEVF KAINREKTSN FNADFALKFS ATEVPEKGAG
260 270 280 290 300
EVSPAEHSSK PTNISAKFKF SYFMGKNFRR LSSYFSQSKK MFVHLRGVVQ
310 320 330 340 350
LGRFVMRNRD VVLRSTFLDD VKALPTSYEK GEYFGFLETY GTHYSTSGSL
360 370 380 390 400
GGQYEIVYVL DKASMKEKGV DLNDVKHCLG FNMDLRIPLQ DDLKDASVTA
410 420 430 440 450
SVNADGCIKT DNGKTVNITR DNIIDDVISF IRGGTREQAI LLKEKILRGD
460 470 480 490 500
KTFDKTDFAN WASSLANAPA LISQRMSPIY NLIPLKIKDA YIKKQNLEKA
510 520 530 540
VEDYIDEFST KRCYPCLNGG TIILLDGQCL CSCPMMFRGM ACEIHQKI
Length:548
Mass (Da):62,002
Last modified:January 23, 2002 - v2
Checksum:i8F1D16184E4781BE
GO

Sequence cautioni

The sequence AAH11137 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88P → T in CAA29038 (PubMed:2443347).Curated1
Sequence conflicti153T → R in CAA29038 (PubMed:2443347).Curated1
Sequence conflicti241A → P in CAA29038 (PubMed:2443347).Curated1
Sequence conflicti246E → Q in CAA29038 (PubMed:2443347).Curated1
Sequence conflicti261P → A in CAA29038 (PubMed:2443347).Curated1
Sequence conflicti269 – 270KF → TI in CAA29038 (PubMed:2443347).Curated2
Sequence conflicti285F → L in CAA29038 (PubMed:2443347).Curated1
Sequence conflicti547K → T in CAA29038 (PubMed:2443347).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC011137 mRNA. Translation: AAH11137.1. Different initiation.
X05475 mRNA. Translation: CAA29038.1.
PIRiA29677.
RefSeqiNP_038513.1. NM_013485.1.
UniGeneiMm.29095.

Genome annotation databases

GeneIDi12279.
KEGGimmu:12279.
UCSCiuc007vdh.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCO9_MOUSE
AccessioniPrimary (citable) accession number: P06683
Secondary accession number(s): Q91XA7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2002
Last modified: August 30, 2017
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families