P06668 (VIRD2_RHIRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-DNA border endonuclease virD2
EC=3.1.-.-

Gene names

Name:

virD2

Encoded on

Plasmid pTiA6NC

Organism

Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)

Taxonomic identifier

358 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function

Tumor formation by A.tumefaciens involves the transfer and integration of a defined segment (T-DNA) of Ti plasmid DNA into the plant nuclear genome. The virD operon encodes a site-specific endonuclease that cleaves at a unique site within both 24 bp direct repeats flanking the T-DNA.

Ontologies

Keywords
Disease	Crown gall tumor
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	induction by symbiont in host of tumor, nodule, or growth Inferred from electronic annotation. Source: InterPro nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC pathogenesis Inferred from electronic annotation. Source: InterPro
Molecular_function	endodeoxyribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	T-DNA border endonuclease virD2		PRO_0000065860

Sequences

	Sequence		Length	Mass (Da)
	P06668 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: B2CB7B82C01A6C1C	FASTA	424	47,546
	10 20 30 40 50 60 MPDRAQVIIR IVPGGGTKTL QQIINQLEYL SRKGKLELQR SARHLDIPVP PDQIRELAQS 70 80 90 100 110 120 WVTEAGIYDE SQSDDDRQQD LTTHIIVSFP AGTDQTAAYE ASREWAAEMF GSGYGGGRYN 130 140 150 160 170 180 YLTAYHVDRD HPHLHVVVNR RELLGHGWLK ISRRHPQLNY DGLRKKMAEI SLRHGIVLDA 190 200 210 220 230 240 TSRAERGIAE RPITYAEHRR LERMQAQKIQ FEDTDFDETS PEEDRRDLSQ SFDPFRSDPS 250 260 270 280 290 300 TGEPDRATRH DKQPLEQHAR FQESAGSSIK ADARIRVSLE SERSAQPSAS KIPVIGHFGI 310 320 330 340 350 360 ETSYVAEASV RKRSGIFGTS RPVTDVAMHT VKRQQRSKRR NDEEAGPSGA NRKGLKAAQV 370 380 390 400 410 420 DSEANVGEQD TRDDSNKAAD PVSASIGTEQ PEASPKRPRD RHDGELGGRK RARGNRRDDG RGGT

References

[1]	"The virD operon of Agrobacterium tumefaciens encodes a site-specific endonuclease." Yanofsky M.F., Porter S.G., Young C., Albright L.M., Gordon M.P., Nester E.W. Cell 47:471-477(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens." Jayaswal R.K., Veluthambi K., Gelvin S.B., Slightom J.L. J. Bacteriol. 169:5035-5045(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF242881 Genomic DNA. Translation: AAA98390.1. M17989 Genomic DNA. Translation: AAA22114.1.
PIR	B25063. B29826.
RefSeq	NP_059814.1. NC_002377.1.
3D structure databases
ProteinModelPortal	P06668.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29956N.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1224333.
Phylogenomic databases
ProtClustDB	PRK13863.
Family and domain databases
InterPro	IPR005094. Endonuclease_MobA/VirD2. IPR016644. Endonuclease_VirD2. [Graphical view]
Pfam	PF03432. Relaxase. 1 hit. [Graphical view]
PIRSF	PIRSF016095. Endonuclease_VirD2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VIRD2_RHIRD

Accession

Primary (citable) accession number: P06668

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program