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P06662

- NIFD_ACIFR

UniProt

P06662 - NIFD_ACIFR

Protein

Nitrogenase molybdenum-iron protein alpha chain

Gene

nifD

Organism
Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

    Catalytic activityi

    8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

    Cofactori

    Binds 1 8Fe-7S cluster per heterodimer.By similarity
    Binds 1 7Fe-Mo-9S-C-homocitryl cluster per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi71 – 711Iron-sulfur (8Fe-7S); shared with beta chainBy similarity
    Metal bindingi97 – 971Iron-sulfur (8Fe-7S); shared with beta chainBy similarity
    Metal bindingi163 – 1631Iron-sulfur (8Fe-7S); shared with beta chainBy similarity
    Metal bindingi284 – 2841Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl)By similarity
    Metal bindingi451 – 4511Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogenBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. carbonyl sulfide nitrogenase activity Source: UniProtKB-EC
    3. iron-sulfur cluster binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. nitrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Iron, Iron-sulfur, Metal-binding, Molybdenum, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrogenase molybdenum-iron protein alpha chain (EC:1.18.6.1)
    Alternative name(s):
    Dinitrogenase
    Nitrogenase component I
    Gene namesi
    Name:nifD
    OrganismiAcidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
    Taxonomic identifieri920 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus

    Subcellular locationi

    GO - Cellular componenti

    1. molybdenum-iron nitrogenase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 489489Nitrogenase molybdenum-iron protein alpha chainPRO_0000153084Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

    Structurei

    3D structure databases

    ProteinModelPortaliP06662.
    SMRiP06662. Positions 19-489.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NifD/NifK/NifE/NifN family.Curated

    Family and domain databases

    InterProiIPR000510. Nase/OxRdtase_comp1.
    IPR010143. Nase_comp1_asu.
    IPR000318. Nase_comp1_CS.
    IPR005972. Nase_Mo-Fe_asu.
    [Graphical view]
    PfamiPF00148. Oxidored_nitro. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01862. N2-ase-Ialpha. 1 hit.
    TIGR01282. nifD. 1 hit.
    PROSITEiPS00699. NITROGENASE_1_1. 1 hit.
    PS00090. NITROGENASE_1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P06662-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSISAEDLST QPQRRKLPEI AELIDETLKA YPEKFAKRRA KHLNVYEEGK    50
    SECDVKSNIK SVPGVMTIRG CAYAGSYGVV WSPVKDMIHI SHGPVGCGHY 100
    ARAGRRAYYI GTTGVDTYTT MHFTSDFQEK DIVFGGDKKL AKLMDELEEL 150
    FPMSKGITVQ SECPIGLIGD DIEAVSKKKA AEFGKPVVPN RCEGFRGVSQ 200
    SLGHHIANDS IRDWVLDPAA DKHPDFESTP YDVTLLGDYN IGGDWGSRII 250
    LEEMGLRVIA QWSGDAPSRS STASSKSKLN LLHCYRSVNY ITRHMEEKYG 300
    IPYIEFNFFG PTKIKESLRQ IAAFFDESIQ EKAEKAIAKY QPQWDAVVEK 350
    FRPRLEGKKV MLFVGGLRPG HTIGAFEDLG MEVIGTGYEF GHNDDYQRTT 400
    HEIKGNTLIY DDVTGYEFEK FAEKLRPDLV ASGVKEKYIF QKMGFPFRQM 450
    HSWDYSGPYH GPDGFAIFAR DMDMAVNNPV WGLTQAPWK 489
    Length:489
    Mass (Da):55,056
    Last modified:April 1, 1990 - v2
    Checksum:i5FE4F2166370EA4C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti55 – 551V → C(PubMed:3539923)Curated
    Sequence conflicti129 – 1291E → V(PubMed:3539923)Curated
    Sequence conflicti176 – 1761S → F(PubMed:3539923)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15238 Genomic DNA. Translation: AAA27375.1.
    PIRiA91597. NIBCAT.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15238 Genomic DNA. Translation: AAA27375.1 .
    PIRi A91597. NIBCAT.

    3D structure databases

    ProteinModelPortali P06662.
    SMRi P06662. Positions 19-489.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR000510. Nase/OxRdtase_comp1.
    IPR010143. Nase_comp1_asu.
    IPR000318. Nase_comp1_CS.
    IPR005972. Nase_Mo-Fe_asu.
    [Graphical view ]
    Pfami PF00148. Oxidored_nitro. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01862. N2-ase-Ialpha. 1 hit.
    TIGR01282. nifD. 1 hit.
    PROSITEi PS00699. NITROGENASE_1_1. 1 hit.
    PS00090. NITROGENASE_1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and structural analysis of the alpha- and beta-dinitrogenase subunits of Thiobacillus ferrooxidans."
      Rawlings D.E.
      Gene 69:337-343(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 33020 / 11Fe.
    2. "Nucleotide sequence of the gene encoding the nitrogenase iron protein of Thiobacillus ferrooxidans."
      Pretorius I.-M., Rawlings D.E., O'Neill E.G., Jones W.A., Kirby R., Woods D.R.
      J. Bacteriol. 169:367-370(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiNIFD_ACIFR
    AccessioniPrimary (citable) accession number: P06662
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3