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P06662

- NIFD_ACIFR

UniProt

P06662 - NIFD_ACIFR

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Protein
Nitrogenase molybdenum-iron protein alpha chain
Gene
nifD
Organism
Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

Binds 1 8Fe-7S cluster per heterodimer By similarity.
Binds 1 7Fe-Mo-9S-C-homocitryl cluster per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi71 – 711Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal bindingi97 – 971Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal bindingi163 – 1631Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal bindingi284 – 2841Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl) By similarity
Metal bindingi451 – 4511Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogen By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. carbonyl sulfide nitrogenase activity Source: UniProtKB-EC
  3. iron-sulfur cluster binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. nitrogenase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Iron, Iron-sulfur, Metal-binding, Molybdenum, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase molybdenum-iron protein alpha chain (EC:1.18.6.1)
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene namesi
Name:nifD
OrganismiAcidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Taxonomic identifieri920 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus

Subcellular locationi

GO - Cellular componenti

  1. molybdenum-iron nitrogenase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Nitrogenase molybdenum-iron protein alpha chain
PRO_0000153084Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Structurei

3D structure databases

ProteinModelPortaliP06662.
SMRiP06662. Positions 19-489.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR000510. Nase/OxRdtase_comp1.
IPR010143. Nase_comp1_asu.
IPR000318. Nase_comp1_CS.
IPR005972. Nase_Mo-Fe_asu.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01862. N2-ase-Ialpha. 1 hit.
TIGR01282. nifD. 1 hit.
PROSITEiPS00699. NITROGENASE_1_1. 1 hit.
PS00090. NITROGENASE_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06662-1 [UniParc]FASTAAdd to Basket

« Hide

MSISAEDLST QPQRRKLPEI AELIDETLKA YPEKFAKRRA KHLNVYEEGK    50
SECDVKSNIK SVPGVMTIRG CAYAGSYGVV WSPVKDMIHI SHGPVGCGHY 100
ARAGRRAYYI GTTGVDTYTT MHFTSDFQEK DIVFGGDKKL AKLMDELEEL 150
FPMSKGITVQ SECPIGLIGD DIEAVSKKKA AEFGKPVVPN RCEGFRGVSQ 200
SLGHHIANDS IRDWVLDPAA DKHPDFESTP YDVTLLGDYN IGGDWGSRII 250
LEEMGLRVIA QWSGDAPSRS STASSKSKLN LLHCYRSVNY ITRHMEEKYG 300
IPYIEFNFFG PTKIKESLRQ IAAFFDESIQ EKAEKAIAKY QPQWDAVVEK 350
FRPRLEGKKV MLFVGGLRPG HTIGAFEDLG MEVIGTGYEF GHNDDYQRTT 400
HEIKGNTLIY DDVTGYEFEK FAEKLRPDLV ASGVKEKYIF QKMGFPFRQM 450
HSWDYSGPYH GPDGFAIFAR DMDMAVNNPV WGLTQAPWK 489
Length:489
Mass (Da):55,056
Last modified:April 1, 1990 - v2
Checksum:i5FE4F2166370EA4C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551V → C1 Publication
Sequence conflicti129 – 1291E → V1 Publication
Sequence conflicti176 – 1761S → F1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15238 Genomic DNA. Translation: AAA27375.1.
PIRiA91597. NIBCAT.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15238 Genomic DNA. Translation: AAA27375.1 .
PIRi A91597. NIBCAT.

3D structure databases

ProteinModelPortali P06662.
SMRi P06662. Positions 19-489.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR000510. Nase/OxRdtase_comp1.
IPR010143. Nase_comp1_asu.
IPR000318. Nase_comp1_CS.
IPR005972. Nase_Mo-Fe_asu.
[Graphical view ]
Pfami PF00148. Oxidored_nitro. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01862. N2-ase-Ialpha. 1 hit.
TIGR01282. nifD. 1 hit.
PROSITEi PS00699. NITROGENASE_1_1. 1 hit.
PS00090. NITROGENASE_1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence and structural analysis of the alpha- and beta-dinitrogenase subunits of Thiobacillus ferrooxidans."
    Rawlings D.E.
    Gene 69:337-343(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 33020 / 11Fe.
  2. "Nucleotide sequence of the gene encoding the nitrogenase iron protein of Thiobacillus ferrooxidans."
    Pretorius I.-M., Rawlings D.E., O'Neill E.G., Jones W.A., Kirby R., Woods D.R.
    J. Bacteriol. 169:367-370(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiNIFD_ACIFR
AccessioniPrimary (citable) accession number: P06662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: April 1, 1990
Last modified: January 22, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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