Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrogenase molybdenum-iron protein alpha chain

Gene

nifD

Organism
Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi71Iron-sulfur (8Fe-7S); shared with beta chainBy similarity1
Metal bindingi97Iron-sulfur (8Fe-7S); shared with beta chainBy similarity1
Metal bindingi163Iron-sulfur (8Fe-7S); shared with beta chainBy similarity1
Metal bindingi284Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl)By similarity1
Metal bindingi451Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrogen fixation
LigandATP-binding, Iron, Iron-sulfur, Metal-binding, Molybdenum, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase molybdenum-iron protein alpha chain (EC:1.18.6.1)
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene namesi
Name:nifD
OrganismiAcidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Taxonomic identifieri920 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAcidithiobacilliaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001530841 – 489Nitrogenase molybdenum-iron protein alpha chainAdd BLAST489

Proteomic databases

PRIDEiP06662

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Structurei

3D structure databases

ProteinModelPortaliP06662
SMRiP06662
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifD/NifK/NifE/NifN family.Curated

Family and domain databases

CDDicd01976 Nitrogenase_MoFe_alpha, 1 hit
InterProiView protein in InterPro
IPR000510 Nase/OxRdtase_comp1
IPR010143 Nase_comp1_asu
IPR000318 Nase_comp1_CS
IPR005972 Nase_Mo-Fe_asu
PANTHERiPTHR43457 PTHR43457, 1 hit
PfamiView protein in Pfam
PF00148 Oxidored_nitro, 1 hit
TIGRFAMsiTIGR01862 N2-ase-Ialpha, 1 hit
TIGR01282 nifD, 1 hit
PROSITEiView protein in PROSITE
PS00699 NITROGENASE_1_1, 1 hit
PS00090 NITROGENASE_1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P06662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISAEDLST QPQRRKLPEI AELIDETLKA YPEKFAKRRA KHLNVYEEGK
60 70 80 90 100
SECDVKSNIK SVPGVMTIRG CAYAGSYGVV WSPVKDMIHI SHGPVGCGHY
110 120 130 140 150
ARAGRRAYYI GTTGVDTYTT MHFTSDFQEK DIVFGGDKKL AKLMDELEEL
160 170 180 190 200
FPMSKGITVQ SECPIGLIGD DIEAVSKKKA AEFGKPVVPN RCEGFRGVSQ
210 220 230 240 250
SLGHHIANDS IRDWVLDPAA DKHPDFESTP YDVTLLGDYN IGGDWGSRII
260 270 280 290 300
LEEMGLRVIA QWSGDAPSRS STASSKSKLN LLHCYRSVNY ITRHMEEKYG
310 320 330 340 350
IPYIEFNFFG PTKIKESLRQ IAAFFDESIQ EKAEKAIAKY QPQWDAVVEK
360 370 380 390 400
FRPRLEGKKV MLFVGGLRPG HTIGAFEDLG MEVIGTGYEF GHNDDYQRTT
410 420 430 440 450
HEIKGNTLIY DDVTGYEFEK FAEKLRPDLV ASGVKEKYIF QKMGFPFRQM
460 470 480
HSWDYSGPYH GPDGFAIFAR DMDMAVNNPV WGLTQAPWK
Length:489
Mass (Da):55,056
Last modified:April 1, 1990 - v2
Checksum:i5FE4F2166370EA4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55V → C (PubMed:3539923).Curated1
Sequence conflicti129E → V (PubMed:3539923).Curated1
Sequence conflicti176S → F (PubMed:3539923).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15238 Genomic DNA Translation: AAA27375.1
PIRiA91597 NIBCAT

Similar proteinsi

Entry informationi

Entry nameiNIFD_ACIFR
AccessioniPrimary (citable) accession number: P06662
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: April 1, 1990
Last modified: May 23, 2018
This is version 83 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health