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P06662 (NIFD_ACIFR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrogenase molybdenum-iron protein alpha chain

EC=1.18.6.1
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene names
Name:nifD
OrganismAcidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Taxonomic identifier920 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activity

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactor

Binds 1 8Fe-7S cluster per heterodimer By similarity.

Binds 1 7Fe-Mo-9S-C-homocitryl cluster per subunit By similarity.

Subunit structure

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Sequence similarities

Belongs to the NifD/NifK/NifE/NifN family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489Nitrogenase molybdenum-iron protein alpha chain
PRO_0000153084

Sites

Metal binding711Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding971Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding1631Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding2841Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl) By similarity
Metal binding4511Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogen By similarity

Experimental info

Sequence conflict551V → C Ref.2
Sequence conflict1291E → V Ref.2
Sequence conflict1761S → F Ref.2

Sequences

Sequence LengthMass (Da)Tools
P06662 [UniParc].

Last modified April 1, 1990. Version 2.
Checksum: 5FE4F2166370EA4C

FASTA48955,056
        10         20         30         40         50         60 
MSISAEDLST QPQRRKLPEI AELIDETLKA YPEKFAKRRA KHLNVYEEGK SECDVKSNIK 

        70         80         90        100        110        120 
SVPGVMTIRG CAYAGSYGVV WSPVKDMIHI SHGPVGCGHY ARAGRRAYYI GTTGVDTYTT 

       130        140        150        160        170        180 
MHFTSDFQEK DIVFGGDKKL AKLMDELEEL FPMSKGITVQ SECPIGLIGD DIEAVSKKKA 

       190        200        210        220        230        240 
AEFGKPVVPN RCEGFRGVSQ SLGHHIANDS IRDWVLDPAA DKHPDFESTP YDVTLLGDYN 

       250        260        270        280        290        300 
IGGDWGSRII LEEMGLRVIA QWSGDAPSRS STASSKSKLN LLHCYRSVNY ITRHMEEKYG 

       310        320        330        340        350        360 
IPYIEFNFFG PTKIKESLRQ IAAFFDESIQ EKAEKAIAKY QPQWDAVVEK FRPRLEGKKV 

       370        380        390        400        410        420 
MLFVGGLRPG HTIGAFEDLG MEVIGTGYEF GHNDDYQRTT HEIKGNTLIY DDVTGYEFEK 

       430        440        450        460        470        480 
FAEKLRPDLV ASGVKEKYIF QKMGFPFRQM HSWDYSGPYH GPDGFAIFAR DMDMAVNNPV 


WGLTQAPWK 

« Hide

References

[1]"Sequence and structural analysis of the alpha- and beta-dinitrogenase subunits of Thiobacillus ferrooxidans."
Rawlings D.E.
Gene 69:337-343(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 33020 / 11Fe.
[2]"Nucleotide sequence of the gene encoding the nitrogenase iron protein of Thiobacillus ferrooxidans."
Pretorius I.-M., Rawlings D.E., O'Neill E.G., Jones W.A., Kirby R., Woods D.R.
J. Bacteriol. 169:367-370(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M15238 Genomic DNA. Translation: AAA27375.1.
PIRNIBCAT. A91597.

3D structure databases

ProteinModelPortalP06662.
SMRP06662. Positions 19-489.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000510. Nase/OxRdtase_comp1.
IPR010143. Nase_comp1_asu.
IPR000318. Nase_comp1_CS.
IPR005972. Nase_Mo-Fe_asu.
[Graphical view]
PfamPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
TIGRFAMsTIGR01862. N2-ase-Ialpha. 1 hit.
TIGR01282. nifD. 1 hit.
PROSITEPS00699. NITROGENASE_1_1. 1 hit.
PS00090. NITROGENASE_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNIFD_ACIFR
AccessionPrimary (citable) accession number: P06662
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: April 1, 1990
Last modified: January 22, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families