P06652 (MPIP_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
M-phase inducer phosphatase
EC=3.1.3.48

Alternative name(s):
Mitosis initiation protein
P80

Gene names

Name:	cdc25
ORF Names:	SPAC24H6.05

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate p34(cdc2) and activate the p34(cdc2) kinase activity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Sequence similarities	Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.
Sequence caution	The sequence AAA35294.1 differs from that shown. Reason: Frameshift at position 571.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	M phase of mitotic cell cycle Inferred from electronic annotation. Source: InterPro cell division Inferred from electronic annotation. Source: UniProtKB-KW intracellular signal transduction Non-traceable author statement. Source: PomBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic DNA replication checkpoint Inferred from mutant phenotype PubMed 1549179. Source: PomBase peptidyl-tyrosine dephosphorylation involved in activation of protein kinase activity Inferred from direct assay PubMed 1756737. Source: PomBase positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M Traceable author statement PubMed 2665944. Source: PomBase regulation of G2/M transition of mitotic cell cycle Inferred from mutant phenotype Ref.1. Source: PomBase regulation of cell size Non-traceable author statement. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase nucleus Inferred from direct assay PubMed 1500423. Source: PomBase
Molecular_function	protein serine/threonine phosphatase activity Inferred from direct assay PubMed 1756737. Source: PomBase protein tyrosine phosphatase activity Inferred from direct assay PubMed 1819507. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	M-phase inducer phosphatase		PRO_0000198661
Regions
	Domain	429 – 533	105	Rhodanese
Sites
	Active site	480	1	By similarity
Amino acid modifications
	Modified residue	99	1	Phosphoserine Ref.3
	Modified residue	178	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P06652 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: CF8D430FC7BD38C1	FASTA	596	66,566
	10 20 30 40 50 60 MDSPLSSLSF TNTLSGKRNV LRPAARELKL MSDRNANQEL DFFFPKSKHI ASTLVDPFGK 70 80 90 100 110 120 TCSTASPASS LAADMSMNMH IDESPALPTP RRTLFRSLSC TVETPLANKT IVSPLPESPS 130 140 150 160 170 180 NDALTESYFF RQPASKYSIT QDSPRVSSTI AYSFKPKASI ALNTTKSEAT RSSLSSSSFD 190 200 210 220 230 240 SYLRPNVSRS RSSGNAPPFL RSRSSSSYSI NKKKGTSGGQ ATRHLTYALS RTCSQSSNTT 250 260 270 280 290 300 SLLESCLTDD TDDFELMSDH EDTFTMGKVA DLPESSVELV EDAASIQRPN SDFGACNDNS 310 320 330 340 350 360 LDDLFQASPI KPIDMLPKIN KDIAFPSLKV RSPSPMAFAM QEDAEYDEQD TPVLRRTQSM 370 380 390 400 410 420 FLNSTRLGLF KSQDLVCVTP KQSTKESERF ISSHVEDLSL PCFAVKEDSL KRITQETLLG 430 440 450 460 470 480 LLDGKFKDIF DKCIIIDCRF EYEYLGGHIS TAVNLNTKQA IVDAFLSKPL THRVALVFHC 490 500 510 520 530 540 EHSAHRAPHL ALHFRNTDRR MNSHRYPFLY YPEVYILHGG YKSFYENHKN RCDPINYVPM 550 560 570 580 590 NDASHVMTCT KAMNNFKRNA TFMRTKSYTF GQSVLASPDV NDSPTAMHSL STLRRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cdc25+ functions as an inducer in the mitotic control of fission yeast." Russell P., Nurse P. Cell 45:145-153(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99 AND SER-178, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M13158 Genomic DNA. Translation: AAA35294.1. Frameshift. CU329670 Genomic DNA. Translation: CAA90849.1.
PIR	S62407.
RefSeq	NP_592947.1. NM_001018348.2.
3D structure databases
ProteinModelPortal	P06652.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06652. 2 interactions.
MINT	MINT-4686889.
STRING	4896.SPAC24H6.05-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC24H6.05.1; SPAC24H6.05.1:pep; SPAC24H6.05.
GeneID	2542632.
KEGG	spo:SPAC24H6.05.
Organism-specific databases
PomBase	SPAC24H6.05.
Phylogenomic databases
eggNOG	COG5105.
KO	K01104.
OrthoDB	EOG4VQDZR.
Gene expression databases
GermOnline	SPAC24H6.05. Schizosaccharomyces pombe.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000751. MPI_Phosphatase. IPR001763. Rhodanese-like_dom. [Graphical view]
PANTHER	PTHR10828. PTHR10828. 1 hit.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR00716. MPIPHPHTASE.
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20803681.

Entry information

Entry name

MPIP_SCHPO

Accession

Primary (citable) accession number: P06652

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program