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P06652

- MPIP_SCHPO

UniProt

P06652 - MPIP_SCHPO

Protein

M-phase inducer phosphatase

Gene

cdc25

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate p34(cdc2) and activate the p34(cdc2) kinase activity.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei480 – 4801By similarity

    GO - Molecular functioni

    1. protein binding Source: PomBase
    2. protein serine/threonine phosphatase activity Source: PomBase
    3. protein tyrosine phosphatase activity Source: PomBase

    GO - Biological processi

    1. intracellular signal transduction Source: PomBase
    2. mitotic DNA replication checkpoint Source: PomBase
    3. mitotic nuclear division Source: UniProtKB-KW
    4. peptidyl-tyrosine dephosphorylation involved in activation of protein kinase activity Source: PomBase
    5. positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle Source: PomBase
    6. regulation of cell size Source: PomBase
    7. regulation of G2/M transition of mitotic cell cycle Source: PomBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_199398. Cyclin A/B1 associated events during G2/M transition.
    REACT_204375. Cyclin A:Cdk2-associated events at S phase entry.
    REACT_210479. p53-Independent DNA Damage Response.
    REACT_217024. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_218991. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_219991. Cyclin B2 mediated events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    M-phase inducer phosphatase (EC:3.1.3.48)
    Alternative name(s):
    Mitosis initiation protein
    P80
    Gene namesi
    Name:cdc25
    ORF Names:SPAC24H6.05
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC24H6.05.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. nucleus Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 596596M-phase inducer phosphatasePRO_0000198661Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei99 – 991Phosphoserine1 Publication
    Modified residuei178 – 1781Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP06652.

    Interactioni

    Protein-protein interaction databases

    BioGridi279086. 76 interactions.
    IntActiP06652. 2 interactions.
    MINTiMINT-4686889.
    STRINGi4896.SPAC24H6.05-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP06652.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini429 – 533105RhodanesePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5105.
    KOiK02555.
    OMAiISSHVED.
    OrthoDBiEOG7VMPGR.
    PhylomeDBiP06652.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR00716. MPIPHPHTASE.
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P06652-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSPLSSLSF TNTLSGKRNV LRPAARELKL MSDRNANQEL DFFFPKSKHI    50
    ASTLVDPFGK TCSTASPASS LAADMSMNMH IDESPALPTP RRTLFRSLSC 100
    TVETPLANKT IVSPLPESPS NDALTESYFF RQPASKYSIT QDSPRVSSTI 150
    AYSFKPKASI ALNTTKSEAT RSSLSSSSFD SYLRPNVSRS RSSGNAPPFL 200
    RSRSSSSYSI NKKKGTSGGQ ATRHLTYALS RTCSQSSNTT SLLESCLTDD 250
    TDDFELMSDH EDTFTMGKVA DLPESSVELV EDAASIQRPN SDFGACNDNS 300
    LDDLFQASPI KPIDMLPKIN KDIAFPSLKV RSPSPMAFAM QEDAEYDEQD 350
    TPVLRRTQSM FLNSTRLGLF KSQDLVCVTP KQSTKESERF ISSHVEDLSL 400
    PCFAVKEDSL KRITQETLLG LLDGKFKDIF DKCIIIDCRF EYEYLGGHIS 450
    TAVNLNTKQA IVDAFLSKPL THRVALVFHC EHSAHRAPHL ALHFRNTDRR 500
    MNSHRYPFLY YPEVYILHGG YKSFYENHKN RCDPINYVPM NDASHVMTCT 550
    KAMNNFKRNA TFMRTKSYTF GQSVLASPDV NDSPTAMHSL STLRRF 596
    Length:596
    Mass (Da):66,566
    Last modified:November 1, 1995 - v2
    Checksum:iCF8D430FC7BD38C1
    GO

    Sequence cautioni

    The sequence AAA35294.1 differs from that shown. Reason: Frameshift at position 571.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13158 Genomic DNA. Translation: AAA35294.1. Frameshift.
    CU329670 Genomic DNA. Translation: CAA90849.1.
    PIRiS62407.
    RefSeqiNP_592947.1. NM_001018348.2.

    Genome annotation databases

    EnsemblFungiiSPAC24H6.05.1; SPAC24H6.05.1:pep; SPAC24H6.05.
    GeneIDi2542632.
    KEGGispo:SPAC24H6.05.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13158 Genomic DNA. Translation: AAA35294.1 . Frameshift.
    CU329670 Genomic DNA. Translation: CAA90849.1 .
    PIRi S62407.
    RefSeqi NP_592947.1. NM_001018348.2.

    3D structure databases

    ProteinModelPortali P06652.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279086. 76 interactions.
    IntActi P06652. 2 interactions.
    MINTi MINT-4686889.
    STRINGi 4896.SPAC24H6.05-1.

    Proteomic databases

    MaxQBi P06652.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC24H6.05.1 ; SPAC24H6.05.1:pep ; SPAC24H6.05 .
    GeneIDi 2542632.
    KEGGi spo:SPAC24H6.05.

    Organism-specific databases

    PomBasei SPAC24H6.05.

    Phylogenomic databases

    eggNOGi COG5105.
    KOi K02555.
    OMAi ISSHVED.
    OrthoDBi EOG7VMPGR.
    PhylomeDBi P06652.

    Enzyme and pathway databases

    Reactomei REACT_199398. Cyclin A/B1 associated events during G2/M transition.
    REACT_204375. Cyclin A:Cdk2-associated events at S phase entry.
    REACT_210479. p53-Independent DNA Damage Response.
    REACT_217024. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_218991. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_219991. Cyclin B2 mediated events.

    Miscellaneous databases

    NextBioi 20803681.
    PROi P06652.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view ]
    Pfami PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR00716. MPIPHPHTASE.
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cdc25+ functions as an inducer in the mitotic control of fission yeast."
      Russell P., Nurse P.
      Cell 45:145-153(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99 AND SER-178, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiMPIP_SCHPO
    AccessioniPrimary (citable) accession number: P06652
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3