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Protein

M-phase inducer phosphatase

Gene

cdc25

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate p34(cdc2) and activate the p34(cdc2) kinase activity.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei480By similarity1

GO - Molecular functioni

  • protein tyrosine phosphatase activity Source: PomBase

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic DNA replication checkpoint Source: PomBase
  • positive regulation of G2/MI transition of meiotic cell cycle Source: PomBase
  • positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • regulation of cell size Source: PomBase
  • regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • response to mitotic G2 DNA damage checkpoint signaling Source: PomBase
  • signal transduction involved in intra-S DNA damage checkpoint Source: PomBase

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-SPO-156711. Polo-like kinase mediated events.
R-SPO-176187. Activation of ATR in response to replication stress.
R-SPO-5625740. RHO GTPases activate PKNs.
R-SPO-5689880. Ub-specific processing proteases.
R-SPO-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.
R-SPO-69273. Cyclin A/B1/B2 associated events during G2/M transition.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SPO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase (EC:3.1.3.48)
Alternative name(s):
Mitosis initiation protein
P80
Gene namesi
Name:cdc25
ORF Names:SPAC24H6.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC24H6.05.
PomBaseiSPAC24H6.05. cdc25.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001986611 – 596M-phase inducer phosphataseAdd BLAST596

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei99Phosphoserine1 Publication1
Modified residuei178Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06652.
PRIDEiP06652.

PTM databases

iPTMnetiP06652.

Interactioni

Protein-protein interaction databases

BioGridi279086. 84 interactors.
IntActiP06652. 2 interactors.
MINTiMINT-4686889.
STRINGi4896.SPAC24H6.05.1.

Structurei

3D structure databases

ProteinModelPortaliP06652.
SMRiP06652.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini429 – 533RhodanesePROSITE-ProRule annotationAdd BLAST105

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated

Phylogenomic databases

InParanoidiP06652.
KOiK02555.
OrthoDBiEOG092C2GWG.
PhylomeDBiP06652.

Family and domain databases

CDDicd01530. Cdc25. 1 hit.
Gene3Di3.40.250.10. 1 hit.
InterProiView protein in InterPro
IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
IPR036873. Rhodanese-like_dom_sf.
PfamiView protein in Pfam
PF00581. Rhodanese. 1 hit.
PRINTSiPR00716. MPIPHPHTASE.
SMARTiView protein in SMART
SM00450. RHOD. 1 hit.
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiView protein in PROSITE
PS50206. RHODANESE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P06652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSPLSSLSF TNTLSGKRNV LRPAARELKL MSDRNANQEL DFFFPKSKHI
60 70 80 90 100
ASTLVDPFGK TCSTASPASS LAADMSMNMH IDESPALPTP RRTLFRSLSC
110 120 130 140 150
TVETPLANKT IVSPLPESPS NDALTESYFF RQPASKYSIT QDSPRVSSTI
160 170 180 190 200
AYSFKPKASI ALNTTKSEAT RSSLSSSSFD SYLRPNVSRS RSSGNAPPFL
210 220 230 240 250
RSRSSSSYSI NKKKGTSGGQ ATRHLTYALS RTCSQSSNTT SLLESCLTDD
260 270 280 290 300
TDDFELMSDH EDTFTMGKVA DLPESSVELV EDAASIQRPN SDFGACNDNS
310 320 330 340 350
LDDLFQASPI KPIDMLPKIN KDIAFPSLKV RSPSPMAFAM QEDAEYDEQD
360 370 380 390 400
TPVLRRTQSM FLNSTRLGLF KSQDLVCVTP KQSTKESERF ISSHVEDLSL
410 420 430 440 450
PCFAVKEDSL KRITQETLLG LLDGKFKDIF DKCIIIDCRF EYEYLGGHIS
460 470 480 490 500
TAVNLNTKQA IVDAFLSKPL THRVALVFHC EHSAHRAPHL ALHFRNTDRR
510 520 530 540 550
MNSHRYPFLY YPEVYILHGG YKSFYENHKN RCDPINYVPM NDASHVMTCT
560 570 580 590
KAMNNFKRNA TFMRTKSYTF GQSVLASPDV NDSPTAMHSL STLRRF
Length:596
Mass (Da):66,566
Last modified:November 1, 1995 - v2
Checksum:iCF8D430FC7BD38C1
GO

Sequence cautioni

The sequence AAA35294 differs from that shown. Reason: Frameshift at position 571.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13158 Genomic DNA. Translation: AAA35294.1. Frameshift.
CU329670 Genomic DNA. Translation: CAA90849.1.
PIRiS62407.
RefSeqiNP_592947.1. NM_001018348.2.

Genome annotation databases

EnsemblFungiiSPAC24H6.05.1; SPAC24H6.05.1:pep; SPAC24H6.05.
KEGGispo:SPAC24H6.05.

Similar proteinsi

Entry informationi

Entry nameiMPIP_SCHPO
AccessioniPrimary (citable) accession number: P06652
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1995
Last modified: November 22, 2017
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families