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Protein

M-phase inducer phosphatase

Gene

cdc25

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate p34(cdc2) and activate the p34(cdc2) kinase activity.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei480 – 4801By similarity

GO - Molecular functioni

  1. protein serine/threonine phosphatase activity Source: PomBase
  2. protein tyrosine phosphatase activity Source: PomBase

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. mitotic DNA replication checkpoint Source: PomBase
  3. mitotic nuclear division Source: UniProtKB-KW
  4. peptidyl-tyrosine dephosphorylation involved in activation of protein kinase activity Source: PomBase
  5. positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle Source: PomBase
  6. regulation of cell size Source: PomBase
  7. regulation of G2/M transition of mitotic cell cycle Source: PomBase
  8. signal transduction involved in intra-S DNA damage checkpoint Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_272824. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_284579. Cyclin B2 mediated events.
REACT_285048. p53-Independent DNA Damage Response.
REACT_290054. Cyclin E associated events during G1/S transition.
REACT_291429. Cyclin A/B1 associated events during G2/M transition.
REACT_294541. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_302677. Polo-like kinase mediated events.
REACT_307703. Activation of ATR in response to replication stress.
REACT_308536. Cyclin A:Cdk2-associated events at S phase entry.
REACT_321583. E2F-enabled inhibition of pre-replication complex formation.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase (EC:3.1.3.48)
Alternative name(s):
Mitosis initiation protein
P80
Gene namesi
Name:cdc25
ORF Names:SPAC24H6.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC24H6.05.
PomBaseiSPAC24H6.05.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 596596M-phase inducer phosphatasePRO_0000198661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei99 – 991Phosphoserine1 Publication
Modified residuei178 – 1781Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06652.

Interactioni

Protein-protein interaction databases

BioGridi279086. 83 interactions.
IntActiP06652. 2 interactions.
MINTiMINT-4686889.
STRINGi4896.SPAC24H6.05-1.

Structurei

3D structure databases

ProteinModelPortaliP06652.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini429 – 533105RhodanesePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5105.
InParanoidiP06652.
KOiK02555.
OrthoDBiEOG7VMPGR.
PhylomeDBiP06652.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSPLSSLSF TNTLSGKRNV LRPAARELKL MSDRNANQEL DFFFPKSKHI
60 70 80 90 100
ASTLVDPFGK TCSTASPASS LAADMSMNMH IDESPALPTP RRTLFRSLSC
110 120 130 140 150
TVETPLANKT IVSPLPESPS NDALTESYFF RQPASKYSIT QDSPRVSSTI
160 170 180 190 200
AYSFKPKASI ALNTTKSEAT RSSLSSSSFD SYLRPNVSRS RSSGNAPPFL
210 220 230 240 250
RSRSSSSYSI NKKKGTSGGQ ATRHLTYALS RTCSQSSNTT SLLESCLTDD
260 270 280 290 300
TDDFELMSDH EDTFTMGKVA DLPESSVELV EDAASIQRPN SDFGACNDNS
310 320 330 340 350
LDDLFQASPI KPIDMLPKIN KDIAFPSLKV RSPSPMAFAM QEDAEYDEQD
360 370 380 390 400
TPVLRRTQSM FLNSTRLGLF KSQDLVCVTP KQSTKESERF ISSHVEDLSL
410 420 430 440 450
PCFAVKEDSL KRITQETLLG LLDGKFKDIF DKCIIIDCRF EYEYLGGHIS
460 470 480 490 500
TAVNLNTKQA IVDAFLSKPL THRVALVFHC EHSAHRAPHL ALHFRNTDRR
510 520 530 540 550
MNSHRYPFLY YPEVYILHGG YKSFYENHKN RCDPINYVPM NDASHVMTCT
560 570 580 590
KAMNNFKRNA TFMRTKSYTF GQSVLASPDV NDSPTAMHSL STLRRF
Length:596
Mass (Da):66,566
Last modified:November 1, 1995 - v2
Checksum:iCF8D430FC7BD38C1
GO

Sequence cautioni

The sequence AAA35294.1 differs from that shown. Reason: Frameshift at position 571. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13158 Genomic DNA. Translation: AAA35294.1. Frameshift.
CU329670 Genomic DNA. Translation: CAA90849.1.
PIRiS62407.
RefSeqiNP_592947.1. NM_001018348.2.

Genome annotation databases

EnsemblFungiiSPAC24H6.05.1; SPAC24H6.05.1:pep; SPAC24H6.05.
GeneIDi2542632.
KEGGispo:SPAC24H6.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13158 Genomic DNA. Translation: AAA35294.1. Frameshift.
CU329670 Genomic DNA. Translation: CAA90849.1.
PIRiS62407.
RefSeqiNP_592947.1. NM_001018348.2.

3D structure databases

ProteinModelPortaliP06652.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279086. 83 interactions.
IntActiP06652. 2 interactions.
MINTiMINT-4686889.
STRINGi4896.SPAC24H6.05-1.

Proteomic databases

MaxQBiP06652.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC24H6.05.1; SPAC24H6.05.1:pep; SPAC24H6.05.
GeneIDi2542632.
KEGGispo:SPAC24H6.05.

Organism-specific databases

EuPathDBiFungiDB:SPAC24H6.05.
PomBaseiSPAC24H6.05.

Phylogenomic databases

eggNOGiCOG5105.
InParanoidiP06652.
KOiK02555.
OrthoDBiEOG7VMPGR.
PhylomeDBiP06652.

Enzyme and pathway databases

ReactomeiREACT_272824. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_284579. Cyclin B2 mediated events.
REACT_285048. p53-Independent DNA Damage Response.
REACT_290054. Cyclin E associated events during G1/S transition.
REACT_291429. Cyclin A/B1 associated events during G2/M transition.
REACT_294541. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_302677. Polo-like kinase mediated events.
REACT_307703. Activation of ATR in response to replication stress.
REACT_308536. Cyclin A:Cdk2-associated events at S phase entry.
REACT_321583. E2F-enabled inhibition of pre-replication complex formation.

Miscellaneous databases

NextBioi20803681.
PROiP06652.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cdc25+ functions as an inducer in the mitotic control of fission yeast."
    Russell P., Nurse P.
    Cell 45:145-153(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99 AND SER-178, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiMPIP_SCHPO
AccessioniPrimary (citable) accession number: P06652
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1995
Last modified: April 29, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.