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P06652

- MPIP_SCHPO

UniProt

P06652 - MPIP_SCHPO

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Protein
M-phase inducer phosphatase
Gene
cdc25, SPAC24H6.05
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate p34(cdc2) and activate the p34(cdc2) kinase activity.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei480 – 4801 By similarity

GO - Molecular functioni

  1. protein binding Source: PomBase
  2. protein serine/threonine phosphatase activity Source: PomBase
  3. protein tyrosine phosphatase activity Source: PomBase
Complete GO annotation...

GO - Biological processi

  1. intracellular signal transduction Source: PomBase
  2. mitotic DNA replication checkpoint Source: PomBase
  3. mitotic nuclear division Source: UniProtKB-KW
  4. peptidyl-tyrosine dephosphorylation involved in activation of protein kinase activity Source: PomBase
  5. positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle Source: PomBase
  6. regulation of G2/M transition of mitotic cell cycle Source: PomBase
  7. regulation of cell size Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_199398. Cyclin A/B1 associated events during G2/M transition.
REACT_204375. Cyclin A:Cdk2-associated events at S phase entry.
REACT_210479. p53-Independent DNA Damage Response.
REACT_217024. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_218991. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_219991. Cyclin B2 mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase (EC:3.1.3.48)
Alternative name(s):
Mitosis initiation protein
P80
Gene namesi
Name:cdc25
ORF Names:SPAC24H6.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC24H6.05.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 596596M-phase inducer phosphatase
PRO_0000198661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei99 – 991Phosphoserine1 Publication
Modified residuei178 – 1781Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06652.

Interactioni

Protein-protein interaction databases

BioGridi279086. 76 interactions.
IntActiP06652. 2 interactions.
MINTiMINT-4686889.
STRINGi4896.SPAC24H6.05-1.

Structurei

3D structure databases

ProteinModelPortaliP06652.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini429 – 533105Rhodanese
Add
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.

Phylogenomic databases

eggNOGiCOG5105.
KOiK02555.
OMAiISSHVED.
OrthoDBiEOG7VMPGR.
PhylomeDBiP06652.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06652-1 [UniParc]FASTAAdd to Basket

« Hide

MDSPLSSLSF TNTLSGKRNV LRPAARELKL MSDRNANQEL DFFFPKSKHI    50
ASTLVDPFGK TCSTASPASS LAADMSMNMH IDESPALPTP RRTLFRSLSC 100
TVETPLANKT IVSPLPESPS NDALTESYFF RQPASKYSIT QDSPRVSSTI 150
AYSFKPKASI ALNTTKSEAT RSSLSSSSFD SYLRPNVSRS RSSGNAPPFL 200
RSRSSSSYSI NKKKGTSGGQ ATRHLTYALS RTCSQSSNTT SLLESCLTDD 250
TDDFELMSDH EDTFTMGKVA DLPESSVELV EDAASIQRPN SDFGACNDNS 300
LDDLFQASPI KPIDMLPKIN KDIAFPSLKV RSPSPMAFAM QEDAEYDEQD 350
TPVLRRTQSM FLNSTRLGLF KSQDLVCVTP KQSTKESERF ISSHVEDLSL 400
PCFAVKEDSL KRITQETLLG LLDGKFKDIF DKCIIIDCRF EYEYLGGHIS 450
TAVNLNTKQA IVDAFLSKPL THRVALVFHC EHSAHRAPHL ALHFRNTDRR 500
MNSHRYPFLY YPEVYILHGG YKSFYENHKN RCDPINYVPM NDASHVMTCT 550
KAMNNFKRNA TFMRTKSYTF GQSVLASPDV NDSPTAMHSL STLRRF 596
Length:596
Mass (Da):66,566
Last modified:November 1, 1995 - v2
Checksum:iCF8D430FC7BD38C1
GO

Sequence cautioni

The sequence AAA35294.1 differs from that shown. Reason: Frameshift at position 571.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13158 Genomic DNA. Translation: AAA35294.1. Frameshift.
CU329670 Genomic DNA. Translation: CAA90849.1.
PIRiS62407.
RefSeqiNP_592947.1. NM_001018348.2.

Genome annotation databases

EnsemblFungiiSPAC24H6.05.1; SPAC24H6.05.1:pep; SPAC24H6.05.
GeneIDi2542632.
KEGGispo:SPAC24H6.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13158 Genomic DNA. Translation: AAA35294.1 . Frameshift.
CU329670 Genomic DNA. Translation: CAA90849.1 .
PIRi S62407.
RefSeqi NP_592947.1. NM_001018348.2.

3D structure databases

ProteinModelPortali P06652.
ModBasei Search...

Protein-protein interaction databases

BioGridi 279086. 76 interactions.
IntActi P06652. 2 interactions.
MINTi MINT-4686889.
STRINGi 4896.SPAC24H6.05-1.

Proteomic databases

MaxQBi P06652.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC24H6.05.1 ; SPAC24H6.05.1:pep ; SPAC24H6.05 .
GeneIDi 2542632.
KEGGi spo:SPAC24H6.05.

Organism-specific databases

PomBasei SPAC24H6.05.

Phylogenomic databases

eggNOGi COG5105.
KOi K02555.
OMAi ISSHVED.
OrthoDBi EOG7VMPGR.
PhylomeDBi P06652.

Enzyme and pathway databases

Reactomei REACT_199398. Cyclin A/B1 associated events during G2/M transition.
REACT_204375. Cyclin A:Cdk2-associated events at S phase entry.
REACT_210479. p53-Independent DNA Damage Response.
REACT_217024. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_218991. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_219991. Cyclin B2 mediated events.

Miscellaneous databases

NextBioi 20803681.
PROi P06652.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view ]
Pfami PF00581. Rhodanese. 1 hit.
[Graphical view ]
PRINTSi PR00716. MPIPHPHTASE.
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cdc25+ functions as an inducer in the mitotic control of fission yeast."
    Russell P., Nurse P.
    Cell 45:145-153(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99 AND SER-178, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiMPIP_SCHPO
AccessioniPrimary (citable) accession number: P06652
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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