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Protein

Imidazoleglycerol-phosphate dehydratase

Gene

HIS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (HIS1)
  2. Histidine biosynthesis trifunctional protein (HIS4)
  3. Histidine biosynthesis trifunctional protein (HIS4)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (HIS6)
  5. Imidazole glycerol phosphate synthase hisHF (HIS7)
  6. Imidazoleglycerol-phosphate dehydratase (HIS3)
  7. Histidinol-phosphate aminotransferase (HIS5)
  8. Histidinol-phosphatase (HIS2)
  9. Histidine biosynthesis trifunctional protein (HIS4)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • imidazoleglycerol-phosphate dehydratase activity Source: SGD

GO - Biological processi

  • histidine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YOR202W-MONOMER.
UniPathwayiUPA00031; UER00011.

Names & Taxonomyi

Protein namesi
Recommended name:
Imidazoleglycerol-phosphate dehydratase (EC:4.2.1.19)
Short name:
IGPD
Gene namesi
Name:HIS3
Ordered Locus Names:YOR202W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR202W.
SGDiS000005728. HIS3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Imidazoleglycerol-phosphate dehydratasePRO_0000158250Add
BLAST

Proteomic databases

PRIDEiP06633.

Interactioni

Protein-protein interaction databases

BioGridi34598. 39 interactions.
DIPiDIP-6759N.
IntActiP06633. 8 interactions.
MINTiMINT-407514.

Structurei

3D structure databases

ProteinModelPortaliP06633.
SMRiP06633. Positions 2-205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP06633.
KOiK01693.
OMAiHHIAESC.
OrthoDBiEOG712V6Z.

Family and domain databases

HAMAPiMF_00076. HisB.
InterProiIPR000807. ImidazoleglycerolP_deHydtase.
IPR020565. ImidazoleglycerP_deHydtase_CS.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR23133:SF2. PTHR23133:SF2. 2 hits.
PfamiPF00475. IGPD. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 2 hits.
PROSITEiPS00954. IGP_DEHYDRATASE_1. 1 hit.
PS00955. IGP_DEHYDRATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06633-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQKALVKR ITNETKIQIA ISLKGGPLAI EHSIFPEKEA EAVAEQATQS
60 70 80 90 100
QVINVHTGIG FLDHMIHALA KHSGWSLIVE CIGDLHIDDH HTTEDCGIAL
110 120 130 140 150
GQAFKEALGA VRGVKRFGSG FAPLDEALSR AVVDLSNRPY AVVELGLQRE
160 170 180 190 200
KVGDLSCEMI PHFLESFAEA SRITLHVDCL RGKNDHHRSE SAFKALAVAI
210 220
REATSPNGTN DVPSTKGVLM
Length:220
Mass (Da):23,833
Last modified:November 1, 1997 - v2
Checksum:iC50AC3B3554A9554
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1113GAV → LA (PubMed:3001645).Curated
Sequence conflicti109 – 1113GAV → LA (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti8 – 81V → I in strain: CLIB 219. 1 Publication
Natural varianti30 – 301I → L in strain: CLIB 219, CLIB 410, CLIB 413 and YIIc17 haplotype Ha2. 1 Publication
Natural varianti92 – 921T → A in strain: YIIc12 and YIIc17 haplotype Ha1. 1 Publication
Natural varianti216 – 2161K → N in strain: YIIc17 haplotype Ha2. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03245 Genomic DNA. Translation: CAA27003.1.
U89927 Genomic DNA. Translation: AAB64382.1.
U89928 Genomic DNA. Translation: AAB64385.1.
AJ585705 Genomic DNA. Translation: CAE52225.1.
AJ585706 Genomic DNA. Translation: CAE52226.1.
AJ585707 Genomic DNA. Translation: CAE52227.1.
AJ585708 Genomic DNA. Translation: CAE52228.1.
AJ585709 Genomic DNA. Translation: CAE52229.1.
AJ585710 Genomic DNA. Translation: CAE52230.1.
AJ585711 Genomic DNA. Translation: CAE52231.1.
AJ585712 Genomic DNA. Translation: CAE52232.1.
AJ585713 Genomic DNA. Translation: CAE52233.1.
AJ585714 Genomic DNA. Translation: CAE52234.1.
AJ585715 Genomic DNA. Translation: CAE52235.1.
AJ585716 Genomic DNA. Translation: CAE52236.1.
AJ585717 Genomic DNA. Translation: CAE52237.1.
AJ585718 Genomic DNA. Translation: CAE52238.1.
AJ585719 Genomic DNA. Translation: CAE52239.1.
Z75110 Genomic DNA. Translation: CAA99417.1.
J01330 Genomic DNA. Translation: AAA88723.1.
BK006948 Genomic DNA. Translation: DAA10975.1.
PIRiS67094. DWBYH.
RefSeqiNP_014845.1. NM_001183621.1.

Genome annotation databases

EnsemblFungiiYOR202W; YOR202W; YOR202W.
GeneIDi854377.
KEGGisce:YOR202W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03245 Genomic DNA. Translation: CAA27003.1.
U89927 Genomic DNA. Translation: AAB64382.1.
U89928 Genomic DNA. Translation: AAB64385.1.
AJ585705 Genomic DNA. Translation: CAE52225.1.
AJ585706 Genomic DNA. Translation: CAE52226.1.
AJ585707 Genomic DNA. Translation: CAE52227.1.
AJ585708 Genomic DNA. Translation: CAE52228.1.
AJ585709 Genomic DNA. Translation: CAE52229.1.
AJ585710 Genomic DNA. Translation: CAE52230.1.
AJ585711 Genomic DNA. Translation: CAE52231.1.
AJ585712 Genomic DNA. Translation: CAE52232.1.
AJ585713 Genomic DNA. Translation: CAE52233.1.
AJ585714 Genomic DNA. Translation: CAE52234.1.
AJ585715 Genomic DNA. Translation: CAE52235.1.
AJ585716 Genomic DNA. Translation: CAE52236.1.
AJ585717 Genomic DNA. Translation: CAE52237.1.
AJ585718 Genomic DNA. Translation: CAE52238.1.
AJ585719 Genomic DNA. Translation: CAE52239.1.
Z75110 Genomic DNA. Translation: CAA99417.1.
J01330 Genomic DNA. Translation: AAA88723.1.
BK006948 Genomic DNA. Translation: DAA10975.1.
PIRiS67094. DWBYH.
RefSeqiNP_014845.1. NM_001183621.1.

3D structure databases

ProteinModelPortaliP06633.
SMRiP06633. Positions 2-205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34598. 39 interactions.
DIPiDIP-6759N.
IntActiP06633. 8 interactions.
MINTiMINT-407514.

Proteomic databases

PRIDEiP06633.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR202W; YOR202W; YOR202W.
GeneIDi854377.
KEGGisce:YOR202W.

Organism-specific databases

EuPathDBiFungiDB:YOR202W.
SGDiS000005728. HIS3.

Phylogenomic databases

InParanoidiP06633.
KOiK01693.
OMAiHHIAESC.
OrthoDBiEOG712V6Z.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00011.
BioCyciYEAST:YOR202W-MONOMER.

Miscellaneous databases

PROiP06633.

Family and domain databases

HAMAPiMF_00076. HisB.
InterProiIPR000807. ImidazoleglycerolP_deHydtase.
IPR020565. ImidazoleglycerP_deHydtase_CS.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR23133:SF2. PTHR23133:SF2. 2 hits.
PfamiPF00475. IGPD. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 2 hits.
PROSITEiPS00954. IGP_DEHYDRATASE_1. 1 hit.
PS00955. IGP_DEHYDRATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and transcriptional mapping of the yeast pet56-his3-ded1 gene region."
    Struhl K.
    Nucleic Acids Res. 13:8587-8601(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "pHISi-1, complete sequence."
    Holtz A., Lou Y.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny."
    Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., Souciet J.-L.
    Nucleic Acids Res. 32:2069-2078(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-8; LEU-30; ALA-92 AND ASN-216.
    Strain: CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12 and YIIc17.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Promotor mutants of the yeast his3 gene."
    Struhl K., Davis R.W.
    J. Mol. Biol. 152:553-568(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHIS7_YEAST
AccessioniPrimary (citable) accession number: P06633
Secondary accession number(s): D6W2Q9
, Q12695, Q70D78, Q70D79, Q70D81, Q70D83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.