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Protein

Imidazoleglycerol-phosphate dehydratase

Gene

HIS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 2100 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (HIS1)
  2. Histidine biosynthesis trifunctional protein (HIS4)
  3. Histidine biosynthesis trifunctional protein (HIS4)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (HIS6)
  5. Imidazole glycerol phosphate synthase hisHF (HIS7)
  6. Imidazoleglycerol-phosphate dehydratase (HIS3)
  7. Histidinol-phosphate aminotransferase (HIS5)
  8. Histidinol-phosphatase (HIS2)
  9. Histidine biosynthesis trifunctional protein (HIS4)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • imidazoleglycerol-phosphate dehydratase activity Source: SGD

GO - Biological processi

  • histidine biosynthetic process Source: SGD

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YOR202W-MONOMER
UniPathwayiUPA00031; UER00011

Names & Taxonomyi

Protein namesi
Recommended name:
Imidazoleglycerol-phosphate dehydratase (EC:4.2.1.19)
Short name:
IGPD
Gene namesi
Name:HIS3
Ordered Locus Names:YOR202W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR202W
SGDiS000005728 HIS3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001582501 – 220Imidazoleglycerol-phosphate dehydrataseAdd BLAST220

Proteomic databases

PaxDbiP06633
PRIDEiP06633

Interactioni

Protein-protein interaction databases

BioGridi34598, 44 interactors
DIPiDIP-6759N
IntActiP06633, 8 interactors
MINTiP06633
STRINGi4932.YOR202W

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 8Combined sources4
Beta strandi13 – 22Combined sources10
Beta strandi34 – 36Combined sources3
Beta strandi45 – 47Combined sources3
Beta strandi49 – 56Combined sources8
Helixi60 – 71Combined sources12
Turni72 – 74Combined sources3
Beta strandi76 – 83Combined sources8
Helixi91 – 107Combined sources17
Beta strandi118 – 124Combined sources7
Beta strandi127 – 133Combined sources7
Beta strandi140 – 144Combined sources5
Beta strandi152 – 155Combined sources4
Helixi159 – 171Combined sources13
Beta strandi174 – 181Combined sources8
Helixi185 – 203Combined sources19
Beta strandi215 – 217Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6EZMelectron microscopy3.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-220[»]
ProteinModelPortaliP06633
SMRiP06633
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP06633
KOiK01693
OMAiARHGLFD
OrthoDBiEOG092C4Y0M

Family and domain databases

CDDicd07914 IGPD, 1 hit
Gene3Di3.30.230.40, 2 hits
HAMAPiMF_00076 HisB, 1 hit
InterProiView protein in InterPro
IPR038494 IGPD_sf
IPR000807 ImidazoleglycerolP_deHydtase
IPR020565 ImidazoleglycerP_deHydtase_CS
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR23133 PTHR23133, 1 hit
PfamiView protein in Pfam
PF00475 IGPD, 1 hit
SUPFAMiSSF54211 SSF54211, 2 hits
PROSITEiView protein in PROSITE
PS00954 IGP_DEHYDRATASE_1, 1 hit
PS00955 IGP_DEHYDRATASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P06633-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQKALVKR ITNETKIQIA ISLKGGPLAI EHSIFPEKEA EAVAEQATQS
60 70 80 90 100
QVINVHTGIG FLDHMIHALA KHSGWSLIVE CIGDLHIDDH HTTEDCGIAL
110 120 130 140 150
GQAFKEALGA VRGVKRFGSG FAPLDEALSR AVVDLSNRPY AVVELGLQRE
160 170 180 190 200
KVGDLSCEMI PHFLESFAEA SRITLHVDCL RGKNDHHRSE SAFKALAVAI
210 220
REATSPNGTN DVPSTKGVLM
Length:220
Mass (Da):23,833
Last modified:November 1, 1997 - v2
Checksum:iC50AC3B3554A9554
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109 – 111GAV → LA (PubMed:3001645).Curated3
Sequence conflicti109 – 111GAV → LA (Ref. 2) Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti8V → I in strain: CLIB 219. 1 Publication1
Natural varianti30I → L in strain: CLIB 219, CLIB 410, CLIB 413 and YIIc17 haplotype Ha2. 1 Publication1
Natural varianti92T → A in strain: YIIc12 and YIIc17 haplotype Ha1. 1 Publication1
Natural varianti216K → N in strain: YIIc17 haplotype Ha2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03245 Genomic DNA Translation: CAA27003.1
U89927 Genomic DNA Translation: AAB64382.1
U89928 Genomic DNA Translation: AAB64385.1
AJ585705 Genomic DNA Translation: CAE52225.1
AJ585706 Genomic DNA Translation: CAE52226.1
AJ585707 Genomic DNA Translation: CAE52227.1
AJ585708 Genomic DNA Translation: CAE52228.1
AJ585709 Genomic DNA Translation: CAE52229.1
AJ585710 Genomic DNA Translation: CAE52230.1
AJ585711 Genomic DNA Translation: CAE52231.1
AJ585712 Genomic DNA Translation: CAE52232.1
AJ585713 Genomic DNA Translation: CAE52233.1
AJ585714 Genomic DNA Translation: CAE52234.1
AJ585715 Genomic DNA Translation: CAE52235.1
AJ585716 Genomic DNA Translation: CAE52236.1
AJ585717 Genomic DNA Translation: CAE52237.1
AJ585718 Genomic DNA Translation: CAE52238.1
AJ585719 Genomic DNA Translation: CAE52239.1
Z75110 Genomic DNA Translation: CAA99417.1
J01330 Genomic DNA Translation: AAA88723.1
BK006948 Genomic DNA Translation: DAA10975.1
PIRiS67094 DWBYH
RefSeqiNP_014845.1, NM_001183621.1

Genome annotation databases

EnsemblFungiiYOR202W; YOR202W; YOR202W
GeneIDi854377
KEGGisce:YOR202W

Similar proteinsi

Entry informationi

Entry nameiHIS7_YEAST
AccessioniPrimary (citable) accession number: P06633
Secondary accession number(s): D6W2Q9
, Q12695, Q70D78, Q70D79, Q70D81, Q70D83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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