Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sporulation initiation phosphotransferase F

Gene

spo0F

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Magnesium
Metal bindingi11 – 111Magnesium
Metal bindingi54 – 541Magnesium
Metal bindingi56 – 561Magnesium; via carbonyl oxygen

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Sporulation, Two-component regulatory system

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU37130-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation initiation phosphotransferase F (EC:2.7.-.-)
Alternative name(s):
Stage 0 sporulation protein F
Gene namesi
Name:spo0F
Ordered Locus Names:BSU37130
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2111331.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 124124Sporulation initiation phosphotransferase FPRO_0000081244Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei54 – 5414-aspartylphosphatePROSITE-ProRule annotation1 Publication

Post-translational modificationi

Phosphorylated by KinA and KinB. Dephosphorylated by RapA and RapB.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP06628.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
kinAP164972EBI-6418009,EBI-6405707

Protein-protein interaction databases

DIPiDIP-58965N.
IntActiP06628. 3 interactions.
STRINGi224308.Bsubs1_010100020071.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Helixi13 – 2412Combined sources
Turni25 – 273Combined sources
Beta strandi29 – 357Combined sources
Helixi36 – 4611Combined sources
Beta strandi49 – 557Combined sources
Beta strandi58 – 603Combined sources
Helixi62 – 7211Combined sources
Beta strandi77 – 848Combined sources
Helixi87 – 9610Combined sources
Beta strandi101 – 1055Combined sources
Helixi108 – 11811Combined sources
Beta strandi122 – 1243Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F51X-ray3.00E/F/G/H3-121[»]
1FSPNMR-A1-124[»]
1NATX-ray2.45A1-124[»]
1PEYX-ray2.25A/B/C1-124[»]
1PUXNMR-A1-124[»]
1SRRX-ray1.90A/B/C1-124[»]
2FSPNMR-A1-124[»]
2FTKX-ray3.05E/F/G/H1-124[»]
2JVINMR-A1-124[»]
2JVJNMR-A1-124[»]
2JVKNMR-A1-124[»]
3Q15X-ray2.19C/D1-124[»]
ProteinModelPortaliP06628.
SMRiP06628. Positions 4-119.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06628.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 119115Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410812K. Bacteria.
COG0784. LUCA.
HOGENOMiHOG000034820.
InParanoidiP06628.
KOiK02490.
OMAiEVFQREG.
PhylomeDBiP06628.

Family and domain databases

InterProiIPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNEKILIVD DQYGIRILLN EVFNKEGYQT FQAANGLQAL DIVTKERPDL
60 70 80 90 100
VLLDMKIPGM DGIEILKRMK VIDENIRVII MTAYGELDMI QESKELGALT
110 120
HFAKPFDIDE IRDAVKKYLP LKSN
Length:124
Mass (Da):14,228
Last modified:January 1, 1988 - v1
Checksum:i4130377E3558D698
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03497 Genomic DNA. Translation: CAA27217.1.
M11081 Genomic DNA. Translation: AAA22787.1.
M22039 Unassigned DNA. Translation: AAA16802.1.
Z49782 Genomic DNA. Translation: CAA89872.1.
AL009126 Genomic DNA. Translation: CAB15730.1.
PIRiA24737. SZBS0F.
RefSeqiNP_391594.1. NC_000964.3.
WP_003227621.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15730; CAB15730; BSU37130.
GeneIDi937041.
KEGGibsu:BSU37130.
PATRICi18979462. VBIBacSub10457_3893.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03497 Genomic DNA. Translation: CAA27217.1.
M11081 Genomic DNA. Translation: AAA22787.1.
M22039 Unassigned DNA. Translation: AAA16802.1.
Z49782 Genomic DNA. Translation: CAA89872.1.
AL009126 Genomic DNA. Translation: CAB15730.1.
PIRiA24737. SZBS0F.
RefSeqiNP_391594.1. NC_000964.3.
WP_003227621.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F51X-ray3.00E/F/G/H3-121[»]
1FSPNMR-A1-124[»]
1NATX-ray2.45A1-124[»]
1PEYX-ray2.25A/B/C1-124[»]
1PUXNMR-A1-124[»]
1SRRX-ray1.90A/B/C1-124[»]
2FSPNMR-A1-124[»]
2FTKX-ray3.05E/F/G/H1-124[»]
2JVINMR-A1-124[»]
2JVJNMR-A1-124[»]
2JVKNMR-A1-124[»]
3Q15X-ray2.19C/D1-124[»]
ProteinModelPortaliP06628.
SMRiP06628. Positions 4-119.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-58965N.
IntActiP06628. 3 interactions.
STRINGi224308.Bsubs1_010100020071.

Chemistry

ChEMBLiCHEMBL2111331.

Proteomic databases

PaxDbiP06628.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15730; CAB15730; BSU37130.
GeneIDi937041.
KEGGibsu:BSU37130.
PATRICi18979462. VBIBacSub10457_3893.

Phylogenomic databases

eggNOGiENOG410812K. Bacteria.
COG0784. LUCA.
HOGENOMiHOG000034820.
InParanoidiP06628.
KOiK02490.
OMAiEVFQREG.
PhylomeDBiP06628.

Enzyme and pathway databases

BioCyciBSUB:BSU37130-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06628.
PROiP06628.

Family and domain databases

InterProiIPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSP0F_BACSU
AccessioniPrimary (citable) accession number: P06628
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.