Reviewed, UniProtKB/Swiss-Prot P06624 (MIP_BOVIN)

Names and origin

Protein names

Recommended name:
    Lens fiber major intrinsic protein
Alternative name(s):
    Aquaporin-0
    MIP26
      Short name=MP26

Gene names

Name:

MIP

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Water channel. May be responsible for regulating the osmolarity of the lens.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Tissue specificity	Major component of lens fiber gap junctions.
Developmental stage	Higher expression in pre-natal (1-5 months gestation) than in postnatal (4-6 months) calf lens.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell junction Cell membrane Eye lens protein Gap junction Membrane
Domain	Repeat Transmembrane
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	gap junction Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	structural constituent of eye lens Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 263

263

Lens fiber major intrinsic protein

PRO_0000063910

Regions

Topological domain

1 – 16

16

Cytoplasmic Potential

Transmembrane

17 – 37

21

Potential

Topological domain

38 – 40

3

Extracellular Potential

Transmembrane

41 – 60

20

Potential

Topological domain

61 – 85

25

Cytoplasmic Potential

Transmembrane

86 – 107

22

Potential

Topological domain

108 – 123

16

Extracellular Potential

Transmembrane

124 – 149

26

Potential

Topological domain

150 – 156

7

Cytoplasmic Potential

Transmembrane

157 – 178

22

Potential

Topological domain

179 – 196

18

Extracellular Potential

Transmembrane

197 – 219

23

Potential

Topological domain

220 – 263

44

Cytoplasmic Potential

Motif

68 – 70

3

NPA 1

Motif

184 – 186

3

NPA 2

Amino acid modifications

Modified residue

235

1

Phosphoserine Ref.4

Modified residue

243

1

Phosphoserine; by PKA Ref.3

Modified residue

245

1

Phosphoserine Ref.3

Modified residue

246

1

Deamidated asparagine Ref.3

Experimental info

Sequence conflict

14

1

C → L AA sequence Ref.2

Sequence conflict

29 – 33

5

GASLR → RAFLL AA sequence Ref.2

Secondary structure

1

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263

Helix Strand Turn

Details...

Helix

10 – 31

22

Helix

38 – 59

22

Turn

60 – 63

4

Helix

69 – 77

9

Helix

83 – 107

25

Turn

110 – 112

3

Turn

114 – 117

4

Helix

127 – 149

23

Helix

160 – 179

20

Helix

185 – 195

11

Turn

199 – 202

4

Helix

203 – 220

18

Turn

221 – 223

3

Helix

230 – 238

9

Beta strand

256 – 258

3

Sequences

	Sequence		Length	Mass (Da)
	P06624-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: E08C2C4F33398D4E	FASTA	263	28,223
	10 20 30 40 50 60 MWELRSASFW RAICAEFFAS LFYVFFGLGA SLRWAPGPLH VLQVALAFGL ALATLVQAVG 70 80 90 100 110 120 HISGAHVNPA VTFAFLVGSQ MSLLRAICYM VAQLLGAVAG AAVLYSVTPP AVRGNLALNT 130 140 150 160 170 180 LHPGVSVGQA TIVEIFLTLQ FVLCIFATYD ERRNGRLGSV ALAVGFSLTL GHLFGMYYTG 190 200 210 220 230 240 AGMNPARSFA PAILTRNFTN HWVYWVGPVI GAGLGSLLYD FLLFPRLKSV SERLSILKGS 250 260 RPSESNGQPE VTGEPVELKT QAL

References

[1]	"The major intrinsic protein (MIP) of the bovine lens fiber membrane: characterization and structure based on cDNA cloning." Gorin M.B., Yancey S.B., Cline J., Revel J.-P., Horwitz J. Cell 39:49-59(1984) [PubMed: 6207938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-39.
[2]	"Sequence analysis of peptide fragments from the intrinsic membrane protein of calf lens fibers MP26 and its natural maturation product MP22." Ngoc L.D., Paroutaud P., Dunia I., Benedetti E.L., Hoebeke J. FEBS Lett. 181:74-78(1985) [PubMed: 3882455] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-33. Tissue: Lens.
[3]	"Amino acid sequence of in vivo phosphorylation sites in the main intrinsic protein (MIP) of lens membranes." Lampe P.D., Johnson R.G. Eur. J. Biochem. 194:541-547(1990) [PubMed: 2176601] [Abstract] Cited for: PROTEIN SEQUENCE OF 239-259, PHOSPHORYLATION AT SER-243 AND SER-245, DEAMIDATION AT ASN-246. Tissue: Lens.
[4]	"Complete map and identification of the phosphorylation site of bovine lens major intrinsic protein." Schey K.L., Fowler J.G., Schwartz J.C., Busman M., Dillon J., Crouch R.K. Invest. Ophthalmol. Vis. Sci. 38:2508-2515(1997) [PubMed: 9375569] [Abstract] Cited for: PHOSPHORYLATION AT SER-235. Tissue: Lens.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K02818 mRNA. Translation: AAA30622.1.

IPI

IPI00699453.

PIR

MMBOLM. A23251.

RefSeq

NP_776362.1.

UniGene

Bt.4516

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YMG	X-ray	2.24	A	1-263	[»]
2B6P	X-ray	2.40	A	1-263	[»]
2C32	X-ray	7.01	A	1-263	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P06624.

Protein family/group databases

TCDB

1.A.8.8.2. major intrinsic protein (MIP) family.

Genome annotation databases

Ensembl

ENSBTAT00000013360; ENSBTAP00000013360; ENSBTAG00000010127; Bos taurus. [Genome view]

GeneID

280859.

KEGG

bta:280859.

Organism-specific databases

CTD

280859.

Phylogenomic databases

eggNOG

maNOG10666.

HOVERGEN

P06624.

InParanoid

P06624.

OMA

LVQTVGH.

PhylomeDB

P06624.

Family and domain databases

InterPro

IPR012269. Aquaporin.
IPR000425. MIP.
[Graphical view]

Gene3D

G3DSA:1.20.1080.10. MIP. 1 hit.

PANTHER

PTHR19139. MIP. 1 hit.

Pfam

PF00230. MIP. 1 hit.
[Graphical view]

PRINTS

PR00783. MINTRINSICP.

TIGRFAMs

TIGR00861. MIP. 1 hit.

PROSITE

PS00221. MIP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MIP_BOVIN

Accession

Primary (citable) accession number: P06624

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	February 9, 2010
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)