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Protein

Metapyrocatechase

Gene

xylE

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Catechol + O2 = 2-hydroxymuconate-6-semialdehyde.

Cofactori

Pathwayi: toluene degradation

This protein is involved in the pathway toluene degradation, which is part of Xenobiotic degradation.
View all proteins of this organism that are known to be involved in the pathway toluene degradation and in Xenobiotic degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi153Iron1
Metal bindingi214Iron1
Metal bindingi265Iron1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3421.
BRENDAi1.13.11.2. 5092.
UniPathwayiUPA00273.

Names & Taxonomyi

Protein namesi
Recommended name:
Metapyrocatechase (EC:1.13.11.2)
Short name:
MPC
Alternative name(s):
CatO2ase
Catechol 2,3-dioxygenase
Gene namesi
Name:xylE
Encoded oniPlasmid TOL pWW00 Publication
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000850271 – 307MetapyrocatechaseAdd BLAST307

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1307
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 16Combined sources13
Helixi18 – 27Combined sources10
Beta strandi32 – 36Combined sources5
Beta strandi42 – 45Combined sources4
Beta strandi54 – 59Combined sources6
Beta strandi64 – 73Combined sources10
Helixi75 – 88Combined sources14
Beta strandi93 – 95Combined sources3
Beta strandi106 – 110Combined sources5
Beta strandi116 – 121Combined sources6
Beta strandi133 – 135Combined sources3
Beta strandi150 – 159Combined sources10
Helixi161 – 170Combined sources10
Beta strandi175 – 181Combined sources7
Beta strandi187 – 198Combined sources12
Beta strandi200 – 205Combined sources6
Beta strandi207 – 218Combined sources12
Helixi222 – 235Combined sources14
Beta strandi239 – 245Combined sources7
Beta strandi252 – 257Combined sources6
Beta strandi263 – 268Combined sources6
Beta strandi280 – 283Combined sources4
Helixi284 – 286Combined sources3
Helixi287 – 291Combined sources5
Turni293 – 295Combined sources3
Helixi300 – 303Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MPYX-ray2.80A/B/C/D1-307[»]
ProteinModelPortaliP06622.
SMRiP06622.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06622.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.10.180.10. 2 hits.
InterProiIPR017624. Catechol_2-3_dOase.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR000486. Xdiol_ring_cleave_dOase_1/2.
[Graphical view]
PfamiPF00903. Glyoxalase. 2 hits.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR03211. catechol_2_3. 1 hit.
PROSITEiPS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKGVMRPGH VQLRVLDMSK ALEHYVELLG LIEMDRDDQG RVYLKAWTEV
60 70 80 90 100
DKFSLVLREA DEPGMDFMGF KVVDEDALRQ LERDLMAYGC AVEQLPAGEL
110 120 130 140 150
NSCGRRVRFQ APSGHHFELY ADKEYTGKWG LNDVNPEAWP RDLKGMAAVR
160 170 180 190 200
FDHALMYGDE LPATYDLFTK VLGFYLAEQV LDENGTRVAQ FLSLSTKAHD
210 220 230 240 250
VAFIHHPEKG RLHHVSFHLE TWEDLLRAAD LISMTDTSID IGPTRHGLTH
260 270 280 290 300
GKTIYFFDPS GNRNEVFCGG DYNYPDHKPV TWTTDQLGKA IFYHDRILNE

RFMTVLT
Length:307
Mass (Da):35,156
Last modified:January 1, 1988 - v1
Checksum:i80F189CF33554FAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01161 Genomic DNA. Translation: CAA24490.1.
M64747 Genomic DNA. Translation: AAA26052.1.
PIRiA20852.
RefSeqiNP_542866.1. NC_003350.1.
WP_011005909.1. NC_003350.1.

Genome annotation databases

GeneIDi1218746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01161 Genomic DNA. Translation: CAA24490.1.
M64747 Genomic DNA. Translation: AAA26052.1.
PIRiA20852.
RefSeqiNP_542866.1. NC_003350.1.
WP_011005909.1. NC_003350.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MPYX-ray2.80A/B/C/D1-307[»]
ProteinModelPortaliP06622.
SMRiP06622.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1218746.

Enzyme and pathway databases

UniPathwayiUPA00273.
BioCyciMetaCyc:MONOMER-3421.
BRENDAi1.13.11.2. 5092.

Miscellaneous databases

EvolutionaryTraceiP06622.

Family and domain databases

Gene3Di3.10.180.10. 2 hits.
InterProiIPR017624. Catechol_2-3_dOase.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR000486. Xdiol_ring_cleave_dOase_1/2.
[Graphical view]
PfamiPF00903. Glyoxalase. 2 hits.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR03211. catechol_2_3. 1 hit.
PROSITEiPS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXYLE1_PSEPU
AccessioniPrimary (citable) accession number: P06622
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.