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P06622

- XYLE1_PSEPU

UniProt

P06622 - XYLE1_PSEPU

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Protein

Metapyrocatechase

Gene

xylE

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Catechol + O2 = 2-hydroxymuconate-6-semialdehyde.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi153 – 1531Iron
Metal bindingi214 – 2141Iron
Metal bindingi265 – 2651Iron

GO - Molecular functioni

  1. catechol 2,3-dioxygenase activity Source: UniProtKB-EC
  2. ferrous iron binding Source: InterPro

GO - Biological processi

  1. toluene catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3421.
UniPathwayiUPA00273.

Names & Taxonomyi

Protein namesi
Recommended name:
Metapyrocatechase (EC:1.13.11.2)
Short name:
MPC
Alternative name(s):
CatO2ase
Catechol 2,3-dioxygenase
Gene namesi
Name:xylE
Encoded oniPlasmid TOL pWW00 Publication
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307MetapyrocatechasePRO_0000085027Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1
307
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 1613Combined sources
Helixi18 – 2710Combined sources
Beta strandi32 – 365Combined sources
Beta strandi42 – 454Combined sources
Beta strandi54 – 596Combined sources
Beta strandi64 – 7310Combined sources
Helixi75 – 8814Combined sources
Beta strandi93 – 953Combined sources
Beta strandi106 – 1105Combined sources
Beta strandi116 – 1216Combined sources
Beta strandi133 – 1353Combined sources
Beta strandi150 – 15910Combined sources
Helixi161 – 17010Combined sources
Beta strandi175 – 1817Combined sources
Beta strandi187 – 19812Combined sources
Beta strandi200 – 2056Combined sources
Beta strandi207 – 21812Combined sources
Helixi222 – 23514Combined sources
Beta strandi239 – 2457Combined sources
Beta strandi252 – 2576Combined sources
Beta strandi263 – 2686Combined sources
Beta strandi280 – 2834Combined sources
Helixi284 – 2863Combined sources
Helixi287 – 2915Combined sources
Turni293 – 2953Combined sources
Helixi300 – 3034Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MPYX-ray2.80A/B/C/D1-307[»]
ProteinModelPortaliP06622.
SMRiP06622. Positions 1-307.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06622.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.10.180.10. 2 hits.
InterProiIPR017624. Catechol_2-3_dOase.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR000486. Xdiol_ring_cleave_dOase_1/2.
[Graphical view]
PfamiPF00903. Glyoxalase. 2 hits.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR03211. catechol_2_3. 1 hit.
PROSITEiPS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06622-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKGVMRPGH VQLRVLDMSK ALEHYVELLG LIEMDRDDQG RVYLKAWTEV
60 70 80 90 100
DKFSLVLREA DEPGMDFMGF KVVDEDALRQ LERDLMAYGC AVEQLPAGEL
110 120 130 140 150
NSCGRRVRFQ APSGHHFELY ADKEYTGKWG LNDVNPEAWP RDLKGMAAVR
160 170 180 190 200
FDHALMYGDE LPATYDLFTK VLGFYLAEQV LDENGTRVAQ FLSLSTKAHD
210 220 230 240 250
VAFIHHPEKG RLHHVSFHLE TWEDLLRAAD LISMTDTSID IGPTRHGLTH
260 270 280 290 300
GKTIYFFDPS GNRNEVFCGG DYNYPDHKPV TWTTDQLGKA IFYHDRILNE

RFMTVLT
Length:307
Mass (Da):35,156
Last modified:January 1, 1988 - v1
Checksum:i80F189CF33554FAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01161 Genomic DNA. Translation: CAA24490.1.
M64747 Genomic DNA. Translation: AAA26052.1.
PIRiA20852.
RefSeqiNP_542866.1. NC_003350.1.
WP_011005909.1. NC_003350.1.

Genome annotation databases

GeneIDi1218746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01161 Genomic DNA. Translation: CAA24490.1 .
M64747 Genomic DNA. Translation: AAA26052.1 .
PIRi A20852.
RefSeqi NP_542866.1. NC_003350.1.
WP_011005909.1. NC_003350.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MPY X-ray 2.80 A/B/C/D 1-307 [» ]
ProteinModelPortali P06622.
SMRi P06622. Positions 1-307.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1218746.

Enzyme and pathway databases

UniPathwayi UPA00273 .
BioCyci MetaCyc:MONOMER-3421.

Miscellaneous databases

EvolutionaryTracei P06622.

Family and domain databases

Gene3Di 3.10.180.10. 2 hits.
InterProi IPR017624. Catechol_2-3_dOase.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR000486. Xdiol_ring_cleave_dOase_1/2.
[Graphical view ]
Pfami PF00903. Glyoxalase. 2 hits.
[Graphical view ]
SUPFAMi SSF54593. SSF54593. 1 hit.
TIGRFAMsi TIGR03211. catechol_2_3. 1 hit.
PROSITEi PS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of the metapyrocatechase gene on the TOL plasmid of Pseudomonas putida mt-2."
    Nakai C., Kagamiyama H., Nozaki M., Nakzawa T., Inouye S., Ebina Y., Nakazawa A.
    J. Biol. Chem. 258:2923-2928(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2.
  2. "Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene."
    Zukowski M.M., Gaffney D.F., Speck D., Kauffmann M., Findeli A., Wisecup A., Lecocq J.-P.
    Proc. Natl. Acad. Sci. U.S.A. 80:1101-1105(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Zukowski M.M.
    Submitted (MAY-1983) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. "Cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily."
    Neidle E.L., Hartnett C., Ornston L.N., Bairoch A., Rekik M., Harayama S.
    Eur. J. Biochem. 204:113-120(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "DNA sequence determination of the TOL plasmid (pWWO) xylGFJ genes of Pseudomonas putida: implications for the evolution of aromatic catabolism."
    Horn J.M., Harayama S., Timmis K.N.
    Mol. Microbiol. 5:2459-2474(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 284-307.
  6. "An archetypical extradiol-cleaving catecholic dioxygenase: the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Ppseudomonas putida mt-2."
    Kita A., Kita S., Fujisawa I., Inaka K., Ishida T., Horiike K., Nozaki M., Miki K.
    Structure 7:25-34(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

Entry informationi

Entry nameiXYLE1_PSEPU
AccessioniPrimary (citable) accession number: P06622
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 26, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3