Reviewed, UniProtKB/Swiss-Prot P06622 (XYLE1_PSEPU)

Names and origin

Protein names

Recommended name:
    Metapyrocatechase
      Short name=MPC
    EC=1.13.11.2
Alternative name(s):
    CatO2ase
    Catechol 2,3-dioxygenase

Gene names

Name:

xylE

Encoded on

Plasmid TOL pWW0

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Catechol + O2 = 2-hydroxymuconate semialdehyde.
Cofactor	Fe2+ ion.
Pathway	Xenobiotic degradation; toluene degradation.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the extradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 307

307

Metapyrocatechase

PRO_0000085027

Sites

Metal binding

153

1

Iron

Metal binding

214

1

Iron

Metal binding

265

1

Iron

Secondary structure

1

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307

Helix Strand Turn

Details...

Beta strand

4 – 16

13

Helix

18 – 27

10

Beta strand

32 – 36

5

Beta strand

42 – 45

4

Beta strand

54 – 59

6

Beta strand

64 – 73

10

Helix

75 – 88

14

Beta strand

93 – 95

3

Beta strand

106 – 110

5

Beta strand

116 – 121

6

Beta strand

133 – 135

3

Beta strand

150 – 159

10

Helix

161 – 170

10

Beta strand

175 – 181

7

Beta strand

187 – 198

12

Beta strand

200 – 205

6

Beta strand

207 – 218

12

Helix

222 – 235

14

Beta strand

239 – 245

7

Beta strand

252 – 257

6

Beta strand

263 – 268

6

Beta strand

280 – 283

4

Helix

284 – 286

3

Helix

287 – 291

5

Turn

293 – 295

3

Helix

300 – 303

4

Sequences

	Sequence		Length	Mass (Da)
	P06622-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 80F189CF33554FAA	FASTA	307	35,156
	10 20 30 40 50 60 MNKGVMRPGH VQLRVLDMSK ALEHYVELLG LIEMDRDDQG RVYLKAWTEV DKFSLVLREA 70 80 90 100 110 120 DEPGMDFMGF KVVDEDALRQ LERDLMAYGC AVEQLPAGEL NSCGRRVRFQ APSGHHFELY 130 140 150 160 170 180 ADKEYTGKWG LNDVNPEAWP RDLKGMAAVR FDHALMYGDE LPATYDLFTK VLGFYLAEQV 190 200 210 220 230 240 LDENGTRVAQ FLSLSTKAHD VAFIHHPEKG RLHHVSFHLE TWEDLLRAAD LISMTDTSID 250 260 270 280 290 300 IGPTRHGLTH GKTIYFFDPS GNRNEVFCGG DYNYPDHKPV TWTTDQLGKA IFYHDRILNE RFMTVLT

References

[1]	"Complete nucleotide sequence of the metapyrocatechase gene on the TOL plasmid of Pseudomonas putida mt-2." Nakai C., Kagamiyama H., Nozaki M., Nakzawa T., Inouye S., Ebina Y., Nakazawa A. J. Biol. Chem. 258:2923-2928(1983) [PubMed: 6826546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33015 / mt-2 / JCM 6156.
[2]	"Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene." Zukowski M.M., Gaffney D.F., Speck D., Kauffmann M., Findeli A., Wisecup A., Lecocq J.-P. Proc. Natl. Acad. Sci. U.S.A. 80:1101-1105(1983) [PubMed: 6405380] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Zukowski M.M. Submitted (MAY-1983) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"Cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily." Neidle E.L., Hartnett C., Ornston L.N., Bairoch A., Rekik M., Harayama S. Eur. J. Biochem. 204:113-120(1992) [PubMed: 1740120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"DNA sequence determination of the TOL plasmid (pWWO) xylGFJ genes of Pseudomonas putida: implications for the evolution of aromatic catabolism." Horn J.M., Harayama S., Timmis K.N. Mol. Microbiol. 5:2459-2474(1991) [PubMed: 1791759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 284-307.
[6]	"An archetypical extradiol-cleaving catecholic dioxygenase: the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Ppseudomonas putida mt-2." Kita A., Kita S., Fujisawa I., Inaka K., Ishida T., Horiike K., Nozaki M., Miki K. Structure 7:25-34(1999) [PubMed: 10368270] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

V01161 Genomic DNA. Translation: CAA24490.1.
M64747 Genomic DNA. Translation: AAA26052.1.

PIR

A20852.

RefSeq

NP_542866.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MPY	X-ray	2.80	A/B/C/D	1-307	[»]

ModBase

Search...

Genome annotation databases

GeneID

1218746.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-3421.

BRENDA

1.13.11.2. 403.

Family and domain databases

InterPro

IPR017624. Catechol_2-3_dOase.
IPR004360. Glyas_bleo-R_dOase.
IPR000486. Xdiol_dOase_1_2.
[Graphical view]

Pfam

PF00903. Glyoxalase. 2 hits.
[Graphical view]

ProDom

PD002334. Gly_diox. 2 hits.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR03211. catechol_2_3. 1 hit.

PROSITE

PS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

XYLE1_PSEPU

Accession

Primary (citable) accession number: P06622

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)