Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Carboxypeptidase G2

Gene

cpg2

Organism
Pseudomonas sp. (strain RS-16)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity.

Catalytic activityi

Release of C-terminal glutamate residues from a wide range of N-acylating moieties, including peptidyl, aminoacyl, benzoyl, benzyloxycarbonyl, folyl and pteroyl groups.

Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi112Zinc 21 Publication1
Active sitei114By similarity1
Metal bindingi141Zinc 11 Publication1
Metal bindingi141Zinc 21 Publication1
Active sitei175Proton acceptor1 Publication1
Metal bindingi176Zinc 11 Publication1
Metal bindingi200Zinc 21 Publication1
Metal bindingi385Zinc 11 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.11. 5085.
SABIO-RKP06621.

Protein family/group databases

MEROPSiM20.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase G2 (EC:3.4.17.11)
Short name:
CPDG2
Alternative name(s):
Folate hydrolase G2
Glutamate carboxypeptidase
Pteroylmonoglutamic acid hydrolase G2
INN: Glucarpidase
Gene namesi
Name:cpg2
OrganismiPseudomonas sp. (strain RS-16)
Taxonomic identifieri312 [NCBI]
Taxonomic lineageiBacteriaProteobacteria

Pathology & Biotechi

Pharmaceutical usei

Used clinically as a folate-depleting, antitumor agent.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000002680823 – 415Carboxypeptidase G2Add BLAST393

Proteomic databases

PRIDEiP06621.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1415
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 51Combined sources22
Helixi60 – 76Combined sources17
Beta strandi80 – 85Combined sources6
Beta strandi92 – 100Combined sources9
Beta strandi107 – 112Combined sources6
Helixi121 – 124Combined sources4
Beta strandi128 – 130Combined sources3
Beta strandi133 – 135Combined sources3
Turni137 – 142Combined sources6
Helixi143 – 158Combined sources16
Beta strandi164 – 173Combined sources10
Helixi175 – 177Combined sources3
Turni178 – 182Combined sources5
Helixi183 – 192Combined sources10
Beta strandi194 – 198Combined sources5
Beta strandi208 – 213Combined sources6
Beta strandi215 – 224Combined sources10
Helixi234 – 236Combined sources3
Helixi240 – 251Combined sources12
Helixi252 – 254Combined sources3
Turni257 – 260Combined sources4
Beta strandi261 – 270Combined sources10
Beta strandi279 – 291Combined sources13
Helixi292 – 306Combined sources15
Beta strandi315 – 322Combined sources8
Helixi330 – 346Combined sources17
Beta strandi352 – 355Combined sources4
Helixi363 – 366Combined sources4
Helixi367 – 369Combined sources3
Beta strandi380 – 382Combined sources3
Beta strandi386 – 388Combined sources3
Beta strandi391 – 393Combined sources3
Helixi394 – 396Combined sources3
Helixi397 – 412Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CG2X-ray2.50A/B/C/D23-415[»]
ProteinModelPortaliP06621.
SMRiP06621.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06621.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd03885. M20_CPDG2. 1 hit.
Gene3Di3.30.70.360. 1 hit.
InterProiIPR001261. ArgE/DapE_CS.
IPR017150. Pept_M20_glutamate_carboxypep.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF037238. Carboxypeptidase_G2. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPSIHRTAI AAVLATAFVA GTALAQKRDN VLFQAATDEQ PAVIKTLEKL
60 70 80 90 100
VNIETGTGDA EGIAAAGNFL EAELKNLGFT VTRSKSAGLV VGDNIVGKIK
110 120 130 140 150
GRGGKNLLLM SHMDTVYLKG ILAKAPFRVE GDKAYGPGIA DDKGGNAVIL
160 170 180 190 200
HTLKLLKEYG VRDYGTITVL FNTDEEKGSF GSRDLIQEEA KLADYVLSFE
210 220 230 240 250
PTSAGDEKLS LGTSGIAYVQ VNITGKASHA GAAPELGVNA LVEASDLVLR
260 270 280 290 300
TMNIDDKAKN LRFNWTIAKA GNVSNIIPAS ATLNADVRYA RNEDFDAAMK
310 320 330 340 350
TLEERAQQKK LPEADVKVIV TRGRPAFNAG EGGKKLVDKA VAYYKEAGGT
360 370 380 390 400
LGVEERTGGG TDAAYAALSG KPVIESLGLP GFGYHSDKAE YVDISAIPRR
410
LYMAARLIMD LGAGK
Length:415
Mass (Da):43,932
Last modified:January 1, 1988 - v1
Checksum:i8483A52202CE9C5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12599 Genomic DNA. Translation: AAA62842.1.
PIRiA24955. YXPSF2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12599 Genomic DNA. Translation: AAA62842.1.
PIRiA24955. YXPSF2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CG2X-ray2.50A/B/C/D23-415[»]
ProteinModelPortaliP06621.
SMRiP06621.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM20.001.

Proteomic databases

PRIDEiP06621.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.17.11. 5085.
SABIO-RKP06621.

Miscellaneous databases

EvolutionaryTraceiP06621.

Family and domain databases

CDDicd03885. M20_CPDG2. 1 hit.
Gene3Di3.30.70.360. 1 hit.
InterProiIPR001261. ArgE/DapE_CS.
IPR017150. Pept_M20_glutamate_carboxypep.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF037238. Carboxypeptidase_G2. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPG_PSES6
AccessioniPrimary (citable) accession number: P06621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Pharmaceutical

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.