Ice nucleation protein - Pseudomonas syringae pv. syringae

Preferred order of sections

Names and origin

Protein names

Recommended name:
Ice nucleation protein

Gene names

Name:

inaZ

Organism

Pseudomonas syringae pv. syringae

Taxonomic identifier

321 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	1200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ice nucleation proteins enable bacteria to nucleate crystallization in supercooled water.
Subcellular location	Cell outer membrane Ref.2.
Domain	Contains 122 imperfect repeats of a consensus octapeptide A-G-Y-G-S-T-L-T; further on a 16-residue and a regional 48-residue periodicity is superimposed.
Biotechnological use	Used by snowmaking industry to promote ice nucleation for artificial snow and economize water. Sold under the name Snomax by York. Ref.4
Miscellaneous	A structural model is suggested in which the ice nucleation protein displays a symmetry related to that of ice.
Sequence similarities	Belongs to the bacterial ice nucleation protein family.

Ontologies

Keywords
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Repeat
Molecular function	Ice nucleation
Technical term	3D-structure
Gene Ontology (GO)
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ice binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1200

1200

Ice nucleation protein

PRO_0000204024

Regions

Region

176 – 1151

976

Octapeptide periodicity

Secondary structure

1

.

1200

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P06620 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: E3BEAD4BEA67ABB0
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FASTA

1,200

118,588

        10         20         30         40         50         60 
MNLDKALVLR TCANNMADHC GLIWPASGTV ESRYWQSTRR HENGLVGLLW GAGTSAFLSV 

        70         80         90        100        110        120 
HADARWIVCE VAVADIISLE EPGMVKFPRA EVVHVGDRIS ASHFISARQA DPASTSTSTL 

       130        140        150        160        170        180 
TPMPTAIPTP MPAVASVTLP VAEQARHEVF DVASVSAAAA PVNTLPVTTP QNVQTATYGS 

       190        200        210        220        230        240 
TLSGDNHSRL IAGYGSNETA GNHSDLIAGY GSTGTAGSDS WLVAGYGSTQ TAGGDSALTA 

       250        260        270        280        290        300 
GYGSTQTARE GSNLTAGYGS TGTAGSDSSL IAGYGSTQTS GGDSSLTAGY GSTQTAQEGS 

       310        320        330        340        350        360 
NLTAGYGSTG TAGSDSSLIA GYGSTQTSGG DSSLTAGYGS TQTAQEGSNL TAGYGSTGTA 

       370        380        390        400        410        420 
GVDSSLIAGY GSTQTSGSDS ALTAGYGSTQ TAQEGSNLTA GYGSTGTAGS DSSLIAGYGS 

       430        440        450        460        470        480 
TQTSGSDSSL TAGYGSTQTA QEGSILTAGY GSTGTAGVDS SLIAGYGSTQ TSGSDSALTA 

       490        500        510        520        530        540 
GYGSTQTAQE GSNLTAGYGS TGTAGADSSL IAGYGSTQTS GSESSLTAGY GSTQTAREGS 

       550        560        570        580        590        600 
TLTAGYGSTG TAGADSSLIA GYGSTQTSGS ESSLTAGYGS TQTAQQGSVL TSGYGSTQTA 

       610        620        630        640        650        660 
GAASNLTTGY GSTGTAGHES FIIAGYGSTQ TAGHKSILTA GYGSTQTARD GSDLIAGYGS 

       670        680        690        700        710        720 
TGTAGSGSSL IAGYGSTQTA SYRSMLTAGY GSTQTAREHS DLVTGYGSTS TAGSNSSLIA 

       730        740        750        760        770        780 
GYGSTQTAGF KSILTAGYGS TQTAQERTSL VAGYGSTSTA GYSSSLIAGY GSTQTAGYES 

       790        800        810        820        830        840 
TLTAGYGSTQ TAQENSSLTT GYGSTSTAGY SSSLIAGYGS TQTAGYESTL TAGYGSTQTA 

       850        860        870        880        890        900 
QERSDLVTGY GSTSTAGYAS SLIAGYGSTQ TAGYESTLTA GYGSTQTAQE NSSLTTGYGS 

       910        920        930        940        950        960 
TSTAGFASSL ISGYGSTQTA GYKSTLTAGY GSTQTAEYGS SLTAGYGSTA TAGQDSSLIA 

       970        980        990       1000       1010       1020 
GYGSSLTSGI RSFLTAGYGS TLIAGLRSVL IAGYGSSLTS GVRSTLTAGY GSNQIASYGS 

      1030       1040       1050       1060       1070       1080 
SLIAGHESIQ VAGNKSMLIA GKGSSQTAGF RSTLIAGAGS VQLAGDRSRL IAGADSNQTA 

      1090       1100       1110       1120       1130       1140 
GDRSKLLAGN NSYLTAGDRS KLTGGHDCTL MAGDQSRLTA GKNSVLTAGA RSKLIGSEGS 

      1150       1160       1170       1180       1190       1200 
TLSAGEDSIL IFRLWDGKRY RQLVARTGEN GVEADIPYYV NEDDDIVDKP DEDDDWIEVK

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References

[1]	"Physical and functional repetition in a bacterial ice nucleation gene." Green R.L., Warren G.J. Nature 317:645-648(1985) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S203.
[2]	"Localization of ice nucleation activity and the iceC gene product in Pseudomonas syringae and Escherichia coli." Lindow S.E., Lahue E., Govindarajan A.G., Panopoulos N.J., Gies D. Mol. Plant Microbe Interact. 2:262-272(1989) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[3]	"A model of the three-dimensional structure of ice nucleation proteins." Kajava A.V., Lindow S.E. J. Mol. Biol. 232:709-717(1993) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 490-535.
[4]	"Principles and biotechnological applications of bacterial ice nucleation." Margaritis A., Bassi A.S. Crit. Rev. Biotechnol. 11:277-295(1991) [PubMed] [Europe PMC] [Abstract] Cited for: BIOTECHNOLOGICAL RELEVANCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03035 Genomic DNA. Translation: CAA26837.1.

PIR

SNPSO. A24405.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1INA	model	-	A	490-535	[»]
1INB	model	-	A	490-535	[»]

ProteinModelPortal

P06620.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000258. Ice_nucleatn.
[Graphical view]

Pfam

PF00818. Ice_nucleation. 28 hits.
[Graphical view]

PRINTS

PR00327. ICENUCLEATN.

PROSITE

PS00314. ICE_NUCLEATION. 40 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ICEN_PSESY

Accession

Primary (citable) accession number: P06620

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families