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P06620 (ICEN_PSESY) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ice nucleation protein
Gene names
Name:inaZ
OrganismPseudomonas syringae pv. syringae
Taxonomic identifier321 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae

Protein attributes

Sequence length1200 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ice nucleation proteins enable bacteria to nucleate crystallization in supercooled water.

Subcellular location

Cell outer membrane Ref.2.

Domain

Contains 122 imperfect repeats of a consensus octapeptide A-G-Y-G-S-T-L-T; further on a 16-residue and a regional 48-residue periodicity is superimposed.

Biotechnological use

Used by snowmaking industry to promote ice nucleation for artificial snow and economize water. Sold under the name Snomax by York. Ref.4

Miscellaneous

A structural model is suggested in which the ice nucleation protein displays a symmetry related to that of ice.

Sequence similarities

Belongs to the bacterial ice nucleation protein family.

Ontologies

Keywords
   Cellular componentCell outer membrane
Membrane
   DomainRepeat
   Molecular functionIce nucleation
   Technical term3D-structure
Gene Ontology (GO)
   Cellular_componentcell outer membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionice binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12001200Ice nucleation protein
PRO_0000204024

Regions

Region176 – 1151976Octapeptide periodicity

Secondary structure

................. 1200
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06620 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: E3BEAD4BEA67ABB0

FASTA1,200118,588
        10         20         30         40         50         60 
MNLDKALVLR TCANNMADHC GLIWPASGTV ESRYWQSTRR HENGLVGLLW GAGTSAFLSV 

        70         80         90        100        110        120 
HADARWIVCE VAVADIISLE EPGMVKFPRA EVVHVGDRIS ASHFISARQA DPASTSTSTL 

       130        140        150        160        170        180 
TPMPTAIPTP MPAVASVTLP VAEQARHEVF DVASVSAAAA PVNTLPVTTP QNVQTATYGS 

       190        200        210        220        230        240 
TLSGDNHSRL IAGYGSNETA GNHSDLIAGY GSTGTAGSDS WLVAGYGSTQ TAGGDSALTA 

       250        260        270        280        290        300 
GYGSTQTARE GSNLTAGYGS TGTAGSDSSL IAGYGSTQTS GGDSSLTAGY GSTQTAQEGS 

       310        320        330        340        350        360 
NLTAGYGSTG TAGSDSSLIA GYGSTQTSGG DSSLTAGYGS TQTAQEGSNL TAGYGSTGTA 

       370        380        390        400        410        420 
GVDSSLIAGY GSTQTSGSDS ALTAGYGSTQ TAQEGSNLTA GYGSTGTAGS DSSLIAGYGS 

       430        440        450        460        470        480 
TQTSGSDSSL TAGYGSTQTA QEGSILTAGY GSTGTAGVDS SLIAGYGSTQ TSGSDSALTA 

       490        500        510        520        530        540 
GYGSTQTAQE GSNLTAGYGS TGTAGADSSL IAGYGSTQTS GSESSLTAGY GSTQTAREGS 

       550        560        570        580        590        600 
TLTAGYGSTG TAGADSSLIA GYGSTQTSGS ESSLTAGYGS TQTAQQGSVL TSGYGSTQTA 

       610        620        630        640        650        660 
GAASNLTTGY GSTGTAGHES FIIAGYGSTQ TAGHKSILTA GYGSTQTARD GSDLIAGYGS 

       670        680        690        700        710        720 
TGTAGSGSSL IAGYGSTQTA SYRSMLTAGY GSTQTAREHS DLVTGYGSTS TAGSNSSLIA 

       730        740        750        760        770        780 
GYGSTQTAGF KSILTAGYGS TQTAQERTSL VAGYGSTSTA GYSSSLIAGY GSTQTAGYES 

       790        800        810        820        830        840 
TLTAGYGSTQ TAQENSSLTT GYGSTSTAGY SSSLIAGYGS TQTAGYESTL TAGYGSTQTA 

       850        860        870        880        890        900 
QERSDLVTGY GSTSTAGYAS SLIAGYGSTQ TAGYESTLTA GYGSTQTAQE NSSLTTGYGS 

       910        920        930        940        950        960 
TSTAGFASSL ISGYGSTQTA GYKSTLTAGY GSTQTAEYGS SLTAGYGSTA TAGQDSSLIA 

       970        980        990       1000       1010       1020 
GYGSSLTSGI RSFLTAGYGS TLIAGLRSVL IAGYGSSLTS GVRSTLTAGY GSNQIASYGS 

      1030       1040       1050       1060       1070       1080 
SLIAGHESIQ VAGNKSMLIA GKGSSQTAGF RSTLIAGAGS VQLAGDRSRL IAGADSNQTA 

      1090       1100       1110       1120       1130       1140 
GDRSKLLAGN NSYLTAGDRS KLTGGHDCTL MAGDQSRLTA GKNSVLTAGA RSKLIGSEGS 

      1150       1160       1170       1180       1190       1200 
TLSAGEDSIL IFRLWDGKRY RQLVARTGEN GVEADIPYYV NEDDDIVDKP DEDDDWIEVK 

« Hide

References

[1]"Physical and functional repetition in a bacterial ice nucleation gene."
Green R.L., Warren G.J.
Nature 317:645-648(1985)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S203.
[2]"Localization of ice nucleation activity and the iceC gene product in Pseudomonas syringae and Escherichia coli."
Lindow S.E., Lahue E., Govindarajan A.G., Panopoulos N.J., Gies D.
Mol. Plant Microbe Interact. 2:262-272(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[3]"A model of the three-dimensional structure of ice nucleation proteins."
Kajava A.V., Lindow S.E.
J. Mol. Biol. 232:709-717(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 490-535.
[4]"Principles and biotechnological applications of bacterial ice nucleation."
Margaritis A., Bassi A.S.
Crit. Rev. Biotechnol. 11:277-295(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTECHNOLOGICAL RELEVANCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03035 Genomic DNA. Translation: CAA26837.1.
PIRSNPSO. A24405.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1INAmodel-A490-535[»]
1INBmodel-A490-535[»]
ProteinModelPortalP06620.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB9.B.133.1.1. the ice nucleation protein secretion system (inp-ss) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000258. Ice_nucleatn.
[Graphical view]
PfamPF00818. Ice_nucleation. 28 hits.
[Graphical view]
PRINTSPR00327. ICENUCLEATN.
PROSITEPS00314. ICE_NUCLEATION. 40 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameICEN_PSESY
AccessionPrimary (citable) accession number: P06620
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 13, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references