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Protein

HTH-type transcriptional regulator CysB

Gene

cysB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a positive regulator of gene expression for the cysteine regulon, a system of 10 or more loci involved in the biosynthesis of L-cysteine from inorganic sulfate. The inducer for CysB is N-acetylserine. CysB inhibits its own transcription.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi19 – 38H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator CysB
Alternative name(s):
Cys regulon transcriptional activator
Gene namesi
Name:cysB
Ordered Locus Names:STM1713
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001056161 – 324HTH-type transcriptional regulator CysBAdd BLAST324

Proteomic databases

PaxDbiP06614.
PRIDEiP06614.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi99287.STM1713.

Structurei

Secondary structure

1324
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi93 – 99Combined sources7
Helixi101 – 106Combined sources6
Helixi109 – 118Combined sources10
Beta strandi122 – 128Combined sources7
Helixi131 – 139Combined sources9
Beta strandi144 – 149Combined sources6
Helixi152 – 154Combined sources3
Beta strandi158 – 166Combined sources9
Beta strandi168 – 172Combined sources5
Helixi177 – 180Combined sources4
Helixi186 – 189Combined sources4
Beta strandi192 – 197Combined sources6
Beta strandi201 – 203Combined sources3
Helixi204 – 213Combined sources10
Beta strandi219 – 225Combined sources7
Helixi227 – 234Combined sources8
Turni235 – 237Combined sources3
Beta strandi240 – 244Combined sources5
Helixi245 – 247Combined sources3
Turni250 – 252Combined sources3
Beta strandi257 – 260Combined sources4
Turni262 – 264Combined sources3
Beta strandi268 – 274Combined sources7
Beta strandi276 – 278Combined sources3
Helixi282 – 291Combined sources10
Helixi297 – 306Combined sources10
Helixi309 – 315Combined sources7
Turni316 – 318Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GWOX-ray2.39A/B1-324[»]
4GXAX-ray2.81A/B86-324[»]
4LONX-ray2.20B86-324[»]
4LP2X-ray2.30B86-324[»]
4LQ2X-ray2.69A86-324[»]
4LQ5X-ray2.80A86-324[»]
4M4GX-ray2.70A86-324[»]
ProteinModelPortaliP06614.
SMRiP06614.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 59HTH lysR-typePROSITE-ProRule annotationAdd BLAST59

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107R11. Bacteria.
ENOG410XP61. LUCA.
HOGENOMiHOG000260068.
KOiK13634.
OMAiDEAFSHR.
PhylomeDBiP06614.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQQLRYIV EVVNHNLNVS STAEGLYTSQ PGISKQVRML EDELGIQIFA
60 70 80 90 100
RSGKHLTQVT PAGQEIIRIA REVLSKVDAI KSVAGEHTWP DKGSLYIATT
110 120 130 140 150
HTQARYALPG VIKGFIERYP RVSLHMHQGS PTQIAEAVSK GNADFAIATE
160 170 180 190 200
ALHLYDDLVM LPCYHWNRSI VVTPDHPLAA TSSVTIEALA QYPLVTYTFG
210 220 230 240 250
FTGRSELDTA FNRAGLTPRI VFTATDADVI KTYVRLGLGV GVIASMAVDP
260 270 280 290 300
LADPDLVRID AHDIFSHSTT KIGFRRSTFL RSYMYDFIQR FAPHLTRDVV
310 320
DTAVALRSNE EIEAMFQDIK LPEK
Length:324
Mass (Da):36,013
Last modified:January 1, 1988 - v1
Checksum:iCCECF585A6851D34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15040 Genomic DNA. Translation: AAA27045.1.
AE006468 Genomic DNA. Translation: AAL20631.1.
PIRiB26695. RGEBCB.
RefSeqiNP_460672.1. NC_003197.1.
WP_000776233.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20631; AAL20631; STM1713.
GeneIDi1253232.
KEGGistm:STM1713.
PATRICi32381941. VBISalEnt20916_1810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15040 Genomic DNA. Translation: AAA27045.1.
AE006468 Genomic DNA. Translation: AAL20631.1.
PIRiB26695. RGEBCB.
RefSeqiNP_460672.1. NC_003197.1.
WP_000776233.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GWOX-ray2.39A/B1-324[»]
4GXAX-ray2.81A/B86-324[»]
4LONX-ray2.20B86-324[»]
4LP2X-ray2.30B86-324[»]
4LQ2X-ray2.69A86-324[»]
4LQ5X-ray2.80A86-324[»]
4M4GX-ray2.70A86-324[»]
ProteinModelPortaliP06614.
SMRiP06614.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1713.

Proteomic databases

PaxDbiP06614.
PRIDEiP06614.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20631; AAL20631; STM1713.
GeneIDi1253232.
KEGGistm:STM1713.
PATRICi32381941. VBISalEnt20916_1810.

Phylogenomic databases

eggNOGiENOG4107R11. Bacteria.
ENOG410XP61. LUCA.
HOGENOMiHOG000260068.
KOiK13634.
OMAiDEAFSHR.
PhylomeDBiP06614.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSB_SALTY
AccessioniPrimary (citable) accession number: P06614
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.