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Protein

HTH-type transcriptional regulator CysB

Gene

cysB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a positive regulator of gene expression for the cysteine regulon, a system of 10 or more loci involved in the biosynthesis of L-cysteine from inorganic sulfate. The inducer for CysB is N-acetylserine. CysB inhibits its own transcription.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi19 – 3820H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1722-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator CysB
Alternative name(s):
Cys regulon transcriptional activator
Gene namesi
Name:cysB
Ordered Locus Names:STM1713
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324HTH-type transcriptional regulator CysBPRO_0000105616Add
BLAST

Proteomic databases

PaxDbiP06614.
PRIDEiP06614.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi99287.STM1713.

Structurei

Secondary structure

1
324
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi93 – 997Combined sources
Helixi101 – 1066Combined sources
Helixi109 – 11810Combined sources
Beta strandi122 – 1287Combined sources
Helixi131 – 1399Combined sources
Beta strandi144 – 1496Combined sources
Helixi152 – 1543Combined sources
Beta strandi158 – 1669Combined sources
Beta strandi168 – 1725Combined sources
Helixi177 – 1804Combined sources
Helixi186 – 1894Combined sources
Beta strandi192 – 1976Combined sources
Beta strandi201 – 2033Combined sources
Helixi204 – 21310Combined sources
Beta strandi219 – 2257Combined sources
Helixi227 – 2348Combined sources
Turni235 – 2373Combined sources
Beta strandi240 – 2445Combined sources
Helixi245 – 2473Combined sources
Turni250 – 2523Combined sources
Beta strandi257 – 2604Combined sources
Turni262 – 2643Combined sources
Beta strandi268 – 2747Combined sources
Beta strandi276 – 2783Combined sources
Helixi282 – 29110Combined sources
Helixi297 – 30610Combined sources
Helixi309 – 3157Combined sources
Turni316 – 3183Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GWOX-ray2.39A/B1-324[»]
4GXAX-ray2.81A/B86-324[»]
4LONX-ray2.20B86-324[»]
4LP2X-ray2.30B86-324[»]
4LQ2X-ray2.69A86-324[»]
4LQ5X-ray2.80A86-324[»]
4M4GX-ray2.70A86-324[»]
ProteinModelPortaliP06614.
SMRiP06614. Positions 88-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5959HTH lysR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107R11. Bacteria.
ENOG410XP61. LUCA.
HOGENOMiHOG000260068.
KOiK13634.
OMAiDEAFSHR.
PhylomeDBiP06614.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQQLRYIV EVVNHNLNVS STAEGLYTSQ PGISKQVRML EDELGIQIFA
60 70 80 90 100
RSGKHLTQVT PAGQEIIRIA REVLSKVDAI KSVAGEHTWP DKGSLYIATT
110 120 130 140 150
HTQARYALPG VIKGFIERYP RVSLHMHQGS PTQIAEAVSK GNADFAIATE
160 170 180 190 200
ALHLYDDLVM LPCYHWNRSI VVTPDHPLAA TSSVTIEALA QYPLVTYTFG
210 220 230 240 250
FTGRSELDTA FNRAGLTPRI VFTATDADVI KTYVRLGLGV GVIASMAVDP
260 270 280 290 300
LADPDLVRID AHDIFSHSTT KIGFRRSTFL RSYMYDFIQR FAPHLTRDVV
310 320
DTAVALRSNE EIEAMFQDIK LPEK
Length:324
Mass (Da):36,013
Last modified:January 1, 1988 - v1
Checksum:iCCECF585A6851D34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15040 Genomic DNA. Translation: AAA27045.1.
AE006468 Genomic DNA. Translation: AAL20631.1.
PIRiB26695. RGEBCB.
RefSeqiNP_460672.1. NC_003197.1.
WP_000776233.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20631; AAL20631; STM1713.
GeneIDi1253232.
KEGGistm:STM1713.
PATRICi32381941. VBISalEnt20916_1810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15040 Genomic DNA. Translation: AAA27045.1.
AE006468 Genomic DNA. Translation: AAL20631.1.
PIRiB26695. RGEBCB.
RefSeqiNP_460672.1. NC_003197.1.
WP_000776233.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GWOX-ray2.39A/B1-324[»]
4GXAX-ray2.81A/B86-324[»]
4LONX-ray2.20B86-324[»]
4LP2X-ray2.30B86-324[»]
4LQ2X-ray2.69A86-324[»]
4LQ5X-ray2.80A86-324[»]
4M4GX-ray2.70A86-324[»]
ProteinModelPortaliP06614.
SMRiP06614. Positions 88-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1713.

Proteomic databases

PaxDbiP06614.
PRIDEiP06614.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20631; AAL20631; STM1713.
GeneIDi1253232.
KEGGistm:STM1713.
PATRICi32381941. VBISalEnt20916_1810.

Phylogenomic databases

eggNOGiENOG4107R11. Bacteria.
ENOG410XP61. LUCA.
HOGENOMiHOG000260068.
KOiK13634.
OMAiDEAFSHR.
PhylomeDBiP06614.

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1722-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSB_SALTY
AccessioniPrimary (citable) accession number: P06614
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.