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Protein

Vitamin B12 import ATP-binding protein BtuD

Gene

btuD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Responsible for energy coupling to the transport system.UniRule annotation

Catalytic activityi

ATP + H2O + vitamin B12(Out) = ADP + phosphate + vitamin B12(In).UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 40ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

  • cobalamin transport Source: EcoCyc

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:BTUD-MONOMER
MetaCyc:BTUD-MONOMER
BRENDAi3.6.3.33 2026

Protein family/group databases

TCDBi3.A.1.13.1 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12 import ATP-binding protein BtuDUniRule annotation (EC:3.6.3.33UniRule annotation)
Alternative name(s):
Vitamin B12-transporting ATPaseUniRule annotation
Gene namesi
Name:btuDUniRule annotation
Ordered Locus Names:b1709, JW1699
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10128 btuD

Subcellular locationi

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: EcoCyc
  • extrinsic component of membrane Source: EcoliWiki

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000919501 – 249Vitamin B12 import ATP-binding protein BtuDAdd BLAST249

Proteomic databases

PaxDbiP06611
PRIDEiP06611

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (BtuD), two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
btuCP0660912EBI-1033420,EBI-1033427

Protein-protein interaction databases

BioGridi4260301, 185 interactors
DIPiDIP-9234N
IntActiP06611, 9 interactors
MINTiP06611
STRINGi316385.ECDH10B_1845

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 12Combined sources10
Turni13 – 15Combined sources3
Beta strandi16 – 24Combined sources9
Beta strandi28 – 32Combined sources5
Helixi39 – 46Combined sources8
Beta strandi53 – 58Combined sources6
Helixi63 – 65Combined sources3
Helixi68 – 74Combined sources7
Beta strandi75 – 78Combined sources4
Helixi90 – 95Combined sources6
Helixi104 – 113Combined sources10
Helixi117 – 119Combined sources3
Beta strandi120 – 123Combined sources4
Helixi124 – 126Combined sources3
Helixi129 – 144Combined sources16
Turni146 – 148Combined sources3
Beta strandi154 – 159Combined sources6
Turni160 – 163Combined sources4
Helixi166 – 181Combined sources16
Beta strandi185 – 189Combined sources5
Helixi193 – 199Combined sources7
Beta strandi201 – 207Combined sources7
Beta strandi210 – 216Combined sources7
Helixi217 – 220Combined sources4
Helixi223 – 230Combined sources8
Beta strandi234 – 239Combined sources6
Beta strandi242 – 247Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L7VX-ray3.20C/D1-249[»]
2QI9X-ray2.60C/D1-249[»]
4DBLX-ray3.49C/D/H/I1-249[»]
4FI3X-ray3.47C/D1-249[»]
4R9UX-ray2.78C/D1-249[»]
ProteinModelPortaliP06611
SMRiP06611
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06611

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 233ABC transporterUniRule annotationAdd BLAST233

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Vitamin B12 importer (TC 3.A.1.13.1) family. [View classification]UniRule annotation

Phylogenomic databases

eggNOGiENOG41080WI Bacteria
COG4138 LUCA
InParanoidiP06611
KOiK06074
OMAiHHADRVW
PhylomeDBiP06611

Family and domain databases

HAMAPiMF_01005 BtuD, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR023693 ABC_transptr_BtuD
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit

Sequencei

Sequence statusi: Complete.

P06611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIVMQLQDV AESTRLGPLS GEVRAGEILH LVGPNGAGKS TLLARMAGMT
60 70 80 90 100
SGKGSIQFAG QPLEAWSATK LALHRAYLSQ QQTPPFATPV WHYLTLHQHD
110 120 130 140 150
KTRTELLNDV AGALALDDKL GRSTNQLSGG EWQRVRLAAV VLQITPQANP
160 170 180 190 200
AGQLLLLDEP MNSLDVAQQS ALDKILSALC QQGLAIVMSS HDLNHTLRHA
210 220 230 240
HRAWLLKGGK MLASGRREEV LTPPNLAQAY GMNFRRLDIE GHRMLISTI
Length:249
Mass (Da):27,081
Last modified:January 1, 1988 - v1
Checksum:i760004A5C0134245
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14031 Genomic DNA Translation: AAA23528.1
U00096 Genomic DNA Translation: AAC74779.1
AP009048 Genomic DNA Translation: BAA15477.1
PIRiC24498 QRECBD
RefSeqiNP_416224.1, NC_000913.3
WP_000029474.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74779; AAC74779; b1709
BAA15477; BAA15477; BAA15477
GeneIDi945751
KEGGiecj:JW1699
eco:b1709
PATRICifig|1411691.4.peg.548

Similar proteinsi

Entry informationi

Entry nameiBTUD_ECOLI
AccessioniPrimary (citable) accession number: P06611
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: March 28, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. UniProtKB entry view manual
    User manual for the UniProtKB entry view

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