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Protein

Vitamin B12 import ATP-binding protein BtuD

Gene

btuD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Responsible for energy coupling to the transport system.UniRule annotation

Catalytic activityi

ATP + H2O + vitamin B12(Out) = ADP + phosphate + vitamin B12(In).UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 40ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • cobalamin-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  • cobalamin transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:BTUD-MONOMER.
ECOL316407:JW1699-MONOMER.
MetaCyc:BTUD-MONOMER.
BRENDAi3.6.3.33. 2026.

Protein family/group databases

TCDBi3.A.1.13.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12 import ATP-binding protein BtuDUniRule annotation (EC:3.6.3.33UniRule annotation)
Alternative name(s):
Vitamin B12-transporting ATPaseUniRule annotation
Gene namesi
Name:btuDUniRule annotation
Ordered Locus Names:b1709, JW1699
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10128. btuD.

Subcellular locationi

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: EcoCyc
  • cytosol Source: EcoCyc
  • extrinsic component of cytoplasmic side of plasma membrane Source: EcoCyc
  • extrinsic component of membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000919501 – 249Vitamin B12 import ATP-binding protein BtuDAdd BLAST249

Proteomic databases

PaxDbiP06611.
PRIDEiP06611.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (BtuD), two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
btuCP066093EBI-1033420,EBI-1033427

Protein-protein interaction databases

BioGridi4260301. 184 interactors.
DIPiDIP-9234N.
IntActiP06611. 9 interactors.
MINTiMINT-6803741.
STRINGi511145.b1709.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 12Combined sources10
Turni13 – 15Combined sources3
Beta strandi16 – 24Combined sources9
Beta strandi28 – 32Combined sources5
Helixi39 – 46Combined sources8
Beta strandi53 – 58Combined sources6
Helixi63 – 65Combined sources3
Helixi68 – 74Combined sources7
Beta strandi75 – 78Combined sources4
Helixi90 – 95Combined sources6
Helixi104 – 113Combined sources10
Helixi117 – 119Combined sources3
Beta strandi120 – 123Combined sources4
Helixi124 – 126Combined sources3
Helixi129 – 144Combined sources16
Turni146 – 148Combined sources3
Beta strandi154 – 159Combined sources6
Turni160 – 163Combined sources4
Helixi166 – 181Combined sources16
Beta strandi185 – 189Combined sources5
Helixi193 – 199Combined sources7
Beta strandi201 – 207Combined sources7
Beta strandi210 – 216Combined sources7
Helixi217 – 220Combined sources4
Helixi223 – 230Combined sources8
Beta strandi234 – 239Combined sources6
Beta strandi242 – 247Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L7VX-ray3.20C/D1-249[»]
2QI9X-ray2.60C/D1-249[»]
4DBLX-ray3.49C/D/H/I1-249[»]
4FI3X-ray3.47C/D1-249[»]
4R9UX-ray2.78C/D1-249[»]
ProteinModelPortaliP06611.
SMRiP06611.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06611.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 233ABC transporterUniRule annotationAdd BLAST233

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Vitamin B12 importer (TC 3.A.1.13.1) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG41080WI. Bacteria.
COG4138. LUCA.
InParanoidiP06611.
KOiK06074.
OMAiHLAYYAR.
PhylomeDBiP06611.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01005. BtuD. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR023693. ABC_transptr_BtuD.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIVMQLQDV AESTRLGPLS GEVRAGEILH LVGPNGAGKS TLLARMAGMT
60 70 80 90 100
SGKGSIQFAG QPLEAWSATK LALHRAYLSQ QQTPPFATPV WHYLTLHQHD
110 120 130 140 150
KTRTELLNDV AGALALDDKL GRSTNQLSGG EWQRVRLAAV VLQITPQANP
160 170 180 190 200
AGQLLLLDEP MNSLDVAQQS ALDKILSALC QQGLAIVMSS HDLNHTLRHA
210 220 230 240
HRAWLLKGGK MLASGRREEV LTPPNLAQAY GMNFRRLDIE GHRMLISTI
Length:249
Mass (Da):27,081
Last modified:January 1, 1988 - v1
Checksum:i760004A5C0134245
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14031 Genomic DNA. Translation: AAA23528.1.
U00096 Genomic DNA. Translation: AAC74779.1.
AP009048 Genomic DNA. Translation: BAA15477.1.
PIRiC24498. QRECBD.
RefSeqiNP_416224.1. NC_000913.3.
WP_000029474.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74779; AAC74779; b1709.
BAA15477; BAA15477; BAA15477.
GeneIDi945751.
KEGGiecj:JW1699.
eco:b1709.
PATRICi32118724. VBIEscCol129921_1780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14031 Genomic DNA. Translation: AAA23528.1.
U00096 Genomic DNA. Translation: AAC74779.1.
AP009048 Genomic DNA. Translation: BAA15477.1.
PIRiC24498. QRECBD.
RefSeqiNP_416224.1. NC_000913.3.
WP_000029474.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L7VX-ray3.20C/D1-249[»]
2QI9X-ray2.60C/D1-249[»]
4DBLX-ray3.49C/D/H/I1-249[»]
4FI3X-ray3.47C/D1-249[»]
4R9UX-ray2.78C/D1-249[»]
ProteinModelPortaliP06611.
SMRiP06611.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260301. 184 interactors.
DIPiDIP-9234N.
IntActiP06611. 9 interactors.
MINTiMINT-6803741.
STRINGi511145.b1709.

Protein family/group databases

TCDBi3.A.1.13.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP06611.
PRIDEiP06611.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74779; AAC74779; b1709.
BAA15477; BAA15477; BAA15477.
GeneIDi945751.
KEGGiecj:JW1699.
eco:b1709.
PATRICi32118724. VBIEscCol129921_1780.

Organism-specific databases

EchoBASEiEB0126.
EcoGeneiEG10128. btuD.

Phylogenomic databases

eggNOGiENOG41080WI. Bacteria.
COG4138. LUCA.
InParanoidiP06611.
KOiK06074.
OMAiHLAYYAR.
PhylomeDBiP06611.

Enzyme and pathway databases

BioCyciEcoCyc:BTUD-MONOMER.
ECOL316407:JW1699-MONOMER.
MetaCyc:BTUD-MONOMER.
BRENDAi3.6.3.33. 2026.

Miscellaneous databases

EvolutionaryTraceiP06611.
PROiP06611.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01005. BtuD. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR023693. ABC_transptr_BtuD.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBTUD_ECOLI
AccessioniPrimary (citable) accession number: P06611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.