P06610 (BTUE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vitamin B12 transport periplasmic protein BtuE
EC=1.-.-.-

Gene names

Name:	btuE
Ordered Locus Names:	b1710, JW1700

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Not essential for B12 transport; however, it is an auxiliary component of the transport system.
Subcellular location	Periplasm Probable.
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Biological process	Cobalt transport Ion transport Transport
Cellular component	Periplasm
Ligand	Cobalt
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cobalt ion transport Inferred from electronic annotation. Source: UniProtKB-KW response to hydroperoxide Inferred from expression pattern PubMed 20621065. Source: EcoCyc
Cellular_component	periplasmic space Inferred from direct assay PubMed 3528128. Source: EcoliWiki
Molecular_function	glutathione peroxidase activity Inferred from direct assay PubMed 20621065. Source: EcoCyc thioredoxin peroxidase activity Inferred from direct assay PubMed 20621065. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	Vitamin B12 transport periplasmic protein BtuE		PRO_0000066664

Sequences

	Sequence		Length	Mass (Da)
	P06610 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: C8DB671963A7F235	FASTA	183	20,470
	10 20 30 40 50 60 MQDSILTTVV KDIDGEVTTL EKFAGNVLLI VNVASKCGLT PQYEQLENIQ KAWVDRGFMV 70 80 90 100 110 120 LGFPCNQFLE QEPGSDEEIK TYCTTTWGVT FPMFSKIEVN GEGRHPLYQK LIAAAPTAVA 130 140 150 160 170 180 PEESGFYARM VSKGRAPLYP DDILWNFEKF LVGRDGKVIQ RFSPDMTPED PIVMESIKLA LAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the btuCED genes involved in vitamin B12 transport in Escherichia coli and homology with components of periplasmic-binding-protein-dependent transport systems." Friedrich M.J., Deveaux L.C., Kadner R.J. J. Bacteriol. 167:928-934(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14031 Genomic DNA. Translation: AAA23527.1. U00096 Genomic DNA. Translation: AAC74780.1. AP009048 Genomic DNA. Translation: BAA15478.1.
PIR	QRECBE. F64929.
RefSeq	NP_416225.1. NC_000913.2. YP_489972.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P06610.
SMR	P06610. Positions 15-163.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06610. 17 interactions.
STRING	511145.b1710.
Protein family/group databases
PeroxiBase	3987. EcoGPx01.
Proteomic databases
PaxDb	P06610.
PRIDE	P06610.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74780; AAC74780; b1710. BAA15478; BAA15478; BAA15478.
GeneID	12931292. 945915.
KEGG	ecj:Y75_p1685. eco:b1710.
PATRIC	32118726. VBIEscCol129921_1781.
Organism-specific databases
EchoBASE	EB0127.
EcoGene	EG10129. btuE.
Phylogenomic databases
eggNOG	COG0386.
HOGENOM	HOG000277053.
KO	K00432.
OMA	EANGTWK.
ProtClustDB	PRK10606.
Enzyme and pathway databases
BioCyc	EcoCyc:BTUE-MONOMER. ECOL316407:JW1700-MONOMER. MetaCyc:BTUE-MONOMER.
Gene expression databases
Genevestigator	P06610.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR000889. Glutathione_peroxidase. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11592. PTHR11592. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BTUE_ECOLI

Accession

Primary (citable) accession number: P06610

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program