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Protein

Vitamin B12 import system permease protein BtuC

Gene

btuC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Involved in the translocation of the substrate across the membrane.

GO - Molecular functioni

  • cobalamin transporter activity Source: EcoliWiki
  • cobalamin-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  • cobalamin transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:BTUC-MONOMER.
ECOL316407:JW1701-MONOMER.
MetaCyc:BTUC-MONOMER.
BRENDAi3.6.3.33. 2026.

Protein family/group databases

TCDBi3.A.1.13.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12 import system permease protein BtuC
Gene namesi
Name:btuC
Ordered Locus Names:b1711, JW1701
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10127. btuC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10Cytoplasmic1 Publication10
Transmembranei11 – 35Helical; Name=1Add BLAST25
Topological domaini36 – 56Periplasmic1 PublicationAdd BLAST21
Transmembranei57 – 81Helical; Name=2Add BLAST25
Topological domaini82 – 92Cytoplasmic1 PublicationAdd BLAST11
Transmembranei93 – 107Helical; Name=3Add BLAST15
Topological domaini108 – 113Periplasmic1 Publication6
Transmembranei114 – 138Helical; Name=4Add BLAST25
Topological domaini139 – 141Cytoplasmic1 Publication3
Transmembranei142 – 166Helical; Name=5Add BLAST25
Topological domaini167 – 190Periplasmic1 PublicationAdd BLAST24
Transmembranei191 – 206Helical; Name=6Add BLAST16
Topological domaini207 – 228Cytoplasmic1 PublicationAdd BLAST22
Transmembranei229 – 249Helical; Name=7Add BLAST21
Topological domaini250 – 257Periplasmic1 Publication8
Transmembranei258 – 267Helical; Name=810
Topological domaini268 – 274Cytoplasmic1 Publication7
Transmembranei275 – 296Helical; Name=9Add BLAST22
Topological domaini297 – 304Periplasmic1 Publication8
Transmembranei305 – 324Helical; Name=10Add BLAST20
Topological domaini325 – 326Cytoplasmic1 Publication2

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000599681 – 326Vitamin B12 import system permease protein BtuCAdd BLAST326

Proteomic databases

PaxDbiP06609.
PRIDEiP06609.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (BtuD), two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
btuDP066113EBI-1033427,EBI-1033420

Protein-protein interaction databases

BioGridi4263152. 131 interactors.
DIPiDIP-9233N.
IntActiP06609. 2 interactors.
MINTiMINT-6803761.
STRINGi511145.b1711.

Structurei

Secondary structure

1326
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 31Combined sources29
Beta strandi34 – 36Combined sources3
Helixi40 – 42Combined sources3
Beta strandi43 – 45Combined sources3
Helixi48 – 54Combined sources7
Helixi56 – 80Combined sources25
Helixi88 – 91Combined sources4
Helixi93 – 108Combined sources16
Helixi114 – 136Combined sources23
Turni137 – 139Combined sources3
Helixi142 – 165Combined sources24
Helixi171 – 179Combined sources9
Helixi188 – 190Combined sources3
Helixi191 – 194Combined sources4
Turni195 – 197Combined sources3
Helixi198 – 204Combined sources7
Helixi208 – 215Combined sources8
Helixi218 – 223Combined sources6
Helixi228 – 250Combined sources23
Beta strandi255 – 258Combined sources4
Helixi259 – 265Combined sources7
Turni266 – 268Combined sources3
Helixi272 – 295Combined sources24
Beta strandi296 – 298Combined sources3
Beta strandi299 – 301Combined sources3
Helixi305 – 321Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L7VX-ray3.20A/B1-326[»]
2QI9X-ray2.60A/B1-326[»]
4DBLX-ray3.49A/B/F/G1-326[»]
4FI3X-ray3.47A/B1-326[»]
4R9UX-ray2.78A/B1-326[»]
ProteinModelPortaliP06609.
SMRiP06609.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06609.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108ESU. Bacteria.
COG4139. LUCA.
HOGENOMiHOG000045407.
InParanoidiP06609.
KOiK06073.
OMAiGMQARAL.
PhylomeDBiP06609.

Family and domain databases

Gene3Di1.10.3470.10. 1 hit.
HAMAPiMF_01004. BtuC. 1 hit.
InterProiIPR029022. ABC_BtuC-like.
IPR023691. ABC_transptr_BtuC.
IPR000522. ABC_transptr_FecCD.
[Graphical view]
PANTHERiPTHR30472. PTHR30472. 1 hit.
PfamiPF01032. FecCD. 1 hit.
[Graphical view]
SUPFAMiSSF81345. SSF81345. 1 hit.

Sequencei

Sequence statusi: Complete.

P06609-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTLARQQQR QNIRWLLCLS VLMLLALLLS LCAGEQWISP GDWFTPRGEL
60 70 80 90 100
FVWQIRLPRT LAVLLVGAAL AISGAVMQAL FENPLAEPGL LGVSNGAGVG
110 120 130 140 150
LIAAVLLGQG QLPNWALGLC AIAGALIITL ILLRFARRHL STSRLLLAGV
160 170 180 190 200
ALGIICSALM TWAIYFSTSV DLRQLMYWMM GGFGGVDWRQ SWLMLALIPV
210 220 230 240 250
LLWICCQSRP MNMLALGEIS ARQLGLPLWF WRNVLVAATG WMVGVSVALA
260 270 280 290 300
GAIGFIGLVI PHILRLCGLT DHRVLLPGCA LAGASALLLA DIVARLALAA
310 320
AELPIGVVTA TLGAPVFIWL LLKAGR
Length:326
Mass (Da):34,949
Last modified:November 1, 1997 - v3
Checksum:iC65882B1C47BFE69
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111 – 112QL → LT in AAA23526 (PubMed:3528129).Curated2
Sequence conflicti123A → R in AAA23526 (PubMed:3528129).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14031 Genomic DNA. Translation: AAA23526.1.
U00096 Genomic DNA. Translation: AAC74781.1.
AP009048 Genomic DNA. Translation: BAA15479.1.
PIRiG64929. QRECBC.
RefSeqiNP_416226.1. NC_000913.3.
WP_000956528.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74781; AAC74781; b1711.
BAA15479; BAA15479; BAA15479.
GeneIDi945877.
KEGGiecj:JW1701.
eco:b1711.
PATRICi32118728. VBIEscCol129921_1782.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14031 Genomic DNA. Translation: AAA23526.1.
U00096 Genomic DNA. Translation: AAC74781.1.
AP009048 Genomic DNA. Translation: BAA15479.1.
PIRiG64929. QRECBC.
RefSeqiNP_416226.1. NC_000913.3.
WP_000956528.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L7VX-ray3.20A/B1-326[»]
2QI9X-ray2.60A/B1-326[»]
4DBLX-ray3.49A/B/F/G1-326[»]
4FI3X-ray3.47A/B1-326[»]
4R9UX-ray2.78A/B1-326[»]
ProteinModelPortaliP06609.
SMRiP06609.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263152. 131 interactors.
DIPiDIP-9233N.
IntActiP06609. 2 interactors.
MINTiMINT-6803761.
STRINGi511145.b1711.

Protein family/group databases

TCDBi3.A.1.13.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP06609.
PRIDEiP06609.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74781; AAC74781; b1711.
BAA15479; BAA15479; BAA15479.
GeneIDi945877.
KEGGiecj:JW1701.
eco:b1711.
PATRICi32118728. VBIEscCol129921_1782.

Organism-specific databases

EchoBASEiEB0125.
EcoGeneiEG10127. btuC.

Phylogenomic databases

eggNOGiENOG4108ESU. Bacteria.
COG4139. LUCA.
HOGENOMiHOG000045407.
InParanoidiP06609.
KOiK06073.
OMAiGMQARAL.
PhylomeDBiP06609.

Enzyme and pathway databases

BioCyciEcoCyc:BTUC-MONOMER.
ECOL316407:JW1701-MONOMER.
MetaCyc:BTUC-MONOMER.
BRENDAi3.6.3.33. 2026.

Miscellaneous databases

EvolutionaryTraceiP06609.
PROiP06609.

Family and domain databases

Gene3Di1.10.3470.10. 1 hit.
HAMAPiMF_01004. BtuC. 1 hit.
InterProiIPR029022. ABC_BtuC-like.
IPR023691. ABC_transptr_BtuC.
IPR000522. ABC_transptr_FecCD.
[Graphical view]
PANTHERiPTHR30472. PTHR30472. 1 hit.
PfamiPF01032. FecCD. 1 hit.
[Graphical view]
SUPFAMiSSF81345. SSF81345. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBTUC_ECOLI
AccessioniPrimary (citable) accession number: P06609
Secondary accession number(s): P77197
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.