Reviewed, UniProtKB/Swiss-Prot P06596 (PA21B_CANFA)

Names and origin

Protein names

Recommended name:
    Phospholipase A2
    EC=3.1.1.4
Alternative name(s):
    Phosphatidylcholine 2-acylhydrolase
    Group IB phospholipase A2

Gene names

Name:

PLA2G1B

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the phospholipase A2 family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 22	7			PRO_0000022733
	Chain	23 – 146	124	Phospholipase A2		PRO_0000022734
Sites
	Active site	70	1	By similarity
	Active site	121	1	By similarity
	Metal binding	50	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	52	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	54	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	71	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	33 ↔ 99		By similarity
	Disulfide bond	49 ↔ 146		By similarity
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	66 ↔ 127		By similarity
	Disulfide bond	73 ↔ 120		By similarity
	Disulfide bond	83 ↔ 113		By similarity
	Disulfide bond	106 ↔ 118		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06596-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: F6258ED9527F3692	FASTA	146	16,236
	10 20 30 40 50 60 MKFLVLAALL TVAAAEGGIS PRAVWQFRNM IKCTIPESDP LKDYNDYGCY CGLGGSGTPV 70 80 90 100 110 120 DELDKCCQTH DHCYSEAKKL DSCKFLLDNP YTKIYSYSCS GSEITCSSKN KDCQAFICNC 130 140 DRSAAICFSK APYNKEHKNL DTKKYC

References

[1]	"Dog and rat pancreatic phospholipases A2: complete amino acid sequences deduced from complementary DNAs." Ohara O., Tamaki M., Nakamura E., Tsuruta Y., Fujii Y., Shin M., Teraoka H., Okamoto M. J. Biochem. 99:733-739(1986) [PubMed: 3754861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and sequence of the canine pancreatic phospholipase A2 gene." Kerfelec B., Laforge K.S., Vasiloudes P., Puigserver A., Scheele G.A. Eur. J. Biochem. 190:299-304(1990) [PubMed: 2142076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Primary structures of canine pancreatic lipase and phospholipase A2 messenger RNAs." Kerfelec B., Laforge K.S., Puigserver A., Scheele G.A. Pancreas 1:430-437(1986) [PubMed: 3562437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D00035 mRNA. Translation: BAA00023.1. M35301 mRNA. Translation: AAA30883.1.
PIR	PSDG. S11316.
RefSeq	NP_001003320.1.
UniGene	Cfa.40293
3D structure databases
HSSP	HSSP built from PDB template 1HN4 based on UniProtKB P00592.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFG00000010263. Canis familiaris. [Contig view]
GeneID	404011.
KEGG	cfa:404011.
Phylogenomic databases
HOVERGEN	P06596.
OMA	P06596. AKKLDSC.
Enzyme and pathway databases
BRENDA	3.1.1.4. 463.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
ProDom	PD000303. PhospholipaseA2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA21B_CANFA

Accession

Primary (citable) accession number: P06596

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)