Reviewed, UniProtKB/Swiss-Prot P06573 (ERM_STRSA)

Names and origin

Protein names	Recommended name:     rRNA adenine N-6-methyltransferase     EC=2.1.1.48 Alternative name(s):     Macrolide-lincosamide-streptogramin B resistance protein
Encoded on	Plasmid pAM77
Organism	Streptococcus sanguis
Taxonomic identifier	1305 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein produces a dimethylation of the adenine residue at position 2058 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine.
Sequence similarities	Belongs to the rRNA adenine N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	rRNA adenine N-6-methyltransferase		PRO_0000101691

Sequences

	Sequence		Length	Mass (Da)
	P06573-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 8763C133D73309A5	FASTA	245	28,796
	10 20 30 40 50 60 MKKNIKYSQN FLTNEKVLNQ IIKQLNLKET DTVYEIGTGK GHLTTKLAKI SKQVTSIELD 70 80 90 100 110 120 SHLFNLSSEK LKLNIRVTLI HQDILQFQFP NKQRYKIVGS IPYHLSTQII KKVVFESHAS 130 140 150 160 170 180 DIYLIVEEGF YKRTLDIHRS LGLLLHTQVS IQQLLKLPAE CFHPKPKVNS VLIKLTRHTT 190 200 210 220 230 240 DVPDKYWKLY TYFVSKWVNR EYRQLFTKNQ FHQAMKHAKV NNLSTVTYEQ VLSIFNSYLL FNGRK

References

[1]	"A complex attenuator regulates inducible resistance to macrolides, lincosamides, and streptogramin type B antibiotics in Streptococcus sanguis." Horinouchi S., Byeon W.-H., Weisblum B. J. Bacteriol. 154:1252-1262(1983) [PubMed: 6406429] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	K00551 Genomic DNA. Translation: AAA25527.1.
3D structure databases
HSSP	HSSP built from PDB template 1YUB based on UniProtKB P21236.
SMR	P06573. Positions 1-245.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.48. 18606.
Family and domain databases
InterPro	IPR001737. RRNA_Ade_Mease_Trfase. [Graphical view]
PANTHER	PTHR11727. RRNA_meth_trans. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERM_STRSA

Accession

Primary (citable) accession number: P06573

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	March 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)