P06572 (ERMM_STAEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
rRNA adenine N-6-methyltransferase
EC=2.1.1.184

Alternative name(s):
Erythromycin resistance protein
Macrolide-lincosamide-streptogramin B resistance protein

Gene names

Name:

ermM

Encoded on

Plasmid pNE131

Organism

Staphylococcus epidermidis

Taxonomic identifier

1282 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein produces a dimethylation of the adenine residue at position 2085 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
Catalytic activity	2 S-adenosyl-L-methionine + adenine(2085) in 23S rRNA = 2 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(2085) in 23S rRNA.
Sequence similarities	Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	rRNA adenine N-6-methyltransferase		PRO_0000101685
Sites
	Binding site	11	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	13	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	38	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	59	1	S-adenosyl-L-methionine By similarity
	Binding site	84	1	S-adenosyl-L-methionine By similarity
	Binding site	101	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06572 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 765404D860B6D620	FASTA	244	28,941
	10 20 30 40 50 60 MNEKNIKHSQ NFITSKHNID KIMTNIRLNE HDNIFEIGSG KGHFTLELVQ RCNFVTAIEI 70 80 90 100 110 120 DHKLCKTTEN KLVDHDNFQV LNKDILQFKF PKNQSYKIFG NIPYNISTDI IRKIVFDSIA 130 140 150 160 170 180 DEIYLIVEYG FAKRLLNTKR SFALFLMAEV DISILSMVPR EYFHPKPKVN SSLIRLNRKK 190 200 210 220 230 240 SRISHKDKQK YNYFVMKWVN KEYKKIFTKN QFNNSLKHAG IDDLNNISFE QFLSLFNSYK LFNK

References

[1]	"Nucleotide sequence of the constitutive macrolide-lincosamide-streptogramin B resistance plasmid pNE131 from Staphylococcus epidermidis and homologies with Staphylococcus aureus plasmids pE194 and pSN2." Lampson B.C., Parisi J.T. J. Bacteriol. 167:888-892(1986) [PubMed: 3091582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Naturally occurring Staphylococcus epidermidis plasmid expressing constitutive macrolide-lincosamide-streptogramin B resistance contains a deleted attenuator." Lampson B.C., Parisi J.T. J. Bacteriol. 166:479-483(1986) [PubMed: 3084450] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12730 Genomic DNA. Translation: AAA98296.1.
PIR	A24497.
3D structure databases
ProteinModelPortal	P06572.
SMR	P06572. Positions 9-244.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
ProtClustDB	CLSK884354.
Family and domain databases
InterPro	IPR023165. rRNA_Ade_diMease-like. IPR020596. rRNA_Ade_Mease_Trfase_CS. IPR001737. rRNA_Ade_methylase_transferase. IPR020598. rRNA_Ade_methylase_Trfase_N. [Graphical view]
Gene3D	G3DSA:1.10.8.100. rRNA_Ade_diMease-like. 1 hit.
PANTHER	PTHR11727. RRNA_meth_trans. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERMM_STAEP

Accession

Primary (citable) accession number: P06572

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	May 31, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program