Reviewed, UniProtKB/Swiss-Prot P06571 (ERMG_BACSH)

Names and origin

Protein names

Recommended name:
    rRNA adenine N-6-methyltransferase
    EC=2.1.1.48
Alternative name(s):
    Macrolide-lincosamide-streptogramin B resistance protein
    Erythromycin resistance protein

Gene names

Name:

ermG

Organism

Bacillus sphaericus

Taxonomic identifier

1421 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Planococcaceae › Lysinibacillus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein produces a dimethylation of the adenine residue at position 2058 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine.
Sequence similarities	Belongs to the rRNA adenine N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	rRNA adenine N-6-methyltransferase		PRO_0000101673

Sequences

	Sequence		Length	Mass (Da)
	P06571-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: E64A1714B32225EF	FASTA	244	28,557
	10 20 30 40 50 60 MNKVNIKDSQ NFITSKYHIE KIMNCISLDE KDNIFEIGAG KGHFTAELVK RCNFVTAIEI 70 80 90 100 110 120 DSKLCEVTRN KLLNYPNYQI VNDDILKFTF PSHNPYKIFG SIPYNISTNI IRKIVFESSA 130 140 150 160 170 180 TISYLIVEYG FAKRLLDTNR SLALLLMAEV DISILAKIPR YYFHPKPKVD SALIVLKRKP 190 200 210 220 230 240 AKMAFKERKK YETFVMKWVN KEYEKLFTKN QFNKALKHAR IYDINNISFE QFVSLFNSYK IFNG

References

[1]	"Cloning and analysis of ermG, a new macrolide-lincosamide-streptogramin B resistance element from Bacillus sphaericus." Monod M., Mohan S., Dubnau D. J. Bacteriol. 169:340-350(1987) [PubMed: 3025178] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M15332 Genomic DNA. Translation: AAA22419.1.
PIR	C26930.
3D structure databases
HSSP	HSSP built from PDB template 1QAN based on UniProtKB P13956.
SMR	P06571. Positions 9-243.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.48. 327.
Family and domain databases
InterPro	IPR001737. RRNA_Ade_Mease_Trfase. [Graphical view]
PANTHER	PTHR11727. RRNA_meth_trans. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERMG_BACSH

Accession

Primary (citable) accession number: P06571

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	March 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)