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P06567

- DNAI_BACSU

UniProt

P06567 - DNAI_BACSU

Protein

Primosomal protein DnaI

Gene

dnaI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    Probably involved in DNA replication.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi168 – 1758ATPCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. nucleoside-triphosphatase activity Source: InterPro
    3. protein binding Source: IntAct

    GO - Biological processi

    1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU28980-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Primosomal protein DnaI
    Gene namesi
    Name:dnaI
    Synonyms:ytxA
    Ordered Locus Names:BSU28980
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU28980. [Micado]

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 311311Primosomal protein DnaIPRO_0000114313Add
    BLAST

    Proteomic databases

    PaxDbiP06567.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    dnaCP374694EBI-2122847,EBI-2122822

    Protein-protein interaction databases

    IntActiP06567. 4 interactions.
    STRINGi224308.BSU28980.

    Structurei

    Secondary structure

    1
    311
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi16 – 2813
    Helixi32 – 409
    Turni42 – 443
    Helixi47 – 526
    Helixi54 – 629
    Turni68 – 703
    Beta strandi83 – 908
    Beta strandi93 – 1008
    Helixi102 – 1043

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2K7RNMR-A1-106[»]
    4M4WX-ray6.10J/K/L/M/N/O1-311[»]
    ProteinModelPortaliP06567.
    SMRiP06567. Positions 113-304.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP06567.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG1484.
    HOGENOMiHOG000020545.
    KOiK11144.
    OMAiILQARMD.
    OrthoDBiEOG6HXJ30.
    PhylomeDBiP06567.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR009928. DnaI_N.
    IPR002611. IstB_ATP-bd.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF07319. DnaI_N. 1 hit.
    PF01695. IstB_IS21. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P06567-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPIGRSLQG VTGRPDFQKR LEQMKEKVMK DQDVQAFLKE NEEVIDQKMI    50
    EKSLNKLYEY IEQSKNCSYC SEDENCNNLL EGYHPKLVVN GRSIDIEYYE 100
    CPVKRKLDQQ KKQQSLMKSM YIQQDLLGAT FQQVDISDPS RLAMFQHVTD 150
    FLKSYNETGK GKGLYLYGKF GVGKTFMLAA IANELAEKEY SSMIVYVPEF 200
    VRELKNSLQD QTLEEKLNMV KTTPVLMLDD IGAESMTSWV RDEVIGTVLQ 250
    HRMSQQLPTF FSSNFSPDEL KHHFTYSQRG EKEEVKAARL MERILYLAAP 300
    IRLDGENRRH P 311
    Length:311
    Mass (Da):36,114
    Last modified:January 1, 1988 - v1
    Checksum:iA86FC94AB6841264
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191K → N in AAA22405. (PubMed:3027697)Curated
    Sequence conflicti24 – 241M → T in AAA22405. (PubMed:3027697)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04963 Genomic DNA. Translation: CAA28633.1.
    Z75208 Genomic DNA. Translation: CAA99605.1.
    AF008220 Genomic DNA. Translation: AAC00359.1.
    AL009126 Genomic DNA. Translation: CAB14858.1.
    M15183 Genomic DNA. Translation: AAA22405.1.
    PIRiB24720. IQBS44.
    RefSeqiNP_390776.1. NC_000964.3.
    WP_003229466.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14858; CAB14858; BSU28980.
    GeneIDi936873.
    KEGGibsu:BSU28980.
    PATRICi18977688. VBIBacSub10457_3032.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04963 Genomic DNA. Translation: CAA28633.1 .
    Z75208 Genomic DNA. Translation: CAA99605.1 .
    AF008220 Genomic DNA. Translation: AAC00359.1 .
    AL009126 Genomic DNA. Translation: CAB14858.1 .
    M15183 Genomic DNA. Translation: AAA22405.1 .
    PIRi B24720. IQBS44.
    RefSeqi NP_390776.1. NC_000964.3.
    WP_003229466.1. NZ_CM000487.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2K7R NMR - A 1-106 [» ]
    4M4W X-ray 6.10 J/K/L/M/N/O 1-311 [» ]
    ProteinModelPortali P06567.
    SMRi P06567. Positions 113-304.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P06567. 4 interactions.
    STRINGi 224308.BSU28980.

    Proteomic databases

    PaxDbi P06567.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14858 ; CAB14858 ; BSU28980 .
    GeneIDi 936873.
    KEGGi bsu:BSU28980.
    PATRICi 18977688. VBIBacSub10457_3032.

    Organism-specific databases

    GenoListi BSU28980. [Micado ]

    Phylogenomic databases

    eggNOGi COG1484.
    HOGENOMi HOG000020545.
    KOi K11144.
    OMAi ILQARMD.
    OrthoDBi EOG6HXJ30.
    PhylomeDBi P06567.

    Enzyme and pathway databases

    BioCyci BSUB:BSU28980-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P06567.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR009928. DnaI_N.
    IPR002611. IstB_ATP-bd.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF07319. DnaI_N. 1 hit.
    PF01695. IstB_IS21. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and organization of dnaB gene and neighbouring genes on the Bacillus subtilis chromosome."
      Ogasawara N., Moriya S., Mazza P.G., Yoshikawa H.
      Nucleic Acids Res. 14:9989-9999(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism."
      Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.
      Microbiology 142:3067-3078(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
      Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
      Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    5. "Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA replication initiation and membrane attachment."
      Hoshino T., McKenzie T., Schmidt S., Tanaka T., Sueoka N.
      Proc. Natl. Acad. Sci. U.S.A. 84:653-657(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-206.
    6. "Archaebacterial virus SSV1 encodes a putative DnaA-like protein."
      Koonin E.V.
      Nucleic Acids Res. 20:1143-1143(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY TO DNAA.
    7. "The Bacillus subtilis dnaI gene is part of the dnaB operon."
      Bruand C., Ehrlich S.D.
      Microbiology 141:1199-1200(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiDNAI_BACSU
    AccessioniPrimary (citable) accession number: P06567
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3