P06567 (DNAI_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Primosomal protein DnaI

Gene names

Name:	dnaI
Synonyms:	ytxA
Ordered Locus Names:	BSU28980

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Probably involved in DNA replication.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Ligand	ATP-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dnaC	P37469	4	EBI-2122847,EBI-2122822

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 311

311

Primosomal protein DnaI

PRO_0000114313

Regions

Nucleotide binding

168 – 175

8

ATP Probable

Experimental info

Sequence conflict

19

1

K → N in AAA22405. Ref.5

Sequence conflict

24

1

M → T in AAA22405. Ref.5

Secondary structure

1

.

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311

Helix Strand Turn

Details...

Helix

16 – 28

13

Helix

32 – 40

9

Turn

42 – 44

3

Helix

47 – 52

6

Helix

54 – 62

9

Turn

68 – 70

3

Beta strand

83 – 90

8

Beta strand

93 – 100

8

Helix

102 – 104

3

Sequences

	Sequence		Length	Mass (Da)
	P06567 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: A86FC94AB6841264	FASTA	311	36,114
	10 20 30 40 50 60 MEPIGRSLQG VTGRPDFQKR LEQMKEKVMK DQDVQAFLKE NEEVIDQKMI EKSLNKLYEY 70 80 90 100 110 120 IEQSKNCSYC SEDENCNNLL EGYHPKLVVN GRSIDIEYYE CPVKRKLDQQ KKQQSLMKSM 130 140 150 160 170 180 YIQQDLLGAT FQQVDISDPS RLAMFQHVTD FLKSYNETGK GKGLYLYGKF GVGKTFMLAA 190 200 210 220 230 240 IANELAEKEY SSMIVYVPEF VRELKNSLQD QTLEEKLNMV KTTPVLMLDD IGAESMTSWV 250 260 270 280 290 300 RDEVIGTVLQ HRMSQQLPTF FSSNFSPDEL KHHFTYSQRG EKEEVKAARL MERILYLAAP 310 IRLDGENRRH P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and organization of dnaB gene and neighbouring genes on the Bacillus subtilis chromosome." Ogasawara N., Moriya S., Mazza P.G., Yoshikawa H. Nucleic Acids Res. 14:9989-9999(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region." Lapidus A., Galleron N., Sorokin A., Ehrlich S.D. Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[4]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[5]	"Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA replication initiation and membrane attachment." Hoshino T., McKenzie T., Schmidt S., Tanaka T., Sueoka N. Proc. Natl. Acad. Sci. U.S.A. 84:653-657(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-206.
[6]	"Archaebacterial virus SSV1 encodes a putative DnaA-like protein." Koonin E.V. Nucleic Acids Res. 20:1143-1143(1992) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY TO DNAA.
[7]	"The Bacillus subtilis dnaI gene is part of the dnaB operon." Bruand C., Ehrlich S.D. Microbiology 141:1199-1200(1995) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X04963 Genomic DNA. Translation: CAA28633.1.
Z75208 Genomic DNA. Translation: CAA99605.1.
AF008220 Genomic DNA. Translation: AAC00359.1.
AL009126 Genomic DNA. Translation: CAB14858.1.
M15183 Genomic DNA. Translation: AAA22405.1.

PIR

IQBS44. B24720.

RefSeq

NP_390776.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2K7R	NMR	-	A	1-106	[»]

ProteinModelPortal

P06567.

SMR

P06567. Positions 113-304.

ModBase

Search...

Protein-protein interaction databases

IntAct

P06567. 4 interactions.

STRING

224308.BSU28980.

Proteomic databases

PaxDb

P06567.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB14858; CAB14858; BSU28980.

GeneID

936873.

KEGG

bsu:BSU28980.

PATRIC

18977688. VBIBacSub10457_3032.

Organism-specific databases

GenoList

BSU28980. [Micado]

Phylogenomic databases

eggNOG

COG1484.

HOGENOM

HOG000020545.

KO

K11144.

OMA

ILQARMD.

ProtClustDB

PRK08939.

Enzyme and pathway databases

BioCyc

BSUB:BSU28980-MONOMER.

Family and domain databases

InterPro

IPR003593. AAA+_ATPase.
IPR009928. DnaI_N.
IPR002611. IstB_ATP-bd.
[Graphical view]

Pfam

PF07319. DnaI_N. 1 hit.
PF01695. IstB_IS21. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P06567.

Entry information

Entry name

DNAI_BACSU

Accession

Primary (citable) accession number: P06567

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program