P06565 (GUN2_BACS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase B
EC=3.2.1.4

Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B

Gene names

Name:

celB

Organism

Bacillus sp. (strain N-4 / JCM 9156)

Taxonomic identifier

1413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Endoglucanase B		PRO_0000184045
Sites
	Active site	165	1	Proton donor By similarity
	Active site	254	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06565 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 9888660E66B4DA3F	FASTA	409	45,690
	10 20 30 40 50 60 MKKITTIFVV LLMTLALFII GNTTAADDYS VVEEHGQLSI SNGELVNDRG EPVQLKGMSS 70 80 90 100 110 120 HGLQWYGQFV NYESMKWLRD DWGITVFRAA MYTSSGGYIE DPSVKEKVKE AVEAAIDLGI 130 140 150 160 170 180 YVIIDWHILS DNDPNIYKEE AKDFFDEMSE LYGDYPNVIY EIANEPNGSD VTWDNQIKPY 190 200 210 220 230 240 AEEVIPVIRN NDPNNIIIVG TGTWSQDVHH AADNQLTDPN VMYAFHFYAG THGQNLRDQV 250 260 270 280 290 300 DYALDQGAAI FVSEWGTSEA TGDGGVFLDE AQVWIDFMDE RNLSWANWSL THKDESSAAL 310 320 330 340 350 360 MPGASPTGGW TEAELSPSGT FVREKIRESA TTPPSDPTPP SDPDPGEPEP DPGEPDPTPP 370 380 390 400 SDPGDYPAWD PNTIYTDEIV YHNGQLWQAK WWTQNQEPGD PYGPWEPLN

References

[1]	"Nucleotide sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology." Fukumori F., Sashihara N., Kudo T., Horikoshi K. J. Bacteriol. 168:479-485(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14729 Genomic DNA. Translation: AAA22299.1.
PIR	B25156.
3D structure databases
ProteinModelPortal	P06565.
SMR	P06565. Positions 30-331.
ModBase	Search...
Protein family/group databases
CAZy	CBM5. Carbohydrate-Binding Module Family 5. GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.10.10.20. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR003610. CBM_fam5/12. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF02839. CBM_5_12. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view]
SMART	SM00495. ChtBD3. 1 hit. [Graphical view]
SUPFAM	SSF51055. CBM_5_12. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN2_BACS4

Accession

Primary (citable) accession number: P06565

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program