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Protein

Endoglucanase B

Gene

celB

Organism
Bacillus cellulosilyticus (strain ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei165 – 1651Proton donorBy similarity
Active sitei254 – 2541NucleophileBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM5. Carbohydrate-Binding Module Family 5.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase B (EC:3.2.1.4)
Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Gene namesi
Name:celB
OrganismiBacillus cellulosilyticus (strain ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4)
Taxonomic identifieri649639 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409Endoglucanase BPRO_0000184045Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP06565.
SMRiP06565. Positions 30-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.10.10.20. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR003610. CBM_fam5/12.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02839. CBM_5_12. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTiSM00495. ChtBD3. 1 hit.
[Graphical view]
SUPFAMiSSF51055. SSF51055. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06565-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKITTIFVV LLMTLALFII GNTTAADDYS VVEEHGQLSI SNGELVNDRG
60 70 80 90 100
EPVQLKGMSS HGLQWYGQFV NYESMKWLRD DWGITVFRAA MYTSSGGYIE
110 120 130 140 150
DPSVKEKVKE AVEAAIDLGI YVIIDWHILS DNDPNIYKEE AKDFFDEMSE
160 170 180 190 200
LYGDYPNVIY EIANEPNGSD VTWDNQIKPY AEEVIPVIRN NDPNNIIIVG
210 220 230 240 250
TGTWSQDVHH AADNQLTDPN VMYAFHFYAG THGQNLRDQV DYALDQGAAI
260 270 280 290 300
FVSEWGTSEA TGDGGVFLDE AQVWIDFMDE RNLSWANWSL THKDESSAAL
310 320 330 340 350
MPGASPTGGW TEAELSPSGT FVREKIRESA TTPPSDPTPP SDPDPGEPEP
360 370 380 390 400
DPGEPDPTPP SDPGDYPAWD PNTIYTDEIV YHNGQLWQAK WWTQNQEPGD

PYGPWEPLN
Length:409
Mass (Da):45,690
Last modified:January 1, 1988 - v1
Checksum:i9888660E66B4DA3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14729 Genomic DNA. Translation: AAA22299.1.
PIRiB25156.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14729 Genomic DNA. Translation: AAA22299.1.
PIRiB25156.

3D structure databases

ProteinModelPortaliP06565.
SMRiP06565. Positions 30-331.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM5. Carbohydrate-Binding Module Family 5.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.10.10.20. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR003610. CBM_fam5/12.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02839. CBM_5_12. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTiSM00495. ChtBD3. 1 hit.
[Graphical view]
SUPFAMiSSF51055. SSF51055. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology."
    Fukumori F., Sashihara N., Kudo T., Horikoshi K.
    J. Bacteriol. 168:479-485(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGUN2_BACCJ
AccessioniPrimary (citable) accession number: P06565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 1, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.