P06564 (GUN_BACS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endoglucanase EC=3.2.1.4 Alternative name(s): Alkaline cellulase Endo-1,4-beta-glucanase
Organism	Bacillus sp. (strain 1139)
Taxonomic identifier	1411 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	800 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Miscellaneous	Alkalophilic Bacillus sp strain 1139 is not a true cellulolytic micro-organism because the enzyme is unable to hydrolyze native cellulose.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Biophysicochemical properties	pH dependence: Optimum pH is 9.0.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 30

30

Potential

Chain

31 – 800

770

Endoglucanase

PRO_0000007837

Sites

Active site

190

1

Proton donor By similarity

Active site

305

1

Nucleophile By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

800

Helix Strand Turn

Details...

Beta strand

588 – 590

3

Beta strand

594 – 596

3

Beta strand

600 – 602

3

Beta strand

613 – 617

5

Beta strand

620 – 628

9

Turn

637 – 640

4

Beta strand

643 – 647

5

Beta strand

658 – 669

12

Beta strand

674 – 681

8

Helix

684 – 686

3

Beta strand

696 – 699

4

Helix

700 – 705

6

Beta strand

712 – 720

9

Beta strand

735 – 744

10

Beta strand

748 – 759

12

Sequences

	Sequence		Length	Mass (Da)
	P06564 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 7CCA4D7B6DAD55CF	FASTA	800	88,602
	10 20 30 40 50 60 MMLRKKTKQL ISSILILVLL LSLFPTALAA EGNTREDNFK HLLGNDNVKR PSEAGALQLQ 70 80 90 100 110 120 EVDGQMTLVD QHGEKIQLRG MSTHGLQWFP EILNDNAYKA LANDWESNMI RLAMYVGENG 130 140 150 160 170 180 YASNPELIKS RVIKGIDLAI ENDMYVIVDW HVHAPGDPRD PVYAGAEDFF RDIAALYPNN 190 200 210 220 230 240 PHIIYELANE PSSNNNGGAG IPNNEEGWNA VKEYADPIVE MLRDSGNADD NIIIVGSPNW 250 260 270 280 290 300 SQRPDLAADN PIDDHHTMYT VHFYTGSHAA STESYPPETP NSERGNVMSN TRYALENGVA 310 320 330 340 350 360 VFATEWGTSQ ANGDGGPYFD EADVWIEFLN ENNISWANWS LTNKNEVSGA FTPFELGKSN 370 380 390 400 410 420 ATSLDPGPDQ VWVPEELSLS GEYVRARIKG VNYEPIDRTK YTKVLWDFND GTKQGFGVNG 430 440 450 460 470 480 DSPVEDVVIE NEAGALKLSG LDASNDVSEG NYWANARLSA DGWGKSVDIL GAEKLTMDVI 490 500 510 520 530 540 VDEPTTVSIA AIPQGPSANW VNPNRAIKVE PTNFVPLEDK FKAELTITSA DSPSLEAIAM 550 560 570 580 590 600 HAENNNINNI ILFVGTEGAD VIYLDNIKVI GTEVEIPVVH DPKGEAVLPS VFEDGTRQGW 610 620 630 640 650 660 DWAGESGVKT ALTIEEANGS NALSWEFGYP EVKPSDNWAT APRLDFWKSD LVRGENDYVT 670 680 690 700 710 720 FDFYLDPVRA TEGAMNINLV FQPPTNGYWV QAPKTYTINF DELEEPNQVN GLYHYEVKIN 730 740 750 760 770 780 VRDITNIQDD TLLRNMMIIF ADVESDFAGR VFVDNVRFEG AATTEPVEPE PVDPGEETPP 790 800 VDEKEAKTEQ KEAEKEEKEE

References

[1]	"Molecular cloning and nucleotide sequence of the alkaline cellulase gene from the alkalophilic Bacillus sp. strain 1139." Fukumori F., Kudo T., Narahashi Y., Horikoshi K. J. Gen. Microbiol. 132:2329-2335(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M15743 Genomic DNA. Translation: AAA22305.1.
D00066 Genomic DNA. Translation: BAA00045.1.

PIR

A29003.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UWW	X-ray	1.40	A/B	571-761	[»]

ProteinModelPortal

P06564.

SMR

P06564. Positions 44-570, 578-761.

ModBase

Search...

Protein family/group databases

CAZy

CBM17. Carbohydrate-Binding Module Family 17.
CBM28. Carbohydrate-Binding Module Family 28.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.20.20.80. 1 hit.

InterPro

IPR005086. CBM_fam_17/28.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Pfam

PF03424. CBM_17_28. 2 hits.
PF00150. Cellulase. 1 hit.
[Graphical view]

SUPFAM

SSF49785. Gal_bind_like. 2 hits.
SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P06564.

Entry information

Entry name

GUN_BACS1

Accession

Primary (citable) accession number: P06564

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program