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P06564 (GUN_BACA3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase

EC=3.2.1.4
Alternative name(s):
Alkaline cellulase
Endo-1,4-beta-glucanase
OrganismBacillus akibai (strain ATCC 43226 / DSM 21942 / JCM 9157 / 1139)
Taxonomic identifier1236973 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length800 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Miscellaneous

Alkalophilic Bacillus sp strain 1139 is not a true cellulolytic micro-organism because the enzyme is unable to hydrolyze native cellulose.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Biophysicochemical properties

pH dependence:

Optimum pH is 9.0.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 800770Endoglucanase
PRO_0000007837

Sites

Active site1901Proton donor By similarity
Active site3051Nucleophile By similarity

Secondary structure

.............................. 800
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06564 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 7CCA4D7B6DAD55CF

FASTA80088,602
        10         20         30         40         50         60 
MMLRKKTKQL ISSILILVLL LSLFPTALAA EGNTREDNFK HLLGNDNVKR PSEAGALQLQ 

        70         80         90        100        110        120 
EVDGQMTLVD QHGEKIQLRG MSTHGLQWFP EILNDNAYKA LANDWESNMI RLAMYVGENG 

       130        140        150        160        170        180 
YASNPELIKS RVIKGIDLAI ENDMYVIVDW HVHAPGDPRD PVYAGAEDFF RDIAALYPNN 

       190        200        210        220        230        240 
PHIIYELANE PSSNNNGGAG IPNNEEGWNA VKEYADPIVE MLRDSGNADD NIIIVGSPNW 

       250        260        270        280        290        300 
SQRPDLAADN PIDDHHTMYT VHFYTGSHAA STESYPPETP NSERGNVMSN TRYALENGVA 

       310        320        330        340        350        360 
VFATEWGTSQ ANGDGGPYFD EADVWIEFLN ENNISWANWS LTNKNEVSGA FTPFELGKSN 

       370        380        390        400        410        420 
ATSLDPGPDQ VWVPEELSLS GEYVRARIKG VNYEPIDRTK YTKVLWDFND GTKQGFGVNG 

       430        440        450        460        470        480 
DSPVEDVVIE NEAGALKLSG LDASNDVSEG NYWANARLSA DGWGKSVDIL GAEKLTMDVI 

       490        500        510        520        530        540 
VDEPTTVSIA AIPQGPSANW VNPNRAIKVE PTNFVPLEDK FKAELTITSA DSPSLEAIAM 

       550        560        570        580        590        600 
HAENNNINNI ILFVGTEGAD VIYLDNIKVI GTEVEIPVVH DPKGEAVLPS VFEDGTRQGW 

       610        620        630        640        650        660 
DWAGESGVKT ALTIEEANGS NALSWEFGYP EVKPSDNWAT APRLDFWKSD LVRGENDYVT 

       670        680        690        700        710        720 
FDFYLDPVRA TEGAMNINLV FQPPTNGYWV QAPKTYTINF DELEEPNQVN GLYHYEVKIN 

       730        740        750        760        770        780 
VRDITNIQDD TLLRNMMIIF ADVESDFAGR VFVDNVRFEG AATTEPVEPE PVDPGEETPP 

       790        800 
VDEKEAKTEQ KEAEKEEKEE 

« Hide

References

[1]"Molecular cloning and nucleotide sequence of the alkaline cellulase gene from the alkalophilic Bacillus sp. strain 1139."
Fukumori F., Kudo T., Narahashi Y., Horikoshi K.
J. Gen. Microbiol. 132:2329-2335(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M15743 Genomic DNA. Translation: AAA22305.1.
D00066 Genomic DNA. Translation: BAA00045.1.
PIRA29003.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UWWX-ray1.40A/B571-761[»]
ProteinModelPortalP06564.
SMRP06564. Positions 44-570, 578-761.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM17. Carbohydrate-Binding Module Family 17.
CBM28. Carbohydrate-Binding Module Family 28.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProIPR005086. CBM_fam_17/28.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF03424. CBM_17_28. 2 hits.
PF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP06564.

Entry information

Entry nameGUN_BACA3
AccessionPrimary (citable) accession number: P06564
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: May 14, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries