Reviewed, UniProtKB/Swiss-Prot P06557 (TRPE_CORGL)

Names and origin

Protein names

Recommended name:
    Anthranilate synthase component 1
    EC=4.1.3.27
Alternative name(s):
    Anthranilate synthase component I

Gene names

Name:	trpE
Ordered Locus Names:	Cgl3029, cg3359

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Anthranilate synthase component 1		PRO_0000154091
Experimental info
	Sequence conflict	16	1	E → A in AAB59111. Ref.6
	Sequence conflict	185	1	A → T Ref.1
	Sequence conflict	185	1	A → T Ref.2
	Sequence conflict	185	1	A → T Ref.5
	Sequence conflict	185	1	A → T Ref.6
	Sequence conflict	357 – 358	2	EH → DD Ref.1
	Sequence conflict	357 – 358	2	EH → DD Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P06557-1 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: 0662D4A660C16FF5	FASTA	518	56,384
	10 20 30 40 50 60 MSTNPHVFSL DVRYHEDASA LFAHLGGTTA DDAALLESAD ITTKNGISSL AVLKSSVRIT 70 80 90 100 110 120 CTGNTVVTQP LTDSGRAVVA RLTQQLGQYN TAENTFSFPA SDAVDERERL TAPSTIEVLR 130 140 150 160 170 180 KLQFESGYSD ASLPLLMGGF AFDFLETFET LPAVEESVNT YPDYQFVLAE IVLDINHQDQ 190 200 210 220 230 240 TAKLAGVSNA PGELEAELNK LSLLIDAALP ATEHAYQTTP HDGDTLRVVA DIPDAQFRTQ 250 260 270 280 290 300 INELKENIYN GDIYQVVPAR TFTAPCPDAF AAYLQLRATN PSPYMFYIRG LNEGRSYELF 310 320 330 340 350 360 GASPESNLKF TAANRELQLY PIAGTRPRGL NPDGSINDEL DIRNELDMRT DAKEIAEHTM 370 380 390 400 410 420 LVDLARNDLA RVSVPASRRV ADLLQVDRYS RVMHLVSRVT ATLDPELDAL DAYRACMNMG 430 440 450 460 470 480 TLTGAPKLRA MELLRGVEKR RRGSYGGAVG YLRGNGDMDN CIVIRSAFVQ DGVAAVQAGA 490 500 510 GVVRDSNPQS EADETLHKAY AVLNAIALAA GSTLEVIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide and deduced amino acid sequences of the Brevibacterium lactofermentum tryptophan operon." Matsui K., Sano K., Ohtsubo E. Nucleic Acids Res. 14:10113-10114(1986) [PubMed: 3808947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the Corynebacterium glutamicum trpE gene." Heery D.M., Dunican L.K. Nucleic Acids Res. 18:7138-7138(1990) [PubMed: 2263476] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[3]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[5]	"Structure and function of the trp operon control regions of Brevibacterium lactofermentum, a glutamic-acid-producing bacterium." Sano K., Matsui K. Gene 53:191-200(1987) [PubMed: 3609747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-275.
[6]	"Two single-base-pair substitutions causing desensitization to tryptophan feedback inhibition of anthranilate synthase and enhanced expression of tryptophan genes of Brevibacterium lactofermentum." Matsui K., Miwa K., Sano K. J. Bacteriol. 169:5330-5332(1987) [PubMed: 3667535] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-201.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04960 Genomic DNA. Translation: CAA28623.1. X55994 Genomic DNA. Translation: CAA39467.1. BA000036 Genomic DNA. Translation: BAC00424.1. BX927157 Genomic DNA. Translation: CAF18969.1. M16663 Genomic DNA. Translation: AAA83989.1. M17892 Genomic DNA. Translation: AAB59111.1.
PIR	B24723.
RefSeq	NP_602223.1. YP_227280.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1020972. 3345521.
GenomeReviews	Gene locus Cgl3029 in contig BA000036_GR.
KEGG	cgb:cg3359. cgl:NCgl2927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507440.
OMA	RIYEWEN.
PhylomeDB	P06557.
Enzyme and pathway databases
BioCyc	CGLU196627:CG3359-MONOMER.
BRENDA	4.1.3.27. 812.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR006805. Anth_synth_I_N. IPR019999. Anthranilate_synth_I_C. IPR015890. Chorismate-bd_C. IPR005257. TrpE_synth. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
PANTHER	PTHR11236. TRPE_1_chor_bd. 1 hit.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
TIGRFAMs	TIGR00565. trpE_proteo. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_CORGL

Accession

Primary (citable) accession number: P06557

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	July 11, 2002
Last modified:	February 9, 2010
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)