P06548 (BLA3_BACCE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase 3
EC=3.5.2.6

Alternative name(s):
Beta-lactamase III

Gene names

Name:

blaZ

Organism

Bacillus cereus

Taxonomic identifier

1396 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Cell membrane; Lipid-anchor Probable.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Hydrolase
PTM	Lipoprotein Palmitate
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29
	Chain	30 – 316	287	Beta-lactamase 3		PRO_0000016968
Regions
	Region	257 – 259	3	Substrate binding By similarity
Sites
	Active site	95	1	Acyl-ester intermediate By similarity
	Active site	191	1	Proton acceptor By similarity
Amino acid modifications
	Lipidation	30	1	N-palmitoyl cysteine Probable
	Lipidation	30	1	S-diacylglycerol cysteine

Sequences

	Sequence		Length	Mass (Da)
	P06548 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 27A082AB9CCBC9DF	FASTA	316	35,141
	10 20 30 40 50 60 MFVLNKFFTN SHYKKIVPVV LLSCATLIGC SNSNTQSESN KQTNQTNQVK QENKRNHAFA 70 80 90 100 110 120 KLEKEYNAKL GIYALDTSTN QTVAYHADDR FAFASTSKSL AVGALLRQNS IEALDERITY 130 140 150 160 170 180 TRKDLSNYNP ITEKHVDTGM TLKELADASV RYSDSTAHNL ILKKLGGPSA FEKILREMGD 190 200 210 220 230 240 TVTNSERFEP ELNEVNPGET HDTSTPKAIA KTLQSFTLGT VLPSEKRELL VDWMKRNTTG 250 260 270 280 290 300 DKLIRAGVPK GWEVADKTGA GSYGTRNDIA IIWPPNKKPI VLSILSNHDK EDAEYDDTLI 310 ADATKIVLET LKVTNK

References

[1]	"Cloning and sequencing of the blaZ gene encoding beta-lactamase III, a lipoprotein of Bacillus cereus 569/H." Hussain M., Pastor F.I.J., Lampen J.O. J. Bacteriol. 169:579-586(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 135-146. Strain: 569/H / NCTC 9945.
[2]	"Beta-lactamase III of Bacillus cereus 569: membrane lipoprotein and secreted protein." Nielsen J.B., Lampen J.O. Biochemistry 22:4652-4656(1983) [PubMed] [Europe PMC] [Abstract] Cited for: DIACYLGLYCEROL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15195 Genomic DNA. Translation: AAA22274.1.
PIR	PNBSLC. A27755.
3D structure databases
ProteinModelPortal	P06548.
SMR	P06548. Positions 59-311.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA3_BACCE

Accession

Primary (citable) accession number: P06548

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program