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P06548

- BLA3_BACCE

UniProt

P06548 - BLA3_BACCE

Protein

Beta-lactamase 3

Gene

blaZ

Organism
Bacillus cereus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    • Comment

    Functioni

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei95 – 951Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei191 – 1911Proton acceptorBy similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase 3 (EC:3.5.2.6)
    Alternative name(s):
    Beta-lactamase III
    Gene namesi
    Name:blaZ
    OrganismiBacillus cereus
    Taxonomic identifieri1396 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Add
    BLAST
    Chaini30 – 316287Beta-lactamase 3PRO_0000016968Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi30 – 301N-palmitoyl cysteine1 Publication
    Lipidationi30 – 301S-diacylglycerol cysteine1 PublicationPROSITE-ProRule annotation

    Keywords - PTMi

    Lipoprotein, Palmitate

    Structurei

    3D structure databases

    ProteinModelPortaliP06548.
    SMRiP06548. Positions 59-311.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni257 – 2593Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06548-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFVLNKFFTN SHYKKIVPVV LLSCATLIGC SNSNTQSESN KQTNQTNQVK    50
    QENKRNHAFA KLEKEYNAKL GIYALDTSTN QTVAYHADDR FAFASTSKSL 100
    AVGALLRQNS IEALDERITY TRKDLSNYNP ITEKHVDTGM TLKELADASV 150
    RYSDSTAHNL ILKKLGGPSA FEKILREMGD TVTNSERFEP ELNEVNPGET 200
    HDTSTPKAIA KTLQSFTLGT VLPSEKRELL VDWMKRNTTG DKLIRAGVPK 250
    GWEVADKTGA GSYGTRNDIA IIWPPNKKPI VLSILSNHDK EDAEYDDTLI 300
    ADATKIVLET LKVTNK 316
    Length:316
    Mass (Da):35,141
    Last modified:January 1, 1988 - v1
    Checksum:i27A082AB9CCBC9DF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15195 Genomic DNA. Translation: AAA22274.1.
    PIRiA27755. PNBSLC.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15195 Genomic DNA. Translation: AAA22274.1 .
    PIRi A27755. PNBSLC.

    3D structure databases

    ProteinModelPortali P06548.
    SMRi P06548. Positions 59-311.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    Pfami PF00144. Beta-lactamase. 1 hit.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the blaZ gene encoding beta-lactamase III, a lipoprotein of Bacillus cereus 569/H."
      Hussain M., Pastor F.I.J., Lampen J.O.
      J. Bacteriol. 169:579-586(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 135-146.
      Strain: 569/H / NCTC 9945.
    2. "Beta-lactamase III of Bacillus cereus 569: membrane lipoprotein and secreted protein."
      Nielsen J.B., Lampen J.O.
      Biochemistry 22:4652-4656(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: DIACYLGLYCEROL AT CYS-30, PALMITOYLATION AT CYS-30.

    Entry informationi

    Entry nameiBLA3_BACCE
    AccessioniPrimary (citable) accession number: P06548
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3