Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-lactamase 3

Gene

blaZ

Organism
Bacillus cereus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei191 – 1911Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase 3 (EC:3.5.2.6)
Alternative name(s):
Beta-lactamase III
Gene namesi
Name:blaZ
OrganismiBacillus cereus
Taxonomic identifieri1396 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Add
BLAST
Chaini30 – 316287Beta-lactamase 3PRO_0000016968Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi30 – 301N-palmitoyl cysteine1 Publication
Lipidationi30 – 301S-diacylglycerol cysteinePROSITE-ProRule annotation1 Publication

Keywords - PTMi

Lipoprotein, Palmitate

Structurei

3D structure databases

ProteinModelPortaliP06548.
SMRiP06548. Positions 59-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni257 – 2593Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVLNKFFTN SHYKKIVPVV LLSCATLIGC SNSNTQSESN KQTNQTNQVK
60 70 80 90 100
QENKRNHAFA KLEKEYNAKL GIYALDTSTN QTVAYHADDR FAFASTSKSL
110 120 130 140 150
AVGALLRQNS IEALDERITY TRKDLSNYNP ITEKHVDTGM TLKELADASV
160 170 180 190 200
RYSDSTAHNL ILKKLGGPSA FEKILREMGD TVTNSERFEP ELNEVNPGET
210 220 230 240 250
HDTSTPKAIA KTLQSFTLGT VLPSEKRELL VDWMKRNTTG DKLIRAGVPK
260 270 280 290 300
GWEVADKTGA GSYGTRNDIA IIWPPNKKPI VLSILSNHDK EDAEYDDTLI
310
ADATKIVLET LKVTNK
Length:316
Mass (Da):35,141
Last modified:January 1, 1988 - v1
Checksum:i27A082AB9CCBC9DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15195 Genomic DNA. Translation: AAA22274.1.
PIRiA27755. PNBSLC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15195 Genomic DNA. Translation: AAA22274.1.
PIRiA27755. PNBSLC.

3D structure databases

ProteinModelPortaliP06548.
SMRiP06548. Positions 59-311.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of the blaZ gene encoding beta-lactamase III, a lipoprotein of Bacillus cereus 569/H."
    Hussain M., Pastor F.I.J., Lampen J.O.
    J. Bacteriol. 169:579-586(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 135-146.
    Strain: 569/H / NCTC 9945.
  2. "Beta-lactamase III of Bacillus cereus 569: membrane lipoprotein and secreted protein."
    Nielsen J.B., Lampen J.O.
    Biochemistry 22:4652-4656(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: DIACYLGLYCEROL AT CYS-30, PALMITOYLATION AT CYS-30.

Entry informationi

Entry nameiBLA3_BACCE
AccessioniPrimary (citable) accession number: P06548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: January 7, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.