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P06548 (BLA3_BACCE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase 3

EC=3.5.2.6
Alternative name(s):
Beta-lactamase III
Gene names
Name:blaZ
OrganismBacillus cereus
Taxonomic identifier1396 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Subcellular location

Cell membrane; Lipid-anchor Probable.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentCell membrane
Membrane
   DomainSignal
   Molecular functionHydrolase
   PTMLipoprotein
Palmitate
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929
Chain30 – 316287Beta-lactamase 3
PRO_0000016968

Regions

Region257 – 2593Substrate binding By similarity

Sites

Active site951Acyl-ester intermediate By similarity
Active site1911Proton acceptor By similarity

Amino acid modifications

Lipidation301N-palmitoyl cysteine Probable
Lipidation301S-diacylglycerol cysteine

Sequences

Sequence LengthMass (Da)Tools
P06548 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 27A082AB9CCBC9DF

FASTA31635,141
        10         20         30         40         50         60 
MFVLNKFFTN SHYKKIVPVV LLSCATLIGC SNSNTQSESN KQTNQTNQVK QENKRNHAFA 

        70         80         90        100        110        120 
KLEKEYNAKL GIYALDTSTN QTVAYHADDR FAFASTSKSL AVGALLRQNS IEALDERITY 

       130        140        150        160        170        180 
TRKDLSNYNP ITEKHVDTGM TLKELADASV RYSDSTAHNL ILKKLGGPSA FEKILREMGD 

       190        200        210        220        230        240 
TVTNSERFEP ELNEVNPGET HDTSTPKAIA KTLQSFTLGT VLPSEKRELL VDWMKRNTTG 

       250        260        270        280        290        300 
DKLIRAGVPK GWEVADKTGA GSYGTRNDIA IIWPPNKKPI VLSILSNHDK EDAEYDDTLI 

       310 
ADATKIVLET LKVTNK 

« Hide

References

[1]"Cloning and sequencing of the blaZ gene encoding beta-lactamase III, a lipoprotein of Bacillus cereus 569/H."
Hussain M., Pastor F.I.J., Lampen J.O.
J. Bacteriol. 169:579-586(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 135-146.
Strain: 569/H / NCTC 9945.
[2]"Beta-lactamase III of Bacillus cereus 569: membrane lipoprotein and secreted protein."
Nielsen J.B., Lampen J.O.
Biochemistry 22:4652-4656(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: DIACYLGLYCEROL AT CYS-30, PALMITOYLATION AT CYS-30.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M15195 Genomic DNA. Translation: AAA22274.1.
PIRPNBSLC. A27755.

3D structure databases

ProteinModelPortalP06548.
SMRP06548. Positions 59-311.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLA3_BACCE
AccessionPrimary (citable) accession number: P06548
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families