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Protein

Stage 0 sporulation protein A

Gene

spo0A

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process. Repressor of abrB, activator of the spoIIa operon. Binds the DNA sequence 5'-TGNCGAA-3' (0A box).

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10CalciumBy similarity1
Metal bindingi11CalciumBy similarity1
Metal bindingi56CalciumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi199 – 218H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • asymmetric cell division Source: UniProtKB
  • barrier septum assembly Source: UniProtKB
  • detection of stimulus Source: InterPro
  • multicellular pellicle formation Source: CACAO
  • phosphorelay signal transduction system Source: UniProtKB-KW
  • positive regulation of sporulation resulting in formation of a cellular spore Source: UniProtKB
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Sporulation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

Calcium, DNA-binding, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU24220-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Stage 0 sporulation protein A
Alternative name(s):
Stage 0 sporulation protein C
Stage 0 sporulation protein G
Gene namesi
Name:spo0A
Synonyms:spo0C, spo0G
Ordered Locus Names:BSU24220
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12N → S in SOF-1 mutant. 1 Publication1
Mutagenesisi60P → S in COI-1 mutant. 1 Publication1
Mutagenesisi87A → V in COI-2 and COI-12 mutants. 1 Publication1
Mutagenesisi90Q → K in COI-15 mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000812341 – 267Stage 0 sporulation protein AAdd BLAST267

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei564-aspartylphosphatePROSITE-ProRule annotation1

Post-translational modificationi

Phosphorylated by KinA and KinB.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP06534.
PRIDEiP06534.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013286.

Structurei

Secondary structure

1267
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi153 – 164Combined sources12
Helixi171 – 185Combined sources15
Helixi187 – 191Combined sources5
Turni193 – 196Combined sources4
Helixi197 – 204Combined sources8
Helixi209 – 225Combined sources17
Helixi231 – 235Combined sources5
Helixi249 – 262Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LQ1X-ray2.30A/B/C/D148-267[»]
ProteinModelPortaliP06534.
SMRiP06534.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06534.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 123Response regulatoryPROSITE-ProRule annotationAdd BLAST119

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107QQR. Bacteria.
COG0784. LUCA.
HOGENOMiHOG000057639.
KOiK07699.
OMAiTKELYPM.
PhylomeDBiP06534.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR014879. Spo0A_C.
IPR012052. Spore_0_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF08769. Spo0A_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002937. Res_reg_Spo0A. 1 hit.
ProDomiPD010908. Spo0A_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
TIGRFAMsiTIGR02875. spore_0_A. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKIKVCVAD DNRELVSLLS EYIEGQEDME VIGVAYNGQE CLSLFKEKDP
60 70 80 90 100
DVLVLDIIMP HLDGLAVLER LRESDLKKQP NVIMLTAFGQ EDVTKKAVDL
110 120 130 140 150
GASYFILKPF DMENLVGHIR QVSGNASSVT HRAPSSQSSI IRSSQPEPKK
160 170 180 190 200
KNLDASITSI IHEIGVPAHI KGYLYLREAI SMVYNDIELL GSITKVLYPD
210 220 230 240 250
IAKKFNTTAS RVERAIRHAI EVAWSRGNID SISSLFGYTV SMTKAKPTNS
260
EFIAMVADKL RLEHKAS
Length:267
Mass (Da):29,691
Last modified:January 1, 1988 - v1
Checksum:i2F4943A5A5061DD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10082 Genomic DNA. Translation: AAA22786.1.
D84432 Genomic DNA. Translation: BAA12581.1.
AL009126 Genomic DNA. Translation: CAB14353.1.
U68235 Genomic DNA. Translation: AAC44872.1.
M23656 Genomic DNA. Translation: AAA22842.1.
PIRiA94036. SZBS0A.
RefSeqiNP_390302.1. NC_000964.3.
WP_003226427.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14353; CAB14353; BSU24220.
GeneIDi11240094.
938655.
KEGGibsu:BSU24220.
PATRICi18976676. VBIBacSub10457_2528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10082 Genomic DNA. Translation: AAA22786.1.
D84432 Genomic DNA. Translation: BAA12581.1.
AL009126 Genomic DNA. Translation: CAB14353.1.
U68235 Genomic DNA. Translation: AAC44872.1.
M23656 Genomic DNA. Translation: AAA22842.1.
PIRiA94036. SZBS0A.
RefSeqiNP_390302.1. NC_000964.3.
WP_003226427.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LQ1X-ray2.30A/B/C/D148-267[»]
ProteinModelPortaliP06534.
SMRiP06534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013286.

Proteomic databases

PaxDbiP06534.
PRIDEiP06534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14353; CAB14353; BSU24220.
GeneIDi11240094.
938655.
KEGGibsu:BSU24220.
PATRICi18976676. VBIBacSub10457_2528.

Phylogenomic databases

eggNOGiENOG4107QQR. Bacteria.
COG0784. LUCA.
HOGENOMiHOG000057639.
KOiK07699.
OMAiTKELYPM.
PhylomeDBiP06534.

Enzyme and pathway databases

BioCyciBSUB:BSU24220-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06534.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR014879. Spo0A_C.
IPR012052. Spore_0_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF08769. Spo0A_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002937. Res_reg_Spo0A. 1 hit.
ProDomiPD010908. Spo0A_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
TIGRFAMsiTIGR02875. spore_0_A. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSP0A_BACSU
AccessioniPrimary (citable) accession number: P06534
Secondary accession number(s): P70990
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Stage 0 mutants lack the ability to form the asymmetric septum characteristic of the initiation of the sporulation process.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.