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Protein

Stage 0 sporulation protein A

Gene

spo0A

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process. Repressor of abrB, activator of the spoIIa operon. Binds the DNA sequence 5'-TGNCGAA-3' (0A box).

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101CalciumBy similarity
Metal bindingi11 – 111CalciumBy similarity
Metal bindingi56 – 561CalciumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi199 – 21820H-T-H motifSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • asymmetric cell division Source: UniProtKB
  • barrier septum assembly Source: UniProtKB
  • detection of stimulus Source: InterPro
  • multicellular pellicle formation Source: CACAO
  • phosphorelay signal transduction system Source: UniProtKB-KW
  • positive regulation of sporulation resulting in formation of a cellular spore Source: UniProtKB
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Sporulation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

Calcium, DNA-binding, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU24220-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Stage 0 sporulation protein A
Alternative name(s):
Stage 0 sporulation protein C
Stage 0 sporulation protein G
Gene namesi
Name:spo0A
Synonyms:spo0C, spo0G
Ordered Locus Names:BSU24220
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi12 – 121N → S in SOF-1 mutant. 1 Publication
Mutagenesisi60 – 601P → S in COI-1 mutant. 1 Publication
Mutagenesisi87 – 871A → V in COI-2 and COI-12 mutants. 1 Publication
Mutagenesisi90 – 901Q → K in COI-15 mutant. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Stage 0 sporulation protein APRO_0000081234Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 5614-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by KinA and KinB.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP06534.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013286.

Structurei

Secondary structure

1
267
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi153 – 16412Combined sources
Helixi171 – 18515Combined sources
Helixi187 – 1915Combined sources
Turni193 – 1964Combined sources
Helixi197 – 2048Combined sources
Helixi209 – 22517Combined sources
Helixi231 – 2355Combined sources
Helixi249 – 26214Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LQ1X-ray2.30A/B/C/D148-267[»]
ProteinModelPortaliP06534.
SMRiP06534. Positions 4-124, 151-266.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06534.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 123119Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107QQR. Bacteria.
COG0784. LUCA.
HOGENOMiHOG000057639.
KOiK07699.
OMAiTKELYPM.
PhylomeDBiP06534.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR014879. Spo0A_C.
IPR012052. Spore_0_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF08769. Spo0A_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002937. Res_reg_Spo0A. 1 hit.
ProDomiPD010908. Spo0A_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
TIGRFAMsiTIGR02875. spore_0_A. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKIKVCVAD DNRELVSLLS EYIEGQEDME VIGVAYNGQE CLSLFKEKDP
60 70 80 90 100
DVLVLDIIMP HLDGLAVLER LRESDLKKQP NVIMLTAFGQ EDVTKKAVDL
110 120 130 140 150
GASYFILKPF DMENLVGHIR QVSGNASSVT HRAPSSQSSI IRSSQPEPKK
160 170 180 190 200
KNLDASITSI IHEIGVPAHI KGYLYLREAI SMVYNDIELL GSITKVLYPD
210 220 230 240 250
IAKKFNTTAS RVERAIRHAI EVAWSRGNID SISSLFGYTV SMTKAKPTNS
260
EFIAMVADKL RLEHKAS
Length:267
Mass (Da):29,691
Last modified:January 1, 1988 - v1
Checksum:i2F4943A5A5061DD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10082 Genomic DNA. Translation: AAA22786.1.
D84432 Genomic DNA. Translation: BAA12581.1.
AL009126 Genomic DNA. Translation: CAB14353.1.
U68235 Genomic DNA. Translation: AAC44872.1.
M23656 Genomic DNA. Translation: AAA22842.1.
PIRiA94036. SZBS0A.
RefSeqiNP_390302.1. NC_000964.3.
WP_003226427.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14353; CAB14353; BSU24220.
GeneIDi11240094.
938655.
KEGGibsu:BSU24220.
PATRICi18976676. VBIBacSub10457_2528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10082 Genomic DNA. Translation: AAA22786.1.
D84432 Genomic DNA. Translation: BAA12581.1.
AL009126 Genomic DNA. Translation: CAB14353.1.
U68235 Genomic DNA. Translation: AAC44872.1.
M23656 Genomic DNA. Translation: AAA22842.1.
PIRiA94036. SZBS0A.
RefSeqiNP_390302.1. NC_000964.3.
WP_003226427.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LQ1X-ray2.30A/B/C/D148-267[»]
ProteinModelPortaliP06534.
SMRiP06534. Positions 4-124, 151-266.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013286.

Proteomic databases

PaxDbiP06534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14353; CAB14353; BSU24220.
GeneIDi11240094.
938655.
KEGGibsu:BSU24220.
PATRICi18976676. VBIBacSub10457_2528.

Phylogenomic databases

eggNOGiENOG4107QQR. Bacteria.
COG0784. LUCA.
HOGENOMiHOG000057639.
KOiK07699.
OMAiTKELYPM.
PhylomeDBiP06534.

Enzyme and pathway databases

BioCyciBSUB:BSU24220-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06534.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR014879. Spo0A_C.
IPR012052. Spore_0_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF08769. Spo0A_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002937. Res_reg_Spo0A. 1 hit.
ProDomiPD010908. Spo0A_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
TIGRFAMsiTIGR02875. spore_0_A. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSP0A_BACSU
AccessioniPrimary (citable) accession number: P06534
Secondary accession number(s): P70990
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 7, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Stage 0 mutants lack the ability to form the asymmetric septum characteristic of the initiation of the sporulation process.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.