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Protein

Stage 0 sporulation protein A

Gene

spo0A

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process. Repressor of abrB, activator of the spoIIa operon. Binds the DNA sequence 5'-TGNCGAA-3' (0A box).

Miscellaneous

Stage 0 mutants lack the ability to form the asymmetric septum characteristic of the initiation of the sporulation process.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10CalciumBy similarity1
Metal bindingi11CalciumBy similarity1
Metal bindingi56CalciumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi199 – 218H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • asymmetric cell division Source: UniProtKB
  • cell septum assembly Source: UniProtKB
  • detection of stimulus Source: InterPro
  • phosphorelay signal transduction system Source: UniProtKB-KW
  • positive regulation of sporulation resulting in formation of a cellular spore Source: UniProtKB
  • single-species surface biofilm formation Source: CACAO
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processSporulation, Transcription, Transcription regulation, Two-component regulatory system
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU24220-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Stage 0 sporulation protein A
Alternative name(s):
Stage 0 sporulation protein C
Stage 0 sporulation protein G
Gene namesi
Name:spo0A
Synonyms:spo0C, spo0G
Ordered Locus Names:BSU24220
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12N → S in SOF-1 mutant. 1 Publication1
Mutagenesisi60P → S in COI-1 mutant. 1 Publication1
Mutagenesisi87A → V in COI-2 and COI-12 mutants. 1 Publication1
Mutagenesisi90Q → K in COI-15 mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000812341 – 267Stage 0 sporulation protein AAdd BLAST267

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei564-aspartylphosphatePROSITE-ProRule annotation1

Post-translational modificationi

Phosphorylated by KinA and KinB.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP06534
PRIDEiP06534

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013286

Structurei

Secondary structure

1267
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi153 – 164Combined sources12
Helixi171 – 185Combined sources15
Helixi187 – 191Combined sources5
Turni193 – 196Combined sources4
Helixi197 – 204Combined sources8
Helixi209 – 225Combined sources17
Helixi231 – 235Combined sources5
Helixi249 – 262Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LQ1X-ray2.30A/B/C/D148-267[»]
ProteinModelPortaliP06534
SMRiP06534
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06534

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 123Response regulatoryPROSITE-ProRule annotationAdd BLAST119

Phylogenomic databases

eggNOGiENOG4107QQR Bacteria
COG0784 LUCA
HOGENOMiHOG000057639
KOiK07699
OMAiTKELYPM
PhylomeDBiP06534

Family and domain databases

CDDicd00156 REC, 1 hit
Gene3Di1.10.10.101 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR016032 Sig_transdc_resp-reg_C-effctor
IPR001789 Sig_transdc_resp-reg_receiver
IPR014879 Spo0A_C
IPR012052 Spore_0_A
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00072 Response_reg, 1 hit
PF08769 Spo0A_C, 1 hit
PIRSFiPIRSF002937 Res_reg_Spo0A, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD010908 Spo0A_C, 1 hit
SMARTiView protein in SMART
SM00448 REC, 1 hit
SUPFAMiSSF46894 SSF46894, 1 hit
SSF52172 SSF52172, 1 hit
TIGRFAMsiTIGR02875 spore_0_A, 1 hit
PROSITEiView protein in PROSITE
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

P06534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKIKVCVAD DNRELVSLLS EYIEGQEDME VIGVAYNGQE CLSLFKEKDP
60 70 80 90 100
DVLVLDIIMP HLDGLAVLER LRESDLKKQP NVIMLTAFGQ EDVTKKAVDL
110 120 130 140 150
GASYFILKPF DMENLVGHIR QVSGNASSVT HRAPSSQSSI IRSSQPEPKK
160 170 180 190 200
KNLDASITSI IHEIGVPAHI KGYLYLREAI SMVYNDIELL GSITKVLYPD
210 220 230 240 250
IAKKFNTTAS RVERAIRHAI EVAWSRGNID SISSLFGYTV SMTKAKPTNS
260
EFIAMVADKL RLEHKAS
Length:267
Mass (Da):29,691
Last modified:January 1, 1988 - v1
Checksum:i2F4943A5A5061DD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10082 Genomic DNA Translation: AAA22786.1
D84432 Genomic DNA Translation: BAA12581.1
AL009126 Genomic DNA Translation: CAB14353.1
U68235 Genomic DNA Translation: AAC44872.1
M23656 Genomic DNA Translation: AAA22842.1
PIRiA94036 SZBS0A
RefSeqiNP_390302.1, NC_000964.3
WP_003226427.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14353; CAB14353; BSU24220
GeneIDi938655
KEGGibsu:BSU24220
PATRICifig|224308.179.peg.2639

Similar proteinsi

Entry informationi

Entry nameiSP0A_BACSU
AccessioniPrimary (citable) accession number: P06534
Secondary accession number(s): P70990
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: March 28, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome