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Protein

HTH-type transcriptional regulator SinR

Gene

sinR

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Negative as well as positive regulator of alternate developmental processes that are induced at the end of vegetative growth in response to nutrient depletion. Binds to the alkaline protease (aprE) gene at two sites. Also acts as a repressor of the key sporulation gene spo0A. Negatively regulates transcription of the eps operon, which is responsible for the biosynthesis of an exopolysaccharide involved in biofilm formation; therefore it could govern the transition between a state in which bacteria swim or swarm and a state in which bacteria assemble into multicellular communities. Acts with Hpr as a corepressor of epr expression. Also negatively regulates transcription of the lutABC operon, which is required for lactate utilization. Repressor activity is regulated by SinI.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi17 – 36H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Sporulation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU24610-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator SinR
Gene namesi
Name:sinR
Synonyms:flaD, sin
Ordered Locus Names:BSU24610
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001497381 – 111HTH-type transcriptional regulator SinRAdd BLAST111

Proteomic databases

PaxDbiP06533.

Interactioni

Subunit structurei

Homotetramer in the absence of SinI. Heterodimer with SinI. Interaction with SinI disrupts the SinR tetramer and its repressor activity. Interacts with hpr.3 Publications

Protein-protein interaction databases

DIPiDIP-6102N.
IntActiP06533. 2 interactors.
MINTiMINT-1540382.
STRINGi224308.Bsubs1_010100013486.

Structurei

Secondary structure

1111
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Helixi17 – 24Combined sources8
Helixi28 – 35Combined sources8
Helixi44 – 54Combined sources11
Helixi58 – 62Combined sources5
Helixi76 – 87Combined sources12
Helixi92 – 107Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B0NX-ray1.90A1-111[»]
2YALX-ray2.27A/B75-111[»]
3QQ6X-ray1.90A/B1-69[»]
3ZKCX-ray3.00A/B1-111[»]
ProteinModelPortaliP06533.
SMRiP06533.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06533.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 61HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST56
Domaini65 – 103SinPROSITE-ProRule annotationAdd BLAST39

Sequence similaritiesi

Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 Sin domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108IMY. Bacteria.
COG1396. LUCA.
HOGENOMiHOG000225390.
InParanoidiP06533.
KOiK19449.
OMAiYQKWRKA.
PhylomeDBiP06533.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
1.10.287.270. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR010981. SinR/SinI_dimer_dom.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF08671. SinI. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47406. SSF47406. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
PS51500. SIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06533-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGQRIKQYR KEKGYSLSEL AEKAGVAKSY LSSIERNLQT NPSIQFLEKV
60 70 80 90 100
SAVLDVSVHT LLDEKHETEY DGQLDSEWEK LVRDAMTSGV SKKQFREFLD
110
YQKWRKSQKE E
Length:111
Mass (Da):12,989
Last modified:January 1, 1988 - v1
Checksum:iB6FC6463FF4B52C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14112 Genomic DNA. Translation: AAA22757.1.
D84432 Genomic DNA. Translation: BAA12542.1.
AL009126 Genomic DNA. Translation: CAB14392.1.
PIRiB25159.
RefSeqiNP_390341.1. NC_000964.3.
WP_003226345.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14392; CAB14392; BSU24610.
GeneIDi938544.
KEGGibsu:BSU24610.
PATRICi18976756. VBIBacSub10457_2568.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14112 Genomic DNA. Translation: AAA22757.1.
D84432 Genomic DNA. Translation: BAA12542.1.
AL009126 Genomic DNA. Translation: CAB14392.1.
PIRiB25159.
RefSeqiNP_390341.1. NC_000964.3.
WP_003226345.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B0NX-ray1.90A1-111[»]
2YALX-ray2.27A/B75-111[»]
3QQ6X-ray1.90A/B1-69[»]
3ZKCX-ray3.00A/B1-111[»]
ProteinModelPortaliP06533.
SMRiP06533.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6102N.
IntActiP06533. 2 interactors.
MINTiMINT-1540382.
STRINGi224308.Bsubs1_010100013486.

Proteomic databases

PaxDbiP06533.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14392; CAB14392; BSU24610.
GeneIDi938544.
KEGGibsu:BSU24610.
PATRICi18976756. VBIBacSub10457_2568.

Phylogenomic databases

eggNOGiENOG4108IMY. Bacteria.
COG1396. LUCA.
HOGENOMiHOG000225390.
InParanoidiP06533.
KOiK19449.
OMAiYQKWRKA.
PhylomeDBiP06533.

Enzyme and pathway databases

BioCyciBSUB:BSU24610-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06533.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
1.10.287.270. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR010981. SinR/SinI_dimer_dom.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF08671. SinI. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47406. SSF47406. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
PS51500. SIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSINR_BACSU
AccessioniPrimary (citable) accession number: P06533
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.