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P06531

- TRPG_EMENI

UniProt

P06531 - TRPG_EMENI

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Protein

Multifunctional tryptophan biosynthesis protein

Gene

trpC

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041For GATase activityBy similarity
Active sitei199 – 1991For GATase activityBy similarity
Active sitei201 – 2011For GATase activityBy similarity

GO - Molecular functioni

  1. anthranilate synthase activity Source: UniProtKB-EC
  2. glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: ASPGD
  3. indole-3-glycerol-phosphate synthase activity Source: ASPGD
  4. phosphoribosylanthranilate isomerase activity Source: ASPGD
  5. transferase activity Source: UniProtKB-KW

GO - Biological processi

  1. cleistothecium development Source: ASPGD
  2. glutamine metabolic process Source: UniProtKB-KW
  3. oxidation-reduction process Source: GOC
  4. para-aminobenzoic acid biosynthetic process Source: ASPGD
  5. tryptophan biosynthetic process Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Isomerase, Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Protein family/group databases

MEROPSiC26.959.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trpC
ORF Names:AN0648
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560: Chromosome VIII

Subcellular locationi

GO - Cellular componenti

  1. anthranilate synthase complex Source: ASPGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 768768Multifunctional tryptophan biosynthesis proteinPRO_0000056859Add
BLAST

Expressioni

Inductioni

By tryptophan starvation.

Structurei

3D structure databases

ProteinModelPortaliP06531.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 225201Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni252 – 516265Indole-3-glycerol phosphate synthaseAdd
BLAST
Regioni532 – 768237N-(5'-phosphoribosyl)anthranilate isomeraseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0134.
HOGENOMiHOG000280459.
InParanoidiP06531.
OrthoDBiEOG78WM1Q.

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06531-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADTALVDHS PHHPTKAPRL ETASNVILID NYDSFTWNVY QYLVLEGATV
60 70 80 90 100
TVIRNDEISL EELIAKKPTQ LVVSPGPGHP KSDAGISNAA IQYFAGKIPI
110 120 130 140 150
FGVCMGQQCI IHSFGGKVDV TGEILHGKTS VLKHDGRGAY EGLPPSVIIT
160 170 180 190 200
RYHSLAGTHS TIPECLEVSS FAQLGEDADK TVIMGVRHKQ FAVEGVQFHP
210 220 230 240 250
ESILTEHGQT MFRNFLKLTA GTWEGNGKDV AQGGNFTAAA PNPPKATKKV
260 270 280 290 300
SILEKIYDHR RAAVAKQKTI PSQRPSDLQA AYELSVAPPQ ISFPDRLRQS
310 320 330 340 350
AYPLSLMAEI KRASPSKGLI AEHACAPAQA RQYAKAGASV ISVLTEPEWF
360 370 380 390 400
KGSIDDLRAV RASLEGLTNR PAILRKEFIF DEYQILEARL AGADTVLLIV
410 420 430 440 450
KMLDTELLTR LYLYSQSLGM EPLVEVNTPD EMKIAVDLGA QVIGVNNRDL
460 470 480 490 500
TSFEVDLGTT SRLMDQVPES TIVCALSGIS GPKDVEAYKK DGVKAILVGE
510 520 530 540 550
ALMRAPDTAA FVAELLGGQS KKLPLQSRNS PLVKICGTRT EEGARAAIEA
560 570 580 590 600
GADLIGIILV EGRKRTVPDD VALQISKVVK STPRPTPYPT EVPQGDTDAT
610 620 630 640 650
SVDYFDHSAT TLRHPTRALL VGVFLNQPLS YVLAQQQKLG LDVVQLHGSE
660 670 680 690 700
PLEWSRLIPV PVIRKFGLDE FGIARRAYHT LPLLDSGAGG SGELLDQMRV
710 720 730 740 750
KQILKSDDGL RVILAGGLDP LNVTEIIKQL DESGYKIVGV DVSSGVETNG
760
VQDLDKIRSF VQAAKSAF
Length:768
Mass (Da):83,127
Last modified:October 19, 2011 - v3
Checksum:iFEFE1374DF86151D
GO

Sequence cautioni

The sequence CBF89063.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence EAA65153.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1111I → H in CAA26232. (PubMed:3158796)Curated
Sequence conflicti249 – 2491K → Q in CAA26232. (PubMed:3158796)Curated
Sequence conflicti396 – 3983VLL → SYV in CAA26232. (PubMed:3158796)Curated
Sequence conflicti396 – 3983VLL → SYV(PubMed:6324178)Curated
Sequence conflicti410 – 4156RLYLYS → QTLSLF in CAA26232. (PubMed:3158796)Curated
Sequence conflicti410 – 4101R → Q(PubMed:6324178)Curated
Sequence conflicti473 – 48210VCALSGISGP → RLCTMRYFWT in CAA26232. (PubMed:3158796)Curated
Sequence conflicti681 – 6811L → V in CAA26232. (PubMed:3158796)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02390 Genomic DNA. Translation: CAA26232.1.
AACD01000009 Genomic DNA. Translation: EAA65153.1. Sequence problems.
U24705 Genomic DNA. Translation: AAB60292.1.
BN001308 Genomic DNA. Translation: CBF89063.1. Different initiation.
PIRiS04518.
S07305.
RefSeqiXP_658252.1. XM_653160.1.

Genome annotation databases

EnsemblFungiiCADANIAT00002028; CADANIAP00002028; CADANIAG00002028.
GeneIDi2876427.
KEGGiani:AN0648.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02390 Genomic DNA. Translation: CAA26232.1 .
AACD01000009 Genomic DNA. Translation: EAA65153.1 . Sequence problems.
U24705 Genomic DNA. Translation: AAB60292.1 .
BN001308 Genomic DNA. Translation: CBF89063.1 . Different initiation.
PIRi S04518.
S07305.
RefSeqi XP_658252.1. XM_653160.1.

3D structure databases

ProteinModelPortali P06531.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C26.959.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANIAT00002028 ; CADANIAP00002028 ; CADANIAG00002028 .
GeneIDi 2876427.
KEGGi ani:AN0648.2.

Phylogenomic databases

eggNOGi COG0134.
HOGENOMi HOG000280459.
InParanoidi P06531.
OrthoDBi EOG78WM1Q.

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00040 .
UPA00035 ; UER00042 .
UPA00035 ; UER00043 .

Family and domain databases

Gene3Di 3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPi MF_00135. PRAI.
InterProi IPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view ]
Pfami PF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view ]
PIRSFi PIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMi SSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsi TIGR00566. trpG_papA. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Aspergillus niger trpC gene: structural relationship with analogous genes of other organisms."
    Kos T., Kuijvenhoven A., Hessing H.G.M., Pouwels P.H., van den Hondel C.A.M.J.J.
    Curr. Genet. 13:137-144(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Primary structure of the trpC gene from Aspergillus nidulans."
    Mullaney E.J., Hamer J.E., Roberti K.A., Yelton M.M., Timberlake W.E.
    Mol. Gen. Genet. 199:37-45(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 7-768.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  4. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 377-410.
  6. Genser K.F.
    Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 719-768.

Entry informationi

Entry nameiTRPG_EMENI
AccessioniPrimary (citable) accession number: P06531
Secondary accession number(s): C8VS06, Q5BFN2, Q92224
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 19, 2011
Last modified: October 29, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3