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Protein

Multifunctional tryptophan biosynthesis protein

Gene

trpC

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (trpC)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (trpC)
  4. Multifunctional tryptophan biosynthesis protein (trpC)
  5. Tryptophan synthase (AN6231.2), Tryptophan synthase (ANIA_06231)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Nucleophile; for GATase activityBy similarity1
Binding sitei108GlutamineBy similarity1
Active sitei199For GATase activityBy similarity1
Active sitei201For GATase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Isomerase, Lyase, Multifunctional enzyme
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040
UPA00035; UER00042
UPA00035; UER00043

Protein family/group databases

MEROPSiC26.959

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trpC
ORF Names:AN0648
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VIII
  • UP000005890 Componenti: Unassembled WGS sequence

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000568591 – 768Multifunctional tryptophan biosynthesis proteinAdd BLAST768

Proteomic databases

PRIDEiP06531

Expressioni

Inductioni

By tryptophan starvation.

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00002028

Structurei

3D structure databases

ProteinModelPortaliP06531
SMRiP06531
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 225Glutamine amidotransferase type-1Add BLAST201

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 78Glutamine bindingBy similarity3
Regioni154 – 155Glutamine bindingBy similarity2
Regioni252 – 516Indole-3-glycerol phosphate synthaseAdd BLAST265
Regioni532 – 768N-(5'-phosphoribosyl)anthranilate isomeraseAdd BLAST237

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000280459
InParanoidiP06531
OrthoDBiEOG092C1PKC

Family and domain databases

CDDicd00331 IGPS, 1 hit
cd00405 PRAI, 1 hit
Gene3Di3.20.20.70, 3 hits
3.40.50.880, 1 hit
HAMAPiMF_00135 PRAI, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR016302 Anthranilate_synth_II
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR013798 Indole-3-glycerol_P_synth
IPR001468 Indole-3-GlycerolPSynthase_CS
IPR001240 PRAI
IPR011060 RibuloseP-bd_barrel
IPR006221 TrpG/PapA_dom
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF00218 IGPS, 1 hit
PF00697 PRAI, 1 hit
PIRSFiPIRSF001382 TrpG-trpC-trpF, 1 hit
SUPFAMiSSF51366 SSF51366, 3 hits
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00566 trpG_papA, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
PS00614 IGPS, 1 hit

Sequencei

Sequence statusi: Complete.

P06531-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTALVDHS PHHPTKAPRL ETASNVILID NYDSFTWNVY QYLVLEGATV
60 70 80 90 100
TVIRNDEISL EELIAKKPTQ LVVSPGPGHP KSDAGISNAA IQYFAGKIPI
110 120 130 140 150
FGVCMGQQCI IHSFGGKVDV TGEILHGKTS VLKHDGRGAY EGLPPSVIIT
160 170 180 190 200
RYHSLAGTHS TIPECLEVSS FAQLGEDADK TVIMGVRHKQ FAVEGVQFHP
210 220 230 240 250
ESILTEHGQT MFRNFLKLTA GTWEGNGKDV AQGGNFTAAA PNPPKATKKV
260 270 280 290 300
SILEKIYDHR RAAVAKQKTI PSQRPSDLQA AYELSVAPPQ ISFPDRLRQS
310 320 330 340 350
AYPLSLMAEI KRASPSKGLI AEHACAPAQA RQYAKAGASV ISVLTEPEWF
360 370 380 390 400
KGSIDDLRAV RASLEGLTNR PAILRKEFIF DEYQILEARL AGADTVLLIV
410 420 430 440 450
KMLDTELLTR LYLYSQSLGM EPLVEVNTPD EMKIAVDLGA QVIGVNNRDL
460 470 480 490 500
TSFEVDLGTT SRLMDQVPES TIVCALSGIS GPKDVEAYKK DGVKAILVGE
510 520 530 540 550
ALMRAPDTAA FVAELLGGQS KKLPLQSRNS PLVKICGTRT EEGARAAIEA
560 570 580 590 600
GADLIGIILV EGRKRTVPDD VALQISKVVK STPRPTPYPT EVPQGDTDAT
610 620 630 640 650
SVDYFDHSAT TLRHPTRALL VGVFLNQPLS YVLAQQQKLG LDVVQLHGSE
660 670 680 690 700
PLEWSRLIPV PVIRKFGLDE FGIARRAYHT LPLLDSGAGG SGELLDQMRV
710 720 730 740 750
KQILKSDDGL RVILAGGLDP LNVTEIIKQL DESGYKIVGV DVSSGVETNG
760
VQDLDKIRSF VQAAKSAF
Length:768
Mass (Da):83,127
Last modified:October 19, 2011 - v3
Checksum:iFEFE1374DF86151D
GO

Sequence cautioni

The sequence CBF89063 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence EAA65153 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111I → H in CAA26232 (PubMed:3158796).Curated1
Sequence conflicti249K → Q in CAA26232 (PubMed:3158796).Curated1
Sequence conflicti396 – 398VLL → SYV in CAA26232 (PubMed:3158796).Curated3
Sequence conflicti396 – 398VLL → SYV (PubMed:6324178).Curated3
Sequence conflicti410 – 415RLYLYS → QTLSLF in CAA26232 (PubMed:3158796).Curated6
Sequence conflicti410R → Q (PubMed:6324178).Curated1
Sequence conflicti473 – 482VCALSGISGP → RLCTMRYFWT in CAA26232 (PubMed:3158796).Curated10
Sequence conflicti681L → V in CAA26232 (PubMed:3158796).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02390 Genomic DNA Translation: CAA26232.1
AACD01000009 Genomic DNA Translation: EAA65153.1 Sequence problems.
U24705 Genomic DNA Translation: AAB60292.1
BN001308 Genomic DNA Translation: CBF89063.1 Different initiation.
PIRiS04518
S07305
RefSeqiXP_658252.1, XM_653160.1

Genome annotation databases

EnsemblFungiiCADANIAT00002028; CADANIAP00002028; CADANIAG00002028
EAA65153; EAA65153; AN0648.2
GeneIDi2876427
KEGGiani:AN0648.2

Similar proteinsi

Entry informationi

Entry nameiTRPG_EMENI
AccessioniPrimary (citable) accession number: P06531
Secondary accession number(s): C8VS06, Q5BFN2, Q92224
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 19, 2011
Last modified: May 23, 2018
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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