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Protein

Modification methylase BsuRI

Gene

hsdRM

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the BsuRI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei157 – 1571PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3340. M.BsuRI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BsuRI (EC:2.1.1.37)
Short name:
M.BsuRI
Alternative name(s):
Cytosine-specific methyltransferase BsuRI
Gene namesi
Name:hsdRM
Synonyms:hsdM
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 436436Modification methylase BsuRIPRO_0000087864Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP06530.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 409351SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06530-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MTLKIDIKGR GKYKPASDYS IDDVKNVLME KIFEESSRII NSDDDLEIIE
60 70 80 90 100
KVDFRTDKIN VLSLFSGCGG LDLGFELAGL AAVIGEQAAM EAFKDKDRFN
110 120 130 140 150
ELRNKSIFHT IYTNDLFKEA NQTYKTNFPG HVIQHEKDIR QVKYFPKCNL
160 170 180 190 200
ILGGFPCPGF SEAGPRLIDD DRNFLYLHFI RSLIQAQPEI FVAENVKGMM
210 220 230 240 250
TLGKGEVLNQ IIEDFASAGY RVQFKLLNAR DYGVPQLRER VIIEGVRKDI
260 270 280 290 300
SFNYKYPSPT HGEETGLKPF KTLRDSIGDL VTDPGPYFTG SYSSIYMSRN
310 320 330 340 350
RKKSWDEQSF TIQASGRQAP LHPGGLSMKK IGKDKWVFPD GEENHRRLSV
360 370 380 390 400
KEIARVQTFP DWFQFSQGTN SQTSINNRLD KQYKQIGNAV PVLLAKAVAS
410 420 430
PIANWAINYL ESSPNNKIKN RERKLSIRTF LRIKTS
Length:436
Mass (Da):49,635
Last modified:January 1, 1988 - v1
Checksum:i07EE0B2CA9140B19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02988 Genomic DNA. Translation: CAA26731.1.
PIRiB23488. XYBSR1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02988 Genomic DNA. Translation: CAA26731.1.
PIRiB23488. XYBSR1.

3D structure databases

ProteinModelPortaliP06530.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3340. M.BsuRI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the BsuRI restriction-modification system."
    Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J.
    Nucleic Acids Res. 13:6403-6421(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R.

Entry informationi

Entry nameiMTBR_BACIU
AccessioniPrimary (citable) accession number: P06530
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.