SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P06530

- MTBR_BACIU

UniProt

P06530 - MTBR_BACIU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Modification methylase BsuRI
Gene
hsdRM, hsdM
Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the BsuRI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei157 – 1571 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3340. M.BsuRI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BsuRI (EC:2.1.1.37)
Short name:
M.BsuRI
Alternative name(s):
Cytosine-specific methyltransferase BsuRI
Gene namesi
Name:hsdRM
Synonyms:hsdM
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 436436Modification methylase BsuRI
PRO_0000087864Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP06530.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 409351SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06530-1 [UniParc]FASTAAdd to Basket

« Hide

MTLKIDIKGR GKYKPASDYS IDDVKNVLME KIFEESSRII NSDDDLEIIE    50
KVDFRTDKIN VLSLFSGCGG LDLGFELAGL AAVIGEQAAM EAFKDKDRFN 100
ELRNKSIFHT IYTNDLFKEA NQTYKTNFPG HVIQHEKDIR QVKYFPKCNL 150
ILGGFPCPGF SEAGPRLIDD DRNFLYLHFI RSLIQAQPEI FVAENVKGMM 200
TLGKGEVLNQ IIEDFASAGY RVQFKLLNAR DYGVPQLRER VIIEGVRKDI 250
SFNYKYPSPT HGEETGLKPF KTLRDSIGDL VTDPGPYFTG SYSSIYMSRN 300
RKKSWDEQSF TIQASGRQAP LHPGGLSMKK IGKDKWVFPD GEENHRRLSV 350
KEIARVQTFP DWFQFSQGTN SQTSINNRLD KQYKQIGNAV PVLLAKAVAS 400
PIANWAINYL ESSPNNKIKN RERKLSIRTF LRIKTS 436
Length:436
Mass (Da):49,635
Last modified:January 1, 1988 - v1
Checksum:i07EE0B2CA9140B19
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02988 Genomic DNA. Translation: CAA26731.1.
PIRiB23488. XYBSR1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02988 Genomic DNA. Translation: CAA26731.1 .
PIRi B23488. XYBSR1.

3D structure databases

ProteinModelPortali P06530.
ModBasei Search...

Protein family/group databases

REBASEi 3340. M.BsuRI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the BsuRI restriction-modification system."
    Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J.
    Nucleic Acids Res. 13:6403-6421(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R.

Entry informationi

Entry nameiMTBR_BACIU
AccessioniPrimary (citable) accession number: P06530
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi