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Protein

Modification methylase BsuRI

Gene

hsdRM

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the BsuRI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei157PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3340. M.BsuRI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BsuRI (EC:2.1.1.37)
Short name:
M.BsuRI
Alternative name(s):
Cytosine-specific methyltransferase BsuRI
Gene namesi
Name:hsdRM
Synonyms:hsdM
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878641 – 436Modification methylase BsuRIAdd BLAST436

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP06530.
SMRiP06530.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 409SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST351

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR031303. C5_meth_CS.
IPR029063. SAM-dependent_MTases.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.

Sequencei

Sequence statusi: Complete.

P06530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLKIDIKGR GKYKPASDYS IDDVKNVLME KIFEESSRII NSDDDLEIIE
60 70 80 90 100
KVDFRTDKIN VLSLFSGCGG LDLGFELAGL AAVIGEQAAM EAFKDKDRFN
110 120 130 140 150
ELRNKSIFHT IYTNDLFKEA NQTYKTNFPG HVIQHEKDIR QVKYFPKCNL
160 170 180 190 200
ILGGFPCPGF SEAGPRLIDD DRNFLYLHFI RSLIQAQPEI FVAENVKGMM
210 220 230 240 250
TLGKGEVLNQ IIEDFASAGY RVQFKLLNAR DYGVPQLRER VIIEGVRKDI
260 270 280 290 300
SFNYKYPSPT HGEETGLKPF KTLRDSIGDL VTDPGPYFTG SYSSIYMSRN
310 320 330 340 350
RKKSWDEQSF TIQASGRQAP LHPGGLSMKK IGKDKWVFPD GEENHRRLSV
360 370 380 390 400
KEIARVQTFP DWFQFSQGTN SQTSINNRLD KQYKQIGNAV PVLLAKAVAS
410 420 430
PIANWAINYL ESSPNNKIKN RERKLSIRTF LRIKTS
Length:436
Mass (Da):49,635
Last modified:January 1, 1988 - v1
Checksum:i07EE0B2CA9140B19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02988 Genomic DNA. Translation: CAA26731.1.
PIRiB23488. XYBSR1.

Similar proteinsi

Entry informationi

Entry nameiMTBR_BACIU
AccessioniPrimary (citable) accession number: P06530
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 25, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families