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P06530 (MTBR_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BsuRI

Short name=M.BsuRI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase BsuRI
Gene names
Name:hsdRM
Synonyms:hsdM
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the BsuRI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subunit structure

Monomer.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 436436Modification methylase BsuRI
PRO_0000087864

Regions

Domain59 – 409351SAM-dependent MTase C5-type

Sites

Active site1571 By similarity

Sequences

Sequence LengthMass (Da)Tools
P06530 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 07EE0B2CA9140B19

FASTA43649,635
        10         20         30         40         50         60 
MTLKIDIKGR GKYKPASDYS IDDVKNVLME KIFEESSRII NSDDDLEIIE KVDFRTDKIN 

        70         80         90        100        110        120 
VLSLFSGCGG LDLGFELAGL AAVIGEQAAM EAFKDKDRFN ELRNKSIFHT IYTNDLFKEA 

       130        140        150        160        170        180 
NQTYKTNFPG HVIQHEKDIR QVKYFPKCNL ILGGFPCPGF SEAGPRLIDD DRNFLYLHFI 

       190        200        210        220        230        240 
RSLIQAQPEI FVAENVKGMM TLGKGEVLNQ IIEDFASAGY RVQFKLLNAR DYGVPQLRER 

       250        260        270        280        290        300 
VIIEGVRKDI SFNYKYPSPT HGEETGLKPF KTLRDSIGDL VTDPGPYFTG SYSSIYMSRN 

       310        320        330        340        350        360 
RKKSWDEQSF TIQASGRQAP LHPGGLSMKK IGKDKWVFPD GEENHRRLSV KEIARVQTFP 

       370        380        390        400        410        420 
DWFQFSQGTN SQTSINNRLD KQYKQIGNAV PVLLAKAVAS PIANWAINYL ESSPNNKIKN 

       430 
RERKLSIRTF LRIKTS 

« Hide

References

[1]"Nucleotide sequence of the BsuRI restriction-modification system."
Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J.
Nucleic Acids Res. 13:6403-6421(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: R.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02988 Genomic DNA. Translation: CAA26731.1.
PIRXYBSR1. B23488.

3D structure databases

ProteinModelPortalP06530.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3340. M.BsuRI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTBR_BACIU
AccessionPrimary (citable) accession number: P06530
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries