ID   T2BR_BACIU              Reviewed;         576 AA.
AC   P06529;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   03-MAY-2023, entry version 75.
DE   RecName: Full=Type II restriction enzyme BsuRI {ECO:0000303|PubMed:12654995};
DE            Short=R.BsuRI;
DE            EC=3.1.21.4 {ECO:0000305|PubMed:2997708};
DE   AltName: Full=Endonuclease BsuRI;
DE   AltName: Full=Type-2 restriction enzyme BsuRI;
GN   Name=hsdRR; Synonyms=hsrR;
OS   Bacillus subtilis.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=R;
RX   PubMed=2997708; DOI=10.1093/nar/13.18.6403;
RA   Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J.;
RT   "Nucleotide sequence of the BsuRI restriction-modification system.";
RL   Nucleic Acids Res. 13:6403-6421(1985).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GGCC-3' and cleaves after G-2.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:2997708}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000305|PubMed:2997708};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- SUBUNIT: Monomer.
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DR   EMBL; X02988; CAA26730.1; -; Genomic_DNA.
DR   PIR; A23488; NDBSR1.
DR   AlphaFoldDB; P06529; -.
DR   REBASE; 11067; V.BsuRIP.
DR   REBASE; 620; BsuRI.
DR   PRO; PR:P06529; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Magnesium; Nuclease; Restriction system.
FT   CHAIN           1..576
FT                   /note="Type II restriction enzyme BsuRI"
FT                   /id="PRO_0000077292"
SQ   SEQUENCE   576 AA;  66304 MW;  054D1182E510E12F CRC64;
     MGKNSKAIGN NHVKSVYQAL LQSLKSKSVN GFSKITIETI SFIKNLYPEI DSVTSKFDNS
     RPDQSKDLTL YLKSGETISL NLFLIKKGRR IQPKNAGAKS FLEKYFLSAE MQKIFNKEFE
     RYYLDYLKEV VEHKKGTHYI TDKRELKRLV SSHFPKFTEE INLYRDKFLF NLRETCFTLL
     QQFYNEKNIG FTHAFNVFFM VNDTNIITSY GKDENDVKVE KFAPASPSLK DIELYKTGKS
     TVGIKFGEVG LTLRFKFESD PWKSIKLATG YHEFPKEKER VNVNLKTMRR MEKLLNKHEY
     AKTSNNSNAI GKCHEAWTYY YFLKAFPDVI QVDPKQCVEL INTYFSSINQ NTLKKLYSST
     STIVDAITEK LRQKYHDYII ESIELIPDAY IKDRLDTGDL QLVLKVNNNI IVENISLKAL
     AKRNSKITTK NPGMGSILGP TYFNMGSMES VINEVKNKFT IGEFNHRKSL EILSYEFGMK
     LDSATQEQLR RGIHNLLGKA MIAITIYGEG ISFCKEPSEI DGEVKVHVNV PSAIQNTLTW
     NNELESISLR AKFSKSQKHG WSSIKLTSEC QLESRK
//