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P06529

- T2BR_BACIU

UniProt

P06529 - T2BR_BACIU

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Protein

Type-2 restriction enzyme BsuRI

Gene

hsdRR

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 3 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence GGCC and cleaves after G-2.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Magnesium.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Protein family/group databases

REBASEi620. BsuRI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BsuRI (EC:3.1.21.4)
Short name:
R.BsuRI
Alternative name(s):
Endonuclease BsuRI
Type II restriction enzyme BsuRI
Gene namesi
Name:hsdRR
Synonyms:hsrR
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Type-2 restriction enzyme BsuRIPRO_0000077292Add
BLAST

Interactioni

Subunit structurei

Monomer.

Sequencei

Sequence statusi: Complete.

P06529-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKNSKAIGN NHVKSVYQAL LQSLKSKSVN GFSKITIETI SFIKNLYPEI
60 70 80 90 100
DSVTSKFDNS RPDQSKDLTL YLKSGETISL NLFLIKKGRR IQPKNAGAKS
110 120 130 140 150
FLEKYFLSAE MQKIFNKEFE RYYLDYLKEV VEHKKGTHYI TDKRELKRLV
160 170 180 190 200
SSHFPKFTEE INLYRDKFLF NLRETCFTLL QQFYNEKNIG FTHAFNVFFM
210 220 230 240 250
VNDTNIITSY GKDENDVKVE KFAPASPSLK DIELYKTGKS TVGIKFGEVG
260 270 280 290 300
LTLRFKFESD PWKSIKLATG YHEFPKEKER VNVNLKTMRR MEKLLNKHEY
310 320 330 340 350
AKTSNNSNAI GKCHEAWTYY YFLKAFPDVI QVDPKQCVEL INTYFSSINQ
360 370 380 390 400
NTLKKLYSST STIVDAITEK LRQKYHDYII ESIELIPDAY IKDRLDTGDL
410 420 430 440 450
QLVLKVNNNI IVENISLKAL AKRNSKITTK NPGMGSILGP TYFNMGSMES
460 470 480 490 500
VINEVKNKFT IGEFNHRKSL EILSYEFGMK LDSATQEQLR RGIHNLLGKA
510 520 530 540 550
MIAITIYGEG ISFCKEPSEI DGEVKVHVNV PSAIQNTLTW NNELESISLR
560 570
AKFSKSQKHG WSSIKLTSEC QLESRK
Length:576
Mass (Da):66,304
Last modified:January 1, 1988 - v1
Checksum:i054D1182E510E12F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02988 Genomic DNA. Translation: CAA26730.1.
PIRiA23488. NDBSR1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02988 Genomic DNA. Translation: CAA26730.1 .
PIRi A23488. NDBSR1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 620. BsuRI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the BsuRI restriction-modification system."
    Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J.
    Nucleic Acids Res. 13:6403-6421(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R.

Entry informationi

Entry nameiT2BR_BACIU
AccessioniPrimary (citable) accession number: P06529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3