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P06529

- T2BR_BACIU

UniProt

P06529 - T2BR_BACIU

Protein

Type-2 restriction enzyme BsuRI

Gene

hsdRR

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 3 out of 5- Protein predictedi
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    • History
      Entry version 64 (16 Apr 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GGCC and cleaves after G-2.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Protein family/group databases

    REBASEi620. BsuRI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme BsuRI (EC:3.1.21.4)
    Short name:
    R.BsuRI
    Alternative name(s):
    Endonuclease BsuRI
    Type II restriction enzyme BsuRI
    Gene namesi
    Name:hsdRR
    Synonyms:hsrR
    OrganismiBacillus subtilis
    Taxonomic identifieri1423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 576576Type-2 restriction enzyme BsuRIPRO_0000077292Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Sequencei

    Sequence statusi: Complete.

    P06529-1 [UniParc]FASTAAdd to Basket

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    MGKNSKAIGN NHVKSVYQAL LQSLKSKSVN GFSKITIETI SFIKNLYPEI    50
    DSVTSKFDNS RPDQSKDLTL YLKSGETISL NLFLIKKGRR IQPKNAGAKS 100
    FLEKYFLSAE MQKIFNKEFE RYYLDYLKEV VEHKKGTHYI TDKRELKRLV 150
    SSHFPKFTEE INLYRDKFLF NLRETCFTLL QQFYNEKNIG FTHAFNVFFM 200
    VNDTNIITSY GKDENDVKVE KFAPASPSLK DIELYKTGKS TVGIKFGEVG 250
    LTLRFKFESD PWKSIKLATG YHEFPKEKER VNVNLKTMRR MEKLLNKHEY 300
    AKTSNNSNAI GKCHEAWTYY YFLKAFPDVI QVDPKQCVEL INTYFSSINQ 350
    NTLKKLYSST STIVDAITEK LRQKYHDYII ESIELIPDAY IKDRLDTGDL 400
    QLVLKVNNNI IVENISLKAL AKRNSKITTK NPGMGSILGP TYFNMGSMES 450
    VINEVKNKFT IGEFNHRKSL EILSYEFGMK LDSATQEQLR RGIHNLLGKA 500
    MIAITIYGEG ISFCKEPSEI DGEVKVHVNV PSAIQNTLTW NNELESISLR 550
    AKFSKSQKHG WSSIKLTSEC QLESRK 576
    Length:576
    Mass (Da):66,304
    Last modified:January 1, 1988 - v1
    Checksum:i054D1182E510E12F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02988 Genomic DNA. Translation: CAA26730.1.
    PIRiA23488. NDBSR1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02988 Genomic DNA. Translation: CAA26730.1 .
    PIRi A23488. NDBSR1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 620. BsuRI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the BsuRI restriction-modification system."
      Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J.
      Nucleic Acids Res. 13:6403-6421(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: R.

    Entry informationi

    Entry nameiT2BR_BACIU
    AccessioniPrimary (citable) accession number: P06529
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: April 16, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3