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P06529 (T2BR_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BsuRI

Short name=R.BsuRI
EC=3.1.21.4
Alternative name(s):
Endonuclease BsuRI
Type II restriction enzyme BsuRI
Gene names
Name:hsdRR
Synonyms:hsrR
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGCC and cleaves after G-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Subunit structure

Monomer.

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Type-2 restriction enzyme BsuRI
PRO_0000077292

Sequences

Sequence LengthMass (Da)Tools
P06529 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 054D1182E510E12F

FASTA57666,304
        10         20         30         40         50         60 
MGKNSKAIGN NHVKSVYQAL LQSLKSKSVN GFSKITIETI SFIKNLYPEI DSVTSKFDNS 

        70         80         90        100        110        120 
RPDQSKDLTL YLKSGETISL NLFLIKKGRR IQPKNAGAKS FLEKYFLSAE MQKIFNKEFE 

       130        140        150        160        170        180 
RYYLDYLKEV VEHKKGTHYI TDKRELKRLV SSHFPKFTEE INLYRDKFLF NLRETCFTLL 

       190        200        210        220        230        240 
QQFYNEKNIG FTHAFNVFFM VNDTNIITSY GKDENDVKVE KFAPASPSLK DIELYKTGKS 

       250        260        270        280        290        300 
TVGIKFGEVG LTLRFKFESD PWKSIKLATG YHEFPKEKER VNVNLKTMRR MEKLLNKHEY 

       310        320        330        340        350        360 
AKTSNNSNAI GKCHEAWTYY YFLKAFPDVI QVDPKQCVEL INTYFSSINQ NTLKKLYSST 

       370        380        390        400        410        420 
STIVDAITEK LRQKYHDYII ESIELIPDAY IKDRLDTGDL QLVLKVNNNI IVENISLKAL 

       430        440        450        460        470        480 
AKRNSKITTK NPGMGSILGP TYFNMGSMES VINEVKNKFT IGEFNHRKSL EILSYEFGMK 

       490        500        510        520        530        540 
LDSATQEQLR RGIHNLLGKA MIAITIYGEG ISFCKEPSEI DGEVKVHVNV PSAIQNTLTW 

       550        560        570 
NNELESISLR AKFSKSQKHG WSSIKLTSEC QLESRK 

« Hide

References

[1]"Nucleotide sequence of the BsuRI restriction-modification system."
Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J.
Nucleic Acids Res. 13:6403-6421(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: R.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02988 Genomic DNA. Translation: CAA26730.1.
PIRNDBSR1. A23488.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE620. BsuRI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2BR_BACIU
AccessionPrimary (citable) accession number: P06529
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries