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P06493

- CDK1_HUMAN

UniProt

P06493 - CDK1_HUMAN

Protein

Cyclin-dependent kinase 1

Gene

CDK1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 192 (01 Oct 2014)
      Sequence version 3 (31 May 2011)
      Previous versions | rss
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    Functioni

    Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration.14 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it. Activated through a multistep process; binding to cyclin-B is required for relocation of cyclin-kinase complexes to the nucleus, activated by CAK/CDK7-mediated phosphorylation on Thr-161, and CDC25-mediated dephosphorylation of inhibitory phosphorylation on Thr-14 and Tyr-15. Inhibited by flavopiridol and derivatives, pyrimidine derivatives, pyridine derivatives, purine derivatives, staurosporine, paullones, oxoindoles, indazole analogs, indolin-2-ones, pyrazolo[3,4-b]pyridines, imidazo[1,2-a]pyridine (AZ703), thiazolinone analogs(RO-3306), thiazol urea, macrocyclic quinoxalin-2-one, pyrrolo[2,3-a]carbazole, pyrazolo[1,5-a]-1,3,5-triazine, pyrazolo[1,5-a]pyrimidine (Dinaciclib, SCH 727965), 2-(1-ethyl-2-hydroxyethylamino)-6-benzylamino-9-isopropylpurine (roscovitine), olomoucine, AG-024322, AT-7519, P276-00, R547/Ro-4584820 and SNS-032/BMS-387032. Repressed by the CDK inhibitors CDKN1A/p21 and CDKN1B/p27 during the G1 phase and by CDKN1A/p21 at the G1-S checkpoint upon DNA damage. Transient activation by rapid and transient dephosphorylation at Tyr-15 triggered by TGFB1.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei128 – 1281Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
    3. histone kinase activity Source: Ensembl
    4. protein binding Source: UniProtKB
    5. protein kinase activity Source: ProtInc
    6. protein serine/threonine kinase activity Source: WormBase
    7. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB

    GO - Biological processi

    1. activation of MAPK activity Source: Reactome
    2. activation of MAPKK activity Source: Reactome
    3. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: Reactome
    4. apoptotic process Source: UniProtKB-KW
    5. axon guidance Source: Reactome
    6. cell aging Source: Ensembl
    7. cell migration Source: UniProtKB
    8. cellular response to hydrogen peroxide Source: Ensembl
    9. centrosome cycle Source: UniProtKB
    10. chromosome condensation Source: Ensembl
    11. DNA repair Source: UniProtKB
    12. DNA replication Source: UniProtKB
    13. epidermal growth factor receptor signaling pathway Source: Reactome
    14. epithelial cell differentiation Source: UniProt
    15. Fc-epsilon receptor signaling pathway Source: Reactome
    16. fibroblast growth factor receptor signaling pathway Source: Reactome
    17. G1/S transition of mitotic cell cycle Source: Reactome
    18. G2/M transition of mitotic cell cycle Source: Reactome
    19. innate immune response Source: Reactome
    20. insulin receptor signaling pathway Source: Reactome
    21. MAPK cascade Source: Reactome
    22. microtubule cytoskeleton organization Source: UniProtKB
    23. mitotic cell cycle Source: Reactome
    24. mitotic G2 DNA damage checkpoint Source: Ensembl
    25. mitotic nuclear division Source: UniProtKB-KW
    26. mitotic nuclear envelope disassembly Source: Reactome
    27. MyD88-dependent toll-like receptor signaling pathway Source: Reactome
    28. MyD88-independent toll-like receptor signaling pathway Source: Reactome
    29. negative regulation of apoptotic process Source: UniProtKB
    30. neurotrophin TRK receptor signaling pathway Source: Reactome
    31. organ regeneration Source: Ensembl
    32. peptidyl-serine phosphorylation Source: WormBase
    33. positive regulation of cardiac muscle cell proliferation Source: Ensembl
    34. positive regulation of DNA replication Source: Ensembl
    35. positive regulation of gene expression Source: Ensembl
    36. positive regulation of mitotic cell cycle Source: Ensembl
    37. positive regulation of protein import into nucleus, translocation Source: Ensembl
    38. positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle Source: Reactome
    39. pronuclear fusion Source: UniProtKB
    40. protein complex assembly Source: Ensembl
    41. protein localization to kinetochore Source: BHF-UCL
    42. Ras protein signal transduction Source: Reactome
    43. regulation of embryonic development Source: UniProtKB
    44. regulation of Schwann cell differentiation Source: UniProtKB
    45. regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Reactome
    46. regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle Source: Reactome
    47. response to activity Source: Ensembl
    48. response to amine Source: Ensembl
    49. response to axon injury Source: Ensembl
    50. response to cadmium ion Source: Ensembl
    51. response to copper ion Source: Ensembl
    52. response to drug Source: Ensembl
    53. response to ethanol Source: Ensembl
    54. response to organic cyclic compound Source: Ensembl
    55. response to toxic substance Source: Ensembl
    56. small GTPase mediated signal transduction Source: Reactome
    57. stress-activated MAPK cascade Source: Reactome
    58. toll-like receptor 10 signaling pathway Source: Reactome
    59. toll-like receptor 2 signaling pathway Source: Reactome
    60. toll-like receptor 3 signaling pathway Source: Reactome
    61. toll-like receptor 4 signaling pathway Source: Reactome
    62. toll-like receptor 5 signaling pathway Source: Reactome
    63. toll-like receptor 9 signaling pathway Source: Reactome
    64. toll-like receptor signaling pathway Source: Reactome
    65. toll-like receptor TLR1:TLR2 signaling pathway Source: Reactome
    66. toll-like receptor TLR6:TLR2 signaling pathway Source: Reactome
    67. TRIF-dependent toll-like receptor signaling pathway Source: Reactome
    68. ventricular cardiac muscle cell development Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Apoptosis, Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 2681.
    ReactomeiREACT_1100. Golgi Cisternae Pericentriolar Stack Reorganization.
    REACT_111214. G0 and Early G1.
    REACT_1321. E2F-enabled inhibition of pre-replication complex formation.
    REACT_1391. ERK1 activation.
    REACT_150182. MASTL Facilitates Mitotic Progression.
    REACT_150260. Condensation of Prometaphase Chromosomes.
    REACT_150425. Resolution of Sister Chromatid Cohesion.
    REACT_15296. Recruitment of mitotic centrosome proteins and complexes.
    REACT_15364. Loss of Nlp from mitotic centrosomes.
    REACT_15451. Loss of proteins required for interphase microtubule organization from the centrosome.
    REACT_15510. Recruitment of NuMA to mitotic centrosomes.
    REACT_160122. Activation of NIMA Kinases NEK9, NEK6, NEK7.
    REACT_160315. Regulation of PLK1 Activity at G2/M Transition.
    REACT_163931. Nuclear Pore Complex (NPC) Disassembly.
    REACT_172744. Condensation of Prophase Chromosomes.
    REACT_1846. G2/M DNA replication checkpoint.
    REACT_1857. Cyclin A/B1 associated events during G2/M transition.
    REACT_200828. Depolymerisation of the Nuclear Lamina.
    REACT_2101. Cyclin B2 mediated events.
    REACT_407. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_471. E2F mediated regulation of DNA replication.
    REACT_6362. Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes.
    REACT_6820. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_683. G1/S-Specific Transcription.
    REACT_6837. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_6875. Phosphorylation of Emi1.
    REACT_6904. Phosphorylation of the APC/C.
    SignaLinkiP06493.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDK1
    Alternative name(s):
    Cell division control protein 2 homolog
    Cell division protein kinase 1
    p34 protein kinase
    Gene namesi
    Name:CDK1
    Synonyms:CDC2, CDC28A, CDKN1, P34CDC2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:1722. CDK1.

    Subcellular locationi

    Nucleus. Cytoplasm. Mitochondrion. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Cytoplasmcytoskeletonspindle
    Note: Cytoplasmic during the interphase. Colocalizes with SIRT2 on centrosome during prophase and on splindle fibers during metaphase of the mitotic cell cycle. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. cytoplasm Source: HPA
    3. cytosol Source: Reactome
    4. extracellular vesicular exosome Source: UniProt
    5. membrane Source: UniProtKB
    6. midbody Source: UniProtKB
    7. mitochondrion Source: UniProtKB
    8. mitotic spindle Source: UniProtKB
    9. nucleoplasm Source: Reactome
    10. nucleus Source: HPA
    11. spindle microtubule Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi4 – 41Y → D or E: Constitutive polyubiquitination. 2 Publications
    Mutagenesisi14 – 152TY → AF: Abnormal cell cycle exhibiting only M-phase without completing either karyokinesis or cytokinesis. 1 Publication

    Organism-specific databases

    PharmGKBiPA99.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 297297Cyclin-dependent kinase 1PRO_0000085724Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine3 Publications
    Modified residuei4 – 41Phosphotyrosine; by PKR2 Publications
    Modified residuei6 – 61N6-acetyllysine1 Publication
    Modified residuei9 – 91N6-acetyllysineBy similarity
    Modified residuei14 – 141Phosphothreonine; by PKMYT14 Publications
    Modified residuei15 – 151Phosphotyrosine; by PKMYT1, WEE1, WEE2 and PKC/PRKCD5 Publications
    Modified residuei19 – 191Phosphotyrosine3 Publications
    Modified residuei39 – 391Phosphoserine3 Publications
    Modified residuei77 – 771Phosphotyrosine2 Publications
    Cross-linki89 – 89Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)2 Publications
    Modified residuei161 – 1611Phosphothreonine; by CAK5 Publications
    Modified residuei178 – 1781Phosphoserine2 Publications
    Modified residuei222 – 2221Phosphothreonine3 Publications
    Modified residuei245 – 2451N6-succinyllysineBy similarity
    Modified residuei248 – 2481Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylation at Thr-161 by CAK/CDK7 activates kinase activity. Phosphorylation at Thr-14 and Tyr-15 by PKMYT1 prevents nuclear translocation. Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylation by PKMYT1 and WEE1 takes place during mitosis to keep CDK1-cyclin-B complexes inactive until the end of G2. By the end of G2, PKMYT1 and WEE1 are inactivated, but CDC25A and CDC25B are activated. Dephosphorylation by active CDC25A and CDC25B at Thr-14 and Tyr-15, leads to CDK1 activation at the G2-M transition. Phosphorylation at Tyr-15 by WEE2 during oogenesis is required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, leading to prevent meiotic reentry. Phosphorylation by WEE2 is also required for metaphase II exit during egg activation to ensure exit from meiosis in oocytes and promote pronuclear formation. Phosphorylated at Tyr-4 by PKR/EIF2AK2 upon genotoxic stress. This phosphorylation triggers CDK1 polyubiquitination and subsequent proteolysis, thus leading to G2 arrest. In response to UV irradiation, phosphorylation at Tyr-15 by PRKCD activates the G2/M DNA damage checkpoint.12 Publications
    Polyubiquitinated upon genotoxic stress.2 Publications

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP06493.
    PaxDbiP06493.
    PRIDEiP06493.

    2D gel databases

    SWISS-2DPAGEP06493.

    PTM databases

    PhosphoSiteiP06493.

    Expressioni

    Tissue specificityi

    Isoform 2 is found in breast cancer tissues.

    Inductioni

    Follows a cyclic expression; during interphase, accumulates gradually following G1, S to reach a critical threshold at the end of G2, which promotes self-activation and triggers onset of mitosis. Induced transiently by TGFB1 at an early phase of TGFB1-mediated apoptosis, but later repressed. Triggered by CKS1B during mitotic entry in breast cancer cells. Down-regulated under genotoxic stresses triggered by PKR/EIF2AK2-mediated phosphorylation.2 Publications

    Gene expression databases

    ArrayExpressiP06493.
    BgeeiP06493.
    CleanExiHS_CDC2.
    GenevestigatoriP06493.

    Organism-specific databases

    HPAiCAB003799.
    HPA003387.

    Interactioni

    Subunit structurei

    Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cyclins-B (CCNB1, CCNB2 and CCNB3) to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Can also form CDK1-cylin-D and CDK1-cyclin-E complexes that phosphorylate RB1 in vitro. Binds to RB1 and other transcription factors such as FOXO1 and RUNX2. Promotes G2-M transition when in complex with a cyclin-B. Interacts with DLGAP5. Binds to the CDK inhibitors CDKN1A/p21 and CDKN1B/p27. Isoform 2 is unable to complex with cyclin-B1 and also fails to bind to CDKN1A/p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase. Associates with cyclins-A and B1 during S-phase in regenerating hepatocytes. Interacts with FANCC. Interacts with CEP63; this interaction recruits CDK1 to centrosomes.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    P030702EBI-444308,EBI-617698From a different organism.
    CCNB1P146356EBI-444308,EBI-495332
    CDC25CP303072EBI-444308,EBI-974439
    EIF2AK2P195254EBI-444308,EBI-640775
    FOXO1Q127785EBI-444308,EBI-1108782
    LATS1O958352EBI-444308,EBI-444209
    PKMYT1Q996403EBI-444308,EBI-495308
    UBCP0CG486EBI-444308,EBI-3390054

    Protein-protein interaction databases

    BioGridi107420. 154 interactions.
    DIPiDIP-35N.
    IntActiP06493. 68 interactions.
    MINTiMINT-5000894.
    STRINGi9606.ENSP00000306043.

    Structurei

    Secondary structure

    1
    297
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 129
    Beta strandi14 – 2310
    Turni24 – 263
    Beta strandi29 – 368
    Beta strandi39 – 424
    Helixi46 – 5712
    Beta strandi66 – 716
    Beta strandi73 – 819
    Beta strandi84 – 863
    Helixi87 – 937
    Beta strandi94 – 963
    Helixi102 – 12120
    Beta strandi133 – 1364
    Beta strandi142 – 1443
    Helixi167 – 1693
    Helixi172 – 1765
    Helixi184 – 19916
    Helixi209 – 22012
    Turni225 – 2273
    Beta strandi234 – 2363
    Helixi249 – 2524
    Turni253 – 2553
    Helixi258 – 26710
    Turni272 – 2743
    Helixi278 – 2836
    Helixi285 – 2873

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LC9model-A1-297[»]
    ProteinModelPortaliP06493.
    SMRiP06493. Positions 1-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 287284Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG014652.
    KOiK02087.
    OMAiGKMALKH.
    PhylomeDBiP06493.
    TreeFamiTF101021.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P06493-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR    50
    EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM 100
    DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA 150
    RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK 200
    KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA 250
    SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKM 297
    Length:297
    Mass (Da):34,095
    Last modified:May 31, 2011 - v3
    Checksum:i942D79448EFE490A
    GO
    Isoform 2 (identifier: P06493-2) [UniParc]FASTAAdd to Basket

    Also known as: CDC2deltaT

    The sequence of this isoform differs from the canonical sequence as follows:
         107-163: Missing.

    Show »
    Length:240
    Mass (Da):27,503
    Checksum:i50FC3D192024E38C
    GO

    Sequence cautioni

    The sequence EAW54204.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei107 – 16357Missing in isoform 2. 1 PublicationVSP_021375Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05360 mRNA. Translation: CAA28963.1.
    Y00272 mRNA. Translation: CAA68376.1.
    D88357 mRNA. Translation: BAA26001.1.
    AK291939 mRNA. Translation: BAF84628.1.
    BT007004 mRNA. Translation: AAP35650.1.
    AF512554 Genomic DNA. Translation: AAM34793.1.
    AC022390 Genomic DNA. No translation available.
    CH471083 Genomic DNA. Translation: EAW54204.1. Sequence problems.
    BC014563 mRNA. Translation: AAH14563.1.
    CCDSiCCDS44408.1. [P06493-1]
    CCDS7260.1. [P06493-2]
    PIRiA29539.
    RefSeqiNP_001777.1. NM_001786.4. [P06493-1]
    NP_203698.1. NM_033379.4. [P06493-2]
    XP_005270360.1. XM_005270303.1. [P06493-1]
    XP_006718145.1. XM_006718082.1. [P06493-1]
    XP_006718146.1. XM_006718083.1. [P06493-1]
    UniGeneiHs.732435.

    Genome annotation databases

    EnsembliENST00000316629; ENSP00000325970; ENSG00000170312. [P06493-2]
    ENST00000373809; ENSP00000362915; ENSG00000170312. [P06493-2]
    ENST00000395284; ENSP00000378699; ENSG00000170312. [P06493-1]
    GeneIDi983.
    KEGGihsa:983.
    UCSCiuc001jld.3. human. [P06493-1]
    uc001jlg.3. human. [P06493-2]

    Polymorphism databases

    DMDMi334302921.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05360 mRNA. Translation: CAA28963.1 .
    Y00272 mRNA. Translation: CAA68376.1 .
    D88357 mRNA. Translation: BAA26001.1 .
    AK291939 mRNA. Translation: BAF84628.1 .
    BT007004 mRNA. Translation: AAP35650.1 .
    AF512554 Genomic DNA. Translation: AAM34793.1 .
    AC022390 Genomic DNA. No translation available.
    CH471083 Genomic DNA. Translation: EAW54204.1 . Sequence problems.
    BC014563 mRNA. Translation: AAH14563.1 .
    CCDSi CCDS44408.1. [P06493-1 ]
    CCDS7260.1. [P06493-2 ]
    PIRi A29539.
    RefSeqi NP_001777.1. NM_001786.4. [P06493-1 ]
    NP_203698.1. NM_033379.4. [P06493-2 ]
    XP_005270360.1. XM_005270303.1. [P06493-1 ]
    XP_006718145.1. XM_006718082.1. [P06493-1 ]
    XP_006718146.1. XM_006718083.1. [P06493-1 ]
    UniGenei Hs.732435.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LC9 model - A 1-297 [» ]
    ProteinModelPortali P06493.
    SMRi P06493. Positions 1-289.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107420. 154 interactions.
    DIPi DIP-35N.
    IntActi P06493. 68 interactions.
    MINTi MINT-5000894.
    STRINGi 9606.ENSP00000306043.

    Chemistry

    BindingDBi P06493.
    ChEMBLi CHEMBL308.
    GuidetoPHARMACOLOGYi 1961.

    PTM databases

    PhosphoSitei P06493.

    Polymorphism databases

    DMDMi 334302921.

    2D gel databases

    SWISS-2DPAGE P06493.

    Proteomic databases

    MaxQBi P06493.
    PaxDbi P06493.
    PRIDEi P06493.

    Protocols and materials databases

    DNASUi 983.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000316629 ; ENSP00000325970 ; ENSG00000170312 . [P06493-2 ]
    ENST00000373809 ; ENSP00000362915 ; ENSG00000170312 . [P06493-2 ]
    ENST00000395284 ; ENSP00000378699 ; ENSG00000170312 . [P06493-1 ]
    GeneIDi 983.
    KEGGi hsa:983.
    UCSCi uc001jld.3. human. [P06493-1 ]
    uc001jlg.3. human. [P06493-2 ]

    Organism-specific databases

    CTDi 983.
    GeneCardsi GC10P062539.
    HGNCi HGNC:1722. CDK1.
    HPAi CAB003799.
    HPA003387.
    MIMi 116940. gene.
    neXtProti NX_P06493.
    PharmGKBi PA99.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG014652.
    KOi K02087.
    OMAi GKMALKH.
    PhylomeDBi P06493.
    TreeFami TF101021.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 2681.
    Reactomei REACT_1100. Golgi Cisternae Pericentriolar Stack Reorganization.
    REACT_111214. G0 and Early G1.
    REACT_1321. E2F-enabled inhibition of pre-replication complex formation.
    REACT_1391. ERK1 activation.
    REACT_150182. MASTL Facilitates Mitotic Progression.
    REACT_150260. Condensation of Prometaphase Chromosomes.
    REACT_150425. Resolution of Sister Chromatid Cohesion.
    REACT_15296. Recruitment of mitotic centrosome proteins and complexes.
    REACT_15364. Loss of Nlp from mitotic centrosomes.
    REACT_15451. Loss of proteins required for interphase microtubule organization from the centrosome.
    REACT_15510. Recruitment of NuMA to mitotic centrosomes.
    REACT_160122. Activation of NIMA Kinases NEK9, NEK6, NEK7.
    REACT_160315. Regulation of PLK1 Activity at G2/M Transition.
    REACT_163931. Nuclear Pore Complex (NPC) Disassembly.
    REACT_172744. Condensation of Prophase Chromosomes.
    REACT_1846. G2/M DNA replication checkpoint.
    REACT_1857. Cyclin A/B1 associated events during G2/M transition.
    REACT_200828. Depolymerisation of the Nuclear Lamina.
    REACT_2101. Cyclin B2 mediated events.
    REACT_407. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_471. E2F mediated regulation of DNA replication.
    REACT_6362. Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes.
    REACT_6820. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_683. G1/S-Specific Transcription.
    REACT_6837. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_6875. Phosphorylation of Emi1.
    REACT_6904. Phosphorylation of the APC/C.
    SignaLinki P06493.

    Miscellaneous databases

    ChiTaRSi CDK1. human.
    GeneWikii Cdk1.
    Cyclin-dependent_kinase_1.
    GenomeRNAii 983.
    NextBioi 4122.
    PROi P06493.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P06493.
    Bgeei P06493.
    CleanExi HS_CDC2.
    Genevestigatori P06493.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2."
      Lee M.G., Nurse P.
      Nature 327:31-35(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "T-loop deletion of CDC2 from breast cancer tissues eliminates binding to cyclin B1 and cyclin-dependent kinase inhibitor p21."
      Ohta T., Okamoto K., Isohashi F., Shibata K., Fukuda M., Yamaguchi S., Xiong Y.
      Cancer Res. 58:1095-1098(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Mammary cancer.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. NIEHS SNPs program
      Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
      , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
      Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Skin.
    9. "Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement."
      Draetta G., Beach D.
      Cell 54:17-26(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, ASSOCIATION WITH P13.
    10. "Myt1: a membrane-associated inhibitory kinase that phosphorylates Cdc2 on both threonine-14 and tyrosine-15."
      Mueller P.R., Coleman T.R., Kumagai A., Dunphy W.G.
      Science 270:86-90(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-14 AND TYR-15 BY PKMYT1.
    11. "The Fanconi anemia polypeptide, FAC, binds to the cyclin-dependent kinase, cdc2."
      Kupfer G.M., Yamashita T., Naf D., Suliman A., Asano S., D'Andrea A.D.
      Blood 90:1047-1054(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FANCC.
    12. "Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5."
      Meijer L., Borgne A., Mulner O., Chong J.P.J., Blow J.J., Inagaki N., Inagaki M., Delcros J.-G., Moulinoux J.-P.
      Eur. J. Biochem. 243:527-536(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION BY ROSCOVITINE AND OLOMOUCINE.
    13. "RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis."
      Rosse C., L'Hoste S., Offner N., Picard A., Camonis J.
      J. Biol. Chem. 278:30597-30604(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RALBP1.
    14. "Fbx7 functions in the SCF complex regulating Cdk1-cyclin B-phosphorylated hepatoma up-regulated protein (HURP) proteolysis by a proline-rich region."
      Hsu J.-M., Lee Y.-C.G., Yu C.-T.R., Huang C.-Y.F.
      J. Biol. Chem. 279:32592-32602(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DLGAP5.
    15. "Cell cycle-dependent phosphorylation of the RUNX2 transcription factor by cdc2 regulates endothelial cell proliferation."
      Qiao M., Shapiro P., Fosbrink M., Rus H., Kumar R., Passaniti A.
      J. Biol. Chem. 281:7118-7128(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS RUNX2 KINASE.
    16. "Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1."
      Fourest-Lieuvin A., Peris L., Gache V., Garcia-Saez I., Juillan-Binard C., Lantez V., Job D.
      Mol. Biol. Cell 17:1041-1050(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS BETA-TUBULINS KINASE.
    17. "Cks1 regulates cdk1 expression: a novel role during mitotic entry in breast cancer cells."
      Westbrook L., Manuvakhova M., Kern F.G., Estes N.R. II, Ramanathan H.N., Thottassery J.V.
      Cancer Res. 67:11393-11401(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY CKS1B.
    18. "TGFbeta regulates the expression and activities of G2 checkpoint kinases in human myeloid leukemia cells."
      Hu X., Cui D., Moscinski L.C., Zhang X., Maccachero V., Zuckerman K.S.
      Cytokine 37:155-162(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS RB1 KINASE, ENZYME REGULATION BY TGFB1, REPRESSION BY TGFB1.
    19. "Microtubule deacetylases, SirT2 and HDAC6, in the nervous system."
      Southwood C.M., Peppi M., Dryden S., Tainsky M.A., Gow A.
      Neurochem. Res. 32:187-195(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS SIRT2 KINASE, SUBCELLULAR LOCATION.
    20. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
      J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    21. "Rapid cycling and precocious termination of G1 phase in cells expressing CDK1AF."
      Pomerening J.R., Ubersax J.A., Ferrell J.E. Jr.
      Mol. Biol. Cell 19:3426-3441(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION DURING THE M PHASE, MUTAGENESIS OF 14-THR-TYR-15.
    22. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-19; SER-39; TYR-77 AND THR-222, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    23. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-14 AND TYR-15, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    24. "Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells."
      Tan F., Lu L., Cai Y., Wang J., Xie Y., Wang L., Gong Y., Xu B.-E., Wu J., Luo Y., Qiang B., Yuan J., Sun X., Peng X.
      Proteomics 8:2885-2896(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-89.
      Tissue: Cervix carcinoma.
    25. "Activation of FOXO1 by Cdk1 in cycling cells and postmitotic neurons."
      Yuan Z., Becker E.B.E., Merlo P., Yamada T., DiBacco S., Konishi Y., Schaefer E.M., Bonni A.
      Science 319:1665-1668(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS FOXO1 KINASE, INTERACTION WITH FOXO1.
    26. "Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for targeting of the gammaTuRC to the centrosome."
      Zhang X., Chen Q., Feng J., Hou J., Yang F., Liu J., Jiang Q., Zhang C.
      J. Cell Sci. 122:2240-2251(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS NEDD1 KINASE.
    27. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-19; SER-39; SER-178; THR-222 AND SER-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    28. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-14; TYR-15 AND THR-161, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    29. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1 AND LYS-6, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    30. "Mitotic phosphorylation of Aki1 at Ser208 by cyclin B1-Cdk1 complex."
      Nakamura A., Naito M., Arai H., Fujita N.
      Biochem. Biophys. Res. Commun. 393:872-876(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS CC2D1A KINASE.
    31. "New Cdc2 Tyr 4 phosphorylation by dsRNA-activated protein kinase triggers Cdc2 polyubiquitination and G2 arrest under genotoxic stresses."
      Yoon C.-H., Miah M.A., Kim K.P., Bae Y.-S.
      EMBO Rep. 11:393-399(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN G2 ARREST UPON DNA DAMAGE, PHOSPHORYLATION AT TYR-4 BY PKR/EIF2AK2, POLYUBIQUITINATION, MUTAGENESIS OF TYR-4.
    32. "The protein kinase Cdelta catalytic fragment is critical for maintenance of the G2/M DNA damage checkpoint."
      LaGory E.L., Sitailo L.A., Denning M.F.
      J. Biol. Chem. 285:1879-1887(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-15.
    33. "Cdc25 phosphatases are required for timely assembly of CDK1-cyclin B at the G2/M transition."
      Timofeev O., Cizmecioglu O., Settele F., Kempf T., Hoffmann I.
      J. Biol. Chem. 285:16978-16990(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN G2-M TRANSITION, DEPHOSPHORYLATION AT THR-14 AND TYR-15 BY CDC25, PHOSPHORYLATION AT THR-161 BY CDK7/CAK, INTERACTION WITH B-CYCLIN.
    34. "Cyclin-dependent kinase 1-mediated Bcl-xL/Bcl-2 phosphorylation acts as a functional link coupling mitotic arrest and apoptosis."
      Terrano D.T., Upreti M., Chambers T.C.
      Mol. Cell. Biol. 30:640-656(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS BCL-XL/BCL2L1 KINASE, SUBCELLULAR LOCATION.
    35. "Cdk1/cyclin B1 controls Fas-mediated apoptosis by regulating caspase-8 activity."
      Matthess Y., Raab M., Sanhaji M., Lavrik I.N., Strebhardt K.
      Mol. Cell. Biol. 30:5726-5740(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS CASP8 KINASE.
    36. "Cyclin-dependent kinases regulate epigenetic gene silencing through phosphorylation of EZH2."
      Chen S., Bohrer L.R., Rai A.N., Pan Y., Gan L., Zhou X., Bagchi A., Simon J.A., Huang H.
      Nat. Cell Biol. 12:1108-1114(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS EZH2 KINASE.
    37. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    38. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    39. "Cep63 recruits Cdk1 to the centrosome: implications for regulation of mitotic entry, centrosome amplification, and genome maintenance."
      Loffler H., Fechter A., Matuszewska M., Saffrich R., Mistrik M., Marhold J., Hornung C., Westermann F., Bartek J., Kramer A.
      Cancer Res. 71:2129-2139(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CEP63, SUBCELLULAR LOCATION.
    40. "CAMP (C13orf8, ZNF828) is a novel regulator of kinetochore-microtubule attachment."
      Itoh G., Kanno S., Uchida K.S., Chiba S., Sugino S., Watanabe K., Mizuno K., Yasui A., Hirota T., Tanaka K.
      EMBO J. 30:130-144(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS CHAMP1 KINASE.
    41. Cited for: REVIEW ON SUBSTRATES, GENE FAMILY.
    42. "The decision to enter mitosis: feedback and redundancy in the mitotic entry network."
      Lindqvist A., Rodriguez-Bravo V., Medema R.H.
      J. Cell Biol. 185:193-202(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON SUBCELLULAR TRANSLOCATION.
    43. "Cell cycle, CDKs and cancer: a changing paradigm."
      Malumbres M., Barbacid M.
      Nat. Rev. Cancer 9:153-166(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON CELL CYCLE CONTROL AND INHIBITORS, GENE FAMILY.
    44. "Cdc2: a monopotent or pluripotent CDK?"
      Hu X., Moscinski L.C.
      Cell Prolif. 44:205-211(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON CELL CYCLE CONTROL.
    45. "Cyclin dependent kinase 1 inhibitors: a review of recent progress."
      Wang Q., Su L., Liu N., Zhang L., Xu W., Fang H.
      Curr. Med. Chem. 18:2025-2043(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON CELL CYCLE CONTROL AND INHIBITORS.
    46. "Checkpoint recovery in cells: how a molecular understanding can help in the fight against cancer."
      Medema R.H., Macurek L.
      F1000 Biol. Rep. 3:10-10(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON CELL CYCLE CONTROL.
    47. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    48. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCDK1_HUMAN
    AccessioniPrimary (citable) accession number: P06493
    Secondary accession number(s): A8K7C4, C9J497, O60764
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: May 31, 2011
    Last modified: October 1, 2014
    This is version 192 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    As a key regulator of the cell cycle, CDK1 is a potent therapeutic target for inhibitors in cancer treatment.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3