P06491 (MCP_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Major capsid protein
Short name=MCP

Alternative name(s):
Capsid protein VP5

Gene names

Name:

UL19

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1374 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Self-assembles to form an icosahedral capsid with a T=16 symmetry, about 200 nm in diameter, and consisting of 150 hexons and 12 pentons (total of 162 capsomers). Hexons form the edges and faces of the capsid and are each composed of six MCP molecules. In contrast, one penton is found at each of the 12 vertices. Eleven of the pentons are MCP pentamers, while the last vertex is occupied by the portal complex. The capsid is surrounded by a layer of proteinaceous material designated the tegument which, in turn, is enclosed in an envelope of host cell-derived lipids containing virus-encoded glycoproteins. Ref.4
Subunit structure	Homomultimer. Makes the hexons and eleven out of twelve pentons. Interacts with VP19C and VP23; Adjacent capsomers are linked together in groups of three by triplexes, heterotrimeric complexes composed of one molecule of VP19C and two molecules of VP23. Interacts with VP22A; this interaction allows efficient MCP transport to the host nucleus. Interacts with VP26. Interacts with virion-packaging protein UL25. Ref.6 Ref.7 Ref.8 Ref.9
Subcellular location	Virion. Host nucleus Ref.5.
Sequence similarities	Belongs to the herpesviridae major capsid protein family.

Ontologies

Keywords
Cellular component	Host nucleus Virion
Molecular function	Capsid protein T=16 icosahedral capsid protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	T=16 icosahedral viral capsid Inferred from electronic annotation. Source: UniProtKB-KW host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1374

1374

Major capsid protein

PRO_0000115702

Secondary structure

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1374

Helix Strand Turn

Details...

Helix

487 – 489

3

Helix

498 – 506

9

Helix

510 – 514

5

Beta strand

548 – 550

3

Beta strand

572 – 574

3

Helix

579 – 581

3

Turn

584 – 586

3

Helix

589 – 600

12

Helix

608 – 619

12

Helix

626 – 633

8

Helix

638 – 643

6

Helix

645 – 658

14

Helix

668 – 676

9

Helix

679 – 681

3

Helix

684 – 706

23

Helix

714 – 716

3

Helix

719 – 723

5

Beta strand

737 – 739

3

Helix

740 – 746

7

Turn

750 – 752

3

Beta strand

764 – 767

4

Beta strand

771 – 773

3

Beta strand

782 – 784

3

Beta strand

789 – 793

5

Beta strand

796 – 798

3

Helix

803 – 814

12

Helix

816 – 821

6

Beta strand

826 – 830

5

Helix

832 – 839

8

Turn

857 – 859

3

Helix

867 – 869

3

Helix

875 – 881

7

Helix

888 – 892

5

Helix

893 – 898

6

Beta strand

907 – 912

6

Beta strand

928 – 939

12

Turn

948 – 952

5

Beta strand

953 – 957

5

Helix

967 – 971

5

Turn

978 – 980

3

Helix

981 – 984

4

Helix

992 – 994

3

Helix

997 – 999

3

Beta strand

1000 – 1002

3

Helix

1004 – 1012

9

Helix

1017 – 1027

11

Helix

1033 – 1042

10

Sequences

	Sequence		Length	Mass (Da)
	P06491 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 859C76E2EADE05B7	FASTA	1,374	149,084
	10 20 30 40 50 60 MAAPNRDPPG YRYAAAMVPT GSLLSTIEVA SHRRLFDFFS RVRSDANSLY DVEFDALLGS 70 80 90 100 110 120 YCNTLSLVRF LELGLSVACV CTKFPELAYM NEGRVQFEVH QPLIARDGPH PIEQPTHNYM 130 140 150 160 170 180 TKIIDRRALN AAFSLATEAI ALLTGEALDG TGIGAHRQLR AIQQLARNVQ AVLGAFERGT 190 200 210 220 230 240 ADQMLHVLLE KAPPLALLLP MQRYLDNGRL ATRVARATLV AELKRSFCET SFFLGKAGHR 250 260 270 280 290 300 REAVEAWLVD LTTATQPSVA VPRLTHADTR GRPVDGVLVT TAPIKQRLLQ SFLKVEDTEA 310 320 330 340 350 360 DVPVTYGEMV LNGANLVTAL VMGKAVRSLD DVGRHLLEMQ EEQLDLNRQT LDELESAPQT 370 380 390 400 410 420 TRVRADLVSI GEKLVFLEAL EKRIYAATNV PYPLVGAMDL TFVLPLGLFN PVMERFAAHA 430 440 450 460 470 480 GDLVPAPGHP DPRAFPPRQL FFWGKDRQVL RLSLEHAIGT VCHPSLMNVD AAVGGLNRDP 490 500 510 520 530 540 VEAANPYGAY VAAPAGPAAD MQQLFLNAWG QRLAHGRVRW VAEGQMTPEQ FMQPDNANLA 550 560 570 580 590 600 LELHPAFDFF VGVADVELPG GDVPPAGPGE IQATWRVVNG NLPLALCPAA FRDARGLELG 610 620 630 640 650 660 VGRHAMAPAT IAAVRGAFDD RNYPAVFYLL QAAIHGSEHV FCALARLVVQ CITSYWNNTR 670 680 690 700 710 720 CAAFVNDYSL VSYVVTYLGG DLPEECMAVY RDLVAHVEAL AQLVDDFTLT GPELGGQAQA 730 740 750 760 770 780 ELNHLMRDPA LLPPLVWDCD ALMRRAALDR HRDCRVSAGG HDPVYAAACN VATADFNRND 790 800 810 820 830 840 GQLLHNTQAR AADAADDRPH RGADWTVHHK IYYYVMVPAF SRGRCCTAGV RFDRVYATLQ 850 860 870 880 890 900 NMVVPEIAPG EECPSDPVTD PAHPLHPANL VANTVNAMFH NGRVVVDGPA MLTLQVLAHN 910 920 930 940 950 960 MAERTTALLC SAAPDAGANT ASTTNMRIFD GALHAGILLM APQHLDHTIQ NGDYFYPLPV 970 980 990 1000 1010 1020 HALFAGADHV ANAPNFPPAL RDLSRQVPLV PPALGANYFS SIRQPVVQHV RESAAGENAL 1030 1040 1050 1060 1070 1080 TYALMAGYFK ISPVALHHQL KTGLHPGFGF TVVRQDRFVT ENVLFSERAS EAYFLGQLQV 1090 1100 1110 1120 1130 1140 ARHETGGGVN FTLTQPRANV DLGVGYTAVV ATATVRNPVT DMGNLPQNFY LGRGAPPLLD 1150 1160 1170 1180 1190 1200 NAAAVYLRNA VVAGNRLGPA QPVPVFGCAQ VPRRAGMDHG QDAVCEFIAT PVSTDVNYFR 1210 1220 1230 1240 1250 1260 RPCNPRGRAA GGVYAGDKEG DVTALMYDHG QSDPSRAFAA TANPWASQRF SYGDLLYNGA 1270 1280 1290 1300 1310 1320 YHLNGASPVL SPCFKFFTSA DIAAKHRCLE RLIVETGSAV STATAASDVQ FKRPPGCREL 1330 1340 1350 1360 1370 VEDPCGLFQE AYPLTCASDP ALLRSARNGE AHARETHFAQ YLVYDASPLK GLAL

References

[1]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence of the major capsid protein gene of herpes simplex virus type 1." Davison B.A.J., Scott J.E. J. Gen. Virol. 67:2279-2286(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Identification of genes encoding two capsid proteins (VP24 and VP26) of herpes simplex virus type 1." Davison M.D., Rixon F.J., Davison A.J. J. Gen. Virol. 73:2709-2713(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 202-211 AND 607-616.
[4]	"Structure of the herpes simplex virus capsid. Molecular composition of the pentons and the triplexes." Newcomb W.W., Trus B.L., Booy F.P., Steven A.C., Wall J.S., Brown J.C. J. Mol. Biol. 232:499-511(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Localization of the herpes simplex virus type 1 major capsid protein VP5 to the cell nucleus requires the abundant scaffolding protein VP22a." Nicholson P., Addison C., Cross A.M., Kennard J., Preston V.G., Rixon F.J. J. Gen. Virol. 75:1091-1099(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Identification of a minimal hydrophobic domain in the herpes simplex virus type 1 scaffolding protein which is required for interaction with the major capsid protein." Hong Z., Beaudet-Miller M., Durkin J., Zhang R., Kwong A.D. J. Virol. 70:533-540(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VP22A.
[7]	"Multiple interactions control the intracellular localization of the herpes simplex virus type 1 capsid proteins." Rixon F.J., Addison C., McGregor A., Macnab S.J., Nicholson P., Preston V.G., Tatman J.D. J. Gen. Virol. 77:2251-2260(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VP19C AND VP23.
[8]	"Role of the UL25 gene product in packaging DNA into the herpes simplex virus capsid: location of UL25 product in the capsid and demonstration that it binds DNA." Ogasawara M., Suzutani T., Yoshida I., Azuma M. J. Virol. 75:1427-1436(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL25.
[9]	"Residues of VP26 of herpes simplex virus type 1 that are required for its interaction with capsids." Desai P., Akpa J.C., Person S. J. Virol. 77:391-404(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VP26. Strain: KOS.
[10]	"Structure of the herpesvirus major capsid protein." Bowman B.R., Baker M.L., Rixon F.J., Chiu W., Quiocho F.A. EMBO J. 22:757-765(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 451-1054.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X14112 Genomic DNA. Translation: CAA32332.1.
X04467 Genomic DNA. Translation: CAA28154.1.

PIR

VCBE17. A27239.

RefSeq

NP_044620.1. NC_001806.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NO7	X-ray	2.90	A/B	451-1054	[»]

ProteinModelPortal

P06491.

SMR

P06491. Positions 484-1045.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-6732551.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2703368.

Phylogenomic databases

ProtClustDB

CLSP2509619.

Family and domain databases

InterPro

IPR000912. Herpes_MCP.
IPR023233. Herpes_MCP_upper.
[Graphical view]

Pfam

PF03122. Herpes_MCP. 1 hit.
[Graphical view]

PRINTS

PR00235. HSVCAPSIDMCP.

SUPFAM

SSF103417. Herpes_MCP_upper. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P06491.

Entry information

Entry name

MCP_HHV11

Accession

Primary (citable) accession number: P06491

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program