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Protein

Envelope glycoprotein I

Gene

gI

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips.
The heterodimer gE/gI serves as a receptor for the Fc part of human IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-HSV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host Ig-mediated immune responses.

GO - Biological processi

  1. evasion or tolerance by virus of host immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein I
Short name:
gI
Gene namesi
Name:gI
ORF Names:US7
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009294 Componenti: Genome

Subcellular locationi

Virion membrane; Single-pass membrane protein. Host cell membrane Curated; Single-pass type I membrane protein Curated. Host cell junction. Host Golgi apparatus membrane; Single-pass type I membrane protein
Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN). The heterodimer gE/gI then redistribute to cell junctions to promote cell-cell spread later in the infection.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 276256Virion surfaceSequence AnalysisAdd
BLAST
Transmembranei277 – 29721HelicalSequence AnalysisAdd
BLAST
Topological domaini298 – 39093IntravirionSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell Golgi membrane Source: UniProtKB-SubCell
  2. host cell junction Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. viral envelope Source: UniProtKB-KW
  5. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell junction, Host cell membrane, Host Golgi apparatus, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 390370Envelope glycoprotein IPRO_0000115770Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi156 – 1561N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi175 – 1751N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi257 – 2571N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Interacts with gE; this interaction enhances the Fc receptor function of gE.By similarity

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR002874. Herpes_gI.
[Graphical view]
PfamiPF01688. Herpes_gI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPCRPLQGLV LVGLWVCATS LVVRGPTVSL VSNSFVDAGA LGPDGVVEED
60 70 80 90 100
LLILGELRFV GDQVPHTTYY DGGVELWHYP MGHKCPRVVH VVTVTACPRR
110 120 130 140 150
PAVAFALCRA TDSTHSPAYP TLELNLAQQP LLRVQRATRD YAGVYVLRVW
160 170 180 190 200
VGDAPNASLF VLGMAIAAEG TLAYNGSAYG SCDPKLLPSS APRLAPASVY
210 220 230 240 250
QPAPNQASTP STTTSTPSTT IPAPSTTIPA PQASTTPFPT GDPKPQPPGV
260 270 280 290 300
NHEPPSNATR ATRDSRYALT VTQIIQIAIP ASIIALVFLG SCICFIHRCQ
310 320 330 340 350
RRYRRSRRPI YSPQMPTGIS CAVNEAAMAR LGAELKSHPS TPPKSRRRSS
360 370 380 390
RTPMPSLTAI AEESEPAGAA GLPTPPVDPT TPTPTPPLLV
Length:390
Mass (Da):41,370
Last modified:December 31, 1987 - v1
Checksum:i39381B1D6B5F08C8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti73 – 731G → V in strain: Nonneuroinvasive mutant HF10.
Natural varianti165 – 1651A → T in strain: Nonneuroinvasive mutant HF10.
Natural varianti225 – 2317Missing in strain: Nonneuroinvasive mutant HF10.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32284.1.
X02138 Genomic DNA. Translation: CAA26061.1.
L00036 Genomic DNA. Translation: AAA96681.1.
DQ889502 Genomic DNA. Translation: ABI63525.1.
FJ593289 Genomic DNA. Translation: ACM62296.1.
PIRiA05243. QQBE77.
RefSeqiNP_044669.1. NC_001806.1.

Genome annotation databases

GeneIDi2703446.
KEGGivg:2703446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32284.1.
X02138 Genomic DNA. Translation: CAA26061.1.
L00036 Genomic DNA. Translation: AAA96681.1.
DQ889502 Genomic DNA. Translation: ABI63525.1.
FJ593289 Genomic DNA. Translation: ACM62296.1.
PIRiA05243. QQBE77.
RefSeqiNP_044669.1. NC_001806.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL2364696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703446.
KEGGivg:2703446.

Family and domain databases

InterProiIPR002874. Herpes_gI.
[Graphical view]
PfamiPF01688. Herpes_gI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dolan A., Donald S., Rixon F.J.
    J. Mol. Biol. 181:1-13(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
    Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
    Microbes Infect. 9:142-149(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nonneuroinvasive mutant HF10.
  3. "Herpes simplex virus type 1 bacterial artificial chromosome."
    Cunningham C., Davison A.J.
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 17 syn+.
  4. "Herpes simplex virus immunoglobulin G Fc receptor activity depends on a complex of two viral glycoproteins, gE and gI."
    Johnson D.C., Frame M.C., Ligas M.W., Cross A.M., Stow N.D.
    J. Virol. 62:1347-1354(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GLYCOPROTEIN E.
    Strain: 17 syn+ and F.
  5. "pH dependence and stoichiometry of binding to the Fc region of IgG by the herpes simplex virus Fc receptor gE-gI."
    Sprague E.R., Martin W.L., Bjorkman P.J.
    J. Biol. Chem. 279:14184-14193(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: KOS.
  6. "Herpes simplex virus gE/gI must accumulate in the trans-Golgi network at early times and then redistribute to cell junctions to promote cell-cell spread."
    Farnsworth A., Johnson D.C.
    J. Virol. 80:3167-3179(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: F.
  7. "Comprehensive characterization of extracellular herpes simplex virus type 1 virions."
    Loret S., Guay G., Lippe R.
    J. Virol. 82:8605-8618(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: F.

Entry informationi

Entry nameiGI_HHV11
AccessioniPrimary (citable) accession number: P06487
Secondary accession number(s): B9VQK1, Q09I70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 31, 1987
Last sequence update: December 31, 1987
Last modified: March 31, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.