ID   US9_HHV11               Reviewed;          90 AA.
AC   P06481; B9VQK4; Q09I67;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   24-JAN-2024, entry version 102.
DE   RecName: Full=Envelope protein US9;
DE   AltName: Full=10 kDa protein;
GN   ORFNames=US9;
OS   Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Orthoherpesviridae; Alphaherpesvirinae; Simplexvirus;
OC   Simplexvirus humanalpha1; Human herpesvirus 1.
OX   NCBI_TaxID=10299;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2984429; DOI=10.1016/0022-2836(85)90320-1;
RA   McGeoch D.J., Dolan A., Donald S., Rixon F.J.;
RT   "Sequence determination and genetic content of the short unique region in
RT   the genome of herpes simplex virus type 1.";
RL   J. Mol. Biol. 181:1-13(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2839594; DOI=10.1099/0022-1317-69-7-1531;
RA   McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D.,
RA   Perry L.J., Scott J.E., Taylor P.;
RT   "The complete DNA sequence of the long unique region in the genome of
RT   herpes simplex virus type 1.";
RL   J. Gen. Virol. 69:1531-1574(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nonneuroinvasive mutant HF10;
RX   PubMed=17218138; DOI=10.1016/j.micinf.2006.10.019;
RA   Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.;
RT   "Determination and analysis of the DNA sequence of highly attenuated herpes
RT   simplex virus type 1 mutant HF10, a potential oncolytic virus.";
RL   Microbes Infect. 9:142-149(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=17 syn+;
RA   Cunningham C., Davison A.J.;
RT   "Herpes simplex virus type 1 bacterial artificial chromosome.";
RL   Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=F;
RX   PubMed=17382478; DOI=10.1016/j.neuroscience.2007.02.010;
RA   LaVail J.H., Tauscher A.N., Sucher A., Harrabi O., Brandimarti R.;
RT   "Viral regulation of the long distance axonal transport of herpes simplex
RT   virus nucleocapsid.";
RL   Neuroscience 146:974-985(2007).
RN   [6]
RP   FUNCTION.
RC   STRAIN=F;
RX   PubMed=18753205; DOI=10.1128/jvi.01241-08;
RA   Snyder A., Polcicova K., Johnson D.C.;
RT   "Herpes simplex virus gE/gI and US9 proteins promote transport of both
RT   capsids and virion glycoproteins in neuronal axons.";
RL   J. Virol. 82:10613-10624(2008).
RN   [7]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=F;
RX   PubMed=18596102; DOI=10.1128/jvi.00904-08;
RA   Loret S., Guay G., Lippe R.;
RT   "Comprehensive characterization of extracellular herpes simplex virus type
RT   1 virions.";
RL   J. Virol. 82:8605-8618(2008).
RN   [8]
RP   TOPOLOGY, AND SUBCELLULAR LOCATION.
RX   PubMed=19420087; DOI=10.1128/jvi.00598-09;
RA   Lyman M.G., Kemp C.D., Taylor M.P., Enquist L.W.;
RT   "Comparison of the pseudorabies virus Us9 protein with homologs from other
RT   veterinary and human alphaherpesviruses.";
RL   J. Virol. 83:6978-6986(2009).
CC   -!- FUNCTION: Essential for the anterograde spread of the infection
CC       throughout the host nervous system. Together with the gE/gI
CC       heterodimer, US9 is involved in the sorting and transport of viral
CC       structural components toward axon tips. {ECO:0000269|PubMed:17382478,
CC       ECO:0000269|PubMed:18753205}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane; Single-pass type II membrane
CC       protein. Host Golgi apparatus membrane; Single-pass type II membrane
CC       protein. Host smooth endoplasmic reticulum membrane {ECO:0000305};
CC       Single-pass type II membrane protein {ECO:0000305}. Host cell membrane
CC       {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC       Note=During virion morphogenesis, this protein probably accumulates in
CC       the endosomes and trans-Golgi where secondary envelopment occurs. It is
CC       probably transported to the cell surface from where it is endocytosed
CC       and directed to the trans-Golgi network (TGN), maybe through an
CC       interaction with PACS-1 sorting protein (Potential). {ECO:0000305}.
CC   -!- PTM: Phosphorylated on serines within the acidic cluster, possibly by
CC       host CK2. Phosphorylation determines whether endocytosed viral US9
CC       traffics to the trans-Golgi network or recycles to the cell membrane.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the alphaherpesvirinae envelope protein US9
CC       family. {ECO:0000305}.
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DR   EMBL; L00036; AAA96679.1; -; Genomic_DNA.
DR   EMBL; X14112; CAA32274.1; -; Genomic_DNA.
DR   EMBL; X02138; CAA26063.1; -; Genomic_DNA.
DR   EMBL; DQ889502; ABI63528.1; -; Genomic_DNA.
DR   EMBL; FJ593289; ACM62299.1; -; Genomic_DNA.
DR   PIR; A05241; QQBE79.
DR   RefSeq; YP_009137145.1; NC_001806.2.
DR   SMR; P06481; -.
DR   DNASU; 2703452; -.
DR   GeneID; 2703452; -.
DR   KEGG; vg:2703452; -.
DR   Proteomes; UP000009294; Genome.
DR   Proteomes; UP000180652; Genome.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044171; C:host cell smooth endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0075733; P:intracellular transport of virus; IMP:CACAO.
DR   InterPro; IPR009278; Herpes_US9.
DR   Pfam; PF06072; Herpes_US9; 1.
PE   1: Evidence at protein level;
KW   Host cell membrane; Host endoplasmic reticulum; Host Golgi apparatus;
KW   Host membrane; Membrane; Phosphoprotein; Reference proteome; Signal-anchor;
KW   Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT   CHAIN           1..90
FT                   /note="Envelope protein US9"
FT                   /id="PRO_0000116132"
FT   TOPO_DOM        1..67
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        68..88
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        89..90
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000250"
FT   REGION          30..39
FT                   /note="Acidic"
FT   MOTIF           21..24
FT                   /note="Internalization motif"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         34
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         36
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
FT   VARIANT         29
FT                   /note="V -> A (in strain: Nonneuroinvasive mutant HF10)"
SQ   SEQUENCE   90 AA;  10027 MW;  F4D4DC2608BB38F7 CRC64;
     MTSRLSDPNS SARSDMSVPL YPTASPVSVE AYYSESEDEA ANDFLVRMGR QQSVLRRRRR
     RTRCVGMVIA CLLVAVLSGG FGALLMWLLR
//